FastQCFastQC Report
Sat 31 Dec 2022
SRR5846209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107024
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC3190.2980639856480789No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT2800.2616235610704141Illumina PCR Primer Index 8 (95% over 21bp)
GCAGCGGGCCCCTAAGGAATAAGAATTATACATAAAGACGATTCTCAGCATC1520.14202421886679623No Hit
ACTAGGGGGTGGCAGCCCGGCGCCATCTTCATCGAGCGCCATGGCCGCAGCC1380.12894304081327554No Hit
GAATCTGGGATAAAGACACCACTCAAAAGGAAACTTGAATAATTTATAATTT1370.12800867095230975No Hit
GAGTAAGGGATGTAGAAAATTCCAAAGAATCAACAGAAAAACTCCTGGAACT1340.12520556136941247No Hit
TATAAAGGGGGCCACAAAACACCAAAGAATTGTAGGCAGTGGCCCCTATTGA1340.12520556136941247No Hit
GAAATAGGGAATCACCACAGGACTATTCCTAGCCATGCACTACTCACCAGAC1330.1242711915084467No Hit
TAATCGGGGTACCAAATCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTA1330.1242711915084467No Hit
TTAGTAGGGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACA1310.12240245178651518No Hit
TCAGTGGGGCGATCTTGCGGTGGGATAAAGAAGATAGAAAAAAAAGACTTAG1300.1214680819255494No Hit
GGGCATGGGAAACCATACCCTGAAGTCCAGTCATTGACAAATCTTCCCGCTG1300.1214680819255494No Hit
ATGGCGATCTTTCTGCGGGTTTTGGAACCTGGTATGGTATTTCAGACGTCTT1300.1214680819255494No Hit
GACTTGGGGAGATTCTGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTT1280.11959934220361788No Hit
GAAGTCGGGTAATCACTTGTGGAGCGCCAGCTCTGCCCAAGACGCCACCTGG1270.11866497234265211No Hit
GTGGCAGGGTTACCAGCTCAACTAAAACCTGCAATTCTGCATTTCAACTCTT1260.11773060248168636No Hit
TGGCTTGGGAATGCACAGTGAGGAAGACAGGTCAACTCAGTTGTGAACTACC1260.11773060248168636No Hit
TCAAACGGGAGCGCTGGGGCCGTGGAGCCTTTGGTCACCTGCGCCTCTTCCA1230.11492749289878906No Hit
AACTTAGGGAGTGTGCTTGGGCCAGCTCTCCTGCCTGTGTCTAGAGGAAGCC1230.11492749289878906No Hit
AACGCTGGGAACTGCCATGGACTGGTTTATTGCTGTTTGTTATGCATTTGTG1230.11492749289878906No Hit
CGATGGGGGAACATTATGAAGGTGGTTTTGAAGATAATGCTAGAATTTATGA1220.11399312303782329No Hit
TTAAACGGGTGATGCACTGGGTGTGGAGCTGAGCAGAGAGGCCTAACCAGGA1220.11399312303782329No Hit
GGGCCAGGGGAAAGAAGCATGAAGAAAACAGCTGCATTTGAAGAGAAACGGA1210.11305875317685753No Hit
GCCGTTGGGGAAGGAAATGTTTCACCTTATTTTTGGTCTTTGTTTTATTCAT1210.11305875317685753No Hit
ATTGCCGGGCAGCCCCTCTCGGCCCCAGCCGCATCATCCCACCTGTCCCCTC1210.11305875317685753No Hit
ACTATAGGGACCATGAGGCAGGCGACCTTGGCTATCAGACTTGAAATATTCA1200.11212438331589175No Hit
CTAGTTGGGCGATCTAACGGTGGCTGTGAAAGGAAAACAAAATATTTGTCCA1190.111190013454926No Hit
AGTTATGGGCACATAATGAGCCGTTACAACTCCAGCACCAAAGCTAACGCCC1190.111190013454926No Hit
GACCTGGGGAGAGGTGCCCCTTCCTCAGCTGGGAGTGGGCCGCCTCATGCTC1190.111190013454926No Hit
CCACCCGGGACAGACCTGGCCTACACGGATATGGGGAGCCACTGAGGGATGT1190.111190013454926No Hit
CAGCCCGGGCAAAGGGGAGCCCCCAGCACCCAAGTCACCGCCAAAGGCCCGG1180.11025564359396023No Hit
TTATGGGGATGCAAAGTAATAGTTTTTACCTTTTTAAAAAATACATTACTTA1180.11025564359396023No Hit
TTTAGTGGGGGGGAGCGTGGGGTATCTCGAGGTGCCGGGTTGCAGGCGCTCA1180.11025564359396023No Hit
CACAGATGGTTTGGCCCTGCAGTGACCAGAGCAGCCCAAGCCGCCACCATGG1180.11025564359396023No Hit
AGCTTAGGGGGGGGCGGCGCGGGGCGCGGACACTCCCCGCTGAGAGTCCGCC1180.11025564359396023No Hit
AAGTCGGGGGTGATCCTTTAGAACCAACTCAGACACCAGATCCACTGGAAGA1170.10932127373299445No Hit
GCCAATGGGAAAGTGCTGGGACTATAGGAATGAGCCACCGCACCCAGCCTGG1170.10932127373299445No Hit
GAATTAGGGCATCAGCTGAGCCTCAGAACTCATCTACCTGAAAAAGTGATAA1160.1083869038720287No Hit
GAACAAGGGATGCCAGTTTCTAATGTTGGATTTGATGTAGACAGTTTGGAAT1160.1083869038720287No Hit
GAGTAAGGGTGGCAAGGGACAATTATAGATTTCAGTATATTTTCTCAGAAAA1160.1083869038720287No Hit
TGTTATGGGCCTGCCATCTCCCTGGCTTATGAGCAGGCTGAGAGTGACATCA1160.1083869038720287No Hit
CCGCACGGGAGACATTGTGAAAAAAAGGAAGCCATAAAGTGGATGAAATTAT1150.10745253401106293No Hit
TCTCCTGGGGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACT1150.10745253401106293No Hit
ACAACCGGGATATTAAGGCTTACTAGTATCACTGCTTTTTCCTTAGCTTAAT1150.10745253401106293No Hit
TTACTAGGGATCCAGAGACCCTGTTTAAGAGCATCCCCCAGTCCTTCTGGTG1150.10745253401106293No Hit
ACACATGGGGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAG1150.10745253401106293No Hit
AAAGCGGGGATTTCTACCCAGGGACAAATGAGGAATATAACTAGCAGAGATT1150.10745253401106293No Hit
ACTTGCGGGATATTTTGGAGGACGGGTGAAGAGGTAATAACGAAAGCAAGCG1150.10745253401106293No Hit
ATTGCTGTGAATTGCACTGTGTGGCACCCCACAGTGGCTGTCTGCCCAGCAT1150.10745253401106293No Hit
GTAAATGGGAAATTCTCAAAGCTACTTCTCCTAAAATGCTACATTGAAAAAG1140.10651816415009718No Hit
AAAGCTGGGAAAAGTTATTCTCCTGGGCCCTTGAGGCCAAGGTAGATTCAGA1140.10651816415009718No Hit
CTGTACGGGCCTGGTTATCCAAGGATAGCAGAAATGTGTTTCTGTGGCTGAT1140.10651816415009718No Hit
GTTATTGGGACAGATTTGGACATGTATTGCTTTCATTATCATTCAGTATTTT1140.10651816415009718No Hit
GCGTAGGGAGCTGCGGGATGAAGAAGAAAAAATAGAAGAAAGAGAGAATGGG1140.10651816415009718No Hit
GTTGCAGGGTCTTAAAAAAAGGCCTCCGATGAAGCAGAGGAAAGTGGATCAC1140.10651816415009718No Hit
GAATCCGGGGCTTGAAAGTATAAACCATCACTTAATTCTTATTTTAACTCTC1140.10651816415009718No Hit
AGCACGGGTACAAGGGATGTCAAGGACCTCTTCAAGGAGAAGTACAAACCAC1130.10558379428913141No Hit
AATGATGGGATTGCAACAGTCCTCTGAATGTGACTGAATAAAGTTCATATTG1130.10558379428913141No Hit
CGCCTTGGGGTGCCCAGAACAGAAAAAGATACCAACCTTCATTTAGTTTATG1130.10558379428913141No Hit
GGAAAGGGATTGCTGTAACTCTTTTTTGTCACCTGATATGGTCAGCTTTGTA1120.10464942442816563No Hit
GCGCAGGGGTCTACTCGTGGGGGATCTCGATATTTCAGATGGGCTTTTGATG1120.10464942442816563No Hit
AAACTTGGGGTTTTAAGGGGACAGGCACATTGCAGTTGTAGGTGTTCATTTT1120.10464942442816563No Hit
AACCGGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAG1120.10464942442816563No Hit
TGCTGGGGGCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCA1120.10464942442816563No Hit
CTTACCGGGGGCTGTCTTCGAATAGAGTTCTGTTGGGACTGGGACATTTCTA1120.10464942442816563No Hit
TCAGTGGGACCTGCTCCAGATGCTGGACACACTGGTCTTCTCTAGCCTCCTG1120.10464942442816563No Hit
GTAAATGGGGAACTTACCTGATATTTTTCAAAACACATTTTTAGAATTGTTT1120.10464942442816563No Hit
CGTCTAGGGGGGGCAAATGGCTGAATCCTTGTGTGATATTTTTTTCTTCGCT1120.10464942442816563No Hit
CGTAAAGGGTATTAGAGATCACTTTCTATGGCTGACAAATGTCATTTAAAAT1120.10464942442816563No Hit
GATGCTGGCGAAGGAGTCTCATGCTTTCTTCACAAGAGAGTTCACAGGATTA1110.10371505456719989No Hit
TAAGTAGGGCAAACTGGGAGATTTTGGATGTTCAGTAAAGCTCAAAAACAAT1110.10371505456719989No Hit
GTCCAGGGGTCGAGCATTAAAGGGCAGTATTTAAAGTCAGTTGGCAAGCAGT1110.10371505456719989No Hit
TTGCGGGGCTGAGCTTGATTCTAAAGGGGACAGATCTTGAGCAAGGCAAGAA1110.10371505456719989No Hit
GGGTCAGGGCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAG1110.10371505456719989No Hit
AGACTTGGGTAAATACCGGATCAGAAAAGAATCATCAACGGATGCTAAATTT1110.10371505456719989No Hit
ATCGTGGGGCATAGAGTCACTGGCATGATGCCCTCGCTCCCCCATGCCCCCA1110.10371505456719989No Hit
CCAATCGGGGATGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTA1110.10371505456719989No Hit
TGTAGCGGGTGAGAAAGAAATTTTACATTGCAACCCAGTGTGCGTGTACACA1110.10371505456719989No Hit
GTCTAAGGGAACAGCCTTTTTGGTAGTCACATAGCAGAATGATCAGAGTTAC1110.10371505456719989No Hit
TCATATGGAGGAGGAGATTGGGGACCTGACGTTCACTGTGGCCCAAAAGATG1110.10371505456719989No Hit
GTAAATCGGGAGAGGAACAATTCCTGGACATATAATTGGGTAATGCAAATTT1100.10278068470623411No Hit
TTGCCCGGGTGCTTTTGAAAGCTTCATCACTGAAAATGTAATCAAACTTAGC1100.10278068470623411No Hit
GTAAGCGGGCTTATTCACTCTTTATTTGCTGCTGTTTAAGTTGCCAACCTCC1100.10278068470623411No Hit
CGCGTAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1090.10184631484526836No Hit
ATTTGCGGGGTGGCTGTGGAATGATAAGGTCCTAGAGGGGCCCTGGCATTTC1090.10184631484526836No Hit
TGGGACCGGGAAAATAACAAGGTCAGAAAACCTCCATGTTTCTCATATCTTG1090.10184631484526836No Hit
ACTCACGGGGGTAAATGCTTTTAATCAAGCAGGAAAAAGTTCTCATGATTAT1090.10184631484526836No Hit
AGCTTGGGATGGGTCAAGCTTTACAAAAGCGAAAACCTCGACAATCCAATCC1090.10184631484526836No Hit
TCTAGTGGGGTTACATCTTAATTCCATCCAAAGAAGATACAGTTTGAAGACA1080.10091194498430259No Hit
GAAGGACGGGATCTATTTGCAGTAACCACATTGCTGCTGTTTCTTGTATGCT1080.10091194498430259No Hit
TGTACGGGGTAATCACTCATTTTTAATCTACCACAATAAGTATGTGGCAAAC1080.10091194498430259No Hit
AAAGCTGGGGCAGTTGTGAAAGCACCATCATCATAGAGGATGATGTAATTAA1080.10091194498430259No Hit
ATGAATGGGATTACCAAACAAGAGTATTTTTATGAAAGAAAAGGACAAACCT1080.10091194498430259No Hit
GACGCTGGGTAATAAATGGGGGATAATTTTTTTTTTGTAAATCCGTGCTTGT1080.10091194498430259No Hit
TTTGCCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1080.10091194498430259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)