FastQCFastQC Report
Sat 31 Dec 2022
SRR5846234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31386
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT10273.2721595615879693No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC9683.0841776588287773No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2170.691391066080418No Hit
AGTCATGGGAAAATTTATAAGCAAGAAATTATACATAAGTGGTCATGATCTT2080.6627158605747785No Hit
TTAAGGGGGATTCACAGAATTGGCAGAGGGGGTCGATTTGGGAGGAAAGGTG2070.6595297266297075No Hit
CAGATAGGGGGGAGCGCTTTAGGCTGGCCGGCGGCGCTGGGAGGTGGAGTCG1910.6085515835085706No Hit
TACTATGGGCAAGAGCCGGGGCCTTTTTGACAGCTTCCGCCGGGACTGCCTG1800.5735041101127891No Hit
TCTTATGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1720.5480150385522208No Hit
AACTCTGGGATATTTTTTAAATTATAAAAGCACATCGTTATTTATTTATGAG1710.5448289046071497No Hit
TGTTTGGGGTGCTGCTGACCGTGGTTACTTCATCCAGCCCACTGTGTTTGGA1690.5384566367170076No Hit
GTACGAGGGAGAGGAAGGCGCTCGGTTACATTGGAGAACTGGAGTGGTCTGG1670.5320843688268655No Hit
GAAAAAGGGTTAGCTGCTGGGACACATGACGGAAGTGTGTATTTTTGGGCCA1650.5257121009367234No Hit
AGCTGAGGGGTGACTGAATCAGCAGTGCCTGCCCTAGTCATAGCTGTGCTTC1640.5225259669916523No Hit
AATTATGGGAGCATAACCTACTTGTTTGTATAAATTATTGAAATCCATTTGC1640.5225259669916523No Hit
AATGCTGGGGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCACCACAC1530.48747849359587075No Hit
CGCTAAGGGAAGCCTCTCTGGCACTTTGAGTTAAGTCTATCTGGTATTAAAT1490.4747339578155866No Hit
CTATATGGGGACAAAGGCTTGGGAAATGAGGGGAGGTGGAGGCAGGGCAGGG1430.45561715414516024No Hit
CTATCTGGGTATTACTAACATTTATCATCGTTCAAATATTTAGTAAAATTTC1400.4460587523099471No Hit
AAATGAGGGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTC1370.43650035047473396No Hit
TTTTAGTGGGATATCAGTAAGTGTAATTTTATTAATTAGTCTCAATGAGCAT1360.43331421652966284No Hit
CAAACAGGGCTGATTCCCAACTGCAATGTCCTCAGTCAGTGTTGTCCCTCTG1340.4269419486395208No Hit
ACATGTGGGTCTGTGGCATGATTATGCATTCCGTAGTATTATTTAATTAATT1340.4269419486395208No Hit
TTTGAGGGGGAGTGGATGCTTTGGCAAGATGGCGGGGAGCGGCGTCCGCCAA1290.41101127891416556No Hit
GTGCCAGGGATTCCACAAGTTACCTCATTGTCCTTTAATAATTATCTGCCTA1280.4078251449690945No Hit
TACTATGGGGCCCAGCAGGTACTCATAGCCCCCCTGCCCAGATACCATTGTG1270.4046390110240235No Hit
TATGTAGGGGCTCAATAGTAGATTTGAACTGGGAGAAGAAACAAGAAGTAAA1260.4014528770789524No Hit
ACAACTGGGCAGTACTCTTAAAACTAGGCGGCTATGGTATAATACGCCTCAC1260.4014528770789524No Hit
ATTGAGGGACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGG1250.39826674313388133No Hit
TAGTAGAGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG1140.36321926973809976No Hit
CTATCAGTGGGGTTGTGCTCAGTTTGCAGTGATACTACTGCAGAGCTTGAAA1140.36321926973809976No Hit
TGTTTCGGGTAGCGGTACCATGATAGAATACTGCAATTGTGGTCAGAATTAC1140.36321926973809976No Hit
TGAGCTGGGAATTTCAGATTTTCAGCTGACATTAGCTATAGTATACAGTAGG1130.36003313579302876No Hit
TCGCTAGGGGGGGGAACAGTCAAGGAAAGCTTCCTCAGCCTTGCTAGAGACC1130.36003313579302876No Hit
ACTCTAGGGGGGCGCGGCTCAGGCGTCGGAGCAGGCGGCCGCGGGCGCCGCC1120.3568470018479577No Hit
TAGTAAGGGTGTGATAGTGGGTAGAAAGGAGAGAAAAACCCACTGAAGGGTT1110.35366086790288664No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1090.34728860001274453No Hit
ATAAATGGGGACAGAAGGAATGAACATGGTTTCCTCGCATGGCAGCAGAGCG1060.3377301981775314No Hit
CTCGTCGGGCGATCTTTTACGGGGTCTCATTTATGAGAAACCATATGTCAGT1050.33454406423246036No Hit
TGGGCCAGGGTACCTATGCTTAAATGTAACAGTCAAAAGCTGTCTGCAAGAC1050.33454406423246036No Hit
TTAACTGGGGAAGCATCAGACAGATACTCAAACTCTCACTTTCCCCAAATCA1030.32817179634231824No Hit
TTGAATGGGTACAAAAATTAGTAGTGTTTTTATATGCCAATAGTGAAACATC980.31224112661696296No Hit
TTAAAAGGGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACC940.2994965908366788No Hit
CATCCGGGGGACCTCTGACCCTGCAATGCTGCCTACCATGATTGGCCTCCTG930.29631045689160773No Hit
GCTACTGGGTATAGGAAGGTGTTCTTTAGGTATGTTACAGGATTACTTTAAA890.2835659211113235No Hit
TAAAAAGGGTGAACCAAGCCCAGTTGTTGCAGTGATTGGTTGTCTTTTTCTA890.2835659211113235No Hit
GCTAAAGGGTGTGACCAAGTATTGTCACTCAGCCAACAACTTTTCCACACCT870.2771936532211814No Hit
TGCTCAGGGGACGCTCTTCCGATCTTGTGCCGGATACTTGTAGATTTATATT870.2771936532211814No Hit
ACAAGAGGGACTTGTAGTCTTTTATGATTACACCAATGTATTCTAGAAATAG850.27082138533103933No Hit
TTCCATGGGTGTCTTCCTTGGGGAATTGTTCATTTAAGGCCTTTGCCCACTT840.2676352513859683No Hit
CGTCTGGGTCTTTGTTACAGCTCTAGGAAACTGTAGAAATACAATGCTATGT830.2644491174408972No Hit
GCCAATGGGCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTG820.26126298349582616No Hit
GCCTTAGGCTGAGGGTAAAAAGCTGGGATTGGTAGGCTGGGTCCAGAACACT810.2580768495507551No Hit
AGTACAGGGTCAAACATACTTGTAATAACCTGCAGCCATGGGACCTTAAATC800.25489071560568405No Hit
ACTATAGGGTTTACATAAGATTGGGTTAAATACCATTTGGTTTACTGACAAA770.2453323137704709No Hit
TGTTCAGGGATGTTCTGGAAGTAGTGGTGATAGTTGCACAACTGTGAATATA760.24214617982539988No Hit
GTATCGGGCATTAAGTGAAATTGTGCCTTGCCTGAGTGAGCTTCATAAAGCG750.23896004588032882No Hit
ATCGAAGGGACAGTTGGATTTATCTAAGGATATTTCTCAGCATATTAAGGAA750.23896004588032882No Hit
TTGCGTGGGTATACATGTATATTGGCATGTATGTAGAATATGCATGTCTGGA730.2325877779901867No Hit
CGGTAGGGATACGACCTGATTTCCTTAGGACGGAACCGCAGGCACCTGCGCC720.22940164404511568No Hit
AATCATGGGGCCTCCCTCCGGAAAATGGTGAAGAAAATTGAAATCAGCCAGC700.22302937615497356No Hit
TGTACTGGGTAACTATAAATCACTTATTACAGATGCTAAATAGATCACCTTT690.2198432422099025No Hit
AAATAAGGGATTTTGAAGGGTATATTGCTTTGAAGTAAGTCTCAATAAGGCA680.21665710826483142No Hit
ATAACGGGGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCA680.21665710826483142No Hit
TTCAGAGGGTACCAGAGGAGCAGTGGAGCCTCATGCAGCACATTATCATTTG680.21665710826483142No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT670.2134709743197604No Hit
ACGCGCGGGTTAATGAACTATGAGAATTGGGATTACATCATGTATTTTGCCT670.2134709743197604No Hit
CAAACAGGGTGATTTTATCATTTGTAAAATGGAGATAATGATATTAATAGTT660.21028484037468936No Hit
TTTCTTGGGATATCAATTTTGGATTATTTTTATGACTTTGAAAGGAGACCTT660.21028484037468936No Hit
TTTTGAGGGTGTGTGAGCAAGCCATCGGCATGTATGTCTGTCTTATTATTAG650.20709870642961833No Hit
TATCATGGGAATATACTCAATGGTGAAAAGTAAAAAGCTTTGACTCTAAGAT650.20709870642961833No Hit
TGAGTGCGGGACCATCAAGTCAACATTGACCTTTAAGATTCCTGTTTTGGCC640.20391257248454725No Hit
TAGCCTGGGGAGAGTGTCTAATCCTGCAGTCATGGCGCAGGAAGAGGAAGAT620.19754030459440516No Hit
CTAATGCTGGGTATCAAGTACTGACTGTGTGCTACAGCACTTTATGTACATA610.1943541706493341No Hit
GTGTGAGGGTCTGCAGGCTGTGTAAGCATGTTTACCTGTTGGCTTGCTTTGT610.1943541706493341No Hit
TAACGTGGGGAGCGGGAGTCATGGGAACCAGTCAATAATGGGTCAGGCACCT610.1943541706493341No Hit
AGTTTAGGGCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTC610.1943541706493341No Hit
GTGCGAGGGACATTTTTCTTCTGAAACTGCAATACTTGCAATTTTTATTCTT600.19116803670426305No Hit
GATGTTGGGGATGAAAAGAGCACCAGCAGCCCATATGACAAATTTATTGCCA600.19116803670426305No Hit
GCTACAGGGTAAGTAACTGGCTTCAAGGGGTACTGTGGCAACCAAATAAAAT580.18479576881412094No Hit
ATTGTAGGGGTTGGCCGGGCTGGTCTCGAGCTCCGGACCTCGGGTGGTCCGC560.17842350092397885No Hit
CAATAAGGGTGGTTAAGAAGGAAACGGATGGAAAAAATGGCAGAGCAACAAG550.1752373669789078No Hit
TTAGCAGGGGCCGCTGATGGCGTACTATCTGAAGTGAGGAAAAAACAAGCAG540.17205123303383674No Hit
TTAGCTGGGGTAAGATTTAGCTTATAAATCATTCAAATTGAAGTGAAAGAAT530.1688650990887657No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCT530.1688650990887657No Hit
ATGTAAGGGTGATTATATCTCGATGTCTGTAAAAGATTGCTGTTCTTGGAGT530.1688650990887657No Hit
AGACATGGGTGTTCACAGGAAAAGAAAAAGGTGTCCAATAAACAGTAAAAAG520.16567896514369462No Hit
GTTTCCGGGTGGTGAGGTGTGGATAAGGCTTAGGTGCCAGGCTGTAAGCATT500.15930669725355254No Hit
GCACTAGGGTACGTGACAAGCAGTTTTGGAATTTCTTGCAAAGTCAGTATTG490.15612056330848148No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC490.15612056330848148No Hit
TGCGCAGGGTCCTCACCCTTGTTAATCTCAAGAAACTCTTATTTATAATAGG490.15612056330848148No Hit
AGTACTGGGCAAAAAGTAGAAGAGGAAACATTCCCAGACTCATTTTATGAGG480.15293442936341045No Hit
GACATAGGGGAAAAAGAAACGAGGACCAAAGCCTTCAACACAGAAACGTAAA480.15293442936341045No Hit
ATCCATGGGACCTGTATTCTGATTAAGAGGACTCATTTGCAGGGCGTCTACT480.15293442936341045No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC480.15293442936341045No Hit
TTCCATGGGTGCACTTCCAAATTACTTCTGTCATTTTGTATGTGTTGTGTTT480.15293442936341045No Hit
GTCTGAGGGGTAGGAAGTGCATTCTCCATCCTCATCCTCTGCCCTCCCAGGA480.15293442936341045No Hit
GCATGGGGGCACAAGAATGGCTCACTGTAGCCTGGACCTCCTGGGTTCAAGC470.1497482954183394No Hit
GTGCTGGGTAATACTTGGTTGGCAGACTAATTAATTCTTGATAATAATATTT470.1497482954183394No Hit
TATGCAGGGTCCTCCTACAGACTGCCAATAAGTTGGAAAAGAAAAAATAGTA460.14656216147326834No Hit
ACTCATGGGAGGACAGGAGTTGGAGACCAGCCTAGGCAACATGGTGAGACCC460.14656216147326834No Hit
GTCACCGGGCAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAA450.14337602752819728No Hit
TCTTTAGGGATTCTGTACCAAACTGATTTCAAAAGTACTACATTGAAAATAA450.14337602752819728No Hit
AAGAATGGGGGTTGCCAACTCGCATGAGCTACCTCCCTCTTTTCATAAAGTA440.14018989358312622No Hit
CGTGATGGGGTAGATTTGCTGTGTCACCTTTAGGGATAAATATACCAAGTTG440.14018989358312622No Hit
CTTTTGGGGTGTTGACAGAGGATTTATTTAAGCTATTATTTTAATAAAGAAC440.14018989358312622No Hit
ACCTCTGGGATTTAGAATATAGAATTGAAATATACACCAACAAAGCACAAGC420.13381762569298414No Hit
TGCGGCGGGAGCCCTTCTTTGCATTGGTCAGGGTTTGCTGCATTTTCCCAAG420.13381762569298414No Hit
AAACGCGGGTCTCCGTTGGTTTCTAGAGCTCTCTCCCTCCCCTTCTCAGAGG420.13381762569298414No Hit
GTTAGCGGGTGGGGGGCATGGTATTTGCAAAAAAAACAAATTATGGCTAATT420.13381762569298414No Hit
CAGGCTGGGTGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAA410.13063149174791308No Hit
TATCAGCGGGATTTGCAGGGTGACAAGTGGCACCCTCCCCAGTCTCTGACAA410.13063149174791308No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC400.12744535780284202No Hit
TACGCCGGGTGCCTGAATTCACTAAAGCCAAGGGTCTGTAAGCCACGCTGCT400.12744535780284202No Hit
GCTACTGGGCCATACCTCAGTGAAGAAGCCGAAGGATGCTGACACACAGTGA390.12425922385777097No Hit
TCACTGGGCAATCACATTGCATTCAATCAATCAGCTGTGATTGATTATGCTT390.12425922385777097No Hit
GATAGGGGTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTC390.12425922385777097No Hit
TTTGTTGGGTTGGAAAGGAAGAAATAAAGCTGTGTTTATGTGTAGATGACAT380.12107308991269994No Hit
GCAACGCGGGTATGTTAAACTAGCATATCTGCCTTTATTGTGTTTGTCATTA380.12107308991269994No Hit
GGCCCAGGGTATATTTAATGTAGATATATGATAATGTAGATATATATGATAT370.11788695596762888No Hit
GCATTTGGGAGCCTTCAATTTTCTTTTTCACTGACCTCTCAGAAAATATCTC370.11788695596762888No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT370.11788695596762888No Hit
TGTGATGGGTCTCCCCACTTATTCAAATAAAAAAGTTTTAATACTATAAACT370.11788695596762888No Hit
TGCACTAGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATC370.11788695596762888No Hit
AGTACAGGGTAATGCCACCAAGGAAGGATCATAGTCTTGTGTTTTCAAATAT360.11470082202255784No Hit
TGGCATGGGAAGTATTTATTTTTCTTTTTTGGTATTATTGTAAAAGTTATTT360.11470082202255784No Hit
AACAAAGGGCAGTTGCCAAAAGCTGCATCAGGGGAATGCGGCAAGGCACACA350.11151468807748678No Hit
AATGCTGGGGAAAAATACAAGACTCATTCTAGAGTTAAATTACCAATCTTGG350.11151468807748678No Hit
TAATTAGGGATGCTGAAAGGCTGTGTGGTGGGAACCAAGAAGCGGGTGCTCA340.10832855413241571No Hit
AAAAAAGGGCGATCTGTCTTGGGGACAGTATTTCAATAGATATGAATATGAA340.10832855413241571No Hit
TCTTGCGGGTCAGAGGTGGAGCTGGTGCAACTGGTCATCGATGGAGTAAACT340.10832855413241571No Hit
AGTTGGGGTATAAAAGTCTGGTACTGCAGGAAGAGACAGTAAAAATACTAAA340.10832855413241571No Hit
TGTGGGGGTACCATCTCATATTAGTCAGAATGGCTACTATTAAAAAGTAAAA340.10832855413241571No Hit
TACTAGGGGCTTGGTCAGATTTGAACTCTTCAATCTCTTGCACTCAAAGCTT340.10832855413241571No Hit
TATTCAGGGACCCCACCTCTGTGTGTGATGTAGCTTTCTCTCCCTCAGCCTG330.10514242018734468No Hit
TGCATCGGGGTGAGCTCGGGAGGTGGAGGTTGCAGTGAGCCGAGATCGCGCC330.10514242018734468No Hit
TAGTCCGGGTGCACCAAACTTTTTATTTCTTTAAGGTGGGAGTATATTTTTA330.10514242018734468No Hit
TTTGCCGGGTCACAAAAGGAAGTTCCAGAGTCCTTCCAGAAGCTTCAGAAAA320.10195628624227362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)