FastQCFastQC Report
Sat 31 Dec 2022
SRR5846232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58428
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC13522.31395906072431No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT13062.2352296843978916No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2600.44499212706236735No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1630.2789758335044841No Hit
ATTTCAGGGAACTCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAA1380.2361881289792565No Hit
GCATCAGGGCCAGGCAGGACTACAAATGGCCCAGACCCTTCAGAAATGAAGG1310.2242075717121928No Hit
TGCATGGGTACACCATTGGCACAGCACTTAGTAAATCTCAATAGTCCTGTTT1300.22249606353118367No Hit
ACGCTGGGGCGAATAAAGCTTTAAAAACCAGGATGTAGAAATTGTAAGAATA1100.18826589991100157No Hit
AACTTTGGGGAGCTACGACCGCGCCATCACCGTCTTCTCGCCCGACGGCCAC1070.18313137536797425No Hit
GCCAATGGGGGAACATTTCCCTGTGATATTTCTGTCCTTGATATTCATCAGG1030.17628534264393783No Hit
CCTACATCAAGAAGGAGAGCCCCCTCCTCAATGATTACCGGGGCTTTCTGGC1000.17115081810091054No Hit
ACGAACGGGGCCTGTTTTCTCAACTGTAAAATGGGCTGAACAATGCACTCCT1000.17115081810091054No Hit
TCGTCCGGGATGGCCTACCTAGTGACAATGGATGGTTGTGAGGATTAAACGA980.16772780173889232No Hit
ACAGTGGGGCTGATCTCTGGGACACAACTAAAGCATTGTATAGAGGGAAATT970.1660162935578832No Hit
ACATGGGGCCTGTGAGTATGTGTGGGGGACAGCTGATAGAAGGGAGGAGAGT960.1643047853768741No Hit
GGCTATGGGAGTTCAAAAGATGCTAGACAATGGCGTCCAAGTTCAGCCTTTG950.162593277195865No Hit
ATGACTGGGACATTCATGTGATGATGCTCAATTGCTTATGTCAGTGGAATGT930.15917026083384678No Hit
TGTACGTGGGGATACAGCTAAGGGAAAAAATAGTGAGCTTGAAGATACGTCT930.15917026083384678No Hit
GAATATGGGCACTTATGTGAAGTCATTTTGAAAACGAGCACTATACAATTAG920.15745875265283768No Hit
TCATTTGGGACTTGAGGTCTTGCTTAATCACTTTTGTTGTCCTGAGGACTTG910.15574724447182858No Hit
AATTTAGGGACAACATCTTATGTACTCCATAAATGTATACACCTACTATGTA900.15403573629081946No Hit
ATCTACGGGAATGAGCCTAAACTGACTCCGTTGGGCCAACACCTTGCAGCTT890.15232422810981036No Hit
GTTCTGGGGATAGGCAAAGAAGAATGGAGAAAAGAAATTGTAAATGGTTCTC880.15061271992880126No Hit
AGTTGGGGGAGACACACAAGAAATGTGTTATAGGTTCCCAAACATTCTTCAT870.14890121174779217No Hit
CAGTCGGGGGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTT860.14718970356678304No Hit
ACATGGGATACCTTACCCTTAGACATGTAATCTTAAAAAGTTGAGAACTGCC860.14718970356678304No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC860.14718970356678304No Hit
TCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCC850.14547819538577395No Hit
AATGCCGGGGGGTCGTTATTCATTCCAGACAGGTTGGCAAAATAGCTATAAT850.14547819538577395No Hit
TCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTG850.14547819538577395No Hit
AGCTCGGGGAGGTTCAAGGGTACATGTGCAGGTTTGTTAAATAGATAAACTC840.14376668720476485No Hit
GTAACAGGGCAACCAATTTTGCCATGAAAAGGGATGTCCAGGAAGGTCAGGC840.14376668720476485No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT830.14205517902375575No Hit
ACATGAGGGATTCAGCCATAAGCTAACTTTCCTACAAGTATGAAGGGCATGG820.14034367084274663No Hit
ATACAAGGGCAGGAAATCAAAGACCTAAAGACAGAAATACCATTCAACTCAG810.13863216266173753No Hit
GTCAGTGGGCAATATAGTAATGCTTTTAGTAGAGTACTAGATAGTATCAGTT810.13863216266173753No Hit
CCATAAGGGATGACCCATTTGCCTAACTTTTTTTCAATGTGATGAATGAAAA810.13863216266173753No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT800.1369206544807284No Hit
ATACTAGGGGGGGAAATAGTTTCTTTTAGTTATGTATCCCAACTATCTAATC800.1369206544807284No Hit
TAAATGGGGGATTGCTGGGTTGAATAATAATTCTGTTTTAAGCTCTTTAACT800.1369206544807284No Hit
GTTAAAGGGAAGATTTGCATTAGTTTTTCTCCTGCACCCTTAAAAGTGATTT800.1369206544807284No Hit
CTTTATCCGGGGTTATCTCGAGAAGTATTTTATCTTCTGCATTCTTTCTGTT800.1369206544807284No Hit
AAGGCAGGGATGTGGAAATTTAATAATATATTTTTGAGCGACCAATGGGACC800.1369206544807284No Hit
TGCTTAGGGTGGCACAGCTTTAGTGGAACACCCCATGGGTGGGAGTGTACAC800.1369206544807284No Hit
GTAACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.1352091462997193No Hit
ATTGAAGGGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAAAAGAGAAAAAAG790.1352091462997193No Hit
GCCTAAGGGAGAATTTTACTTCATTGCAGAAAGGGTAGCTGCCTCCTCAAGG770.1317861299377011No Hit
TTGGTCTGGGTATCCCAGATGTTTATTTATTTTCATTTTCAAACAGAACTTT770.1317861299377011No Hit
AAGCCTGGGGCAAGAGAAAGAAGCACTGTTGCAAGAATTCAATTCCGTCTTC770.1317861299377011No Hit
ATTTAGGAAGAGAGCAACAAATTTGATTATATTTTTAAGTGTCTTAGATCCT770.1317861299377011No Hit
GCTCAAGGGCTGGCCTTTGCAAAGCACGTCTGAAGAAATGAGCACTTTCTAT760.130074621756692No Hit
TATATAGGGGATCTGAAATAGGGGCTTTCATTGATTTTTGTCAAATTTTTTA760.130074621756692No Hit
AGTAATGGGAAATCAGAATTCATTTAGCTCACCACATCTCTTGAATGTGATT740.12665160539467377No Hit
TGATATAGGATACATTGTGAATCTCTAAATCCACGGAAATGATATAAGAAAA740.12665160539467377No Hit
GTACATGGGAGCAGGTTCCTCTTCTGGAATATTAAAGAGGAAAACGTAGAGG740.12665160539467377No Hit
TTGACAGGGAGATTAGGGTTCTCTGGGCATAAAACACATAGATGACAACAGG740.12665160539467377No Hit
CACACTGGCTTATATTCATGGGATAGAAACATAAAGGATATCTAGCAGGTAT740.12665160539467377No Hit
CTTTATGGGAAGTCAGAGGACAATGGAATGACATCTTTAAAATACTGAAAGC730.1249400972136647No Hit
ATTTAAGGGCCTCCATGTTCATTAACTTTCCACTGATTTAACAGTTCTAACC720.12322858903265559No Hit
TGACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.12322858903265559No Hit
TGCCTTGGGATATACAAATATATAGACATCGCATGACCACATATTAAGTATG710.12151708085164648No Hit
GGTCTAGGGCATCTTCATCTTAAAACTTCATCTTTTGAAATCTCTGAAAATC710.12151708085164648No Hit
GCTTATGGGATGAAACTACTGGAATAAAACTGGACTAGGCAAAAATTTCTTG710.12151708085164648No Hit
GGCAACGGGACTACAGGTTGAGTATCCCTTATCCGAGAGCCTTGGGACCAGA710.12151708085164648No Hit
TAAAGGGGAACATCATAATGAAGGACAAGCATGATGGCACATTAGATATCAA710.12151708085164648No Hit
TAGTCCGGGCATTGTTGCTACCTATCCTGAATCAAGCCTTGAGCCTAAATCA710.12151708085164648No Hit
ATGGTAGGGTGGTTTTGTCCGTAGAGTATTAAGAATATATCCCTGACTTATA700.11980557267063736No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT700.11980557267063736No Hit
GCTGCAGGGTTATTTTGAGCTTTGCAAGGAAAGTTAAATTCACCATTACCAC700.11980557267063736No Hit
TCAGCGGTGTTGTATTTCTATACAGTAGCTTGAAATTGATTAACTTATACTG690.11809406448962825No Hit
TCTGTCGGGATTTAAAAGAATGGAAAATGACTGAAATCCAATTTAGATTATT690.11809406448962825No Hit
ATGTTAGGGCAAAGGCAATGGATGGGTACCATTTTAGAATGAAAAATTATAG690.11809406448962825No Hit
TGGGATGGGCAGAAGAAGAAAGATCTAAAATCAGTAATCTAAGCTTCTACCT690.11809406448962825No Hit
CATACGGGGGTTCTACAGGTGGATAAATGAGAATAGTACCAAGAATAGAACC690.11809406448962825No Hit
ACTGGAAGAAAGAGTATCAGCGATGGATGATGAAATGAATGAAATGAAGCGA690.11809406448962825No Hit
ACACAGGGGAAATATAAATGTGCATGCACATACACCTTGGTAATTGCTTATG680.11638255630861914No Hit
ACTAAAGGGATCTATTTCTGTGTTATTCTAGCGATGGATTTGTGCCGACAGT680.11638255630861914No Hit
TAGATTGGGGGGCCAGGCCCCAGCATGATCCTGAAATCTGCATCCGCCGTGG680.11638255630861914No Hit
TCTCACGGGATCTGTACTAAATTGACTATAAATTGAGTCTGCAAAGAGGAAA680.11638255630861914No Hit
GACTAGGGGGTCTTGTGTCAAGGCAGGAGGATAACCCGGATGACCTTCTGAG680.11638255630861914No Hit
GGCTCTGGGCCTACTGGTGTGAATATGGAGTCAAATAAAAATCCAGTTTACA680.11638255630861914No Hit
AGTAATGGGAACCTGAAGATGGATTTTTTTTCAACAAGAAGATAGATGGGAG680.11638255630861914No Hit
CGCATTGGGGTATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCA670.11467104812761006No Hit
GTTAATGGGATGTGGAAGATTGTTATTTCATTAGGATTTAGTAAAATTTTTT670.11467104812761006No Hit
ATGCAGGGGATTTCTACCTTCATTTCCAACTTTGTATTGTGCTTTGAAAACC670.11467104812761006No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC670.11467104812761006No Hit
ATGAAGGGGAATTTGTGCAGGAGAAGGACTTGCCCGCATGGAGCTATTTTTA670.11467104812761006No Hit
GGCAACGGGGCCAGCCCTAATAAGAAGCATGCTAAAGCCACAGCAGCTACTG670.11467104812761006No Hit
CAGCATGGGGGTCGTCTACCTGTACACCGTGGTGGCCTTCAACTTCTTCCGC660.11295953994660095No Hit
CGAACTGGGGGACGAATGCCTTTTCCGGTGAACCATGGCGCCAGTTCAGAGG660.11295953994660095No Hit
GATTAAGGGGAAGAAGTGCGGAAGAACAAAGAATCCAAAGACCCTGCTGACG660.11295953994660095No Hit
CTTTTTGGGGGTTTGTGAGTGATCACTCTCAGCATAAGTGGTTGGTTAAAGC660.11295953994660095No Hit
AAAGCTGGGATGCAATTTATGACAAAATTATTTATGATAATTGGATGGCTGC650.11124803176559184No Hit
GCTTGAGGGGCTGTTGCTAAAGGTGGCGCAGAAACAAATATCAGTGTTAGTC650.11124803176559184No Hit
CAAGGCGGCATGTGAGTTGTATGTGCCCCCAGTGCTACATACGCAGGTATGC650.11124803176559184No Hit
TTGACAGGGATACAAGGATCAACATTATTTAAAAATTATACATATTAACTCT650.11124803176559184No Hit
TCTAGTGGGATGTAAAGACCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAA650.11124803176559184No Hit
CTTACCGGGTACATGAAAGCTATTTCATATCTTGGACTCTTTCTTATTCTAG650.11124803176559184No Hit
AAGATAGGGCTAATATGCTTTTTTTCTTTTATATGTTTAAATCTTTGATCTG640.10953652358458273No Hit
TATCCTGGGGACTAAATAATAATTTGAGATATCTATAACAACACTAATGTAA640.10953652358458273No Hit
CATCATGGGAGTACTCTATTCTTCAAATATCCGTTTCTTGGCATCGGGCATG640.10953652358458273No Hit
TACATCCGGGATTGAGTGAGAGTTTCACAGGACCTCTCTGTATTTACAACTT640.10953652358458273No Hit
GTTTGAGGGACTTCTTATGAAGTTGGCATTACCTGAGACTCTTAGCTGTGAT640.10953652358458273No Hit
GAGCAAGGGCTTGGCTATAAGCCATTCTTTGGAAACTCTCCGAGTACCTGAA640.10953652358458273No Hit
GGTGGCCGGGACTAGGGGAATTTTTTTTCTTTATTAACAGATAAGAATTCTG640.10953652358458273No Hit
GTCTCAGGGCTCGTAGGAAATGTATCGGTGTGGGAGGCCTGGGGTACGTGAG640.10953652358458273No Hit
TATTCAGGGTAAGAAATTGTCACAAGAATAAACAGAAAATGAGAAAGCCCTG630.10782501540357362No Hit
TTGTTGGGGATGGTTTCAAAACATTTAGAAGGCACTACTCATGAGTTGCCCC630.10782501540357362No Hit
TCGGATGGGCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGATC630.10782501540357362No Hit
CACTTCGGGTGAGCCCCAGACAAATAATGTTTTAAAGTTATCCCCTTGCTAC630.10782501540357362No Hit
CTTGTTGGGGGAAAATCATGCCTCCAAATTTTTATTTTTCAGCTTAAAAAAT630.10782501540357362No Hit
AACAATGGGATTGCCTAGTGACGTCTTAGCCATTGTAATGTTGTAGCACAAG620.10611350722256453No Hit
CTTCGTACGGGGTGGGGGACGCTGGCGTGCGGGGCACCGCCCAGCAGCCCCG620.10611350722256453No Hit
GCATGCGGGCTCAATCACTTGGTTCATTGTTCAGCAATATGGCCTAAGGGGG620.10611350722256453No Hit
ATAATTGGGAGGTGTCTGGTGAGGGTCCAGTCTCTGGTTTCAAGATGGCACC620.10611350722256453No Hit
AAATATGGGCTTCATTTCCCATTCATGCATTCAAAGACACAGGGTAATGCAC620.10611350722256453No Hit
TTAACTGGGCAGTTATATCAAAAACAGATTCACATTTTGATTGGTGGGAAGG610.10440199904155542No Hit
TTTTTGGGGAGGATTTTAAATTACACAGTTCACAAACAGTAAAGGCCATGTG610.10440199904155542No Hit
ACTTCTGGGAGACATAGAGTGGCACAGTGGATAAAAGATACTCATCAGTACC610.10440199904155542No Hit
CGTATGGGGGTAAATATTTATCAATAAAAATAAACATTTTGTTTTTCTAATA610.10440199904155542No Hit
AGACCTGGGCTGGAATTTTTCATTTCAAGAGTATATTGATCAACCCCCATGC610.10440199904155542No Hit
GGCGCTGGGGATTAGTTTTAAGACAGGACTTTTTAAATTTACAATATTTTTG610.10440199904155542No Hit
GGCGCAGGGGGATTGGAGCCCTGCCAATACCGTGATTTTAGACTTATGAGCT610.10440199904155542No Hit
ACGCTAGGGAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCA610.10440199904155542No Hit
AATTGTGGGCAAAATGAGTACCCTAAAGAACCATGATGAACATGGAATCAAA610.10440199904155542No Hit
CCCAGGTTCTTGAGAGGCTGAGGCAGGAGGATTGGTTGAGCCCAGGTGTTGG600.10269049086054631No Hit
ATTTTTTGGGTCTAGACAGAAATTAACAAAGAAATATTGAATTTAAACTGCA600.10269049086054631No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC600.10269049086054631No Hit
CAACCAGGGATTCACTTAGTTTTCCTTTTTTATTTGAAAAAATACATGACAT600.10269049086054631No Hit
ATCGCTGGGAACTTGAACTACTGAGTCTCCATAGATGTTTCAGACCTGTGTC600.10269049086054631No Hit
TTCATGGGGAGTCAAACAAGTTGTGCCTTTCACAGCTTCTTTACTACTGCAA600.10269049086054631No Hit
TACTCCGGGCTCTGGGAATGGAGTATAACAATCAGATTTGAAAAGGTTTCTT590.1009789826795372No Hit
GGAGTAGGGAAACTTACTACTTGATGGAGTGAGCTATTCTTATGGTAAGCCG590.1009789826795372No Hit
AAAAATGGGCTTCTTTCCCTTTTAGTTAATAATTACACTATTATCAATTACA590.1009789826795372No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCT590.1009789826795372No Hit
GCCTTGGGATATGAATCATGAATCAAGAGGCTTTACTCTTTTTCCCTCAGTT590.1009789826795372No Hit
GCAAAAGGGAAGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTTTGTTG590.1009789826795372No Hit
AAACGCGGGATAGAAAAACATTCTACACAAAAATCTCCCTGTTTTCTATTCA590.1009789826795372No Hit
GAGGTTTATGTTCCAGCTAATGTTATCAAATTTATGCTTCAAACTGTGATAT590.1009789826795372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)