FastQCFastQC Report
Sat 31 Dec 2022
SRR5846255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75481
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT9921.3142380201640147No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC6070.804175885322134No Hit
GCATAAGGGGGGTCTGATTTTTCAAGAAAGTAAAGGGCAGGACTAGTGAAGT1110.14705687524012664No Hit
ATCATGGGAAGGGAGGACTACTGTAGTTGGTCAATGTGATTAGGCCATTTGT1100.14573203852625163No Hit
AACTTCGGGCTTGGCTGTTGGAGGAAAAGCAAGTGGCGATTTTATGGTGGCT1020.13513334481525152No Hit
TCCAACGGGCCAAATGGAAAGCGTGGGAGTGGAGACTTGATATTCGGCTTCC1000.1324836713875015No Hit
TAAAACGGGAAAGTAGATCCATTAGAAACAGCACATGAGCTTATCTTACCAT1000.1324836713875015No Hit
GCTGCGGGTGTGAAGGAAATATGTATAAAACAAGCAGGATTATCTTTAATGA930.1232098143903764No Hit
GCGAAAGGGGGGGCTTTATTAGGTGACACTGTGAAACTTCTGATGACAATCT920.12188497767650137No Hit
TTTTAAGGGGCTGAACCTTGGTGACAGAGTGGGATCCTATCTCAGAATAAAT910.12056014096262636No Hit
CACAGCGGGAAATCTGTTCTTCCCATCGCTTCTGCTGTTCTCATGTGTTCTC910.12056014096262636No Hit
ATGGCGGGGATACTTTATTCTTTTTAGAGTTGAGTAATATTCCACTGAATGG910.12056014096262636No Hit
GCATAGGGGGAGCTAATGCCCGATCGACCACTGGCATGGGCTTGGTGTCATC890.11791046753487633No Hit
GCATCGGGGATAATTGTTTTATTTATACTTCTGTACACATATTAAAATTTTC880.11658563082100132No Hit
ATACATGGGGCAGCATGATCCAAAAGAGAATCGCATTTGCTAAATTTTTTGA880.11658563082100132No Hit
TGATAAGGGATATAAATGAAGAAATGAGCTTGGGGAGGCTAAATGTGTCTGC880.11658563082100132No Hit
TAGCTTGGGAGCCCTACATGTCCTCAGATGGGAACGTCTGAGATTCCAGTCT880.11658563082100132No Hit
AAAAGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG860.11393595739325128No Hit
CAATTGGGGATCCCTACCTGATATTACATCCTATATCTGTTTGTTTTCTGTT850.11261112067937626No Hit
AGCTAAGGGGAAAAATCATCTCAATAATGTCATGAATGACTATCATTTTTAA850.11261112067937626No Hit
GGCATCGGGTAATGGGTTAATGAGGAATAATTTGGAAAGAATACTAATTTCA840.11128628396550125No Hit
AGCGCCGGGGGCTTCAACATGAGGAAGGTAACTAAAGGTAACGTCACAATAA840.11128628396550125No Hit
ATATATGGGCTATGAAAGTCCTAGATGGCATCTTCTTCCTACATAAGGCTGT840.11128628396550125No Hit
ATTAGTGGGGGGAATTCCTGTGGCACAATCGGACTTATTCACGCAGTGGCCA840.11128628396550125No Hit
ATACTTGGGATTATAGCTGAATTATATTGGATTTATAAGACTTAAAAGAAAA830.10996144725162622No Hit
ACTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA820.10863661053775121No Hit
GTTCGAGGGCAGTTGACTACTATGTACATCAAGGTTTAAGAATGACTGGCCT810.10731177382387622No Hit
AGCTTTGGGACTAATGTTTTAATTTATATAAATTGACCCAGAGAAGACTCTC810.10731177382387622No Hit
GTGACTGGGGGGAAAACTTCCAGGAGAAAATTTGTTAATTAATAGACTTGTA810.10731177382387622No Hit
GCGCCTGGGGCAAGCCCCATACTTGAAAGTTCTGATTCAGCAGGTCTGGAAT810.10731177382387622No Hit
CAGCCTGGGCAACAAGAGCGAAACTCCGTCTATAAATAAACAAAGAAACAAA810.10731177382387622No Hit
TATGCCGGGGATCGCGGGGGGGTTCAATGCGCCATATTGAAGAGGACGGGTC810.10731177382387622No Hit
ACATTGGGACTCTGGAACATACTTACTTCACATCTGGCAGAGAAAGTTCAAA800.1059869371100012No Hit
TCAGATGGGGCTGTGAGGTAGGAACAATTAGAATTCCTATTTTGAAGATGAG800.1059869371100012No Hit
CAACAGGGAATTTGGTCAACTTTCTTGTAAAACTATGATAATTTTGACTGAT800.1059869371100012No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT790.10466210039612618No Hit
GTGCACGGGGTCGAAAGTTAACCAGCAGAGAGGGGAAGATGGCCCAGGAGCC790.10466210039612618No Hit
TTGGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.10466210039612618No Hit
TGTCGCGGGGGGATGTGGAATTTTTATACAAGTTATAAGTGTTTGGCATAGT790.10466210039612618No Hit
AGTGATGGGAAGGTAAATTATGGGAATCACTGAAATATTTTTGTAGATTAAT770.10201242696837615No Hit
GCTGCAGACATTGGCCTTCTTTAATTCTTTTGCCATTACATACATGTTTCGG770.10201242696837615No Hit
TCACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.10068759025450114No Hit
GTATGGGGGCAAATAGCGGCAGTGGAGGCTCAAGAGGAAAGTTTGTTAGTGG760.10068759025450114No Hit
GGTAGGGGACATAATCTGTGATCCAACAGTTTCACTTTAATGAATTTATGTA760.10068759025450114No Hit
CAGTCCGGGAGGTGACTCTGCTAATCTAACCTGCAGAGCTTTCTTTGGGTAC760.10068759025450114No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)