FastQCFastQC Report
Sat 31 Dec 2022
SRR5846257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74306
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT9591.2906091028988238No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC6180.8316959599493985No Hit
GCATAAGGGGGGTCTGATTTTTCAAGAAAGTAAAGGGCAGGACTAGTGAAGT1150.15476542944042204No Hit
GCTGCGGGTGTGAAGGAAATATGTATAAAACAAGCAGGATTATCTTTAATGA1020.13727020698193954No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT990.13323284795305898No Hit
TCCAACGGGCCAAATGGAAAGCGTGGGAGTGGAGACTTGATATTCGGCTTCC960.1291954889241784No Hit
GCGAAAGGGGGGGCTTTATTAGGTGACACTGTGAAACTTCTGATGACAATCT930.12515812989529781No Hit
CAATTGGGGATCCCTACCTGATATTACATCCTATATCTGTTTGTTTTCTGTT920.12381234355233763No Hit
TAAAACGGGAAAGTAGATCCATTAGAAACAGCACATGAGCTTATCTTACCAT920.12381234355233763No Hit
GCATAGGGGGAGCTAATGCCCGATCGACCACTGGCATGGGCTTGGTGTCATC900.12112077086641725No Hit
AGCTTTGGGACTAATGTTTTAATTTATATAAATTGACCCAGAGAAGACTCTC890.11977498452345706No Hit
ATCATGGGAAGGGAGGACTACTGTAGTTGGTCAATGTGATTAGGCCATTTGT870.11708341183753668No Hit
TACGACGGGCAAGTGAGATGGTAGTGTTTGAGTGCTTATTGAGTAAAGCATA850.11439183915161628No Hit
ATGGCGGGGATACTTTATTCTTTTTAGAGTTGAGTAATATTCCACTGAATGG850.11439183915161628No Hit
AGCTAAGGGGAAAAATCATCTCAATAATGTCATGAATGACTATCATTTTTAA840.11304605280865611No Hit
ATTAGTGGGGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCCA840.11304605280865611No Hit
AACTTCGGGCTTGGCTGTTGGAGGAAAAGCAAGTGGCGATTTTATGGTGGCT830.1117002664656959No Hit
TTTTAAGGGGCTGAACCTTGGTGACAGAGTGGGATCCTATCTCAGAATAAAT830.1117002664656959No Hit
TGATAAGGGATATAAATGAAGAAATGAGCTTGGGGAGGCTAAATGTGTCTGC830.1117002664656959No Hit
GTTTTTGGGCATCAGCACCTAGAAAAGATTCTGACATATATTTAGTGCTCAA820.11035448012273572No Hit
ATGTTCGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG810.10900869377977551No Hit
TGTCGCGGGGGGATGTGGAATTTTTATACAAGTTATAAGTGTTTGGCATAGT810.10900869377977551No Hit
GTGACTGGGGGGAAAACTTCCAGGAGAAAATTTGTTAATTAATAGACTTGTA800.10766290743681534No Hit
TTTGCCGGGAACTAAGATCACAATGACTGTGTACCCTTTAAAATATCTTTTA780.10497133475089494No Hit
GCTTGCGGGCTCCACAAATGAAATTGTCACAATGATTCAAAAGAAAGAGATC780.10497133475089494No Hit
ACATTGGGACTCTGGAACATACTTACTTCACATCTGGCAGAGAAAGTTCAAA780.10497133475089494No Hit
TTCAATGGGAAGGGGATCAATTCATATTATCTTGCCTTTCTTTTTGCAACCT780.10497133475089494No Hit
CCGTGGGGGTTCTGAAACAAGAATTGAGATTTCCACAACAATTTTACAGAAT770.10362554840793475No Hit
ATATATGGGCTATGAAAGTCCTAGATGGCATCTTCTTCCTACATAAGGCTGT770.10362554840793475No Hit
TGGCTTGGGGTAAAAAAGCCTAGTGTTGTAAATACAAAACAATCCAAAAATT770.10362554840793475No Hit
TCGCTTGGGATGGATTCAGAAGACAACCATTTCCTGCCACAGTGCTAATAGA770.10362554840793475No Hit
AGCATGGGCAAGTGGTTTATTTGGTGAGAAGATGATCTGTATTTGGTATGGA770.10362554840793475No Hit
TTGTACGGGAACCAAAATATATGCATTTTTCTGGTGTTAGTTCAAGTTTGAT760.10227976206497455No Hit
GCGCAAGGGAGTGGAAAAGAAAATGTATAGTAGTAATAACAGTGGCTTTGGA760.10227976206497455No Hit
ACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA760.10227976206497455No Hit
GTATGGGGGCAAATAGCGGCAGTGGAGGCTCAAGAGGAAAGTTTGTTAGTGG750.10093397572201437No Hit
GCGCCTGGGGCAAGCCCCATACTTGAAAGTTCTGATTCAGCAGGTCTGGAAT750.10093397572201437No Hit
TGTGCTGGGAAAGAGGCAAGGATCTACTGTCATTTGTATGCAATTTCCTGTT750.10093397572201437No Hit
AACAGTGGGGAGCCACCAGAGATTGACAAGAAAAATGAAAAGGTAAGTTTGG750.10093397572201437No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)