FastQCFastQC Report
Sat 31 Dec 2022
SRR5846287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159155
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT9390.5899908893845622RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8950.5623448839181929No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2250.14137161886211555No Hit
ATGGCTGGCGATCTACATCCGGGGGGATACAAGATGAATCATGGAGGGTTGG2080.13069020765920017No Hit
GTCTCGGGGTGATAAAGTCTTAGACAGTTGAAAGACAATTGCTCAATGACCT1940.1218937513744463No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1820.11435393170180014No Hit
GCCACTGGGGGAATTTTGACAGTGAATACATTGAATCTGTAGATAAATTAGG1740.10932738525336937No Hit
CTATTTGGGATTTCTGCCTCGTGGCCTACACCTCCTAGCATAGTAAGTGCTC1680.10555747541704627No Hit
GAAAATGGGCATGTAAAAGATAAGATATCATAACCAAGAAGCATTTATCTCA1660.10430083880493858No Hit
GAATACGGGGAGTGGTTGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTT1600.10053092896861551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)