FastQCFastQC Report
Sat 31 Dec 2022
SRR5846260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75001
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC12731.6973107025239662No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT10101.3466487113505152No Hit
ATATGGGGGATACTCATATTGAATATCATATAAAAATAAATGAGGTAAAGAT1770.2359968533752883No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1760.23466353781949573No Hit
CTTTTAGGGAATCGATGGCCCAAATTGTCTTTCTCAAGAAATGTGTATTGCA1510.201330648924681No Hit
ACTGCAGGGGATCTGTGTTGGGATCTCAGCACAGACAGGCATTTAGTGCAAT1490.19866401781309584No Hit
CGGGCGGGGAGATTTGTGAAGGAATAAGCATGATGGAAATAATAGTCTTGAA1380.18399754669937735No Hit
GATAGTGGGTGAATGAATTTATCCATAAAGAGCAAAATAGTTTATCACTACT1350.17999760003199958No Hit
TCTGCCGGGAGATCACCGAAATTGACAAATCTTTAGCTAGATTAAGAAAAAA1310.17466433780882923No Hit
TTGCCAGGGACATACTCACAGTTGATGAGTTGCTGGTCTTTGAGTCCCAGCT1290.17199770669724404No Hit
TGTTGCGGGGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTTG1230.1639978133624885No Hit
CTAGTTGGGATTATCAGCATTTTGGTCAAAGCCATTCAACAAGTCTCTAGGG1220.1626644978066959No Hit
TAACTTGGGGGACCCTATCAACTGGAGAGCCCACAAGGCATAGGGAATAGAG1210.16133118225090332No Hit
GCTACTGGGGGGGAGGCTGTGTGTGGGGAAAGGGACATATGAGAAGTCTGTA1210.16133118225090332No Hit
TTTTCTGGGGTTCTCGGGGAACCTGTATGGCACGAGCAAGGTGGCGGTGCAG1190.15866455113931816No Hit
GAACGAGGGTGCTATAGCTGGGCCCAGTCCTGGCCACGCCGGAGCCTCGGCG1190.15866455113931816No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1190.15866455113931816No Hit
TTATATGGGGAATCGTTTGTGTTAGCAAGTGAACATAATACACCTGTAAGAT1180.15733123558352555No Hit
ACCAAAGGGCAAGAACCAAAATCTGAATATAGTTCACTATTCATATAACAAG1170.15599792002773297No Hit
AACTCAGGGACTTCCAAAACTGAAATGCAAAGAGAATTTAAAAAGCAAAACA1170.15599792002773297No Hit
AATGATGGGAATGTCAATGTATTATCAGGAAACATGTCTATACGATCGCAGA1170.15599792002773297No Hit
GGTATTGGGGATGATTTTCTGGGAGCTATCTTGTCTACCTTGAGGTTCCTGA1170.15599792002773297No Hit
GTTGTAGGGGGGGGAGGGGAAGGAAGGGCCGCTGTCGCCAAGGTTTTCTCTC1160.1546646044719404No Hit
GAAATAGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG1160.1546646044719404No Hit
ATTGCCGGGATGGAAAACTGTATAGAGGTGCCTCCGAAAGCTAATGTTAGAA1160.1546646044719404No Hit
TTGTGTGGGATCCAAAGAGGCTTTATTGAGAGTGAAAAGAAAGACCTCGTGC1140.1519979733603552No Hit
TTCGTGGGTAAGTAAAATACTATTTTTATATTTTAATAGCTAAACATGCTTT1140.1519979733603552No Hit
ACACCTGGGGGGAAATTCCACTGGCTTATTGGGGTATTTTAGATGTTTCTTT1140.1519979733603552No Hit
TAAAGGGAGCACAGATACTACTTTGTTTGACTGATTGCTTTTAAATCCCAAC1130.15066465780456262No Hit
GTGTCAGGGCATATCAACATTAGAACTCCAGTCCCAAACTAATCTGTCAGGT1130.15066465780456262No Hit
ATCACTGGGAGGCATACTTTTTTTAAAAAAAGAGTGTCTATTCTTTAATGAG1120.14933134224877No Hit
GCACCGGGTGAAACTGAGTATCTATAATTAAAACTTGATTGTTATGGAAGAA1110.14799802669297743No Hit
CGACAAGGGGAAAAAGGCATATTGCTTCCAGATTCTGATGTGTGAGGGAAAA1110.14799802669297743No Hit
CTGTTTGGGAAGATAGCATTTCATAATGATGTTTATTTCTCCATTAACATAT1100.14666471113718482No Hit
GCATAGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1090.14533139558139224No Hit
GGCATAGGGACTGTCACTATCATTTGACTTGAACATTTGTGGTATTGTAAAA1080.14399808002559966No Hit
CCAACGGGGCGATCTGATCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1080.14399808002559966No Hit
CATTAATGGGGATGTATACATATGTAACAAACCTGCACGTTGTGCACATGTA1070.14266476446980705No Hit
TTTGCTGGGATTGATTTTCACTGCAGTTGAATGAACAAATTATTTCAGTGTG1070.14266476446980705No Hit
ATCACGGGGTGAAGCATGCAACAAGGCATGAATTTTTGAATTTACAAAATAT1070.14266476446980705No Hit
TATTGCGGGTACTAGAAAACTCTTTTGCATTTTGTAGGAAATATGATAATCA1060.14133144891401447No Hit
TTTGTCGGGCATGTGGAAATGGAGTCCCTTGCATCACTGGAGGTAATTCTTA1060.14133144891401447No Hit
CGAAATGGGGATTGCCCTGACCAGAACTTCCAATACTATGTTGAATAGGAGT1060.14133144891401447No Hit
TAGCAAGGGATGAGGTTAAAGAAAGCATGCAATGCCATCAGCTGAGAAGAAC1060.14133144891401447No Hit
TGGTGTGGGACTCTGATGAAAGAAATCAAAGAAAAACTAAATAAATGGAGGC1060.14133144891401447No Hit
CGCACTGGGATCTAAGAAGTCACTTTCATAGATGTTCATTTTGTAACTGCCT1050.1399981333582219No Hit
TACGCAGGGAAGATATCATCTTGGAATGGGGAAGGGAGGCATGATAGAGCAT1040.1386648178024293No Hit
ATACGCAGGGCGTGTATGTCTGTGGTAGAAATCTGTGGGAGTATGTGAGTTC1040.1386648178024293No Hit
GATTATGGGAAGAATATGGTGGAATAATTGTAGCCTATTGAAAATTCATAAA1040.1386648178024293No Hit
AAAGCAGGGAACTAAGATCTAATAAAGAAACAAATCTTAATTAAAATAAAGA1030.1373315022466367No Hit
GGTAAAGGGATATTATAATAGTAAATACTTTTTAAAAATGGATTTAGAAATC1030.1373315022466367No Hit
CTCTAGTAGGGGAGGTTCAGAAATCAGATGGACCAAACACATGAAAATGTTT1030.1373315022466367No Hit
GAAGATGGGTTGTATGTGTGTGACATTCAGCTAACATTGATCTAGGCACTTA1030.1373315022466367No Hit
TGCCACGGGTTAGTAGCAGGTCAAAAACCTGCCCTCCTGTGACTTATTCCCT1020.13599818669084413No Hit
GAGTCAGGGGAGTTATAATTATTTGAACATATAGATATGTAACATGCCACAA1020.13599818669084413No Hit
CTAAAAGGGAAAGCAAATCTATTAGAAAAAAAGCATTTTTCTAAATGCTCAA1010.13466487113505154No Hit
CAGAATGGGAACATCAAGTTTTCTAACCTATATCTGTTGAGACTTTTTAAGT1010.13466487113505154No Hit
ATAAATGGGGCCCAACAGAGGAACTCTTTCTTTCTAACCTTGTTCAGATTGA1010.13466487113505154No Hit
TTTCTGGGGATGTATAGATCTTCTGGGGGAGGGATGACTGCTTTACAGTATG1010.13466487113505154No Hit
ACTCTAGGGTGACCCTATCAACTGGAGAGCCCACAAGGCATAGGGAATAGAG1010.13466487113505154No Hit
CAGCCTGGGTGACAGAATGAAAGCCTGTCTCTAAAAATAAATAACAAATAAA990.13199824002346636No Hit
AACTGGGGCTAGAACAGAGTCTGGTACAATGGCTCTCGAAATGTGGTCTGGT990.13199824002346636No Hit
GAACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.13199824002346636No Hit
GCGGGACTCTTTTTCTGTTTTATCATGTATGCATTATTTTGTATATGTACAG990.13199824002346636No Hit
TTTGCTGGGGGATCCTTTTAATGGAACAGATTTTATTGACTGCCTCGAAATC990.13199824002346636No Hit
GATCATGGGAATTCTGTAGCAAAGCCAATACAAAAATCAGCTAAAGCTGCCA980.13066492446767378No Hit
TTATATGGGAAATTCAATATGTACCGGCTCTACTTTGGAAGCTCTTATGAAT980.13066492446767378No Hit
GGTTCTGGGGCCCTTGATACTTTTCTGGGGACTGTAATGTAAGCAGAAATTG980.13066492446767378No Hit
GATTCTGGGTATTTACAATAAATGAAGATTACCCTCAAATGCTAGAAGCTGT980.13066492446767378No Hit
CAACATGGGCCCAGCGATGATAATGTACATGAAATTAGCCACGTAACAAGAG980.13066492446767378No Hit
TCTAGCCGGGACAGGTAATCAAATGAGCAGCCGTCTGATGAGCATGCGCAGT980.13066492446767378No Hit
TGTTCGGGGCAAGCTGTTATTTTGCATGTTTATATCTCAGTGGGTATTTATC980.13066492446767378No Hit
AATCAGGGGGCTGTTTAAAGTGCTCTGGGAATGTTTTGAGATCATTGTAAAC970.12933160891188117No Hit
ACTAGGGCTGGAATTGCATGACTTTTGATCAGAACAATGAATATACTGACTG970.12933160891188117No Hit
AGCGTAGGGAGCTGCATTTATTTGACTATGTGATCCCATGTACATGTAAAAA960.1279982933560886No Hit
TTGTTAGGGATCCCAGTCATTAAAATGTTGTGATCTGTTTCTAACATATTTA960.1279982933560886No Hit
GATACAGGGCTGGTATATTTTATCAAACATCCCCAAAGGCTTCTTATGATAA950.12666497780029598No Hit
TGACCGGGAAATGAAAGAAATCAGACATGAAAGACTACATAGTATATGACTT950.12666497780029598No Hit
GCCATTGGGGTCCTTTAATATTCAAATGCATGAACCATCACTCCTTGGCCTT940.1253316622445034No Hit
GTAAGCGGGATGGCTAGCCAGCTCTCCCAGCACCATTAATTAAATGGGGAGT940.1253316622445034No Hit
GTCAAAGGGCAATAGATGGTGAGTTCAGAACTTATTTCAAATTGCTAAGACA940.1253316622445034No Hit
TTTAGCGGGACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGG930.12399834668871082No Hit
CGCAGTGGGCAAGATCTTTTTCTTTGCATTCACAACTTGGCTAACTGGCACA930.12399834668871082No Hit
AGCATCGGGATCTCAGGAGAGGGAACAGTAAATACAAATACCCTAAGCCAAC920.12266503113291821No Hit
AGTCCAGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC910.12133171557712563No Hit
TAAGCTGGGAAGGTATGTTGTTTTCCTTCTCCACTCACCTACCGCACTAAAT910.12133171557712563No Hit
GAGTATGGGGAAAGAAGGTAGTTGAAGCAGAGGATTTCACCAGGGGAAGGAG910.12133171557712563No Hit
ACCTATGGGGTGAACACCATTTTAAGAATAGCATTGGAGAACGAACTATTCT910.12133171557712563No Hit
GAGTCAGGGCGACCAGATGGTGCTGTTCATATATGGATGTAGCTTCTGGGGC900.11999840002133305No Hit
AAGCAAGGGAGGCAAGTTATTTATGAAATGGGCCTTTTGAAAATATATAGCT900.11999840002133305No Hit
GTGAATGGGTGTTCACAATTATTTCCTTAATATCATTAATGTGAGTTCCTTA890.11866508446554047No Hit
ATTGCTGGGATTGCATTCAAATAAGTGATTACTTAAATCATATATCATAGTT890.11866508446554047No Hit
ACTGACGGGCAATTAAAGGGTTCTCTCAACCCTTTAATTATCTACTTTTTTA890.11866508446554047No Hit
TTACGCGGGATTTACAGTGTCCACAGGAGACTTTGACAAAATTTTGTTATTC890.11866508446554047No Hit
ACAGCAGGGTATGTACATTTATAAGGGAAACCACAAAACTTCGATGAAAGAA890.11866508446554047No Hit
GACGATGGGGAGAAATATACATACATGTCAGCAGGCATACATACACATGTGA890.11866508446554047No Hit
TAAAATGGGCCTTGTTTTATCTTTTGTTACCCAACTTACTGTATTTCATTGA880.11733176890974786No Hit
TGCATGGGGACCCACACTGTAGACTCTACCTGCCCATTGCTCATTTAGTAGA880.11733176890974786No Hit
GGCCGCGGGGTTGAAGATGTTTATTCTTTGAGGTTTTATCTTTGGCAAGTTG880.11733176890974786No Hit
TAGCTAGGGACTCAAGAGTATGCAGAGTATCAGAGAAATTTCTACTTAAAAG870.11599845335395528No Hit
ACTTGAGGGATGGCGTAAACATCCAGGTTGGCCATTCCATGGCTCGGAGCAC870.11599845335395528No Hit
GGCCTGGGCGATCTTCCAGCGGGCAGTGTGCAATAAATAAAATTTTGAAGTT860.11466513779816269No Hit
GAAACTGGGATCTCTACATATCAGCATCAAATCACTGATCCATCCTGAAACT860.11466513779816269No Hit
TACTCAGGGAATAGCTCTGTGCCAGGTACATGCAGACACTCAATGTATATTG860.11466513779816269No Hit
AAGATTGGGAGTTGAAATTATTCTTAAAAAACCCCAAATCATGACATGAATC860.11466513779816269No Hit
CTGGCAGGGCAGGAGCTGAAGTCAGGATCAGAAACCACCAACTGTACTGCAA850.11333182224237011No Hit
AGCTTAGGGAACAATGTGCTAGGCACTGTTCCTGCTGACATGTAGCCTTCAT850.11333182224237011No Hit
GACATTGGGAATGTACAGCATCTGTACTTTGTTTGCCTTGATAAAGGTAAGA850.11333182224237011No Hit
GGTTATGGGCAGTGTGGGTCCACAGGGCATAGGAACTTATGTTTTTGTTCCT850.11333182224237011No Hit
AAGGCGGGAGAATCGCTTGAACCTGAGAGGTGGAGTTTGCAGTGGGCCGAGA850.11333182224237011No Hit
GTTTTGGGGATCAACATTTCATTTTTTCTTATGGCCGAATAATGTTCTATTA840.1119985066865775No Hit
CGTACAGGGTGGGCATTTCGATTTATTTCAATGATAACTCACTTGAGAGCTA840.1119985066865775No Hit
ATTGCAGGGGATCTGATATGGGGAGGTAAACTGTGTATACCTACCATTATAT840.1119985066865775No Hit
TTCCTTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG840.1119985066865775No Hit
CTAGCTGGGCTACACATGCAACATATTCTGTGATTGACCATATGCTTGGCCA840.1119985066865775No Hit
GCTCTAGGGAGGCATGGGATATAATCTCCTGGTGTGCCGTTTGCTAAGACCA840.1119985066865775No Hit
TCGTTTGGGTGGTAAAGAAAAACCCATATTTTAAAATTGCTGAAACATTTTG840.1119985066865775No Hit
GTCCCTGGGATGGTGGAAGAGGTTCTCAGCAAGTGTACAGTATTTACCTTCC840.1119985066865775No Hit
CTTGCCGGGATGACAGCTGCATCAGTTGTATTGGATGCAAGTCAACTCTGGA840.1119985066865775No Hit
CATCTAGGGCGATCTCGCTTTGGGAATCATATATGATGTATTTGATCTCTTA840.1119985066865775No Hit
GATGTAGGGCAGCACAAGAGAATTAGAGCTTCAGTGAGAATTTAGAAAAATT840.1119985066865775No Hit
CGACCGGGGGGGAGGTAGGCACTGATACATGTATCTTTTTAATAAACAAGTT830.11066519113078492No Hit
TTCTTTGGGGATGGTGGACCCCTGCATACACGTTCTGGGCAGGCTGCTGCCC830.11066519113078492No Hit
TTCAATGGGTACCGCTGTTCTAGTTCTGTCACTGCTATGTCTTCTTCTCCAG820.10933187557499234No Hit
CAGCGCAGGGAATAGACTAAATGCAAAACCTGAAACTATAATAAACTTCTGG820.10933187557499234No Hit
GAGCAGGGGCTTTCCAAATTGTCTTCTTACACTGTTGCTGAAGGTCACTCTG820.10933187557499234No Hit
CCTCCTGGGACTTGAGTACTTATTGAAATGGACAGTAATAAGCAAATGTTCT820.10933187557499234No Hit
AACCTGGGCAGAAAGTATCTACATTTCCTCTGGCAGTCTAGGTTAAAAAACT820.10933187557499234No Hit
TTATAGGGGAGGAAAAAAGCTGCTAGTCTGGTTTGAATTTCACAATATATAA820.10933187557499234No Hit
GTAAGTGGGCAGGTAGCAATTAAGATGCCAATATGAGAATGTCTTTAGCATA810.10799856001919973No Hit
TAGTCGGGGACCATTTAGAGTTGATATTGACATAAATGTTATTAGCCTACTA810.10799856001919973No Hit
GATGGTGGGGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC810.10799856001919973No Hit
GCTTTTGGGACTCCTCTCCCGGGTCGCTTCCTCTGGAGGCCTTTTATCCTCT810.10799856001919973No Hit
GTCTACGGGACATGTTGGAAATATCACATGAGGTCTATTATAACTAGTGAGG810.10799856001919973No Hit
TATCAAGGGAAGTGTGTGACACTATATTAGATGCCTAATAGGTATTATTTCA810.10799856001919973No Hit
AGACCAGGGAATGCTGGCATAGAGCAGCACTAAATGACACCACTAAAGAAAC800.10666524446340715No Hit
AGACAAGGGCTTTAAGAAAAATGTTCTAAACTGTGTTCATAATATGATGAAC800.10666524446340715No Hit
TAACTTGGGCCAGTATAGTAAAACATTAAACACAAGTAAATGCCAGCTGGCA800.10666524446340715No Hit
CTGTCTGGGTCTGGTGATGGGACCACGATTAATTATATTAAGAATTTTATTT800.10666524446340715No Hit
CCTACAGGGGGAGCCGGGTTGGGGCAGCCATTTTGCCAAGGATCAGAGATTT800.10666524446340715No Hit
CCCATTGCCAAAACTCATCCATCATAGATCAGACAAATACTCGAATAGGACA800.10666524446340715No Hit
TGAGTAGGGATTCCAGAGAATGCCTTATACCTACAATACTTGGCACTTTCCT790.10533192890761457No Hit
ATCAAGGGGTAAAGGGTATACATTAATTGGTACGTAGCATTTAAAATCAGGT790.10533192890761457No Hit
CACATAGGCATTGGCCTGCCTAATACTAGTGTGTTTCTATAACTTCAGATGA790.10533192890761457No Hit
TTTACGGGTGTCTGAATCTAGGTTCTGCTATTGACACATGGTTGCCCTGGAA790.10533192890761457No Hit
CGACTTGGGATCTGCAAGAGATTCTGCAGGAACTGGGTGTGCACACGGTGTT790.10533192890761457No Hit
GTGTGCGGGACCATGAAATTGTCTTACTTGAAAATATTGATCCTGATGAGAG790.10533192890761457No Hit
GAGTAGGGGCATTAGGATAATAGCATAAAATATGAGTAGTCAGTTTCTCTGA790.10533192890761457No Hit
TTAGTCGGGATTCCAATATCAAACAGCAAATTCCACCAATACAAAAAAAATG790.10533192890761457No Hit
CTTATGGGGATTTTTTGCGGTGGAGAAACAAGTGCCTGGGGAGAATATATGA790.10533192890761457No Hit
TTAATAGGGATTTATTGCTAAGTAGGAAAAAAAGGTGCTAAGTAGTGTGTGT780.10399861335182198No Hit
TCAAATGGGATATATATGAAACTTGAAGCATTTATAGATTTCTGTTGTTTTC780.10399861335182198No Hit
TCATATGGGCACAAGAGCTGGCTTAGGTGGGGTAATTAAATTGCCACCTTCG780.10399861335182198No Hit
CTCTAAGTGGGTGCAGCTTTTTTATTGAAATCTGTTGCACATGCTTAAGATT780.10399861335182198No Hit
TCATCAGGGAAGCTTTGCTGTGAATAAAAGGCTTACATTTGTTCCTGCATCA780.10399861335182198No Hit
TGCAATGGGATCCATGAAAAAATAATAGGCTAGCTTATTAACTTATAAACAA780.10399861335182198No Hit
CTCCGTGGGGGAAAAGAAAAAAAATCAATAAGAAGCCAAAGAAAAACTTAAT770.10266529779602937No Hit
CGTCCAGGGCTACAAATCCATCTAATTACCTACATGACCATCATCATCTGGT770.10266529779602937No Hit
AATAATGGGGTTAGTGTTTATGATGTTTTTATCATAAAGGATGTCAGAGAAA770.10266529779602937No Hit
GTAATGGGCACTACAGAATTTTTCTTATAGAAATATTACCAACATTAACTCC770.10266529779602937No Hit
CCCCTTGGGGATAACAGCAGCATATGTACCTCTTCCAACTTACCACAACCTG770.10266529779602937No Hit
CATTGGGGATTGCTAGCTGTCACATAAGGAAAAATGACATCAACTGCAGTAT770.10266529779602937No Hit
GGGTCAGGGGAAGCAAAAATTGACAGAATTGGAGGTAGAAATACATAATGCT770.10266529779602937No Hit
AGCTGGGGTGATGAGAAATACAATGAAGAGGCAATGTCCATCTCAAAATACT760.10133198224023679No Hit
CCGAATGGGCAGTAGCAAGGCAGTCATTTTTTTAAAGCAGTAAGTAACAAGT760.10133198224023679No Hit
ACAAGGGGGAAGACAATAATAACTACATAGGATTGTCAGAAAGATTAAATAA760.10133198224023679No Hit
GCCGGGATAGGCCATTGACTCTTAGCCATCAGAAACAGCCTAAAAATTAATT760.10133198224023679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)