FastQCFastQC Report
Sat 31 Dec 2022
SRR5846248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160765
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC26921.6744938263925604No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT25321.5749696762354988No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2430.15115230305103722No Hit
TTCCCCTGATCTAGAATGGAAAATACTGTGTTTTTCTCCCTGTAACAGGACA2220.1380897583429229No Hit
CGACTCGGGAGAATGGGAGAAAATATTTGCAAACAATGCATTCAACAATGGC1990.1237831617578453No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1960.12191708394240039No Hit
ATCAGTGGGCACAAATCAGAAGAAAGGTTTAGAAAATTGAGGTCAGGTGGTG1930.12005100612695549No Hit
TAACCGGGGACAACTACTGGTAGTGTTGTTGTGCATTTGCACAAAATAGGTA1910.11880695424999223No Hit
TGTGTGGGGAAAGAAGCAAAATAAAAAGTACTTATAATTGTTTAATACTTGA1850.11507479861910241No Hit
GCTGAGGTGGGAGGATTGCTTGAGCCTGGGAATTTGAGGCTGCAGTGAGCCA1820.11320872080365751No Hit
GTATATGGGATGACTTTAAATTCTTTCCAGAATAAAGAACAATAAAGAGGAA1800.11196466892669425No Hit
TTATGTGGGGGCAACACAGTGAGACTCCATCTCAAAAACTAAACAAAACAAA1730.10761048735732279No Hit
GAGGCCAGCCCATAGCATGTGATTCCAGATTCCTGCGGTCCAGCCTCCAACT1730.10761048735732279No Hit
GCTAATGGGAGATTTGATCAAGATTAAAAGCTGTTATTGATAAAAAGTGATT1720.10698846141884116No Hit
TACACAGGGAACTCTGGGCAATATGGAAAAGTTATTGATTTTGTATATTAAT1710.10636643548035954No Hit
CCAAGTGGGAATATAAAATGGTGTAGCCACTTTGGAAAGTAGTTTAGCAGTT1690.10512238360339625No Hit
GACGATGGGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTC1690.10512238360339625No Hit
GCACCTGGCCTTAATATTCTTTATCATGAATATAATCTGTATTCTTATGTGA1670.103878331726433No Hit
CTAACTGGGACTAGAATCAGATACAAAACTATTAAGTATGTCAACAGCCATT1670.103878331726433No Hit
TCTAGAGGGACCTATCGTTCTACAATAAAATACATGGAAATAGCTTGCTATT1640.10201225391098809No Hit
TCTGCTGGGGAGGACGGGCACAGAGAGGCTGAGTGGGTGGTCTAGATCACAT1640.10201225391098809No Hit
ATTCTTGGGATAATAAAGCACATCACAAATGTTCTTTGCACATAGCTGTTTC1630.10139022797250646No Hit
TTGAAAGGGGTTTTTGAGTTCAATGTAGTCATATTTGTCTATTTTTGCTCTT1620.10076820203402481No Hit
TGCACTGGGGGGGATGGGGATGCTCAGGAAGCCTGTCTCATCTGTCCCTCTC1620.10076820203402481No Hit
CTTGGAGGGGAATTTGTCAGGGGCAGAACTAGAAGTGAGCTCTCTGGACTGC1610.10014617609554319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)