FastQCFastQC Report
Sat 31 Dec 2022
SRR5846233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58932
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC14262.4197380031222426No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT13402.2738070997081383No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2380.4038552908436842No Hit
ATTTCAGGGAACTCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAA1370.23247132288060818No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1360.23077445191067672No Hit
GCATCAGGGCCAGGCAGGACTACAAATGGCCCAGACCCTTCAGAAATGAAGG1290.2188963551211566No Hit
TGCATGGGTACACCATTGGCACAGCACTTAGTAAATCTCAATAGTCCTGTTT1180.20023077445191065No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC1120.190049548632322No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT1070.18156519378266478No Hit
TCGTCCGGGATGGCCTACCTAGTGACAATGGATGGTTGTGAGGATTAAACGA1020.17308083893300752No Hit
AACTTTGGGGAGCTACGACCGCGCCATCACCGTCTTCTCGCCCGACGGCCAC1020.17308083893300752No Hit
ACATGAGGGATTCAGCCATAAGCTAACTTTCCTACAAGTATGAAGGGCATGG980.16629335505328177No Hit
GCCAATGGGGGAACATTTCCCTGTGATATTTCTGTCCTTGATATTCATCAGG960.16289961311341886No Hit
ACGCTGGGGCGAATAAAGCTTTAAAAACCAGGATGTAGAAATTGTAAGAATA960.16289961311341886No Hit
ATACTAGGGGGGGAAATAGTTTCTTTTAGTTATGTATCCCAACTATCTAATC930.1578090002036245No Hit
AGTAATGGGAAATCAGAATTCATTTAGCTCACCACATCTCTTGAATGTGATT920.15611212923369305No Hit
GGCTATGGGAGTTCAAAAGATGCTAGACAATGGCGTCCAAGTTCAGCCTTTG920.15611212923369305No Hit
CTTACCGGGTACATGAAAGCTATTTCATATCTTGGACTCTTTCTTATTCTAG910.15441525826376162No Hit
GTTAAAGGGAAGATTTGCATTAGTTTTTCTCCTGCACCCTTAAAAGTGATTT900.1527183872938302No Hit
AGCTCGGGGAGGTTCAAGGGTACATGTGCAGGTTTGTTAAATAGATAAACTC890.15102151632389874No Hit
TATATAGGGGATCTGAAATAGGGGCTTTCATTGATTTTTGTCAAATTTTTTA860.1459309034141044No Hit
AATTTAGGGACAACATCTTATGTACTCCATAAATGTATACACCTACTATGTA850.14423403244417293No Hit
CCTACATCAAGAAGGAGAGCCCCCTCCTCAATGATTACCGGGGCTTTCTGGC850.14423403244417293No Hit
CTTTATCCGGGGTTATCTCGAGAAGTATTTTATCTTCTGCATTCTTTCTGTT840.1425371614742415No Hit
ACATGGGATACCTTACCCTTAGACATGTAATCTTAAAAAGTTGAGAACTGCC830.14084029050431005No Hit
GAATATGGGCACTTATGTGAAGTCATTTTGAAAACGAGCACTATACAATTAG820.13914341953437862No Hit
CATACGGGGGTTCTACAGGTGGATAAATGAGAATAGTACCAAGAATAGAACC820.13914341953437862No Hit
TTGGTCTGGGTATCCCAGATGTTTATTTATTTTCATTTTCAAACAGAACTTT810.13744654856444716No Hit
ATACAAGGGCAGGAAATCAAAGACCTAAAGACAGAAATACCATTCAACTCAG810.13744654856444716No Hit
CCATAAGGGATGACCCATTTGCCTAACTTTTTTTCAATGTGATGAATGAAAA810.13744654856444716No Hit
CAAGGCGGCATGTGAGTTGTATGTGCCCCCAGTGCTACATACGCAGGTATGC800.13574967759451573No Hit
GATTAAGGGGAAGAAGTGCGGAAGAACAAAGAATCCAAAGACCCTGCTGACG790.13405280662458427No Hit
CTTTATGGGAAGTCAGAGGACAATGGAATGACATCTTTAAAATACTGAAAGC790.13405280662458427No Hit
CATACAGGGCCGTCAGTGATGGCCCCTTCAACAATAGCACTGGGATTCCTTT790.13405280662458427No Hit
TGCTTAGGGTGGCACAGCTTTAGTGGAACACCCCATGGGTGGGAGTGTACAC790.13405280662458427No Hit
GCATGCGGGCTCAATCACTTGGTTCATTGTTCAGCAATATGGCCTAAGGGGG780.1323559356546528No Hit
GGTGGCCGGGACTAGGGGAATTTTTTTTCTTTATTAACAGATAAGAATTCTG780.1323559356546528No Hit
CTTTGGGGGATTATTTTGGCCTGAATGCTGTCATATATGTAACATGTTAGTA780.1323559356546528No Hit
TCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTG770.13065906468472135No Hit
TCGCTGGGGGCACGTTAAGCCATGCTGTAAGCATTGTTTTTATTTTCAGGCT770.13065906468472135No Hit
TCATTTGGGACTTGAGGTCTTGCTTAATCACTTTTGTTGTCCTGAGGACTTG760.12896219371478992No Hit
GCTCAAGGGCTGGCCTTTGCAAAGCACGTCTGAAGAAATGAGCACTTTCTAT750.1272653227448585No Hit
CAGTCGGGGGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTT750.1272653227448585No Hit
GCTTATGGGATGAAACTACTGGAATAAAACTGGACTAGGCAAAAATTTCTTG750.1272653227448585No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT740.12556845177492704No Hit
ATTTAGGAAGAGAGCAACAAATTTGATTATATTTTTAAGTGTCTTAGATCCT740.12556845177492704No Hit
ATGACTGGGACATTCATGTGATGATGCTCAATTGCTTATGTCAGTGGAATGT730.12387158080499559No Hit
AGTTGGGGGAGACACACAAGAAATGTGTTATAGGTTCCCAAACATTCTTCAT730.12387158080499559No Hit
GCCTTGGGATATGAATCATGAATCAAGAGGCTTTACTCTTTTTCCCTCAGTT730.12387158080499559No Hit
TTGACAGGGATACAAGGATCAACATTATTTAAAAATTATACATATTAACTCT730.12387158080499559No Hit
CTGAGAAGGGAAGAGGAAAAAGAAAGGAAAAGATAAAACTCCTGCCTGAGAA730.12387158080499559No Hit
CCCAGGTTCTTGAGAGGCTGAGGCAGGAGGATTGGTTGAGCCCAGGTGTTGG720.12217470983506415No Hit
TGACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.12217470983506415No Hit
ACGAACGGGGCCTGTTTTCTCAACTGTAAAATGGGCTGAACAATGCACTCCT720.12217470983506415No Hit
ACTCTAGGGGACTGGGTAATTTATAAAGAAAAAGAGGTCTAACAGACTCACA720.12217470983506415No Hit
GCCTAAGGGAGAATTTTACTTCATTGCAGAAAGGGTAGCTGCCTCCTCAAGG710.12047783886513269No Hit
GTGGCCGGGATCCTGTTATGTTAAGGGAAATCCAAATTCCCAATTTTTTTTG710.12047783886513269No Hit
GCCAAGAGGGAACACTCCATTTCTCATTTTAAAAATGAGGAAATGAAGTATA710.12047783886513269No Hit
ATCTACGGGAATGAGCCTAAACTGACTCCGTTGGGCCAACACCTTGCAGCTT700.11878096789520123No Hit
ATAATTGGGAGGTGTCTGGTGAGGGTCCAGTCTCTGGTTTCAAGATGGCACC700.11878096789520123No Hit
ACGCTAGGGAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCA700.11878096789520123No Hit
CTTGTTGGGGGAAAATCATGCCTCCAAATTTTTATTTTTCAGCTTAAAAAAT700.11878096789520123No Hit
TGCCTTGGGATATACAAATATATAGACATCGCATGACCACATATTAAGTATG690.1170840969252698No Hit
AATGCCGGGGGGTCGTTATTCATTCCAGACAGGTTGGCAAAATAGCTATAAT690.1170840969252698No Hit
CGTATGGGGGTAAATATTTATCAATAAAAATAAACATTTTGTTTTTCTAATA690.1170840969252698No Hit
CGCATTGGGGTATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCA690.1170840969252698No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT690.1170840969252698No Hit
AGTCTAGGGCTTTCTGGCATGGATCAAGAGAAAAGAAAATACAAGAAAACAG680.11538722595533836No Hit
CATCATGGGAGTACTCTATTCTTCAAATATCCGTTTCTTGGCATCGGGCATG680.11538722595533836No Hit
ATTTTAGGGTATGATTAGGTGACGAGTTGACATTGAGATTGTCCTTTTCCCT680.11538722595533836No Hit
TAAATGGGGGATTGCTGGGTTGAATAATAATTCTGTTTTAAGCTCTTTAACT680.11538722595533836No Hit
GGCTCAGGGAATCTATATTTTTAAAGTATTACATCATGTTCACTCCAAGAAG680.11538722595533836No Hit
AAGGCAGGGATGTGGAAATTTAATAATATATTTTTGAGCGACCAATGGGACC680.11538722595533836No Hit
ATTTTTTGGGTCTAGACAGAAATTAACAAAGAAATATTGAATTTAAACTGCA670.1136903549854069No Hit
ATTTAAGGGCCTCCATGTTCATTAACTTTCCACTGATTTAACAGTTCTAACC670.1136903549854069No Hit
CTGCCTGGGGTACCCTGAAATACAGGTTTCTGACAACTTTAAGATCATACAA670.1136903549854069No Hit
AAGCCTGGGGCAAGAGAAAGAAGCACTGTTGCAAGAATTCAATTCCGTCTTC670.1136903549854069No Hit
AGCGATGGGACTTTAAGGAAACAAACAAGATTACAACAATTAAAAAAACACA670.1136903549854069No Hit
CGATAGGGACCATGCTAACAGTAAGCATAAGAAAGCTGCAGTAGCTATAGTA670.1136903549854069No Hit
AGTAATGGGAACCTGAAGATGGATTTTTTTTCAACAAGAAGATAGATGGGAG670.1136903549854069No Hit
TCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCC660.11199348401547546No Hit
GTTTGAGGGACTTCTTATGAAGTTGGCATTACCTGAGACTCTTAGCTGTGAT660.11199348401547546No Hit
GGCAACGGGACTACAGGTTGAGTATCCCTTATCCGAGAGCCTTGGGACCAGA660.11199348401547546No Hit
ATGGTAGGGTGGTTTTGTCCGTAGAGTATTAAGAATATATCCCTGACTTATA660.11199348401547546No Hit
ACAGTGGGGCTGATCTCTGGGACACAACTAAAGCATTGTATAGAGGGAAATT660.11199348401547546No Hit
TAAATGGGGACAGACTGCTATACTGGACATTTTAAATGCATGTCTAAAGGAA660.11199348401547546No Hit
ATGAAGGGGAATTTGTGCAGGAGAAGGACTTGCCCGCATGGAGCTATTTTTA660.11199348401547546No Hit
TGATATAGGATACATTGTGAATCTCTAAATCCACGGAAATGATATAAGAAAA650.11029661304554403No Hit
TGCAAAGGGACTGTGAATGTTGTATGGAATTGATAGAACAATTTCTTTTTTA650.11029661304554403No Hit
GGTACTGGGCCTCACAAACTTCTTTACGAAGAGTGAAATATGTTTTTGTACC650.11029661304554403No Hit
GGCTCTGGGCCTACTGGTGTGAATATGGAGTCAAATAAAAATCCAGTTTACA650.11029661304554403No Hit
GGCAACGGGGCCAGCCCTAATAAGAAGCATGCTAAAGCCACAGCAGCTACTG650.11029661304554403No Hit
TAAAACGGGTGTTTCTTCTCCACTCAAAGTTACTCATTCCCTGTAGCTGAAG640.10859974207561257No Hit
TTAACTGGGCAGTTATATCAAAAACAGATTCACATTTTGATTGGTGGGAAGG640.10859974207561257No Hit
GTACATGGGAGCAGGTTCCTCTTCTGGAATATTAAAGAGGAAAACGTAGAGG640.10859974207561257No Hit
AACAATGGGATTGCCTAGTGACGTCTTAGCCATTGTAATGTTGTAGCACAAG640.10859974207561257No Hit
ATTGAAGGGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAAAAGAGAAAAAAG640.10859974207561257No Hit
ACAATAGGGCTCTAAATTTTCTAAGCAGGAAAGCAATGTAAACCAAAAATCA640.10859974207561257No Hit
TGTACGTGGGGATACAGCTAAGGGAAAAAATAGTGAGCTTGAAGATACGTCT640.10859974207561257No Hit
TTGTAGGGCAAGGAAGTTACAATACAATGTTGAATTATGTATTCTACTTTAA640.10859974207561257No Hit
CTCACTGGGCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAA630.10690287110568111No Hit
TAGTCTGGGAGTTTACTAATCAGTACATTTGTTTTAATAATATATTCCCATA630.10690287110568111No Hit
ACATGCGGGAATGACTCGTAAGATACCATAGTCTGATTTTTCTCACATTAAA630.10690287110568111No Hit
ATGTTAGGGCAAAGGCAATGGATGGGTACCATTTTAGAATGAAAAATTATAG630.10690287110568111No Hit
ATGCAGGGGATTTCTACCTTCATTTCCAACTTTGTATTGTGCTTTGAAAACC630.10690287110568111No Hit
ACATGGGGCCTGTGAGTATGTGTGGGGGACAGCTGATAGAAGGGAGGAGAGT630.10690287110568111No Hit
TTTTTTGGGATGTGCATCTTTTGTCGAATGTTTTGGTTCAAGAAAGAATGTT630.10690287110568111No Hit
TCTAGTGGGATGTAAAGACCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAA630.10690287110568111No Hit
AAATATGGGCTTCATTTCCCATTCATGCATTCAAAGACACAGGGTAATGCAC630.10690287110568111No Hit
AAAGCTGGGATGCAATTTATGACAAAATTATTTATGATAATTGGATGGCTGC620.10520600013574968No Hit
AAGCACGGGATACCCTCTTTTCAACTGTCATATATTTGACCTTCACTAGATT620.10520600013574968No Hit
GCAAAAGGGAAGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTTTGTTG620.10520600013574968No Hit
GAGCAAGGGCTTGGCTATAAGCCATTCTTTGGAAACTCTCCGAGTACCTGAA620.10520600013574968No Hit
TTCATGGGGAGTCAAACAAGTTGTGCCTTTCACAGCTTCTTTACTACTGCAA620.10520600013574968No Hit
TCTCCTGGGATAGTAAGTGACTCACAAAAAATCAGTGCTTACTATTCATCTG620.10520600013574968No Hit
GAATATGGGCTGAAGCGGGGATCTTGTCCCACTGAATTGCCCTGGAATTAAT620.10520600013574968No Hit
TGACAAGGGGAAAAGGTTTGAAAACATACAAAAGCACACATATACTAAATTT610.10350912916581824No Hit
GCTTATGGGCTGTGAGCTTGGCAAGGCAGAGGAAGCTCCTTATGGCCTGAGA610.10350912916581824No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC610.10350912916581824No Hit
ACTAAAGGGATCTATTTCTGTGTTATTCTAGCGATGGATTTGTGCCGACAGT610.10350912916581824No Hit
GTTAATGGGATGTGGAAGATTGTTATTTCATTAGGATTTAGTAAAATTTTTT610.10350912916581824No Hit
TGCAGTCCCAGCTACGAGGGAGGCTGAGGTGAATCACTTGACCCCAGGTGTT610.10350912916581824No Hit
CACACTGGCTTATATTCATGGGATAGAAACATAAAGGATATCTAGCAGGTAT610.10350912916581824No Hit
TCGGATGGGCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGATC610.10350912916581824No Hit
CGTCAAGGGTAAGCCAATGAGTCATGATTGGAATTGAACTTGGAGAATATCA610.10350912916581824No Hit
GCTGCAGGGTTATTTTGAGCTTTGCAAGGAAAGTTAAATTCACCATTACCAC610.10350912916581824No Hit
GGAGTAGGGAAACTTACTACTTGATGGAGTGAGCTATTCTTATGGTAAGCCG600.10181225819588678No Hit
GTAGGAGGGATCATGAACACAGTAACTGATAGGTAAAAAGACTGCATGATTC600.10181225819588678No Hit
TTTTTGGGGAGGATTTTAAATTACACAGTTCACAAACAGTAAAGGCCATGTG600.10181225819588678No Hit
TGGGATGGGCAGAAGAAGAAAGATCTAAAATCAGTAATCTAAGCTTCTACCT600.10181225819588678No Hit
CCACCCGGGCACTATCCGTGATCCAGTCTTAATTTGAGCATGAGAGCAAAAT600.10181225819588678No Hit
GTCTCAGGGCTCGTAGGAAATGTATCGGTGTGGGAGGCCTGGGGTACGTGAG600.10181225819588678No Hit
GTTCTGGGGATAGGCAAAGAAGAATGGAGAAAAGAAATTGTAAATGGTTCTC600.10181225819588678No Hit
ACTGGAAGAAAGAGTATCAGCGATGGATGATGAAATGAATGAAATGAAGCGA600.10181225819588678No Hit
TCCAAAGGGTGCTTCCTGAACAGTATTAAATTATATACTTAATTTTGACTAA600.10181225819588678No Hit
TGATAAGGGCAAGCCAAATGTCCAACAATGATAGACTGGATTAAGAAAATGT590.10011538722595532No Hit
GTTACAGGGCGCTGTATTTAAAATAAACTACTCCATAGCTCTCTTCCCTAAT590.10011538722595532No Hit
AGCCTGGGTAACAAGAGGGAAACTCCATCTCAAAAAATAAGTTAATTAAATT590.10011538722595532No Hit
GGCGCTGGGGATTAGTTTTAAGACAGGACTTTTTAAATTTACAATATTTTTG590.10011538722595532No Hit
GTTGAGGGGGGTGTCACTGAGAACTAAGCTTTTGAGGAAAATAAATATAGAT590.10011538722595532No Hit
CTGCAAGGGAAGATGAAATCATATTCCATGGGAGAATTCTCTTCTCTAGAGA590.10011538722595532No Hit
CCCGATGCGACCCAGTTTATAGAGTTTGCCAAACTTTCTGATTTTGCAGATG590.10011538722595532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)