FastQCFastQC Report
Sat 31 Dec 2022
SRR5846285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157257
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT9000.5723115664167573RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC8210.5220753289201753No Hit
ATGGCTGGCGATCTACATCCGGGGGGATACAAGATGAATCATGGAGGGTTGG2140.13608297245909562No Hit
CGTGTCGGGAAGTCAGTTTATAAGAAAAATAAAATAATTCTGAATTTACATG1830.11637001850474063No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1790.11382641154288839No Hit
GCCACTGGGGGAATTTTGACAGTGAATACATTGAATCTGTAGATAAATTAGG1750.11128280458103613No Hit
GCTGGCGGGGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCT1720.10937509935964695No Hit
GAAAATGGGCATGTAAAAGATAAGATATCATAACCAAGAAGCATTTATCTCA1670.10619559065733163No Hit
TGCTCAGGGAGGCAAAAATGAAACTAAATGAAAGCGAAAGGGAAAATAAAGA1620.10301608195501633No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1620.10301608195501633No Hit
AACGTTGGGAGATTGGCAGTCTCTTTCGCTTATCTGCTGTTTTAAAGAATTC1580.10047247499316404No Hit
GAATTGGGGCTATGTGTGGATGAGAAATAAACATTCATTAGATGAAGCAACT1580.10047247499316404No Hit
ATTAGAGGGGGGATAGCACCAAACCTTTCAATTGGGAAAACAAAATCTAATT1580.10047247499316404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)