FastQCFastQC Report
Sat 31 Dec 2022
SRR5846231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58316
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGC12942.2189450579600796No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCCGTCT12862.205226695932506No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGT2150.3686809794910488No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTA1660.28465601207215857No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGTATGCC1340.22978256396186297No Hit
ATTTCAGGGAACTCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAA1300.22292338294807598No Hit
TCGTCCGGGATGGCCTACCTAGTGACAATGGATGGTTGTGAGGATTAAACGA1250.21434940668084232No Hit
GCATCAGGGCCAGGCAGGACTACAAATGGCCCAGACCCTTCAGAAATGAAGG1250.21434940668084232No Hit
GAATATGGGCACTTATGTGAAGTCATTTTGAAAACGAGCACTATACAATTAG1040.1783387063584608No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT980.16804993483778036No Hit
CCATAAGGGATGACCCATTTGCCTAACTTTTTTTCAATGTGATGAATGAAAA970.16633513958433363No Hit
TGTACGTGGGGATACAGCTAAGGGAAAAAATAGTGAGCTTGAAGATACGTCT960.1646203443308869No Hit
GCCTAAGGGAGAATTTTACTTCATTGCAGAAAGGGTAGCTGCCTCCTCAAGG950.16290554907744015No Hit
AATTTAGGGACAACATCTTATGTACTCCATAAATGTATACACCTACTATGTA940.1611907538239934No Hit
ATACTAGGGGGGGAAATAGTTTCTTTTAGTTATGTATCCCAACTATCTAATC940.1611907538239934No Hit
ACGCTGGGGCGAATAAAGCTTTAAAAACCAGGATGTAGAAATTGTAAGAATA940.1611907538239934No Hit
TGCATGGGTACACCATTGGCACAGCACTTAGTAAATCTCAATAGTCCTGTTT930.1594759585705467No Hit
ACAGTGGGGCTGATCTCTGGGACACAACTAAAGCATTGTATAGAGGGAAATT920.15776116331709994No Hit
TCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTG920.15776116331709994No Hit
AACTTTGGGGAGCTACGACCGCGCCATCACCGTCTTCTCGCCCGACGGCCAC920.15776116331709994No Hit
ACGAACGGGGCCTGTTTTCTCAACTGTAAAATGGGCTGAACAATGCACTCCT900.15433157281020649No Hit
GCCAATGGGGGAACATTTCCCTGTGATATTTCTGTCCTTGATATTCATCAGG890.15261677755675973No Hit
ACGCGCGGCAAGCACAGCTAAAGAATGAGGACATTTTCATATCAGTGGTTTT890.15261677755675973No Hit
AGCTCGGGGAGGTTCAAGGGTACATGTGCAGGTTTGTTAAATAGATAAACTC890.15261677755675973No Hit
CAAGGCGGCATGTGAGTTGTATGTGCCCCCAGTGCTACATACGCAGGTATGC870.14918718704986625No Hit
CTTTATCCGGGGTTATCTCGAGAAGTATTTTATCTTCTGCATTCTTTCTGTT870.14918718704986625No Hit
GGCTATGGGAGTTCAAAAGATGCTAGACAATGGCGTCCAAGTTCAGCCTTTG870.14918718704986625No Hit
TGACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.14747239179641952No Hit
TATATAGGGGATCTGAAATAGGGGCTTTCATTGATTTTTGTCAAATTTTTTA850.14575759654297277No Hit
AGTAATGGGAAATCAGAATTCATTTAGCTCACCACATCTCTTGAATGTGATT830.14232800603607929No Hit
GTACATGGGAGCAGGTTCCTCTTCTGGAATATTAAAGAGGAAAACGTAGAGG830.14232800603607929No Hit
AGTTGGGGGAGACACACAAGAAATGTGTTATAGGTTCCCAAACATTCTTCAT830.14232800603607929No Hit
TCATTTGGGACTTGAGGTCTTGCTTAATCACTTTTGTTGTCCTGAGGACTTG830.14232800603607929No Hit
CTGCCTGGGGTACCCTGAAATACAGGTTTCTGACAACTTTAAGATCATACAA830.14232800603607929No Hit
CCTACATCAAGAAGGAGAGCCCCCTCCTCAATGATTACCGGGGCTTTCTGGC830.14232800603607929No Hit
TCTGTCGGGATTTAAAAGAATGGAAAATGACTGAAATCCAATTTAGATTATT830.14232800603607929No Hit
GTCAGTGGGCAATATAGTAATGCTTTTAGTAGAGTACTAGATAGTATCAGTT830.14232800603607929No Hit
TAAATGGGGGATTGCTGGGTTGAATAATAATTCTGTTTTAAGCTCTTTAACT820.14061321078263256No Hit
ACTGGAAGAAAGAGTATCAGCGATGGATGATGAAATGAATGAAATGAAGCGA820.14061321078263256No Hit
AAGGCAGGGATGTGGAAATTTAATAATATATTTTTGAGCGACCAATGGGACC820.14061321078263256No Hit
ATACAAGGGCAGGAAATCAAAGACCTAAAGACAGAAATACCATTCAACTCAG810.1388984155291858No Hit
ATGACTGGGACATTCATGTGATGATGCTCAATTGCTTATGTCAGTGGAATGT800.13718362027573908No Hit
ACATGGGGCCTGTGAGTATGTGTGGGGGACAGCTGATAGAAGGGAGGAGAGT790.13546882502229235No Hit
CTTGTTGGGGGAAAATCATGCCTCCAAATTTTTATTTTTCAGCTTAAAAAAT790.13546882502229235No Hit
ACATGAGGGATTCAGCCATAAGCTAACTTTCCTACAAGTATGAAGGGCATGG780.1337540297688456No Hit
GTTAATGGGATGTGGAAGATTGTTATTTCATTAGGATTTAGTAAAATTTTTT780.1337540297688456No Hit
CTTTATGGGAAGTCAGAGGACAATGGAATGACATCTTTAAAATACTGAAAGC780.1337540297688456No Hit
CATACAGGGCCGTCAGTGATGGCCCCTTCAACAATAGCACTGGGATTCCTTT780.1337540297688456No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAT770.13203923451539887No Hit
TGCTTAGGGTGGCACAGCTTTAGTGGAACACCCCATGGGTGGGAGTGTACAC770.13203923451539887No Hit
TCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCC760.1303244392619521No Hit
ACACAGGGGAAATATAAATGTGCATGCACATACACCTTGGTAATTGCTTATG760.1303244392619521No Hit
GGCTCTGGGCCTACTGGTGTGAATATGGAGTCAAATAAAAATCCAGTTTACA760.1303244392619521No Hit
ATTGAAGGGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAAAAGAGAAAAAAG750.12860964400850539No Hit
AAATATGGGCTTCATTTCCCATTCATGCATTCAAAGACACAGGGTAATGCAC750.12860964400850539No Hit
CTTAGAGGGGGTGGTTTCAATGGCTAATTAGAATGTGTTATTTGATTTCTGC740.12689484875505866No Hit
TGATAAGGGCAAGCCAAATGTCCAACAATGATAGACTGGATTAAGAAAATGT730.1251800535016119No Hit
ATCTACGGGAATGAGCCTAAACTGACTCCGTTGGGCCAACACCTTGCAGCTT730.1251800535016119No Hit
GCCTTGGGATATGAATCATGAATCAAGAGGCTTTACTCTTTTTCCCTCAGTT720.12346525824816516No Hit
TTGCTAGGGCCTGAGGTGATCGGTGTCTTTGGTGAGTCTGTTAAAGGCTATG720.12346525824816516No Hit
CAGTCGGGGGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTT710.12175046299471844No Hit
TGGACAGGGAGAGAAGCAACACCAGAAAATGATCCTAACTACTTTATGAATG710.12175046299471844No Hit
TTGACAGGGATACAAGGATCAACATTATTTAAAAATTATACATATTAACTCT710.12175046299471844No Hit
TGCCTTGGGATATACAAATATATAGACATCGCATGACCACATATTAAGTATG700.1200356677412717No Hit
GGTCTAGGGCATCTTCATCTTAAAACTTCATCTTTTGAAATCTCTGAAAATC700.1200356677412717No Hit
ATGCAGGGGATTTCTACCTTCATTTCCAACTTTGTATTGTGCTTTGAAAACC700.1200356677412717No Hit
ATTTAGGAAGAGAGCAACAAATTTGATTATATTTTTAAGTGTCTTAGATCCT700.1200356677412717No Hit
TCTCCTGGGATAGTAAGTGACTCACAAAAAATCAGTGCTTACTATTCATCTG700.1200356677412717No Hit
AAGAAGGGGCATCATGCTAGGACTACTATTCCTAAATGGCTTCAAAGAGGAG690.11832087248782495No Hit
ACATGGGATACCTTACCCTTAGACATGTAATCTTAAAAAGTTGAGAACTGCC690.11832087248782495No Hit
TCATAGGGGGTAACTGTACTCTTAGATATGGTGTTAGCAGTCAAGAAGCCGG690.11832087248782495No Hit
TTGGTCTGGGTATCCCAGATGTTTATTTATTTTCATTTTCAAACAGAACTTT680.11660607723437823No Hit
TCTAGTGGGATGTAAAGACCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAA680.11660607723437823No Hit
AGCGATGGGACTTTAAGGAAACAAACAAGATTACAACAATTAAAAAAACACA670.11489128198093149No Hit
AATGGCGGGCTCTAGGATCTGCATATAAACAATAAATGTTTCCTAGAAATTA660.11317648672748473No Hit
TCATATGGGAAGTGATGCCTGGACTTACTGCATCAGTAAAAATGAATGGATA660.11317648672748473No Hit
CGCTTAGGGAAGAATATGTATGTAAAGACATTTTGGAATTTCCTGGATGAGA660.11317648672748473No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTAACTCGTATGC660.11317648672748473No Hit
GTTTGAGGGACTTCTTATGAAGTTGGCATTACCTGAGACTCTTAGCTGTGAT660.11317648672748473No Hit
TTCAGGGCTGGGCCCAGATTGTCTGGTTGGCAAGAGCAAAGTTTCCGTTGAT660.11317648672748473No Hit
GCCAAGAGGGAACACTCCATTTCTCATTTTAAAAATGAGGAAATGAAGTATA660.11317648672748473No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCTCGT660.11317648672748473No Hit
AACTTAGGGATAGGGCAGACAGTGTAAACATGGCCTTACCTCCTAATGCTTA660.11317648672748473No Hit
CGATAGGGACCATGCTAACAGTAAGCATAAGAAAGCTGCAGTAGCTATAGTA660.11317648672748473No Hit
GCTGCAGGGTTATTTTGAGCTTTGCAAGGAAAGTTAAATTCACCATTACCAC660.11317648672748473No Hit
AATTGTGGGCAAAATGAGTACCCTAAAGAACCATGATGAACATGGAATCAAA660.11317648672748473No Hit
CGTATGGGGGTAAATATTTATCAATAAAAATAAACATTTTGTTTTTCTAATA650.11146169147403799No Hit
GGCAACGGGGCCAGCCCTAATAAGAAGCATGCTAAAGCCACAGCAGCTACTG650.11146169147403799No Hit
AGTCTAGGGCTTTCTGGCATGGATCAAGAGAAAAGAAAATACAAGAAAACAG640.10974689622059126No Hit
GCTTATGGGATGAAACTACTGGAATAAAACTGGACTAGGCAAAAATTTCTTG640.10974689622059126No Hit
CACACTGGCTTATATTCATGGGATAGAAACATAAAGGATATCTAGCAGGTAT640.10974689622059126No Hit
CTAGCTGGGCGATTTTTCTCTGTCAAGCTTGAACTAATGTAAATAATTGAAA640.10974689622059126No Hit
TCCAAAGGGTGCTTCCTGAACAGTATTAAATTATATACTTAATTTTGACTAA640.10974689622059126No Hit
CGAATAGGGACTCTTTAGAAGATGTGGAGAGACCCATAGTTTAGAATTTTCT630.10803210096714452No Hit
TTGTTGGGGATGGTTTCAAAACATTTAGAAGGCACTACTCATGAGTTGCCCC630.10803210096714452No Hit
TTGACAGGGAGATTAGGGTTCTCTGGGCATAAAACACATAGATGACAACAGG630.10803210096714452No Hit
TCGCTGGGGGCACGTTAAGCCATGCTGTAAGCATTGTTTTTATTTTCAGGCT630.10803210096714452No Hit
CTTACCGGGTACATGAAAGCTATTTCATATCTTGGACTCTTTCTTATTCTAG630.10803210096714452No Hit
AAGATAGGGCTAATATGCTTTTTTTCTTTTATATGTTTAAATCTTTGATCTG620.10631730571369778No Hit
GTGAATGGGAGTTTCTTGTTAATGTATGTTTGAGGAGTTTGAACGTCAGTGT620.10631730571369778No Hit
TCTAACGGGACTGACCATGGTGTACCCAAAATGATTTCAACTAATACGAATT620.10631730571369778No Hit
GCTCAAGGGCTGGCCTTTGCAAAGCACGTCTGAAGAAATGAGCACTTTCTAT620.10631730571369778No Hit
GATTAAGGGGAAGAAGTGCGGAAGAACAAAGAATCCAAAGACCCTGCTGACG620.10631730571369778No Hit
TACATCCGGGATTGAGTGAGAGTTTCACAGGACCTCTCTGTATTTACAACTT620.10631730571369778No Hit
AAGCACGGGATACCCTCTTTTCAACTGTCATATATTTGACCTTCACTAGATT620.10631730571369778No Hit
GGTACTGGGCCTCACAAACTTCTTTACGAAGAGTGAAATATGTTTTTGTACC620.10631730571369778No Hit
CCCGATGCGACCCAGTTTATAGAGTTTGCCAAACTTTCTGATTTTGCAGATG620.10631730571369778No Hit
AGCTTTGGGTGCCATTCCCTTATCTCGACTAATATTATAGAGAAACTGTCTT620.10631730571369778No Hit
GTAACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.10460251046025104No Hit
ATTTTTTGGGTCTAGACAGAAATTAACAAAGAAATATTGAATTTAAACTGCA610.10460251046025104No Hit
TGCAAAGGGACTGTGAATGTTGTATGGAATTGATAGAACAATTTCTTTTTTA610.10460251046025104No Hit
CAAGGGGGAAGAGTGTAAATAACATTTGCATTTTATAATAATGTAATCCTGA610.10460251046025104No Hit
TCAGGCGGGCCAGTGAGGGTGCATTTGAACACTGATTATATTCTCCATTTGT610.10460251046025104No Hit
GCAAAAGGGAAGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTTTGTTG610.10460251046025104No Hit
GGCGCTGGGGATTAGTTTTAAGACAGGACTTTTTAAATTTACAATATTTTTG610.10460251046025104No Hit
GTTAAAGGGAAGATTTGCATTAGTTTTTCTCCTGCACCCTTAAAAGTGATTT610.10460251046025104No Hit
TACACAGGGAACTTCAGAAATCTTAATTTACCAGTGAATTGTAATGTTGTTT610.10460251046025104No Hit
CTTTGGGGGATTATTTTGGCCTGAATGCTGTCATATATGTAACATGTTAGTA610.10460251046025104No Hit
GTAACAGGGCAACCAATTTTGCCATGAAAAGGGATGTCCAGGAAGGTCAGGC610.10460251046025104No Hit
AGTAATGGGAACCTGAAGATGGATTTTTTTTCAACAAGAAGATAGATGGGAG610.10460251046025104No Hit
TTAACTGGGCAGTTATATCAAAAACAGATTCACATTTTGATTGGTGGGAAGG600.10288771520680431No Hit
TTTTTGGGGAGGATTTTAAATTACACAGTTCACAAACAGTAAAGGCCATGTG600.10288771520680431No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTATCGTATCCTGTCTCTTATAC600.10288771520680431No Hit
GGTGGCCGGGACTAGGGGAATTTTTTTTCTTTATTAACAGATAAGAATTCTG600.10288771520680431No Hit
AATGGTGGGAGAAGCACATGGGAAATGAAATTTTATGTCTAATTAGATATTT600.10288771520680431No Hit
TTCGCAGGGAAGAGCAGGGTGCTACATTGGGTGCTCCTGTTCTCGCAGAGTT600.10288771520680431No Hit
TAAAACGGGTGTTTCTTCTCCACTCAAAGTTACTCATTCCCTGTAGCTGAAG590.10117291995335757No Hit
TGATATAGGATACATTGTGAATCTCTAAATCCACGGAAATGATATAAGAAAA590.10117291995335757No Hit
ATTTAAGGGCCTCCATGTTCATTAACTTTCCACTGATTTAACAGTTCTAACC590.10117291995335757No Hit
GGCAACGGGACTACAGGTTGAGTATCCCTTATCCGAGAGCCTTGGGACCAGA590.10117291995335757No Hit
TGGGATGGGCAGAAGAAGAAAGATCTAAAATCAGTAATCTAAGCTTCTACCT590.10117291995335757No Hit
GAGCAAGGGCTTGGCTATAAGCCATTCTTTGGAAACTCTCCGAGTACCTGAA590.10117291995335757No Hit
GACTAGGGGGTCTTGTGTCAAGGCAGGAGGATAACCCGGATGACCTTCTGAG590.10117291995335757No Hit
TTTTTTGGGATGTGCATCTTTTGTCGAATGTTTTGGTTCAAGAAAGAATGTT590.10117291995335757No Hit
GGCGCAGGGGGATTGGAGCCCTGCCAATACCGTGATTTTAGACTTATGAGCT590.10117291995335757No Hit
TGGATTGGGCGATCTGATTTAGGGCAGATGCAAAATCACCCCTTCTGCAAGA590.10117291995335757No Hit
GAATATGGGCTGAAGCGGGGATCTTGTCCCACTGAATTGCCCTGGAATTAAT590.10117291995335757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)