FastQCFastQC Report
Sat 31 Dec 2022
SRR5846263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147171
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC11060.7515067506506037No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT8800.597943888401927No Hit
GTCTATGGGATGATTAATGACTATTAAACTGTAGGATTTCAGGCATTCGGCA2200.14948597210048176No Hit
CATCTCGGGAGCCAAATAGAGGGAACAAAGAAAATAATCTAAGAACTGAAGA2160.14676804533501844No Hit
GTAGCCGGGATGGGAAGAGGTTCCAGAATGTTGCCAAGGAAGGAGTGAAGTT2140.1454090819522868No Hit
AATAAAGGGAAACAAAACTTTGGACCAAAATCCCAGTTCAAAGAAACAAAAA2030.1379347833472627No Hit
TGGCCCGGGGATCTTGATGTGGGAACATTCCCCTTTTCATCTTTTCTGCGGG2030.1379347833472627No Hit
GATTGAGGGAGAAAAATCCGTCATCTTACCCTCTCACTTGCAGGAACATTAA1990.13521685658179938No Hit
GTGACAGGGGTTCTCTCTTCCTTGGCGACTGTTGAAAGCAGGGAAACATCCA1980.13453737489043358No Hit
ACTTCAGGGGGAAAATCTCAAGGGGATTAATTCCCATTCCTTCCTTTGAGGT1970.13385789319906777No Hit
ACATACGGGAATGCTTAACACTTATAGTCAGATTGCATTCTTTGTTTTCTTA1950.1324989298163361No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1950.1324989298163361No Hit
TAATTCGGGGGAACTTGAACAGGTCTGAGTATGTATTCCTACACACTGCTAA1920.13046048474223862No Hit
GATGTTGGGGTAATGTAGTGGTAGCAACCTGCATTTTGGATTCAAATCAAAG1900.12910152135950698No Hit
ACAAATGGGGTTGTGGTGAGGGATGGGAAACCTCAGCCTTCTGTCTTTGTAT1890.12842203966814114No Hit
GGCCTTGGGCAGAATTGAGTTTAGAATGATTTACTTGGGATGTGCGGCGATT1890.12842203966814114No Hit
TTTGTTGGGATTGGTGACAATTTTAACTGTGCTGTTTTCTGACTAGCAAATA1880.1277425579767753No Hit
TGTTCCTGGGGACATTACAGAGTGAAAACGTCTCTCAAGGTGGAATGCTTTA1880.1277425579767753No Hit
TGTTTAGGGACCAAAACCCTGATTTCTGGGTTTTTCAGTTTTTACATGGGAA1870.12706307628540947No Hit
CTTGCCGGGGGGGCAGGTGGTACAGAAAGAACGGAGTGGTTAGAGAGTCATT1860.12638359459404366No Hit
ACAGAGGGGGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGTTGGTATG1840.12502463121131202No Hit
CATTCTGGGAAGTGCCTGGATTTTACAACAGGAAGATACAGATCGACCTTCC1840.12502463121131202No Hit
TGTAGAGGGATGCGGCCGGGGAGAACCGCCTGGGAACAGACGAGTCCAAGTT1830.12434514951994617No Hit
CACAGCGGGATTGAGGTTTGAACTCATATGCAACCTTGGAACTGTCTATCAA1810.12298618613721453No Hit
GAACAAGGGTCCCTTAACAGCAGAATTGATCAAGCGGAATAAAGAATTAGTG1810.12298618613721453No Hit
AACACTGGGATACCCAAATTGAACCTTGCTGTTAAATTCATAGTTCATTTTG1800.12230670444584871No Hit
AAGTAAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1790.12162722275448289No Hit
GTCACTGGGCTGTCAATGGGACTCTTAGAATTGGACTTAGAAGCACGTGGGC1780.12094774106311706No Hit
AGTCCTTGGGGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAATAT1770.12026825937175123No Hit
CAGTTCGGGTTCAACTTAAAAGCCAGGAGATTCACAGTCTGCAGCTGAAAGC1750.11890929598901959No Hit
GGATCCGGGGAAATTGAGAGGCAAAAATAGGACAGAAAGGCAGAGAGAGAGG1750.11890929598901959No Hit
TTGTCAGGGGAAATGTAAAAGGAGACATTATAACTGATACTGCAGAAATTCA1740.11822981429765375No Hit
AAAATAGGGCTTTGGCTCATTGTCCAAATTATTAGAAATGCAATTTCAGCAG1740.11822981429765375No Hit
TGGCTTGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1730.11755033260628792No Hit
GGTACGGGGAACTTTGAAGAGAAAAACAAGTCATGGATTCATTAGTTAATGT1730.11755033260628792No Hit
ATTTGCGGGAAAGTAACAATACTTACTTTATAGGGTTGCTGTTATAATTAAA1720.1168708509149221No Hit
CCGTTAGGGATTTTCAGTAAGCTGAGATAATTGGAATTTAAGGTAAGACTTT1720.1168708509149221No Hit
GTTAAAGGTATTTGACTCTGTGGCTCTAAGACTGCCTTGAACCGCCTGAGGC1710.11619136922355627No Hit
GGTTGTGGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAG1710.11619136922355627No Hit
TTCAAGGGGCTTGATAAACATAGATGGTAAAATCCTTAACAAAATACTAGCT1700.11551188753219045No Hit
TCCTGAGGGATAGAATATTGAATAAATAAATAGAAATATAGAAGGTGTTTTT1680.11415292414945878No Hit
ATTTTAGGGATTTGCAACTTGTTGAGGAAACAGAAGAGTAGATTGTAACCAT1680.11415292414945878No Hit
CTGCTTCTGGGCAGCTACACTAGCCTCTAACAAGAGTCAGCCTGTTCTTTGA1670.11347344245809297No Hit
TATCAGGGGTTCTGATCTCTCTTTCTGGAGATCCTATTCTTCGAATGTTGGA1660.11279396076672714No Hit
ATGGTGGGGGGATACAAACAAGTGGAAGAACATTCCGTGCTCATGGGTAGGA1650.11211447907536132No Hit
TCGTCAGGGGACCATAGGTTTGTGTTGTGGATGGCAATTCTAGAATAGGGCT1650.11211447907536132No Hit
TCAGCTGGGTGAATATCCACATGCAAAAGATAAAGTTTGACATTTATCTTAC1650.11211447907536132No Hit
GATTACGGGTGAGGTCAATGATCCAGGGGAACCCTCAGGGCTGGAGACTCTG1650.11211447907536132No Hit
ATAGATGGGGAAAGATGAAGATGATGATCTTGTGTCACCCCCTAACACAGAA1640.11143499738399548No Hit
ATCCGGGATTCTTTAAAGTGAAGGCTTAGTTCAAATTATTATTAAAATTGCT1640.11143499738399548No Hit
CTGCAGGGGGGCTGGGCTTGACATTCCTCCTGCCAGTCCCTGGGTGCCCTAC1640.11143499738399548No Hit
TTCCGTGGGCATGATGACTCAAATTGGTTTTGCAGTTCATTTTGAAATAAGA1630.11075551569262967No Hit
ATAGCTGGGGACGACATTGTGAAACCCTGCCAAAACACACACACACACACAC1620.11007603400126384No Hit
TCGCACGGGTCCTTGGCACCTAAAAGATAATTCTCCTCTGACGCAGGTTGGA1610.109396552309898No Hit
TCTCAGGGAGCTATGGCCAATAGGCTATAAAGAGACATTTAGCACTTTTTTC1610.109396552309898No Hit
GTTCACGGGAAGTACAAGTGATTGTGACAAATGACGTAAAAATGGCATTCAT1610.109396552309898No Hit
ACTAGTGGGACGCTGTGTCCCGCAATGTTTTAATTATAGGGTTCATCTGCTG1610.109396552309898No Hit
GACTAAGGGGGCTTGGAAGGTCCATGTTAAATATTGTATTTGGTATAACACC1600.10871707061853217No Hit
AATCATGGGCTTCCCAATGTAGGTGGACATTGTGTAATATGTTGAGGACCTC1590.10803758892716636No Hit
TCTGCAGGGATGGTGAGCAATTTTAAGAAATGTTAGTGAAAGGTAGAAATTA1580.10735810723580053No Hit
AGCTAAGGGTGGCAGAAGCAGCAGGAAAGACAAAAGAGGGTGTTCTCTATGT1580.10735810723580053No Hit
CACACGGGGGATAGAATTTTAAAATCTAATTCTGACTTGTATGGTTAAGAAA1580.10735810723580053No Hit
TTGGTTGGGGAGTGAGCAGGCGGGACCTGGATATTAACGTCTTTCCTAAAAG1570.1066786255444347No Hit
AATATAGGGAGAGATCAAAAGCTCTTTAAGACAATTTGATATCATGGATAGA1560.10599914385306887No Hit
ACGACTGGGGATCTTAGCTGGATCTTCTGGGTAACTTGCTGCAGCTTCTACA1550.10531966216170306No Hit
ACTTATGGGATTTATACAAGTAAAGGGCCGGACCTCTTGCTTCTTAAGTTAT1550.10531966216170306No Hit
CATGACGGGCTACTGGTAAGGAAACCAAGTGTCCTCTGTGCCTTTTTTCTTT1550.10531966216170306No Hit
TCTCTGGGATGCCTGTGACTTTTAAAGATGAAAAGGAAAGTTCTCTATCTCT1540.10464018047033723No Hit
AGGATTGGGCTTGGAAAGTGATTTCATTCTCTGGTGCTTTAAAACAGATTTC1540.10464018047033723No Hit
GGGCTGGGAGAAAGCAAGTAGGGCCATCCAGCTGCCAGAGTGCTCCACAGGG1540.10464018047033723No Hit
CCTCCAGGGCAGGCATGTCCAGTGAGAAATGCTGAGAGACTCTTTCTCTTCT1530.10396069877897139No Hit
CAGCCAGGGAGCACAATGTCTTTCTATAAGATATTTTTAATGATTTAGTATT1530.10396069877897139No Hit
TCTAAAGGGCATTTAGTTTCTTTCCTTAGAACAGACATAAATTTAATTTCAT1530.10396069877897139No Hit
TCCATTGGGCTGATTTCTTGGAGCAGGAATTTTAGAGATTGAAATGAATGAT1530.10396069877897139No Hit
TCATGCGGGCATTTAAAATAAATAGTTTTTAATTGTAGGCTCAACATCTGTT1530.10396069877897139No Hit
GCAGGTGGGGCTCTTCCGATCTACGTGTGGGCAATTGTAAACGATTTTTGTA1530.10396069877897139No Hit
TTGCTGGGGCTAGTAGCTCTCCCATCTGCCACTGCCTGCCTCTGAGATTCAC1520.10328121708760558No Hit
CATTAAGGGCAGCCATTTGAGAAAGAAGGAAAAGAAAAAAAATCAAAATAAG1520.10328121708760558No Hit
GCAAGAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGA1520.10328121708760558No Hit
GGAATTGGGACACAGAAACCTTTTTTGAAAATTAAAAACATTAAGAAACTTC1520.10328121708760558No Hit
CTAATAGGGCATTCATTTTGCTCAGGAAACTTTATTGGTTTTGTCTGTGTAA1510.10260173539623975No Hit
TCTGATGGGGTCCCTTCATTTCAGGGGAGGTTTGATAGCTACTTGCTACGCT1510.10260173539623975No Hit
ACTGCTGGGGGATAAGTCAAATGTGACATTTCATTGAGACCTGCTTCCTTAC1510.10260173539623975No Hit
TGACCGGGGGATTAGGTGGGGCGCCAGAGCCGGGGCACCTGCGCAGGCTTGG1500.10192225370487393No Hit
ACACAAGGGCGATCTGGTATGGGTGGGGAAAATATCTTTCATATTTGTTTGC1490.10124277201350809No Hit
ATGTAGGGATCTTCAAGGCATTCTGAAAAGATAGCTATTAGAGATTTTCAGG1490.10124277201350809No Hit
ATATAACGGGGAAGACTGAATGAGATAAATGAAAAGCTGTTTGCAGTATGGT1490.10124277201350809No Hit
AACAAGGGAATAAACATGGCAGCAAGTAAACAGTGTGATTGTGAAAAAAATA1490.10124277201350809No Hit
ACAGTAGGGGGAATTGAGAATTTATTTGAAATAGAATAGGTAACACCTCAGC1490.10124277201350809No Hit
CTAATAGGGTCCCCTTTGAGTGCATTTAACTCTTTAGTATCTTCTCTCTTAC1490.10124277201350809No Hit
GCACTCCAGCCTGGGCAACAGAGACAGACTCCGTCTCAAAACAAACAAACAA1480.10056329032214228No Hit
TTGCGTGGGGACAGGCAAAAAAATACATACAAAAGATCTATAAAACCAAAAG1480.10056329032214228No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)