FastQCFastQC Report
Sat 31 Dec 2022
SRR5846286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158863
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT9300.5854100703121558RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8140.5123911798216073No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC2250.14163146862390866No Hit
ATGGCTGGCGATCTACATCCGGGGGGATACAAGATGAATCATGGAGGGTTGG1920.12085885322573539No Hit
GCCACTGGGGGAATTTTGACAGTGAATACATTGAATCTGTAGATAAATTAGG1830.11519359448077904No Hit
GCTGGCGGGGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCT1720.1082693893480546No Hit
TTCATGGGAGACGAGGGCGGCGAGGTCGGGTTCCGGGCGCTTGGAGAAGATG1710.10763991615417058No Hit
GAAAATGGGCATGTAAAAGATAAGATATCATAACCAAGAAGCATTTATCTCA1680.10575149657251845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)