FastQCFastQC Report
Sat 31 Dec 2022
SRR5846271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130498
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT8000.6130362151144079RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC6070.4651412282180569No Hit
TAGCTAGGGTACCGCACAGGCATGCTGCAGTGAATTTAACTGATCCTCTGTC1860.14253092001409984No Hit
GTTTAAGGGTGCTAGCCACATGTGGCTATTTAAATTTAGTTAAAATGGAATA1810.13869944366963477No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1800.13793314840074178No Hit
CAGCCAGGGTAATTTATAGATTCAAGGCCATCCCCTTAAGCTACCAATGACC1790.13716685313184876No Hit
TCTCAGGGGGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTA1780.13640055786295577No Hit
AATACCGGGGTCTGGAAAACCTGTGTGATCGGCACAAGTACAGCTGCCCACC1770.13563426259406275No Hit
TTATGAGGGATGGCAAGAAAATACTGAATGCACTGCCATAATGTAAATATGA1760.13486796732516973No Hit
TCCACAGGGGGAGGAGGAGGGTCGGGGACGGAAGCGCAGAGCACGGACCCCG1700.13027019571181167No Hit
TGCCTTGGGGGCACACATCAAAAGACAGCAAGGCAGCAAAGAAGAAGAAAAA1700.13027019571181167No Hit
TCGCTAGGGAGCTTAGGTGATAAAATGAATGTACACCTTCCCACTTATTAAC1690.12950390044291865No Hit
TCCACTGGGGGTATTTGGTTAAAGGTCTCCCACAAGACTGGTATTCTCTTTG1640.1256724240984536No Hit
TACTATGGGAAGAAAATACTGTAAACAACTTTACATACATGATAGTTTAAGA1610.12337353829177458No Hit
TTTTAGGGGGTGGACCTACATGCGGAGTGCGGAGCCCTCTGTGTTTGTGAGG1590.12184094775398856No Hit
TTACATGGGAGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCT1590.12184094775398856No Hit
TGCTGGGGGCTCTGGAAAAAGTACTCATCCTTTCCTGAATTCAGAGCCCTTC1580.12107465248509557No Hit
ACAGTTGGGTAACCTATAACAAGGAGACATTACATTTTACTTTAGAACATGA1560.11954206194730954No Hit
ATGTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1550.11877576667841652No Hit
TTTGCCGGTCCAGCAGCAGAGTAGACAACGCTCCCGACCAGCAGAACTCCCA1540.11800947140952352No Hit
CTAAATGGGAGGAGAACATTTGTAAGGGTAGAATATTTGTTTTCAGAGATGT1540.11800947140952352No Hit
TGAAATGGGATCAAAATCATAGCTGGCTCTTTTCCAGGAAATAAATAAGCTG1510.11571058560284449No Hit
AGCTTCGGGCAGCAATTATTTTTATTGTCTTCAGAACTTCTTTTTGCAAGGA1510.11571058560284449No Hit
TTCGTCGGGACAAGCAGTGAATGACTGCATTTGAAATATAAAACACAAAGTC1500.11494429033395148No Hit
AACCTTGGGACATGGGGGTGGAATGGCTGGATCATATGGTAGGTGTATGTTT1490.11417799506505846No Hit
GCTACTGGGAGTTACAGACATCCATACTCACTTTTTAAAATAACTTTTTAAA1480.11341169979616546No Hit
TACCAAGGGGAGCCTCGCTTTTTCATCCTTCTTTCCTTGTTCAGCAGGCTAG1470.11264540452727244No Hit
AAAGCAGGGAACTCTTTTGCAAAATGAGAACAATTAGATCATATTTTCATGG1460.11187910925837945No Hit
CGCGTGGGCGTACTGAAACATCGTCTTTACCCACTTCTCAGTTTATAATGGG1460.11187910925837945No Hit
CGATTAGGGGAAGAAATGTGACTTTTGTAAGATTATTTCTCTTGTTATTGTC1460.11187910925837945No Hit
TCAACGGGGGGGATTCAAATTCAGTCCACAATGCAGTTGATTTGTATGTCTC1460.11187910925837945No Hit
ACGTCAGGGCTGGAGATGAGATGACATCTCTGTCAGAGTATGTTTCTCGCAT1450.11111281398948641No Hit
TTGCCAGGGCTGAAAACCGAAAGTCAATTCTAATTATTCATCTTACATATTT1450.11111281398948641No Hit
TGTCACGGGGTTTGGGTCACTTCATTATGTTGACAAATTCCTTATTTGATAT1440.11034651872059342No Hit
GCACAGGGAATGCTTAAGTGTTCTGGTTATGCCATTCATTCATACGATGTGT1430.1095802234517004No Hit
TGCTGGGGGTTAATGGAAAACTTTTTCTAATCAAGTCAGGTGAATGTGTATT1410.10804763291391438No Hit
AGCTATGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1410.10804763291391438No Hit
CATTGCGGGTAACCCATGGGTTAAAGAAGAAATCAAAAGAAAAAATTACAAG1410.10804763291391438No Hit
ACGTGGGGGATGACAGCTTTGTGGAGAAATGAAAACATTTGGAAAATAGTGT1410.10804763291391438No Hit
GTCCAAGGGGGGAGGGCAGAAGGCAGAGTCCAGGCTTAGACTGCAGTTCCTC1400.10728133764502139No Hit
AGTCTAGGGGCACATGAAAAAATTAATTCACCGCAATCAAGTGGGTTTCATT1400.10728133764502139No Hit
GCTGCTGGGATTGCAGGCGTGAGCCACCACGCCCAGCCACACACATTTTTTT1400.10728133764502139No Hit
TGTTAGGGACCTCATCGGCATTGCTGCCATCGTGGTCGCTGTGATCATGATC1400.10728133764502139No Hit
GAGGACGGGATTTATGAAGGTTGCACATATACATGGGAATTTTTTAGTCTGG1390.10651504237612835No Hit
TGCAAAGGGAGCAAATTTAAGTGTTTCTGTCTCTGCTCCATTCCTTTCAACT1390.10651504237612835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1390.10651504237612835No Hit
TGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG1380.10574874710723536No Hit
ACGTCGGGTATTTATGCCACCAGTAATTTTATAAGAAATCTGAATAGTTCTC1380.10574874710723536No Hit
CTAATGGGCCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCT1380.10574874710723536No Hit
CAAACGGGGTCAGCTTTACACTTCACAGGAGATTGATGGCATTGAGGAAGAT1380.10574874710723536No Hit
GCCAATGGGTTGCCAATTATATGTCTGGGTTTCTCTCCTTATTAGCTACAGG1380.10574874710723536No Hit
GTGGCCGGGAGAGCCCGGCGGCGGCGAAGCAGCAGCTGCGGCCGCGCCCTTG1380.10574874710723536No Hit
CTTCAAGGGTATGTAATTAATTTCCTTTTAATATTTGTATTAATAAATTTAT1370.10498245183834234No Hit
AATGCAGGGTGAATATTCTTGGTCATCTCGACTAATTCTGAGGCAATGATGG1370.10498245183834234No Hit
GACTAGCGGGATTGAAAGAAATATATTCTAACAGTACGCACTGAATAGTGAA1360.10421615656944934No Hit
TTGGCTGGGCAGCAGGGGCGTAAGGGCTGGGTTTGGCCATACAAGGTTGGCT1360.10421615656944934No Hit
CTCGCTACGGGGGAACAGAGCAAACAAGCAGTAGAGATGAAGAATGATAAGT1360.10421615656944934No Hit
TAGTGTGGGGACCAGTTCTCTGCTGTGAGAGGCCGTTTCCATTCTTTCCTGC1350.10344986130055632No Hit
AATAGGGGTGGTGTAAAGTGAAGGTTGACTTTTGGATAAAGCCTCGTCCACA1350.10344986130055632No Hit
GATGTCGGGCATCCGCACTGTGGTGACAGGTTTGGGAAGCCAGTCTGGAGAG1350.10344986130055632No Hit
CTTTCAGGGTCCGATCTTAAGTGGGTGGACTGTCCTTGATAATCACAGGCAG1350.10344986130055632No Hit
CTAGACGGGTAAGGGAGAGCACACAAGAAGGTCTGTTGAAAATAACATGCCA1340.10268356603166331No Hit
TGACCAGGGAGACATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.10268356603166331No Hit
GGTTTCGGGGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCGTAGGCAGCCAT1330.1019172707627703No Hit
CATTAAGGGACCAGAATACCCACACTGGCTGAGGGCATGGGGTAAAGGTTAG1330.1019172707627703No Hit
ATCAGTGGGGAGCAAGAAAGTTTTGGAAATTGTGTTGCTTTTAAGAAATAGA1310.10038468022498429No Hit
TTTGGAGGGGAAGGACCCTGGCTACCTGGATTTCATCCCAGAGAAAAGGCTG1310.10038468022498429No Hit
AACGCTGGGATATTAGACCAGACCACCATGTGTCATTGTGTGTGTGTGTGTG1310.10038468022498429No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)