FastQCFastQC Report
Sat 31 Dec 2022
SRR5846262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146707
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC10280.7007163939007682No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT8330.5677984008942996No Hit
GTAGCCGGGATGGGAAGAGGTTCCAGAATGTTGCCAAGGAAGGAGTGAAGTT2190.14927713060726483No Hit
TGTTTAGGGACCAAAACCCTGATTTCTGGGTTTTTCAGTTTTTACATGGGAA2160.14723223840716532No Hit
ACTTCAGGGGGAAAATCTCAAGGGGATTAATTCCCATTCCTTCCTTTGAGGT2130.14518734620706578No Hit
ACAAATGGGGTTGTGGTGAGGGATGGGAAACCTCAGCCTTCTGTCTTTGTAT2120.14450571547369928No Hit
ACATACGGGAATGCTTAACACTTATAGTCAGATTGCATTCTTTGTTTTCTTA2040.13905266960676724No Hit
AATAAAGGGAAACAAAACTTTGGACCAAAATCCCAGTTCAAAGAAACAAAAA2040.13905266960676724No Hit
GATGTTGGGGTAATGTAGTGGTAGCAACCTGCATTTTGGATTCAAATCAAAG2010.13700777740666772No Hit
TCAGCTGGGTGAATATCCACATGCAAAAGATAAAGTTTGACATTTATCTTAC1970.1342812544732017No Hit
AAGTAAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1960.13359962373983517No Hit
CTGCTTCTGGGCAGCTACACTAGCCTCTAACAAGAGTCAGCCTGTTCTTTGA1950.13291799300646867No Hit
CATTCTGGGAAGTGCCTGGATTTTACAACAGGAAGATACAGATCGACCTTCC1920.13087310080636916No Hit
TCTCTGGGATGCCTGTGACTTTTAAAGATGAAAAGGAAAGTTCTCTATCTCT1910.13019147007300264No Hit
GTGACAGGGGTTCTCTCTTCCTTGGCGACTGTTGAAAGCAGGGAAACATCCA1910.13019147007300264No Hit
AGAAAAGGGATGAGAAAGCTGATTTTTAAAAAAGTTTTCTTTATTTCTGATA1900.12950983933963614No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1890.12882820860626965No Hit
CATCTCGGGAGCCAAATAGAGGGAACAAAGAAAATAATCTAAGAACTGAAGA1870.12746494713953663No Hit
AACACTGGGATACCCAAATTGAACCTTGCTGTTAAATTCATAGTTCATTTTG1870.12746494713953663No Hit
AATCATGGGCTTCCCAATGTAGGTGGACATTGTGTAATATGTTGAGGACCTC1860.12678331640617013No Hit
TTCAAGGGGCTTGATAAACATAGATGGTAAAATCCTTAACAAAATACTAGCT1860.12678331640617013No Hit
TAATTCGGGGGAACTTGAACAGGTCTGAGTATGTATTCCTACACACTGCTAA1830.12473842420607059No Hit
CTTGCCGGGGGGGCAGGTGGTACAGAAAGAACGGAGTGGTTAGAGAGTCATT1820.1240567934727041No Hit
TTGTCAGGGGAAATGTAAAAGGAGACATTATAACTGATACTGCAGAAATTCA1810.12337516273933759No Hit
GTCTATGGGATGATTAATGACTATTAAACTGTAGGATTTCAGGCATTCGGCA1800.12269353200597109No Hit
GGTACGGGGAACTTTGAAGAGAAAAACAAGTCATGGATTCATTAGTTAATGT1800.12269353200597109No Hit
GGTTTGGGGTCCTTGATTTGGTTTTTAAATTTTGCTTTTGTGTGTGTGTCTT1790.12201190127260458No Hit
GGATCCGGGGAAATTGAGAGGCAAAAATAGGACAGAAAGGCAGAGAGAGAGG1780.12133027053923806No Hit
GGTTGTGGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAG1780.12133027053923806No Hit
TCTCAGGGAGCTATGGCCAATAGGCTATAAAGAGACATTTAGCACTTTTTTC1770.12064863980587157No Hit
CAGCCAGGGAGCACAATGTCTTTCTATAAGATATTTTTAATGATTTAGTATT1750.11928537833913856No Hit
TGGCCCGGGGATCTTGATGTGGGAACATTCCCCTTTTCATCTTTTCTGCGGG1750.11928537833913856No Hit
ACGACTGGGGATCTTAGCTGGATCTTCTGGGTAACTTGCTGCAGCTTCTACA1740.11860374760577204No Hit
ATTTGCGGGAAAGTAACAATACTTACTTTATAGGGTTGCTGTTATAATTAAA1740.11860374760577204No Hit
TTGCTGGGGCTAGTAGCTCTCCCATCTGCCACTGCCTGCCTCTGAGATTCAC1730.11792211687240554No Hit
GCAAGAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGA1720.11724048613903904No Hit
TGAGCGGGGCAGAATGGAGTCTACTGTTACTCACTTGAATATAATTCTCTTT1720.11724048613903904No Hit
TGGCTTGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1710.11655885540567254No Hit
ATCCGGGATTCTTTAAAGTGAAGGCTTAGTTCAAATTATTATTAAAATTGCT1710.11655885540567254No Hit
CAGTTCGGGTTCAACTTAAAAGCCAGGAGATTCACAGTCTGCAGCTGAAAGC1700.11587722467230603No Hit
CACACGGGGGATAGAATTTTAAAATCTAATTCTGACTTGTATGGTTAAGAAA1700.11587722467230603No Hit
GAACAAGGGTCCCTTAACAGCAGAATTGATCAAGCGGAATAAAGAATTAGTG1690.11519559393893951No Hit
AAATAAGGGACTGGGTATATATTCAGAGGAGAGGAGGTCAGTGTGTTAGGGA1680.11451396320557301No Hit
AGTCCTTGGGGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAATAT1680.11451396320557301No Hit
AGGATTGGGCTTGGAAAGTGATTTCATTCTCTGGTGCTTTAAAACAGATTTC1680.11451396320557301No Hit
GCAGGTGGGGCTCTTCCGATCTACGTGTGGGCAATTGTAAACGATTTTTGTA1680.11451396320557301No Hit
GGCCTTGGGCAGAATTGAGTTTAGAATGATTTACTTGGGATGTGCGGCGATT1670.1138323324722065No Hit
ATTTTAGGGATTTGCAACTTGTTGAGGAAACAGAAGAGTAGATTGTAACCAT1660.11315070173884001No Hit
TAACAGGGATTGCAGAGGTTCAGAAAAGAGACCCTCGTGACTGGACTGCACA1650.11246907100547349No Hit
AAGCACGGGACTGCAGGGAGGAGGAAATGGGGGGTGGGGTTATTGTTTAACG1640.11178744027210699No Hit
ACAGAGGGGGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGTTGGTATG1640.11178744027210699No Hit
GGAATTGGGACACAGAAACCTTTTTTGAAAATTAAAAACATTAAGAAACTTC1640.11178744027210699No Hit
GATTGAGGGAGAAAAATCCGTCATCTTACCCTCTCACTTGCAGGAACATTAA1610.10974254807200746No Hit
TATCCCCTGGACACCATTAGATGAAAGGAATATTAGGAATGTGAATTACTAG1610.10974254807200746No Hit
ATACGCGGGAATGTTTGCCATTTTTGCATTGTTTCGTTTTTAACTGGAACAT1610.10974254807200746No Hit
ACTAGTGGGACGCTGTGTCCCGCAATGTTTTAATTATAGGGTTCATCTGCTG1610.10974254807200746No Hit
ATATATGGGAAGTGTCCAATCTCCTGATTAGAATAGCTTTTTGGTTCATCTC1600.10906091733864096No Hit
AGCAATGGGTACAGATCTCATGGACATTAAAAGGATAATCAAGGGATATTAT1600.10906091733864096No Hit
TGTATTGGGAGATAATCCCAGGTTTCTCTAGCTCCTTCCATGCTGCCATTTC1600.10906091733864096No Hit
TTTGTTGGGATTGGTGACAATTTTAACTGTGCTGTTTTCTGACTAGCAAATA1590.10837928660527446No Hit
GTTCACGGGAAGTACAAGTGATTGTGACAAATGACGTAAAAATGGCATTCAT1590.10837928660527446No Hit
TTAGCGTGGGAATGATAATAAAAGTAAACAAATCAGCATCTTTTTCCCCTTC1590.10837928660527446No Hit
GCTTTTGGGACCTACACTCCCGGTTGCTGGAGAGAGCAGCCAAAATGAACGA1580.10769765587190795No Hit
TGCAGCGGGAATGCATTTTCTAATATAATTACATAGACTCTTTCAGGACCAG1580.10769765587190795No Hit
TTGTAAGGGGGGTAAAGCTTTTGTTTAAGAACCCATAGCTGCATGTTTCCAG1570.10701602513854146No Hit
GTTAAAGGTATTTGACTCTGTGGCTCTAAGACTGCCTTGAACCGCCTGAGGC1560.10633439440517493No Hit
CCTCCAGGGCAGGCATGTCCAGTGAGAAATGCTGAGAGACTCTTTCTCTTCT1560.10633439440517493No Hit
CACAGCGGGATTGAGGTTTGAACTCATATGCAACCTTGGAACTGTCTATCAA1560.10633439440517493No Hit
TATCAGGGGTTCTGATCTCTCTTTCTGGAGATCCTATTCTTCGAATGTTGGA1560.10633439440517493No Hit
GACTAAGGGGGCTTGGAAGGTCCATGTTAAATATTGTATTTGGTATAACACC1560.10633439440517493No Hit
TCTAAAGGGCATTTAGTTTCTTTCCTTAGAACAGACATAAATTTAATTTCAT1560.10633439440517493No Hit
TTGCGTGGGGACAGGCAAAAAAATACATACAAAAGATCTATAAAACCAAAAG1550.10565276367180843No Hit
GATTACGGGTGAGGTCAATGATCCAGGGGAACCCTCAGGGCTGGAGACTCTG1540.10497113293844193No Hit
AGGTATGGGCTACTGAATTTGTTCAGATTTTGGCCTCTTTTCAGAAATCTGG1540.10497113293844193No Hit
TCGCACGGGTCCTTGGCACCTAAAAGATAATTCTCCTCTGACGCAGGTTGGA1530.10428950220507544No Hit
AAAGAAGGGGTAGATACCAGGAAGAGACTGAGCACAATTGATGAGCTGGATG1530.10428950220507544No Hit
ATAGATGGGGAAAGATGAAGATGATGATCTTGTGTCACCCCCTAACACAGAA1530.10428950220507544No Hit
TCCTGAGGGATAGAATATTGAATAAATAAATAGAAATATAGAAGGTGTTTTT1520.10360787147170891No Hit
TCTGCAGGGATGGTGAGCAATTTTAAGAAATGTTAGTGAAAGGTAGAAATTA1520.10360787147170891No Hit
GTAGGTGGGGTAGTGAAGACTTTCTAAAATAGCTATTTTTAAGAATGTATTG1510.1029262407383424No Hit
TTGCCAGGGAGTCTTGAAAAATCTACCTGAGGGAATATTTTAGAATAATTTC1510.1029262407383424No Hit
TCATGCGGGCATTTAAAATAAATAGTTTTTAATTGTAGGCTCAACATCTGTT1510.1029262407383424No Hit
GTACATGGGCAATCATAAGGATATGAACTCTTTAAATACTAAAATGAATACT1500.10224461000497591No Hit
GCACTCCAGCCTGGGCAACAGAGACAGACTCCGTCTCAAAACAAACAAACAA1500.10224461000497591No Hit
CGAAGTGGGTCAGCTGAGCTGTGTAGTTCATATGAATTAATTAACCCCTTAC1500.10224461000497591No Hit
TGCCGAGGGGGAGGTATGTTCTTTCAGTGTCTAGCTTCTTGAGGGTTTTTAT1500.10224461000497591No Hit
GTATAAGGGAACCTTTTAGAACCGGAATTGAAACAATATTTATAAAAAAAAG1500.10224461000497591No Hit
TGCTCTGGGATTTCATATTTGTAGTTATGTAGTCTGGTCAAAAATAAGAGAA1490.1015629792716094No Hit
ATGGTGGGGGGATACAAACAAGTGGAAGAACATTCCGTGCTCATGGGTAGGA1490.1015629792716094No Hit
TCTGATGGGGTCCCTTCATTTCAGGGGAGGTTTGATAGCTACTTGCTACGCT1490.1015629792716094No Hit
CCAATTGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGGC1490.1015629792716094No Hit
CCGTTAGGGATTTTCAGTAAGCTGAGATAATTGGAATTTAAGGTAAGACTTT1490.1015629792716094No Hit
TGTAGAGGGATGCGGCCGGGGAGAACCGCCTGGGAACAGACGAGTCCAAGTT1480.1008813485382429No Hit
ATAGAGGGATTTGAAATAATATACAAGAATATAGTGTGCAAAATTTAGGGGC1480.1008813485382429No Hit
ATGCGAGGGTGGCACAGACTTTTTGGAATATAAATGTCGCTACTGCTGTTCA1480.1008813485382429No Hit
GCGCTTGGGATAGCTGCATATAAATCTTCATAATTTGTAAGGGATATTTCTC1480.1008813485382429No Hit
TATAATGGGTCAGGCATGATAGATGTTTTTTACATCATGGGGAATATGCCAA1470.10019971780487638No Hit
TTGTACGGGATCTGTTGATACTTACTGTAATAGAAATTGAAACTAAGAAGTT1470.10019971780487638No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)