FastQCFastQC Report
Sat 31 Dec 2022
SRR5846222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133754
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC4360.32597155972905484No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT4100.3065328887360378Illumina PCR Primer Index 8 (95% over 21bp)
CTCTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.11663202595810218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)