FastQCFastQC Report
Sat 31 Dec 2022
SRR5846270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129319
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGCCGTCT7090.5482566366891176RNA PCR Primer, Index 24 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACATCTCGTATGC6100.4717017607621463No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTATACAC1850.14305709137868372No Hit
TAGCTAGGGTACCGCACAGGCATGCTGCAGTGAATTTAACTGATCCTCTGTC1760.13609755720350453No Hit
ATGTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1700.13145786775338505No Hit
TCGCTAGGGAGCTTAGGTGATAAAATGAATGTACACCTTCCCACTTATTAAC1640.12681817830326556No Hit
TGCCTTGGGGGCACACATCAAAAGACAGCAAGGCAGCAAAGAAGAAGAAAAA1640.12681817830326556No Hit
GTTTAAGGGTGCTAGCCACATGTGGCTATTTAAATTTAGTTAAAATGGAATA1620.12527161515322574No Hit
TCTCAGGGGGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTA1600.12372505200318593No Hit
CTAATGGGCCCTAGAGAAGGATTTAGATCTGTTGGCCTCTGTTCCATCCCCT1600.12372505200318593No Hit
TGTTAGGGACCTCATCGGCATTGCTGCCATCGTGGTCGCTGTGATCATGATC1580.1221784888531461No Hit
CTAAATGGGAGGAGAACATTTGTAAGGGTAGAATATTTGTTTTCAGAGATGT1570.12140520727812619No Hit
ATCAGTGGGGAGCAAGAAAGTTTTGGAAATTGTGTTGCTTTTAAGAAATAGA1560.12063192570310627No Hit
AACACAGGAATGAACGTTTCTTCTTTAAGTACACCTTCCTCAGATACACCTC1550.11985864412808636No Hit
GGTTTCGGGGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCGTAGGCAGCCAT1540.11908536255306645No Hit
CAGCCAGGGTAATTTATAGATTCAAGGCCATCCCCTTAAGCTACCAATGACC1530.11831208097804653No Hit
TGAAATGGGATCAAAATCATAGCTGGCTCTTTTCCAGGAAATAAATAAGCTG1520.11753879940302663No Hit
CACATAGGGATTAAAAAGAAATGCTAAAATAAATCAGTTGTTGAGTCCTTGA1520.11753879940302663No Hit
ACGTCGGGTATTTATGCCACCAGTAATTTTATAAGAAATCTGAATAGTTCTC1520.11753879940302663No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTGTAACACTGTCTCTTA1490.11521895467796689No Hit
TACTATGGGAAGAAAATACTGTAAACAACTTTACATACATGATAGTTTAAGA1480.11444567310294697No Hit
TGTCACGGGGTTTGGGTCACTTCATTATGTTGACAAATTCCTTATTTGATAT1480.11444567310294697No Hit
TTACATGGGAGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCT1470.11367239152792706No Hit
CAAACGGGGTCAGCTTTACACTTCACAGGAGATTGATGGCATTGAGGAAGAT1470.11367239152792706No Hit
TTATGAGGGATGGCAAGAAAATACTGAATGCACTGCCATAATGTAAATATGA1460.11289910995290715No Hit
GCTGCTGGGCTTTGAGCCAGGGTGGGGAATGACTTTCTTTCCTGGAATACAC1450.11212582837788723No Hit
ACGTGGGGGATGACAGCTTTGTGGAGAAATGAAAACATTTGGAAAATAGTGT1440.11135254680286732No Hit
TCTAGCGGGAATCCCCATGGTAACTACAGAAAAATAACTAAAGAGTATACTC1430.11057926522784743No Hit
TCAACGGGGGGGATTCAAATTCAGTCCACAATGCAGTTGATTTGTATGTCTC1420.1098059836528275No Hit
AAAGTAGGGTCACTAATTTTCTCTTCAGCTATGTATATTCTGCTTTTTGTGA1410.1090327020778076No Hit
CGATTAGGGGAAGAAATGTGACTTTTGTAAGATTATTTCTCTTGTTATTGTC1410.1090327020778076No Hit
CGCGTGGGCGTACTGAAACATCGTCTTTACCCACTTCTCAGTTTATAATGGG1400.10825942050278767No Hit
GCTACTGGGAGTTACAGACATCCATACTCACTTTTTAAAATAACTTTTTAAA1400.10825942050278767No Hit
ATCTACGGGAATCTTTCTTTGCTGTGACTGAAATGTATCCCTCGTTTATCAT1380.10671285735274787No Hit
AATACCGGGGTCTGGAAAACCTGTGTGATCGGCACAAGTACAGCTGCCCACC1380.10671285735274787No Hit
CTTCAAGGGTATGTAATTAATTTCCTTTTAATATTTGTATTAATAAATTTAT1380.10671285735274787No Hit
AGTATGGGAGTCAGTGCTGGGCACACAGGAAGCTGCAGGGTATAGTTCCAGC1370.10593957577772793No Hit
CTCTTGGGGCATACTTGTCATTTCATAGCCATAAAATGTTCTATAGTGCTGA1370.10593957577772793No Hit
ACTTGGGAGGCTGAGGCAGGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTG1360.10516629420270804No Hit
TTGCCAGGGCTGAAAACCGAAAGTCAATTCTAATTATTCATCTTACATATTT1360.10516629420270804No Hit
TAGTTTGGGAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGAT1350.10439301262768813No Hit
GCTGCTGGGATTGCAGGCGTGAGCCACCACGCCCAGCCACACACATTTTTTT1350.10439301262768813No Hit
GATGCTGGCCACAACCTAGTTTTTTGTAACTAAATGTAGTAGATTTTTAAAT1350.10439301262768813No Hit
GTACTAGGGCCAACTCTGACTGTAAAATACATGGTATCTCTTTCTCCGTTGT1350.10439301262768813No Hit
TGGTGAGGGCAAAGCCCAAAGTCCCAGGCCAAGGGCAGACATTGTGAGCAGC1340.10361973105266821No Hit
TCCACTGGGGGTATTTGGTTAAAGGTCTCCCACAAGACTGGTATTCTCTTTG1340.10361973105266821No Hit
AAAGCAGGGAACTCTTTTGCAAAATGAGAACAATTAGATCATATTTTCATGG1330.1028464494776483No Hit
TGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG1330.1028464494776483No Hit
AATGCAGGGTGAATATTCTTGGTCATCTCGACTAATTCTGAGGCAATGATGG1320.10207316790262838No Hit
CAATAAGGGATTCATGAACAGACTTCATGATTTTACATGCTATCCAATGACA1320.10207316790262838No Hit
TAGTGTGGGGACCAGTTCTCTGCTGTGAGAGGCCGTTTCCATTCTTTCCTGC1310.10129988632760847No Hit
CACGCAAGGGACGTTGAGATTCCTCGGCGGGTAGGCAGCGGTGCTGGGCATG1310.10129988632760847No Hit
ACCAAAACAACATAGCAAACATACATATTTTGCATGCAAACCCTAAATATAT1310.10129988632760847No Hit
CTTAGGGGATTCACAGAATTGGCAGAGGGGGTCGATTTGGGAGGAAAGGTGT1300.10052660475258857No Hit
CCACTAGGGACAGTCACCAAGACATGCACTTTATAGGAAATTATAAGCTCCT1300.10052660475258857No Hit
GCACAGGGAATGCTTAAGTGTTCTGGTTATGCCATTCATTCATACGATGTGT1300.10052660475258857No Hit
TTCGTCGGGACAAGCAGTGAATGACTGCATTTGAAATATAAAACACAAAGTC1300.10052660475258857No Hit
CATTGCGGGTAACCCATGGGTTAAAGAAGAAATCAAAAGAAAAAATTACAAG1300.10052660475258857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)