FastQCFastQC Report
Sat 31 Dec 2022
SRR5846250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75088
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC16042.1361602386533134No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT14731.9616982740251439No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1910.254368207969316No Hit
CTACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.19310675474110378No Hit
CATTTTGGGAACCTGTGTTATTACAGAAAACAATACTAATTGCCTATGAGTA1310.17446196462816962No Hit
CACTTTGGGAGTAAAAGATAGATACCTGGATTCTTGTTTGCATTAGGTTTCC1280.17046665246111228No Hit
GTGCGTGGGCAAGCCAACAAGGGCATAAGGCAATTTGCCTCTATAAATGTAG1280.17046665246111228No Hit
CAGCAAGGGATGCGAGGGCAGTGAAAATAGTGTTTGACTCCATTGACAACCT1270.16913488173875987No Hit
GCGTGGGAGGTAAACACTAGGGTGAACTTTGTGTTAATCTTTCTCATCTTTC1240.16513956957170253No Hit
GACCAGGGGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCC1220.16247602812699766No Hit
ATAGAAGGGGCATGTGAATGTGCACAAGTGCCCTAGATACTGTTCTTCCCCC1220.16247602812699766No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1190.15848071595994032No Hit
AATTGGGGGGCACATTCCTTGTTAAAAGCACGTCTAATTAGTTAAAGAAAGA1160.15448540379288303No Hit
CTGTTTGGGTAGCATCTGGGAATGTTACTGATTTTCATATTTCGACTGTATA1130.1504900916258257No Hit
TACATAGGGAGTTGCTATTGGACTAATTTAATTGTGAAAAAAATTATAATGT1110.1478265501811208No Hit
TCGACAGGGAATGAAAATTCTCTTACAAATATTTTATTTTCTCTTCTCAATT1100.14649477945876838No Hit
AAAAGCGGGCCAGTGATCAATTTTAGAATCAAAAAAGGTGGGACAAACATTA1100.14649477945876838No Hit
GCGCTGGGATTGTAAAAAGAGTAAGAGAGGTTTGGTTTGTGGTGGTTTGCTT1090.14516300873641594No Hit
AACACAGGGTCTCTTCGGGGGGTGAATATAGATTTGATTCAACTTTTAGTTA1080.1438312380140635No Hit
AACCTGGGGGACCCAGACTGTGTGCACAGATGGCTGAAACAGTCAGACCTAC1030.13717238440230128No Hit
AATAGAGGGACTTATGCTGTATATTTGCAGTACTACAAAGAAAATTTTTACC1020.13584061367994885No Hit
AGTGCTGGGTCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGAT1010.1345088429575964No Hit
TGAGTAGGGGAATTTACTATGTAACTCACCCTGTGACTATGAGAAAATCACA1010.1345088429575964No Hit
TTAGTAGGGGGTTCGCTGTATGTTTGCCTTGGACAAAAAGGCTACCTACCAC1000.13317707223524397No Hit
GATTAAGGGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCATG1000.13317707223524397No Hit
TAAATGGGGGAGCCATTGTGCCCGGCCAACAAAACTACTAAAAATAACTATA990.13184530151289156No Hit
CACATGGGGATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTAT980.13051353079053912No Hit
CTTTGAGGGCACGTGAGGACGCACACTGGAGAGAAACCCTGTCAGTGTAAAC970.12918176006818666No Hit
ACGCTCGGGAGAGAGCGGGGTTAGGGCTTCATTGCCTTGTGTCCTCTGCTCC970.12918176006818666No Hit
AAGAAAGGGGAATAAGCTTTTATCAAATAGATAATTGATGCAATTTAGGATT960.12784998934583422No Hit
CGCGAAGGGTCTGACGCTGGGCGATCTAGCGTAGGGAATTCTGTCACTCGAA960.12784998934583422No Hit
TTGCTCGGGTACGTTTTTTCGTCTGAAACAATGGAGGTCAGAAACAAGTTAC960.12784998934583422No Hit
CACAATGGGACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCGCT960.12784998934583422No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC950.12651821862348178No Hit
GTTTCTGGGAATCATATTTGGAGGAAGTTACTAATAGTAGGTTTAATTTGTA940.12518644790112934No Hit
TTTTATGGGAGTAGGTTTGGGCTATAAACAAATTCATTTTCAAAGGTTATCT940.12518644790112934No Hit
TGAGGAGGGAGCCCCACAGAGCAGGTGTTGCCAATGCTGCGGTTCGTGCAGA940.12518644790112934No Hit
TACATAGGGAGACCATTCATGTTTGAAGGGCATAGTGGATACACAATAGTTT940.12518644790112934No Hit
CAGAAAAAAAAAGAAAAGAAAAGAAAGAAAGCGGCTTTGATGTTTTATATTA940.12518644790112934No Hit
AAGCCTAGGAGAGCTACCTTTTGACTAAAAATGTCATTTTTAATGAGCAGTA930.12385467717877689No Hit
GTCTTGGGGGTAGCTTTGTGCTTACAAATATAAGTGAGTCTGAATTGAAAAT930.12385467717877689No Hit
TAGAACGGGAGAGCAGCCTTTGTTCCCTGGCACAATGGGAGCCCAGCAGAGT920.12252290645642445No Hit
GAGACTGGGAGGGCCGAACCCACATGACAAAGAGTGACTCCCTGCCCTCCTT920.12252290645642445No Hit
ACAGTCGGGGAGTGCTTAAGCTAAATGTCATCACAAAAGAGTAAAAAAATTT910.12119113573407202No Hit
CGCAAAGGGAGGCTCACTGAAACTTCTGTCTCCCAGGCTCGAGCGGTTCTCC910.12119113573407202No Hit
GTATCTGGGATTTCTTCAAGGGGTGATGAAAACCTCGGAAGTTTTTAATTTG910.12119113573407202No Hit
AACATGGGATTTCCTAATATGGTAACTAACTCTGCTTTCTAGTATGATTCAG910.12119113573407202No Hit
GCCTATGGGGTTAAAGACTACTACTCTTATTTTCTTCCACCTTTCTGAACTA900.11985936501171957No Hit
GTACAGGGGCACAAAGTGAACATCATTTTATGCTATCTCTTCCAACAAGCAT900.11985936501171957No Hit
ACTAAGGGGATGCATTACCACATTCAAACACAAATCCATTGTAAAATTAGAC900.11985936501171957No Hit
GTCAGTGGATGAACAATAAATAACAACTGTTCTGAGTTAGCTTGAAAGCTCC890.11852759428936716No Hit
ACTAGCGGGGAAAGCAGTGAAAACAGACTTCATTTTCAATAAGTTGGGAGAT890.11852759428936716No Hit
CGCAATGGGAACTAAAATAGTGTTGTCATTGTGGGAATTTGGTTAAATATTT890.11852759428936716No Hit
GCTAAAGGGCGACGTCTTCCGATCTTGTCTAGGGGTGATTTTCCTACAAGGA880.1171958235670147No Hit
CAATATGGGACAACCAGTTGGCAAATATGTCACCTTGGACAAGTCTCTTCAC880.1171958235670147No Hit
TTTCTGGGCTCGGGGAGGGATGGTATTCTACCAAATAATTAAAAACTATACT880.1171958235670147No Hit
GATGCAGGGAGTTGTGCAAAGGCTTTAGTCTGTTACGTATACTATAGACACA870.11586405284466227No Hit
CTGGTGGGATGAGAATTCAGCGAACTTGAAAGAAAAATCATCTGTGAGTTCC870.11586405284466227No Hit
TCAACTGGGGTAAACGATTTGGAAATAGGATGTTTTCAACGTTCTTTTGTCT850.11320051139995738No Hit
GATGTCGGGGCCATCTATTCTAGGTCCCTTGGAATATAATTAAAAACAGGAA850.11320051139995738No Hit
TCGACAGGGCAGTTAGTGGAGTAAGTCATGAAACCCTTAGGAAGAAAAACAA850.11320051139995738No Hit
GTATCCGGGAGAGCTATTGAAAGTAAACTGATGGTTGCCGAGGGCTGGGGGA850.11320051139995738No Hit
TGCAGTGGGATATGCATTATATGCAACTAAAAGTGTGTATTTCTTATGCAGT850.11320051139995738No Hit
GAGCACCGGGATTCTCAGGAAATTCAACCAGAAATGACACTTTGCCCTCCCC840.11186874067760494No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT840.11186874067760494No Hit
TCTATGGGGATGTCTGCATTCTTTTGCAACTGGAAAATTTCATGTTTCTTCA840.11186874067760494No Hit
TGGCAGGGGCTTAAGAGGCAGAGAATGCTGAACTGAAAACTAATTAGGCAAG840.11186874067760494No Hit
GGGCCATGGAGGAGACTCCGTCTCAAACAAACAAAAACCTTCATCTTAAAAT830.1105369699552525No Hit
AAAAGCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA830.1105369699552525No Hit
CGCTGGGGTATTTTGACAGTTTAGGCTTCATTAATAGAACTATTCTGATTAT830.1105369699552525No Hit
GAAAGGGGGCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACTA830.1105369699552525No Hit
GTGCCTGAATTTTCCCAGGCTGTGTTCTCCAACAAGGCAAACCTTCATAGCT820.10920519923290006No Hit
TCAACCGGGTATGATTGTCTTTATCCAAACAGTTTTATTTTAAAATAGACCT820.10920519923290006No Hit
ACAACAGGGGTCTACGTTATAGGGCATCTGTCTCATGAGGCCTCATCATTGT820.10920519923290006No Hit
AACATCGGGTAAAACAAGGGACAGTACTTTGAAGAAGAAATAGGTCTCTATT820.10920519923290006No Hit
TGGATAGGGAAGATATCCAACATATCTTTTATATTGGGCTTATGTCACTTAG820.10920519923290006No Hit
ACAACCGGGACAGCCTAATGTATCAAAACTAACAGAATAAAAATTCTCCAAG820.10920519923290006No Hit
GCTTGAGGGAGAGCTCAGCAGAGATAATTTCTATTTCTTGGAAGTAATAAAC820.10920519923290006No Hit
GTGGTTGGGTAAGTGTTAGAAGTAATAGTTTCTTTTAAAGAGTAACTTCCCT810.10787342851054761No Hit
TCAATTGGGACAAGGCACCTCTGGTAATTAAAAGGTTGAGAACTTCTGGGCT810.10787342851054761No Hit
AAGCCTGGGGGGTGAACTTTGAGCATTAATAGACCTTGGAGCCTTTCCCTAT810.10787342851054761No Hit
AAACACGGGACAGAACATTTTCCTTCATCTGTTCTTTTGTTCGTTTACAGCC810.10787342851054761No Hit
CGGTGCGGGACAGTTTGTTGTCGTGAGATACATCAGACAATATAATCATGGG810.10787342851054761No Hit
ATGATAGGGCTTGCTCCCTAGAAACTGAGATTCCGAAGCAGGTGTTTCCTCT810.10787342851054761No Hit
TCGCAGGGGAGCGCGGAGGGAGGCGCGCGCGGGAGCGAACACCCTCCCGGAT810.10787342851054761No Hit
AAGTATGGGGTTTTGCCAGAAGTTTTTGGATGCAGTTATAACTAGCATTCTG810.10787342851054761No Hit
GCCCGTGGGCAAAAGAAAAATAATATAGTTAAAGTAAGGGGAAAAGTAAAAA800.10654165778819519No Hit
ATCAAAGGGCAGATTTTTCAAAATTGGACTGTTATGTTTTCAGTATTGTTAT800.10654165778819519No Hit
AAACACGGGTATTCAAAGTGATGAGAGATAATACTGTGAGCCAAGAATTCTG800.10654165778819519No Hit
TCTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.10654165778819519No Hit
TATACTGGGTGCCCTAAGGAAGTAGAATCGGATCTACTAAGATTCTTATTTT800.10654165778819519No Hit
TGTTGTGGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGG800.10654165778819519No Hit
ATAACAGGGACTAGTTCTGAACTTTGGACATATTAGTTTCTATGAGCCTCAG800.10654165778819519No Hit
CGTAGTGGGGGAGGAAGATAGCAAATAGGAAACATATTGGTGAGGAAGACTG800.10654165778819519No Hit
ACATCCATGCTCTGAAATATTTAACCACCTTGAAGGGAGGAATGTAGCACAG790.10520988706584275No Hit
TGTGCTGGGAGGCGATCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATT790.10520988706584275No Hit
GGAGCAGGGTATTCATTTCTGTATGTAAAAGAAAAGTTAACTTTATGGTGTT790.10520988706584275No Hit
CCTTGAGGGGGAAGCCTAGAGATCACAGCACGCACTTTCTGGGAGCCCTACA790.10520988706584275No Hit
TCACATAGGGAGCACTTATTGCACTGAATTGTAGTTGTTTACTTGTCTGTGC790.10520988706584275No Hit
AAACTTGGGACATTTCGAGTGGGTACAGTATTCTAACATTCTGCTGGTTAAC790.10520988706584275No Hit
GATTAGGGATGAAATAAGGATTTTCTGCACAGGTAAGTTTGTTTTCATAGTA790.10520988706584275No Hit
AACCGGGAGAAACACAAAGATCAAGGCTCAGCAGCTGATAGACTCAGCAACA790.10520988706584275No Hit
TTTGCGGGGTGGTTTACATCTAAATTAAGGGCAGGAGCTGTCTTTCAGACAT790.10520988706584275No Hit
AAGTATGGGCTGGCTTCTAATCCCAGCTCTTCACCTGACCAGCTGTGTGGCC780.10387811634349031No Hit
ATGCATGGGATTTCCTCTGATGGTACATAGATTTCTCTCTCACTAAGAGGGT780.10387811634349031No Hit
TTTTCACAAGGGCACACTTAATCCTGAGGGTTGTAGAGGGATGAAGATCCAT780.10387811634349031No Hit
GTTAAAGGGTGTCTGTGACTTTTGTGAAAGGGAGAAGTGACATTGCATCAAA780.10387811634349031No Hit
CACAAGGGGCATGTTAAGAATACCCCTGTGGTAACTCAGGAGTCCAGTGGAG780.10387811634349031No Hit
AAGCATGGGGGTGAGAAGATGATCTGTATTTGGTATGGAGCAGGTGGGTGGC780.10387811634349031No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT780.10387811634349031No Hit
CGCTTAGGGGGGCAAAGGCATGACATCTATCTGCAATTATAGTATCATATAG780.10387811634349031No Hit
GTCCATGGGATTCTGACACCAGCTTGCTGCTATGATTTCAGAGCACATAAGT780.10387811634349031No Hit
ACTACGGGGATGCTTCTTTTATTTAAGCACACAGATTCATGACACAAGTACT770.10254634562113787No Hit
TTTACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10254634562113787No Hit
TGTTTAGGGGGGCCACAGTTAAGATATTAATACATTTCTTATTGGAGGTTTT770.10254634562113787No Hit
GATTATGGGCATGGCGGGATGACAACTCTAGTGCAATATTAGAGTTTCATTT770.10254634562113787No Hit
AAATTAGGGAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAA770.10254634562113787No Hit
TTTTGGCCGGGAACTCCTGATTGGTGTTAAGGTGGTAATTTCCCCCATATAA770.10254634562113787No Hit
TCGAGGGCCGATCTAATAGGGGGCAAGGAGAAATAGATCCACAATTACAGAG760.10121457489878542No Hit
GGCTCGGGTAGACACAATAAAAAATGATAAAGGGGATATCACCACTGATCCC760.10121457489878542No Hit
GTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG760.10121457489878542No Hit
AAGTTTGGGTGTTGATTTCTTTCAATGATGCTTACCATCTATTTTAGCCACT760.10121457489878542No Hit
TGTCGCGGGAGCTTATGATCAGGGAAGGGGCAAAGAAGACAGATACCACTAC760.10121457489878542No Hit
ACAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.10121457489878542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)