FastQCFastQC Report
Sat 31 Dec 2022
SRR5846235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31683
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT10623.35195530726257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC10293.247798503929552No Hit
TACTATGGGCAAGAGCCGGGGCCTTTTTGACAGCTTCCGCCGGGACTGCCTG2120.6691285547454471No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT1950.6154720196951047No Hit
TTAAGGGGGATTCACAGAATTGGCAGAGGGGGTCGATTTGGGAGGAAAGGTG1910.6028469526244358No Hit
AGTCATGGGAAAATTTATAAGCAAGAAATTATACATAAGTGGTCATGATCTT1900.5996906858567687No Hit
TGTTTGGGGTGCTGCTGACCGTGGTTACTTCATCCAGCCCACTGTGTTTGGA1880.5933781523214342No Hit
TCTTATGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1810.5712842849477637No Hit
GAAAAAGGGTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCA1810.5712842849477637No Hit
AATTATGGGAGCATAACCTACTTGTTTGTATAAATTATTGAAATCCATTTGC1730.5460341508064261No Hit
GTGCCAGGGATTCCACAAGTTACCTCATTGTCCTTTAATAATTATCTGCCTA1680.5302528169680901No Hit
AACTCTGGGATATTTTTTAAATTATAAAAGCACATCGTTATTTATTTATGAG1650.5207840166650884No Hit
CTATATGGGGACAAAGGCTTGGGAAATGAGGGGAGGTGGAGGCAGGGCAGGG1590.5018464160590853No Hit
AGCTGAGGGGTGACTGAATCAGCAGTGCCTGCCCTAGTCATAGCTGTGCTTC1550.48922134898841646No Hit
CGCTAAGGGAAGCCTCTCTGGCACTTTGAGTTAAGTCTATCTGGTATTAAAT1550.48922134898841646No Hit
AAATGAGGGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTC1530.48290881545308206No Hit
TATGTAGGGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACAAGAAGTAAA1460.4608149480794117No Hit
ACTCTAGGGGGGCGCGGCTCAGGCGTCGGAGCAGGCGGCCGCGGGCGCCGCC1460.4608149480794117No Hit
CAGATAGGGGGGAGCGCTTTAGGCTGGCCGGCGGCGCTGGGAGGTGGAGTCG1440.4545024145440773No Hit
CTATCTGGGTATTACTAACATTTATCATCGTTCAAATATTTAGTAAAATTTC1430.4513461477764101No Hit
CTCGTCGGGCGATCTTTTACGGGGTCTCATTTATGAGAAACCATATGTCAGT1430.4513461477764101No Hit
GTACGAGGGAGAGGAAGGCGCTCGGTTACATTGGAGAACTGGAGTGGTCTGG1400.44187734747340845No Hit
AATGCTGGGGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACAC1390.43872108070574123No Hit
TGAGCTGGGAATTTCAGATTTTCAGCTGACATTAGCTATAGTATACAGTAGG1380.43556481393807406No Hit
TGTTTCGGGTAGCGGTACCATGATAGAATACTGCAATTGTGGTCAGAATTAC1320.4166272133320708No Hit
ACAACTGGGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCAC1280.404002146261402No Hit
CAAACAGGGCTGATTCCCAACTGCAATGTCCTCAGTCAGTGTTGTCCCTCTG1250.3945333459584004No Hit
TAGTAGAGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG1200.37875201212006443No Hit
TTTGAGGGGGAGTGGATGCTTTGGCAAGATGGCGGGGAGCGGCGTCCGCCAA1160.3661269450493956No Hit
ACATGTGGGTCTGTGGCATGATTATGCATTCCGTAGTATTATTTAATTAATT1160.3661269450493956No Hit
ATTGAGGGACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGG1130.35665814474639396No Hit
TAAAAAGGGTGAACCAAGCCCAGTTGTTGCAGTGATTGGTTGTCTTTTTCTA1130.35665814474639396No Hit
ATAAATGGGGACAGAAGGAATGAACATGGTTTCCTCGCATGGCAGCAGAGCG1120.3535018779787268No Hit
TTAAAAGGGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACC1090.34403307767572516No Hit
TGGGCCAGGGTACCTATGCTTAAATGTAACAGTCAAAAGCTGTCTGCAAGAC1060.33456427737272354No Hit
TACTATGGGGCCCAGCAGGTACTCATAGCCCCCCTGCCCAGATACCATTGTG1050.3314080106050563No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1040.32825174383738914No Hit
TTGAATGGGTACAAAAATTAGTAGTGTTTTTATATGCCAATAGTGAAACATC1040.32825174383738914No Hit
ACAAGAGGGACTTGTAGTCTTTTATGATTACACCAATGTATTCTAGAAATAG1010.3187829435343875No Hit
GCCTTAGGCTGAGGGTAAAAAGCTGGGATTGGTAGGCTGGGTCCAGAACACT960.3030016096960515No Hit
TCGCTAGGGGGGGGAACAGTCAAGGAAAGCTTCCTCAGCCTTGCTAGAGACC950.29984534292838433No Hit
TTTTAGTGGGATATCAGTAAGTGTAATTTTATTAATTAGTCTCAATGAGCAT950.29984534292838433No Hit
GCTACAGGGTAAGTAACTGGCTTCAAGGGGTACTGTGGCAACCAAATAAAAT920.2903765426253827No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC900.2840640090900483No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT880.27775147555471386No Hit
ATCGAAGGGACAGTTGGATTTATCTAAGGATATTTCTCAGCATATTAAGGAA870.2745952087870467No Hit
TAGTAAGGGTGTGATAGTGGGTAGAAAGGAGAGAAAAACCCACTGAAGGGTT860.2714389420193795No Hit
GCTAAAGGGTGTGACCAAGTATTGTCACTCAGCCAACAACTTTTCCACACCT840.26512640848404506No Hit
TTCAGAGGGTACCAGAGGAGCAGTGGAGCCTCATGCAGCACATTATCATTTG830.2619701417163779No Hit
AGTACAGGGTCAAACATACTTGTAATAACCTGCAGCCATGGGACCTTAAATC810.2556576081810435No Hit
TTAACTGGGGAAGCATCAGACAGATACTCAAACTCTCACTTTCCCCAAATCA790.24934507464570904No Hit
TTGCGTGGGTATACATGTATATTGGCATGTATGTAGAATATGCATGTCTGGA790.24934507464570904No Hit
GCTACTGGGTATAGGAAGGTGTTCTTTAGGTATGTTACAGGATTACTTTAAA790.24934507464570904No Hit
TGTTCAGGGATGTTCTGGAAGTAGTGGTGATAGTTGCACAACTGTGAATATA780.24618880787804187No Hit
TGCTCAGGGGACGCTCTTCCGATCTTGTGCCGGATACTTGTAGATTTATATT780.24618880787804187No Hit
CATCCGGGGGACCTCTGACCCTGCAATGCTGCCTACCATGATTGGCCTCCTG760.23987627434270742No Hit
GTGTGAGGGTCTGCAGGCTGTGTAAGCATGTTTACCTGTTGGCTTGCTTTGT750.23672000757504025No Hit
CGGTAGGGATACGACCTGATTTCCTTAGGACGGAACCGCAGGCACCTGCGCC730.23040747403970585No Hit
GCCAATGGGCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTG720.22725120727203865No Hit
AATCATGGGGCCTCCCTCCGGAAAATGGTGAAGAAAATTGAAATCAGCCAGC700.22093867373670423No Hit
GTATCGGGCATTAAGTGAAATTGTGCCTTGCCTGAGTGAGCTTCATAAAGCG700.22093867373670423No Hit
CGTCTGGGTCTTTGTTACAGCTCTAGGAAACTGTAGAAATACAATGCTATGT690.21778240696903703No Hit
CTAATGCTGGGTATCAAGTACTGACTGTGTGCTACAGCACTTTATGTACATA680.21462614020136983No Hit
TAGCCTGGGGAGAGTGTCTAATCCTGCAGTCATGGCGCAGGAAGAGGAAGAT670.2114698734337026No Hit
CTATCAGTGGGGTTGTGCTCAGTTTGCAGTGATACTACTGCAGAGCTTGAAA670.2114698734337026No Hit
AAATAAGGGATTTTGAAGGGTATATTGCTTTGAAGTAAGTCTCAATAAGGCA660.2083136066660354No Hit
CAAACAGGGTGATTTTATCATTTGTAAAATGGAGATAATGATATTAATAGTT660.2083136066660354No Hit
GATGTTGGGGATGAAAAGAGCACCAGCAGCCCATATGACAAATTTATTGCCA660.2083136066660354No Hit
TTTCTTGGGATATCAATTTTGGATTATTTTTATGACTTTGAAAGGAGACCTT630.19884480636303378No Hit
TGTACTGGGTAACTATAAATCACTTATTACAGATGCTAAATAGATCACCTTT620.19568853959536658No Hit
ACGCGCGGGTTAATGAACTATGAGAATTGGGATTACATCATGTATTTTGCCT620.19568853959536658No Hit
TTCCATGGGTGTCTTCCTTGGGGAATTGTTCATTTAAGGCCTTTGCCCACTT610.19253227282769939No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCT600.18937600606003221No Hit
ATAACGGGGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCA590.186219739292365No Hit
TGAGTGCGGGACCATCAAGTCAACATTGACCTTTAAGATTCCTGTTTTGGCC590.186219739292365No Hit
TTAGCAGGGGCCGCTGATGGCGTACTATCTGAAGTGAGGAAAAAACAAGCAG580.1830634725246978No Hit
TTTTGAGGGTGTGTGAGCAAGCCATCGGCATGTATGTCTGTCTTATTATTAG560.1767509389893634No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC550.17359467222169617No Hit
ATCCATGGGACCTGTATTCTGATTAAGAGGACTCATTTGCAGGGCGTCTACT540.17043840545402897No Hit
CAATAAGGGTGGTTAAGAAGGAAACGGATGGAAAAAATGGCAGAGCAACAAG540.17043840545402897No Hit
GTTTCCGGGTGGTGAGGTGTGGATAAGGCTTAGGTGCCAGGCTGTAAGCATT530.16728213868636177No Hit
AGTTTAGGGCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTC520.16412587191869457No Hit
GTCTGAGGGGTAGGAAGTGCATTCTCCATCCTCATCCTCTGCCCTCCCAGGA520.16412587191869457No Hit
TAACGTGGGGAGCGGGAGTCATGGGAACCAGTCAATAATGGGTCAGGCACCT500.15781333838336017No Hit
TATCATGGGAATATACTCAATGGTGAAAAGTAAAAAGCTTTGACTCTAAGAT500.15781333838336017No Hit
GTTAGCGGGTGGGGGGCATGGTATTTGCAAAAAAAACAAATTATGGCTAATT500.15781333838336017No Hit
ACTATAGGGTTTACATAAGATTGGGTTAAATACCATTTGGTTTACTGACAAA500.15781333838336017No Hit
TTCCATGGGTGCACTTCCAAATTACTTCTGTCATTTTGTATGTGTTGTGTTT490.15465707161569295No Hit
ATGTAAGGGTGATTATATCTCGATGTCTGTAAAAGATTGCTGTTCTTGGAGT490.15465707161569295No Hit
GCTACTGGGCCATACCTCAGTGAAGAAGCCGAAGGATGCTGACACACAGTGA480.15150080484802575No Hit
TTAGCTGGGGTAAGATTTAGCTTATAAATCATTCAAATTGAAGTGAAAGAAT470.14834453808035855No Hit
GTGCGAGGGACATTTTTCTTCTGAAACTGCAATACTTGCAATTTTTATTCTT470.14834453808035855No Hit
GCAACGCGGGTATGTTAAACTAGCATATCTGCCTTTATTGTGTTTGTCATTA470.14834453808035855No Hit
TATGCAGGGTCCTCCTACAGACTGCCAATAAGTTGGAAAAGAAAAAATAGTA460.14518827131269135No Hit
TCTTTAGGGATTCTGTACCAAACTGATTTCAAAAGTACTACATTGAAAATAA460.14518827131269135No Hit
AAGAATGGGGGTTGCCAACTCGCATGAGCTACCTCCCTCTTTTCATAAAGTA450.14203200454502415No Hit
ACTCATGGGAGGACAGGAGTTGGAGACCAGCCTAGGCAACATGGTGAGACCC450.14203200454502415No Hit
TTTGTTGGGTTGGAAAGGAAGAAATAAAGCTGTGTTTATGTGTAGATGACAT440.13887573777735693No Hit
GACATAGGGGAAAAAGAAACGAGGACCAAAGCCTTCAACACAGAAACGTAAA440.13887573777735693No Hit
GTGCTGGGTAATACTTGGTTGGCAGACTAATTAATTCTTGATAATAATATTT440.13887573777735693No Hit
TGCGCAGGGTCCTCACCCTTGTTAATCTCAAGAAACTCTTATTTATAATAGG440.13887573777735693No Hit
CTCTAAAGGGGTACAGATATTTACCAGAAAGATGAAGATTCTGCAGGGTGCC430.13571947100968976No Hit
ATTGTAGGGGTTGGCCGGGCTGGTCTCGAGCTCCGGACCTCGGGTGGTCCGC430.13571947100968976No Hit
GCACTAGGGTACGTGACAAGCAGTTTTGGAATTTCTTGCAAAGTCAGTATTG430.13571947100968976No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT430.13571947100968976No Hit
TAGTCCGGGTGCACCAAACTTTTTATTTCTTTAAGGTGGGAGTATATTTTTA420.13256320424202253No Hit
AGTACTGGGCAAAAAGTAGAAGAGGAAACATTCCCAGACTCATTTTATGAGG410.12940693747435533No Hit
TCTATGGGGTAGTGGATCTGAGAACACCAATCCCCCATAGATAACTGTACTG410.12940693747435533No Hit
ACCTCTGGGATTTAGAATATAGAATTGAAATATACACCAACAAAGCACAAGC400.12625067070668813No Hit
TACGCCGGGTGCCTGAATTCACTAAAGCCAAGGGTCTGTAAGCCACGCTGCT400.12625067070668813No Hit
GTTTTTGGGTGAGGTCAGGAGTTTGAGACCAAGCTGGCCAACATGGCGAAAC400.12625067070668813No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC390.12309440393902094No Hit
AAAAAAGGGCGATCTGTCTTGGGGACAGTATTTCAATAGATATGAATATGAA390.12309440393902094No Hit
AGTTGGGGTATAAAAGTCTGGTACTGCAGGAAGAGACAGTAAAAATACTAAA390.12309440393902094No Hit
AGACATGGGTGTTCACAGGAAAAGAAAAAGGTGTCCAATAAACAGTAAAAAG390.12309440393902094No Hit
CTTTTGGGGTGTTGACAGAGGATTTATTTAAGCTATTATTTTAATAAAGAAC390.12309440393902094No Hit
AACAAAGGGCAGTTGCCAAAAGCTGCATCAGGGGAATGCGGCAAGGCACACA370.11678187040368652No Hit
TCACTGGGCAATCACATTGCATTCAATCAATCAGCTGTGATTGATTATGCTT370.11678187040368652No Hit
TCTTGCGGGTCAGAGGTGGAGCTGGTGCAACTGGTCATCGATGGAGTAAACT370.11678187040368652No Hit
GCATTTGGGAGCCTTCAATTTTCTTTTTCACTGACCTCTCAGAAAATATCTC360.11362560363601933No Hit
AATGCTGGGGAAAAATACAAGACTCATTCTAGAGTTAAATTACCAATCTTGG350.11046933686835211No Hit
GCATGGGGGCACAAGAATGGCTCACTGTAGCCTGGACCTCCTGGGTTCAAGC340.10731307010068492No Hit
TGCGGCGGGAGCCCTTCTTTGCATTGGTCAGGGTTTGCTGCATTTTCCCAAG340.10731307010068492No Hit
TTAATGGGATATTAATTTCTGTTCTGATCTTTGTTTCCTTTCTTCTGCTTAC340.10731307010068492No Hit
TTCAATGGGGGTTCTGTGTTTCCTACGGAAATAAGAAACGATAAATATTGCA340.10731307010068492No Hit
TATTCAGGGACCCCACCTCTGTGTGTGATGTAGCTTTCTCTCCCTCAGCCTG330.1041568033330177No Hit
AAGCTTGGGGATCACTTGAGGTCAGGGGTTCGAGACCAGCTTGGCCACCGTG330.1041568033330177No Hit
CGTGATGGGGTAGATTTGCTGTGTCACCTTTAGGGATAAATATACCAAGTTG330.1041568033330177No Hit
GATTATGGGACAAGTAGAAATGATTGATAAAACTATTTCCTAAAGCTTTTTA330.1041568033330177No Hit
TATCGGGGAGGGGAGGGAGTAAGTTAAACCTTGGACTAGCAAGTAGAAGCCT320.1010005365653505No Hit
TAAGCAGGGCATGCCTCGGCCTCTTGAGTAGCTCGGATTACAGGCACGCACC320.1010005365653505No Hit
AAACGCGGGTCTCCGTTGGTTTCTAGAGCTCTCTCCCTCCCCTTCTCAGAGG320.1010005365653505No Hit
TATCAGCGGGATTTGCAGGGTGACAAGTGGCACCCTCCCCAGTCTCTGACAA320.1010005365653505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)