FastQCFastQC Report
Sat 31 Dec 2022
SRR5846247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160263
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC26461.6510361093951815No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT25261.5761591883341757No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2290.1428901243580864No Hit
CGACTCGGGAGAATGGGAGAAAATATTTGCAAACAATGCATTCAACAATGGC2150.1341544835676357No Hit
TTCCCCTGATCTAGAATGGAAAATACTGTGTTTTTCTCCCTGTAACAGGACA2090.1304106375145854No Hit
CCAAGTGGGAATATAAAATGGTGTAGCCACTTTGGAAAGTAGTTTAGCAGTT1990.12417089409283491No Hit
GACGATGGGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTC1880.11730717632890936No Hit
GTATATGGGATGACTTTAAATTCTTTCCAGAATAAAGAACAATAAAGAGGAA1860.11605922764455925No Hit
ATTCTTGGGATAATAAAGCACATCACAAATGTTCTTTGCACATAGCTGTTTC1810.11293935593368401No Hit
TCTGCTGGGGAGGACGGGCACAGAGAGGCTGAGTGGGTGGTCTAGATCACAT1760.10981948422280877No Hit
TATGCAGGGATCTGTGAGAATGGCTATTATCAAAAAGACAGGATAAGTGTTG1720.10732358685410857No Hit
TCTAGAGGGACCTATCGTTCTACAATAAAATACATGGAAATAGCTTGCTATT1710.1066996125119335No Hit
TAACCGGGGACAACTACTGGTAGTGTTGTTGTGCATTTGCACAAAATAGGTA1710.1066996125119335No Hit
TATCACGGGAAGCCCTGAAATGCGAGGGTTTTGCAGACGGTATTGACTCTTC1710.1066996125119335No Hit
TGTGTGGGGAAAGAAGCAAAATAAAAAGTACTTATAATTGTTTAATACTTGA1710.1066996125119335No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1710.1066996125119335No Hit
TTGCTGGGGACCCAAATGTTGAAGCATTGAAAGAGCTGAATACATTGGACTG1700.10607563816975846No Hit
ACAATGGGCTGGCTCGGCAATTCGCTTTATAACCATTCTTATTCCCAAAGTT1670.1042037151432333No Hit
TCTAACGGGAGACATTTGGGTTATTGCTGTCTTTTGGCTATTATGAGTGTTA1670.1042037151432333No Hit
AATTTTGGGGGAATCCAACTTACAAGGAACGTGAAGGAGCTCTTCAAGGAGA1670.1042037151432333No Hit
GTAAGAGGGAACATAAAAACATATTGACTCCTGAATTAGAAATCAGATGCAA1650.1029557664588832No Hit
AAGAAAGGGGCCATTTCTGGAAGTTGGGGTAAAAACGGAAATGGACTATTTT1650.1029557664588832No Hit
TACACAGGGAACTCTGGGCAATATGGAAAAGTTATTGATTTTGTATATTAAT1650.1029557664588832No Hit
TCCAAGGGGGAACATCGGGGCCTTGCTCAGGGGCTGTTGCGTGGTTGCCCTG1650.1029557664588832No Hit
GCTGAGGTGGGAGGATTGCTTGAGCCTGGGAATTTGAGGCTGCAGTGAGCCA1640.10233179211670816No Hit
GATGGGAGAGAAGTTAATTCACTACATACAATGTCCTTTTTAGAGCATTAGG1640.10233179211670816No Hit
GTCGGTGGGCCATATGACTGCCATCTAGATTCTGTAATTCACATTTTGTTAG1640.10233179211670816No Hit
CATAGGCGGGATTTTCTGCCCAACTTTCATTGGTTAAAATATTACTGATCTT1630.10170781777453311No Hit
CTTTGAGGGGAAATCAGAGTTGTAAAGAATGGAATGTGGGAAATAAAGTCAT1620.10108384343235806No Hit
CACACTAGGGACACAATGTACCAGAATCTCTGGGACACAGCTAAAGCAGTGT1620.10108384343235806No Hit
TATCGCGGGATAGCTTGAAATGAGCGAACCCATCCTTGATTTATATACATAT1620.10108384343235806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)