FastQCFastQC Report
Sat 31 Dec 2022
SRR5846246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157927
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC25761.6311333717477061No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT23181.4677667529934717No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2320.14690331608907914No Hit
TTCCCCTGATCTAGAATGGAAAATACTGTGTTTTTCTCCCTGTAACAGGACA2160.13677205291052194No Hit
TTGCTGGGGACCCAAATGTTGAAGCATTGAAAGAGCTGAATACATTGGACTG2040.12917360552660406No Hit
GTATATGGGATGACTTTAAATTCTTTCCAGAATAAAGAACAATAAAGAGGAA1870.11840913839938705No Hit
TAACCGGGGACAACTACTGGTAGTGTTGTTGTGCATTTGCACAAAATAGGTA1860.11777593445072723No Hit
TCTGCTGGGGAGGACGGGCACAGAGAGGCTGAGTGGGTGGTCTAGATCACAT1820.11524311865608794No Hit
TTATGTGGGGGCAACACAGTGAGACTCCATCTCAAAAACTAAACAAAACAAA1810.11460991470742812No Hit
CGACTCGGGAGAATGGGAGAAAATATTTGCAAACAATGCATTCAACAATGGC1810.11460991470742812No Hit
ATTCTTGGGATAATAAAGCACATCACAAATGTTCTTTGCACATAGCTGTTTC1810.11460991470742812No Hit
GACGATGGGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTC1810.11460991470742812No Hit
TACACAGGGAACTCTGGGCAATATGGAAAAGTTATTGATTTTGTATATTAAT1790.11334350681010846No Hit
CACACTAGGGACACAATGTACCAGAATCTCTGGGACACAGCTAAAGCAGTGT1720.10891107916948971No Hit
AAGAAAGGGGCCATTTCTGGAAGTTGGGGTAAAAACGGAAATGGACTATTTT1720.10891107916948971No Hit
TGTGTGGGGAAAGAAGCAAAATAAAAAGTACTTATAATTGTTTAATACTTGA1710.10827787522082989No Hit
CCAAGTGGGAATATAAAATGGTGTAGCCACTTTGGAAAGTAGTTTAGCAGTT1700.10764467127217005No Hit
CTGTCGGGGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGC1700.10764467127217005No Hit
GAGGCCAGCCCATAGCATGTGATTCCAGATTCCTGCGGTCCAGCCTCCAACT1680.1063782633748504No Hit
TTTGGTGGGTGCGCATGGGAGGAATTGAAAGCAAGAAACTGAAGGGAGATTT1670.10574505942619059No Hit
AAGCTTGGGACAGTTGAGGATTCTGAGACTCAGAGACATTAAGTAATTGCCC1670.10574505942619059No Hit
TGCCGGGGTACTCCTGTGTTAACAGTATTTACAATAGTCAAGATGTGGAAAC1640.10384544758021111No Hit
ACAATGGGCTGGCTCGGCAATTCGCTTTATAACCATTCTTATTCCCAAAGTT1630.10321224363155129No Hit
TTGCGCGGGGATGGTTGGTTTATGATTTAGTTGTCACTGAACTGGGTGTTGT1620.10257903968289148No Hit
CCTACGGGGAGGAAAGAAAGTAAAGAATATTTTCTTTATTTACAGAGTTATC1590.100679427836912No Hit
TTTCCCGGGAGACTGTGTTGGTGGCGGTAATTTTGTCCATCAAATGAATATG1590.100679427836912No Hit
CTTTCAGGGTAGGGAGTACTTCATCATGCAACATTTTGCAGCAAAAACCTGG1590.100679427836912No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1590.100679427836912No Hit
ATACGCGGGTGTCCCCCAGTGGGCATAGTCAGTGTCGTTTATATTGTAGCAG1590.100679427836912No Hit
TGTACGGGGAGACAACCCAGGGAAAGAGAGTTATTAGGTGTTTGTTACAGGA1590.100679427836912No Hit
TACAGTGGGAGATTGATGTTTGCATTTTACTTCAGTAAACAATTGCTAAAGA1580.10004622388825217No Hit
CGGTCTAGGTCTGTTTCACCATCTGTTTAAACTAGAATATGTTTAGATTTAA1580.10004622388825217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)