FastQCFastQC Report
Sat 31 Dec 2022
SRR5846261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146712
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC11110.7572659359834233No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT8680.5916353127215225No Hit
GTAGCCGGGATGGGAAGAGGTTCCAGAATGTTGCCAAGGAAGGAGTGAAGTT2120.14450079066470364No Hit
GTCTATGGGATGATTAATGACTATTAAACTGTAGGATTTCAGGCATTCGGCA2100.14313757565843285No Hit
TTTGTTGGGATTGGTGACAATTTTAACTGTGCTGTTTTCTGACTAGCAAATA2060.14041114564589127No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1990.1356398931239435No Hit
ACTTCAGGGGGAAAATCTCAAGGGGATTAATTCCCATTCCTTCCTTTGAGGT1980.1349582856208081No Hit
CTTGCCGGGGGGGCAGGTGGTACAGAAAGAACGGAGTGGTTAGAGAGTCATT1980.1349582856208081No Hit
ATCCGGGATTCTTTAAAGTGAAGGCTTAGTTCAAATTATTATTAAAATTGCT1920.13086864060199577No Hit
GTGACAGGGGTTCTCTCTTCCTTGGCGACTGTTGAAAGCAGGGAAACATCCA1920.13086864060199577No Hit
TGTTTAGGGACCAAAACCCTGATTTCTGGGTTTTTCAGTTTTTACATGGGAA1910.13018703309886037No Hit
AACAAGGGAATAAACATGGCAGCAAGTAAACAGTGTGATTGTGAAAAAAATA1890.12882381809258958No Hit
CATTCTGGGAAGTGCCTGGATTTTACAACAGGAAGATACAGATCGACCTTCC1870.1274606030863188No Hit
TGGCCCGGGGATCTTGATGTGGGAACATTCCCCTTTTCATCTTTTCTGCGGG1860.1267789955831834No Hit
ACAAATGGGGTTGTGGTGAGGGATGGGAAACCTCAGCCTTCTGTCTTTGTAT1840.1254157805769126No Hit
ACAGAGGGGGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGTTGGTATG1830.12473417307377721No Hit
TCGCACGGGTCCTTGGCACCTAAAAGATAATTCTCCTCTGACGCAGGTTGGA1820.12405256557064182No Hit
ATATAACGGGGAAGACTGAATGAGATAAATGAAAAGCTGTTTGCAGTATGGT1820.12405256557064182No Hit
CCAATTGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGGC1820.12405256557064182No Hit
ACATACGGGAATGCTTAACACTTATAGTCAGATTGCATTCTTTGTTTTCTTA1810.12337095806750642No Hit
AATAAAGGGAAACAAAACTTTGGACCAAAATCCCAGTTCAAAGAAACAAAAA1810.12337095806750642No Hit
TAATCTGGGGCCTGAGACTTGCCATAAGAAACTGCTGAGTACTTGGCAAACC1800.12268935056437102No Hit
CAGCCAGGGAGCACAATGTCTTTCTATAAGATATTTTTAATGATTTAGTATT1790.12200774306123563No Hit
GAACAAGGGTCCCTTAACAGCAGAATTGATCAAGCGGAATAAAGAATTAGTG1780.12132613555810023No Hit
CATCTCGGGAGCCAAATAGAGGGAACAAAGAAAATAATCTAAGAACTGAAGA1780.12132613555810023No Hit
ATTTGCGGGAAAGTAACAATACTTACTTTATAGGGTTGCTGTTATAATTAAA1780.12132613555810023No Hit
GGATCCGGGGAAATTGAGAGGCAAAAATAGGACAGAAAGGCAGAGAGAGAGG1760.11996292055182943No Hit
ATAGATGGGGAAAGATGAAGATGATGATCTTGTGTCACCCCCTAACACAGAA1760.11996292055182943No Hit
CACAGCGGGATTGAGGTTTGAACTCATATGCAACCTTGGAACTGTCTATCAA1750.11928131304869403No Hit
TCTCAGGGAGCTATGGCCAATAGGCTATAAAGAGACATTTAGCACTTTTTTC1740.11859970554555864No Hit
TTGCTGGGGCTAGTAGCTCTCCCATCTGCCACTGCCTGCCTCTGAGATTCAC1730.11791809804242324No Hit
AGTCCTTGGGGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAATAT1730.11791809804242324No Hit
TTGTCAGGGGAAATGTAAAAGGAGACATTATAACTGATACTGCAGAAATTCA1720.11723649053928785No Hit
AAGTAAGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA1720.11723649053928785No Hit
TGAGCGGGGCAGAATGGAGTCTACTGTTACTCACTTGAATATAATTCTCTTT1720.11723649053928785No Hit
GATGTTGGGGTAATGTAGTGGTAGCAACCTGCATTTTGGATTCAAATCAAAG1710.11655488303615245No Hit
AAAATAGGGCTTTGGCTCATTGTCCAAATTATTAGAAATGCAATTTCAGCAG1710.11655488303615245No Hit
AGGATTGGGCTTGGAAAGTGATTTCATTCTCTGGTGCTTTAAAACAGATTTC1710.11655488303615245No Hit
TCAGCTGGGTGAATATCCACATGCAAAAGATAAAGTTTGACATTTATCTTAC1700.11587327553301706No Hit
TCTCTGGGATGCCTGTGACTTTTAAAGATGAAAAGGAAAGTTCTCTATCTCT1680.11451006052674627No Hit
AGCTAAGGGTGGCAGAAGCAGCAGGAAAGACAAAAGAGGGTGTTCTCTATGT1680.11451006052674627No Hit
AATCATGGGCTTCCCAATGTAGGTGGACATTGTGTAATATGTTGAGGACCTC1670.11382845302361087No Hit
GGTACGGGGAACTTTGAAGAGAAAAACAAGTCATGGATTCATTAGTTAATGT1660.11314684552047548No Hit
CACACGGGGGATAGAATTTTAAAATCTAATTCTGACTTGTATGGTTAAGAAA1660.11314684552047548No Hit
GTTCACGGGAAGTACAAGTGATTGTGACAAATGACGTAAAAATGGCATTCAT1650.11246523801734008No Hit
GATTGAGGGAGAAAAATCCGTCATCTTACCCTCTCACTTGCAGGAACATTAA1650.11246523801734008No Hit
TGAACGGGCAGAAGTAAAAGTTTTTCCTCTCATCTTCTGACCTTCAGCCTCT1650.11246523801734008No Hit
TAATCCGGGCCATTCCTAGCCATAAGTTCTCAATTCTTAAGAGACATGGCAA1640.11178363051420469No Hit
TGCCGAGGGGGAGGTATGTTCTTTCAGTGTCTAGCTTCTTGAGGGTTTTTAT1640.11178363051420469No Hit
AATTATGGGGGAAAAGCACAATCTACATTTAGTGAATATTTCATAGAATATT1640.11178363051420469No Hit
TATCAGGGGTTCTGATCTCTCTTTCTGGAGATCCTATTCTTCGAATGTTGGA1620.1104204155079339No Hit
TCCCAGGGGCTAAGAATGGTTGTTACATTTTCAGATAGTTGGGGAAAAAAAA1610.10973880800479852No Hit
AACATAGGGATCTTAAAATTTCCAACACCTTTTGTGCAGTGATCACAAAGTC1610.10973880800479852No Hit
GCAAGAGGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGA1610.10973880800479852No Hit
AGCAATGGGTACAGATCTCATGGACATTAAAAGGATAATCAAGGGATATTAT1610.10973880800479852No Hit
ACTAGTGGGACGCTGTGTCCCGCAATGTTTTAATTATAGGGTTCATCTGCTG1610.10973880800479852No Hit
TCATGCGGGCATTTAAAATAAATAGTTTTTAATTGTAGGCTCAACATCTGTT1610.10973880800479852No Hit
GCTATGGGGATAATTGAGATATTTATAACCTTAAATATTTTTCTTTTGTCTA1600.10905720050166312No Hit
CAGTTCGGGTTCAACTTAAAAGCCAGGAGATTCACAGTCTGCAGCTGAAAGC1590.10837559299852773No Hit
AAATAAGGGACTGGGTATATATTCAGAGGAGAGGAGGTCAGTGTGTTAGGGA1590.10837559299852773No Hit
ATTAATGGGCTATTCCTTTCTTAAACCTACCAGCAAACTAGGATTGTGATAG1590.10837559299852773No Hit
CCGTTAGGGATTTTCAGTAAGCTGAGATAATTGGAATTTAAGGTAAGACTTT1590.10837559299852773No Hit
CTGCTTCTGGGCAGCTACACTAGCCTCTAACAAGAGTCAGCCTGTTCTTTGA1580.10769398549539233No Hit
GGCCTTGGGCAGAATTGAGTTTAGAATGATTTACTTGGGATGTGCGGCGATT1580.10769398549539233No Hit
TCAGTTGGGCAAGAGAAGAGTTGGACTTCCAGGCTGAAGGGGAGAAAGTGAC1570.10701237799225694No Hit
TTCAAGGGGCTTGATAAACATAGATGGTAAAATCCTTAACAAAATACTAGCT1570.10701237799225694No Hit
ACGACTGGGGATCTTAGCTGGATCTTCTGGGTAACTTGCTGCAGCTTCTACA1560.10633077048912154No Hit
CTCGCTGGGGTGTCTTTAATTTCCTCTTCAATATGACAACTAACAAAAGCCA1560.10633077048912154No Hit
GTTAAAGGTATTTGACTCTGTGGCTCTAAGACTGCCTTGAACCGCCTGAGGC1550.10564916298598614No Hit
GGTTGTGGGGAGACAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAG1550.10564916298598614No Hit
GCCCGCGGGGACTGAAAGTTAACAAGGATATCCAGGAATTGAACTCAGCTCT1550.10564916298598614No Hit
GTATAAGGGAACCTTTTAGAACCGGAATTGAAACAATATTTATAAAAAAAAG1550.10564916298598614No Hit
TGGCTTGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1540.10496755548285075No Hit
GCTTTTGGGACCTACACTCCCGGTTGCTGGAGAGAGCAGCCAAAATGAACGA1540.10496755548285075No Hit
GTCACTGGGCTGTCAATGGGACTCTTAGAATTGGACTTAGAAGCACGTGGGC1540.10496755548285075No Hit
TAATTCGGGGGAACTTGAACAGGTCTGAGTATGTATTCCTACACACTGCTAA1540.10496755548285075No Hit
GCAGGTGGGGCTCTTCCGATCTACGTGTGGGCAATTGTAAACGATTTTTGTA1540.10496755548285075No Hit
GGAATTGGGACACAGAAACCTTTTTTGAAAATTAAAAACATTAAGAAACTTC1540.10496755548285075No Hit
TAATGGCGGGACAGTCCAAAAATGGTTATTGAGCAATGGCCACATATTGGAA1530.10428594797971535No Hit
TTGCCAGGGAGTCTTGAAAAATCTACCTGAGGGAATATTTTAGAATAATTTC1530.10428594797971535No Hit
TGTTCCTGGGGACATTACAGAGTGAAAACGTCTCTCAAGGTGGAATGCTTTA1530.10428594797971535No Hit
TGACCGGGGGATTAGGTGGGGCGCCAGAGCCGGGGCACCTGCGCAGGCTTGG1530.10428594797971535No Hit
TATCCCCTGGACACCATTAGATGAAAGGAATATTAGGAATGTGAATTACTAG1530.10428594797971535No Hit
AAGCATGGGGATAGAGCAGAGTGGACCACCCCATGAACCTCGGTAAGAGACC1530.10428594797971535No Hit
TCTGCAGGGATGGTGAGCAATTTTAAGAAATGTTAGTGAAAGGTAGAAATTA1520.10360434047657996No Hit
AATTATGGGTTATCGGGTTATGGTGAGTATTGAATAAGTTGATGTAAATTAC1520.10360434047657996No Hit
ACAATAGGGGTAATGAGAAAATGTAATTATTTTATGTGTAATTGGAGTTGGT1520.10360434047657996No Hit
TGTAGAGGGATGCGGCCGGGGAGAACCGCCTGGGAACAGACGAGTCCAAGTT1520.10360434047657996No Hit
CATGACGGGCTACTGGTAAGGAAACCAAGTGTCCTCTGTGCCTTTTTTCTTT1520.10360434047657996No Hit
TTCCGTGGGCATGATGACTCAAATTGGTTTTGCAGTTCATTTTGAAATAAGA1510.10292273297344456No Hit
CCTCCAGGGCAGGCATGTCCAGTGAGAAATGCTGAGAGACTCTTTCTCTTCT1510.10292273297344456No Hit
TATTGGGGGACTTTAAACAATGAAACTTTTTCAAAAAGAAAAAACAAAAACT1510.10292273297344456No Hit
TTACGCGGGATTCTAATATTTAATATTTTTCTATTTTTGAAATAGGAAATGG1510.10292273297344456No Hit
ACATAAGGGTAGCTTTATTTGAAAACTCCAAAAGCTACTTAATCTTACCAGA1500.10224112547030917No Hit
CTAAAGGGGAGAGGTTGCAGTGAGCTGAGATCTTGCCACTGCACACCAGCCT1500.10224112547030917No Hit
CTAATAGGGCATTCATTTTGCTCAGGAAACTTTATTGGTTTTGTCTGTGTAA1490.10155951796717377No Hit
ATAGCTGGGGACGACATTGTGAAACCCTGCCAAAACACACACACACACACAC1490.10155951796717377No Hit
GATTACGGGTGAGGTCAATGATCCAGGGGAACCCTCAGGGCTGGAGACTCTG1490.10155951796717377No Hit
GTGACAGGGCAAGACTCATCAAAAAAACAAACAAACAAAAAGATAAATAAAA1490.10155951796717377No Hit
TTTGGGGGTCACTGAAGGAATTGCATGATGCATCAGACTTGATGGCAAATGA1480.10087791046403838No Hit
AGGTATGGGCTACTGAATTTGTTCAGATTTTGGCCTCTTTTCAGAAATCTGG1480.10087791046403838No Hit
TAGAACGGGAAATCAAAAATTGGATAATTTGTAATATTTATGTGTTAATCAC1480.10087791046403838No Hit
TATCCAGGGACTGACAGTGTATGGGGTCCATCTATTCTGCAACAAAGTCAGC1480.10087791046403838No Hit
ACAGCTAGGGATGATGTTGAAGTAAAAGAGTTGGTTATATTTTCACCTTTGG1470.10019630296090298No Hit
GCTACAGGGATACAGGAATTTTCCTAACAGCCTCTGATTCATTGCTAGTTAT1470.10019630296090298No Hit
GTAGGTGGGGTAGTGAAGACTTTCTAAAATAGCTATTTTTAAGAATGTATTG1470.10019630296090298No Hit
ACACAAGGGGACCATACAAGCATCCGAACTATTGAAGAATTGGCTGGAAAAA1470.10019630296090298No Hit
ATGCGAGGGTGGCACAGACTTTTTGGAATATAAATGTCGCTACTGCTGTTCA1470.10019630296090298No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)