FastQCFastQC Report
Sat 31 Dec 2022
SRR5846236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32177
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT10693.3222488112627033No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC10473.2538769928831153No Hit
TCTTATGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA2220.6899338036485688No Hit
AGTCATGGGAAAATTTATAAGCAAGAAATTATACATAAGTGGTCATGATCTT2080.6464244646797402No Hit
TACTATGGGCAAGAGCCGGGGCCTTTTTGACAGCTTCCGCCGGGACTGCCTG2060.6402088448270503No Hit
TTAAGGGGGATTCACAGAATTGGCAGAGGGGGTCGATTTGGGAGGAAAGGTG1950.6060229356372565No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1900.5904838860055319No Hit
CAGATAGGGGGGAGCGCTTTAGGCTGGCCGGCGGCGCTGGGAGGTGGAGTCG1840.5718370264474625No Hit
TGTTTGGGGTGCTGCTGACCGTGGTTACTTCATCCAGCCCACTGTGTTTGGA1820.5656214065947727No Hit
GAAAAAGGGTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCA1800.5594057867420829No Hit
CGCTAAGGGAAGCCTCTCTGGCACTTTGAGTTAAGTCTATCTGGTATTAAAT1670.519004257699599No Hit
GTACGAGGGAGAGGAAGGCGCTCGGTTACATTGGAGAACTGGAGTGGTCTGG1640.5096808279205643No Hit
AATTATGGGAGCATAACCTACTTGTTTGTATAAATTATTGAAATCCATTTGC1630.5065730179942195No Hit
AACTCTGGGATATTTTTTAAATTATAAAAGCACATCGTTATTTATTTATGAG1590.4941417782888398No Hit
TTTGAGGGGGAGTGGATGCTTTGGCAAGATGGCGGGGAGCGGCGTCCGCCAA1490.4630636790253908No Hit
CAAACAGGGCTGATTCCCAACTGCAATGTCCTCAGTCAGTGTTGTCCCTCTG1480.45995586909904596No Hit
AGCTGAGGGGTGACTGAATCAGCAGTGCCTGCCCTAGTCATAGCTGTGCTTC1460.4537402492463561No Hit
CTATATGGGGACAAAGGCTTGGGAAATGAGGGGAGGTGGAGGCAGGGCAGGG1440.4475246293936663No Hit
ATTGAGGGACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGG1330.4133387202038723No Hit
AAATGAGGGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTC1310.40712310035118254No Hit
ACAACTGGGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCAC1280.39779967057214777No Hit
CTATCTGGGTATTACTAACATTTATCATCGTTCAAATATTTAGTAAAATTTC1270.3946918606458029No Hit
GTGCCAGGGATTCCACAAGTTACCTCATTGTCCTTTAATAATTATCTGCCTA1270.3946918606458029No Hit
TGTTTCGGGTAGCGGTACCATGATAGAATACTGCAATTGTGGTCAGAATTAC1270.3946918606458029No Hit
TACTATGGGGCCCAGCAGGTACTCATAGCCCCCCTGCCCAGATACCATTGTG1240.3853684308667682No Hit
ATAAATGGGGACAGAAGGAATGAACATGGTTTCCTCGCATGGCAGCAGAGCG1200.37293719116138857No Hit
ACATGTGGGTCTGTGGCATGATTATGCATTCCGTAGTATTATTTAATTAATT1190.36982938123504366No Hit
TATGTAGGGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACAAGAAGTAAA1180.36672157130869876No Hit
TGAGCTGGGAATTTCAGATTTTCAGCTGACATTAGCTATAGTATACAGTAGG1160.36050595145600894No Hit
TAGTAGAGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG1160.36050595145600894No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1130.3511825216769742No Hit
TTAAAAGGGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACC1100.3418590918979395No Hit
ACTCTAGGGGGGCGCGGCTCAGGCGTCGGAGCAGGCGGCCGCGGGCGCCGCC1100.3418590918979395No Hit
CTATCAGTGGGGTTGTGCTCAGTTTGCAGTGATACTACTGCAGAGCTTGAAA1070.3325356621189048No Hit
TAAAAAGGGTGAACCAAGCCCAGTTGTTGCAGTGATTGGTTGTCTTTTTCTA1070.3325356621189048No Hit
TTGAATGGGTACAAAAATTAGTAGTGTTTTTATATGCCAATAGTGAAACATC1060.3294278521925599No Hit
AATGCTGGGGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACAC1040.3232122323398701No Hit
TGTTCAGGGATGTTCTGGAAGTAGTGGTGATAGTTGCACAACTGTGAATATA1030.32010442241352516No Hit
CTCGTCGGGCGATCTTTTACGGGGTCTCATTTATGAGAAACCATATGTCAGT1020.31699661248718025No Hit
GCCAATGGGCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTG1000.3107809926344905No Hit
TGGGCCAGGGTACCTATGCTTAAATGTAACAGTCAAAAGCTGTCTGCAAGAC1000.3107809926344905No Hit
TAGTAAGGGTGTGATAGTGGGTAGAAAGGAGAGAAAAACCCACTGAAGGGTT990.30767318270814553No Hit
TTAACTGGGGAAGCATCAGACAGATACTCAAACTCTCACTTTCCCCAAATCA970.3014575628554558No Hit
TTTTAGTGGGATATCAGTAAGTGTAATTTTATTAATTAGTCTCAATGAGCAT940.29213413307642105No Hit
ACAAGAGGGACTTGTAGTCTTTTATGATTACACCAATGTATTCTAGAAATAG900.27970289337104143No Hit
TGCTCAGGGGACGCTCTTCCGATCTTGTGCCGGATACTTGTAGATTTATATT890.2765950834446965No Hit
TCGCTAGGGGGGGGAACAGTCAAGGAAAGCTTCCTCAGCCTTGCTAGAGACC870.2703794635920067No Hit
GCCTTAGGCTGAGGGTAAAAAGCTGGGATTGGTAGGCTGGGTCCAGAACACT850.2641638437393169No Hit
GCTACTGGGTATAGGAAGGTGTTCTTTAGGTATGTTACAGGATTACTTTAAA830.2579482238866271No Hit
ATCGAAGGGACAGTTGGATTTATCTAAGGATATTTCTCAGCATATTAAGGAA830.2579482238866271No Hit
CATCCGGGGGACCTCTGACCCTGCAATGCTGCCTACCATGATTGGCCTCCTG810.25173260403393727No Hit
TTGCGTGGGTATACATGTATATTGGCATGTATGTAGAATATGCATGTCTGGA810.25173260403393727No Hit
TGTACTGGGTAACTATAAATCACTTATTACAGATGCTAAATAGATCACCTTT790.24551698418124748No Hit
CGGTAGGGATACGACCTGATTTCCTTAGGACGGAACCGCAGGCACCTGCGCC770.23930136432855764No Hit
TTCCATGGGTGTCTTCCTTGGGGAATTGTTCATTTAAGGCCTTTGCCCACTT760.23619355440221276No Hit
TTCAGAGGGTACCAGAGGAGCAGTGGAGCCTCATGCAGCACATTATCATTTG760.23619355440221276No Hit
CGTCTGGGTCTTTGTTACAGCTCTAGGAAACTGTAGAAATACAATGCTATGT760.23619355440221276No Hit
AATCATGGGGCCTCCCTCCGGAAAATGGTGAAGAAAATTGAAATCAGCCAGC730.22687012462317804No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCT730.22687012462317804No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT720.22376231469683314No Hit
GTATCGGGCATTAAGTGAAATTGTGCCTTGCCTGAGTGAGCTTCATAAAGCG710.22065450477048826No Hit
AGTTTAGGGCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTC700.21754669484414335No Hit
ACTATAGGGTTTACATAAGATTGGGTTAAATACCATTTGGTTTACTGACAAA690.21443888491779842No Hit
CAAACAGGGTGATTTTATCATTTGTAAAATGGAGATAATGATATTAATAGTT690.21443888491779842No Hit
GCTACAGGGTAAGTAACTGGCTTCAAGGGGTACTGTGGCAACCAAATAAAAT690.21443888491779842No Hit
ATAACGGGGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCA680.2113310749914535No Hit
ACGCGCGGGTTAATGAACTATGAGAATTGGGATTACATCATGTATTTTGCCT670.20822326506510863No Hit
GTGTGAGGGTCTGCAGGCTGTGTAAGCATGTTTACCTGTTGGCTTGCTTTGT660.20511545513876372No Hit
GCTAAAGGGTGTGACCAAGTATTGTCACTCAGCCAACAACTTTTCCACACCT650.2020076452124188No Hit
AGTACAGGGTCAAACATACTTGTAATAACCTGCAGCCATGGGACCTTAAATC650.2020076452124188No Hit
CTAATGCTGGGTATCAAGTACTGACTGTGTGCTACAGCACTTTATGTACATA640.19889983528607388No Hit
GTGCGAGGGACATTTTTCTTCTGAAACTGCAATACTTGCAATTTTTATTCTT640.19889983528607388No Hit
GTCTGAGGGGTAGGAAGTGCATTCTCCATCCTCATCCTCTGCCCTCCCAGGA610.1895764055070392No Hit
TCTTTAGGGATTCTGTACCAAACTGATTTCAAAAGTACTACATTGAAAATAA590.18336078565434938No Hit
TTTCTTGGGATATCAATTTTGGATTATTTTTATGACTTTGAAAGGAGACCTT590.18336078565434938No Hit
TTTTGAGGGTGTGTGAGCAAGCCATCGGCATGTATGTCTGTCTTATTATTAG580.18025297572800447No Hit
AAATAAGGGATTTTGAAGGGTATATTGCTTTGAAGTAAGTCTCAATAAGGCA570.17714516580165957No Hit
TAACGTGGGGAGCGGGAGTCATGGGAACCAGTCAATAATGGGTCAGGCACCT570.17714516580165957No Hit
GTTTCCGGGTGGTGAGGTGTGGATAAGGCTTAGGTGCCAGGCTGTAAGCATT560.1740373558753147No Hit
GATGTTGGGGATGAAAAGAGCACCAGCAGCCCATATGACAAATTTATTGCCA560.1740373558753147No Hit
ATTGTAGGGGTTGGCCGGGCTGGTCTCGAGCTCCGGACCTCGGGTGGTCCGC550.17092954594896975No Hit
TTAGCTGGGGTAAGATTTAGCTTATAAATCATTCAAATTGAAGTGAAAGAAT530.16471392609627994No Hit
TGAGTGCGGGACCATCAAGTCAACATTGACCTTTAAGATTCCTGTTTTGGCC530.16471392609627994No Hit
TAGCCTGGGGAGAGTGTCTAATCCTGCAGTCATGGCGCAGGAAGAGGAAGAT530.16471392609627994No Hit
AGTACTGGGCAAAAAGTAGAAGAGGAAACATTCCCAGACTCATTTTATGAGG520.16160611616993506No Hit
TCACTGGGCAATCACATTGCATTCAATCAATCAGCTGTGATTGATTATGCTT520.16160611616993506No Hit
ATGTAAGGGTGATTATATCTCGATGTCTGTAAAAGATTGCTGTTCTTGGAGT510.15849830624359013No Hit
ACCTCTGGGATTTAGAATATAGAATTGAAATATACACCAACAAAGCACAAGC510.15849830624359013No Hit
ATCCATGGGACCTGTATTCTGATTAAGAGGACTCATTTGCAGGGCGTCTACT490.15228268639090034No Hit
CTTTTGGGGTGTTGACAGAGGATTTATTTAAGCTATTATTTTAATAAAGAAC490.15228268639090034No Hit
CAATAAGGGTGGTTAAGAAGGAAACGGATGGAAAAAATGGCAGAGCAACAAG490.15228268639090034No Hit
GACATAGGGGAAAAAGAAACGAGGACCAAAGCCTTCAACACAGAAACGTAAA480.14917487646455543No Hit
GCTACTGGGCCATACCTCAGTGAAGAAGCCGAAGGATGCTGACACACAGTGA480.14917487646455543No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC480.14917487646455543No Hit
TTAGCAGGGGCCGCTGATGGCGTACTATCTGAAGTGAGGAAAAAACAAGCAG470.14606706653821053No Hit
AAGCTTGGGGATCACTTGAGGTCAGGGGTTCGAGACCAGCTTGGCCACCGTG460.14295925661186562No Hit
TATCAGCGGGATTTGCAGGGTGACAAGTGGCACCCTCCCCAGTCTCTGACAA440.1367436367591758No Hit
AGACATGGGTGTTCACAGGAAAAGAAAAAGGTGTCCAATAAACAGTAAAAAG440.1367436367591758No Hit
TTCCATGGGTGCACTTCCAAATTACTTCTGTCATTTTGTATGTGTTGTGTTT420.130528016906486No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC410.1274202069801411No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC410.1274202069801411No Hit
GGCCCAGGGTATATTTAATGTAGATATATGATAATGTAGATATATATGATAT410.1274202069801411No Hit
AAAAAAGGGCGATCTGTCTTGGGGACAGTATTTCAATAGATATGAATATGAA410.1274202069801411No Hit
TCTTGCGGGTCAGAGGTGGAGCTGGTGCAACTGGTCATCGATGGAGTAAACT410.1274202069801411No Hit
TATGCAGGGTCCTCCTACAGACTGCCAATAAGTTGGAAAAGAAAAAATAGTA400.1243123970537962No Hit
TTTGTTGGGTTGGAAAGGAAGAAATAAAGCTGTGTTTATGTGTAGATGACAT400.1243123970537962No Hit
TTAATGGGATATTAATTTCTGTTCTGATCTTTGTTTCCTTTCTTCTGCTTAC400.1243123970537962No Hit
CGTGATGGGGTAGATTTGCTGTGTCACCTTTAGGGATAAATATACCAAGTTG400.1243123970537962No Hit
TAAGCAGGGCATGCCTCGGCCTCTTGAGTAGCTCGGATTACAGGCACGCACC390.12120458712745127No Hit
TGCGCAGGGTCCTCACCCTTGTTAATCTCAAGAAACTCTTATTTATAATAGG390.12120458712745127No Hit
TTCAATGGGGGTTCTGTGTTTCCTACGGAAATAAGAAACGATAAATATTGCA370.11498896727476149No Hit
AACAAAGGGCAGTTGCCAAAAGCTGCATCAGGGGAATGCGGCAAGGCACACA360.11188115734841657No Hit
TGTGCCTGGGTGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGACGC360.11188115734841657No Hit
TACTAGGGGCTTGGTCAGATTTGAACTCTTCAATCTCTTGCACTCAAAGCTT360.11188115734841657No Hit
TGCATCGGGGTGAGCTCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCGCGCC360.11188115734841657No Hit
TAACTGGGAAGGTCAGGAGATCGAGACCATCCTGGCTAACATGGTGAAACCT360.11188115734841657No Hit
TATCGGGGAGGGGAGGGAGTAAGTTAAACCTTGGACTAGCAAGTAGAAGCCT350.10877334742207168No Hit
ATATAGGGGTAATATGTTACATGTTTTAAACAAACTCTATAGCTTCTGAGTT340.10566553749572676No Hit
TATCATGGGAATATACTCAATGGTGAAAAGTAAAAAGCTTTGACTCTAAGAT340.10566553749572676No Hit
GCAACGCGGGTATGTTAAACTAGCATATCTGCCTTTATTGTGTTTGTCATTA340.10566553749572676No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT340.10566553749572676No Hit
AGTTGGGGTATAAAAGTCTGGTACTGCAGGAAGAGACAGTAAAAATACTAAA330.10255772756938186No Hit
CGCGTAGGGTGTATTGAGCTTTTTCTCAACTTTTTAATTGTAGGTACATGCC330.10255772756938186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)