FastQCFastQC Report
Sat 31 Dec 2022
SRR5846208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107288
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC3670.3420699425844456No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT2800.26097979270747895Illumina PCR Primer Index 8 (95% over 21bp)
GAGTAAGGGATGTAGAAAATTCCAAAGAATCAACAGAAAAACTCCTGGAACT1630.1519275221832824No Hit
ATTGCCGGGCAGCCCCTCTCGGCCCCAGCCGCATCATCCCACCTGTCCCCTC1440.13421817910670347No Hit
ATCGTGGGGCATAGAGTCACTGGCATGATGCCCTCGCTCCCCCATGCCCCCA1430.13328610841846247No Hit
AAGTCGGGGGTGATCCTTTAGAACCAACTCAGACACCAGATCCACTGGAAGA1420.13235403773022147No Hit
CACAGATGGTTTGGCCCTGCAGTGACCAGAGCAGCCCAAGCCGCCACCATGG1390.12955782566549848No Hit
GAATCTGGGATAAAGACACCACTCAAAAGGAAACTTGAATAATTTATAATTT1370.12769368428901648No Hit
ACTTGCGGGATATTTTGGAGGACGGGTGAAGAGGTAATAACGAAAGCAAGCG1370.12769368428901648No Hit
ATGGCGATCTTTCTGCGGGTTTTGGAACCTGGTATGGTATTTCAGACGTCTT1360.12676161360077548No Hit
GTAAGCGGGCTTATTCACTCTTTATTTGCTGCTGTTTAAGTTGCCAACCTCC1360.12676161360077548No Hit
TCTCCTGGGGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACT1350.12582954291253448No Hit
TCAGTGGGACCTGCTCCAGATGCTGGACACACTGGTCTTCTCTAGCCTCCTG1350.12582954291253448No Hit
TGTACGGGGTAATCACTCATTTTTAATCTACCACAATAAGTATGTGGCAAAC1330.12396540153605248No Hit
TTAGTAGGGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACA1320.1230333308478115No Hit
GCAGCGGGCCCCTAAGGAATAAGAATTATACATAAAGACGATTCTCAGCATC1320.1230333308478115No Hit
ACTAGGGGGTGGCAGCCCGGCGCCATCTTCATCGAGCGCCATGGCCGCAGCC1320.1230333308478115No Hit
CAAACTGGGGAACAGGCCTCTCTCTGCTGGCCGGCTTCGGAGGCTTCTTGGA1320.1230333308478115No Hit
TATAAAGGGGGCCACAAAACACCAAAGAATTGTAGGCAGTGGCCCCTATTGA1320.1230333308478115No Hit
TGAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1310.1221012601595705No Hit
AGCTTAGGGGGGGGCGGCGCGGGGCGCGGACACTCCCCGCTGAGAGTCCGCC1310.1221012601595705No Hit
TGGCTTGGGAATGCACAGTGAGGAAGACAGGTCAACTCAGTTGTGAACTACC1310.1221012601595705No Hit
CACATCGGGCAGTGGGTCCATGCGCCTGACACTCCTCTCTGCTTGTGGACCT1300.1211691894713295No Hit
ACTCCTGGGAACTCTGGGATTTTAGAAGGTTAGAATATTTTTTCAAGAGAAC1290.1202371187830885No Hit
GACTTGGGGAGATTCTGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTT1280.1193050480948475No Hit
GCGTAGGGAGCTGCGGGATGAAGAAGAAAAAATAGAAGAAAGAGAGAATGGG1280.1193050480948475No Hit
GGGCCAGGGGAAAGAAGCATGAAGAAAACAGCTGCATTTGAAGAGAAACGGA1270.11837297740660653No Hit
TTTGCCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1270.11837297740660653No Hit
CCGCACGGGAGACATTGTGAAAAAAAGGAAGCCATAAAGTGGATGAAATTAT1260.11744090671836553No Hit
GGGCATGGGAAACCATACCCTGAAGTCCAGTCATTGACAAATCTTCCCGCTG1260.11744090671836553No Hit
AAACTTGGGGTTTTAAGGGGACAGGCACATTGCAGTTGTAGGTGTTCATTTT1260.11744090671836553No Hit
GAAATAGGGAATCACCACAGGACTATTCCTAGCCATGCACTACTCACCAGAC1250.11650883603012453No Hit
CTGTACGGGCCTGGTTATCCAAGGATAGCAGAAATGTGTTTCTGTGGCTGAT1240.11557676534188353No Hit
GTAAATGGGGAACTTACCTGATATTTTTCAAAACACATTTTTAGAATTGTTT1240.11557676534188353No Hit
TAAGTAGGGCAAACTGGGAGATTTTGGATGTTCAGTAAAGCTCAAAAACAAT1220.11371262396540154No Hit
TCAAACGGGAGCGCTGGGGCCGTGGAGCCTTTGGTCACCTGCGCCTCTTCCA1220.11371262396540154No Hit
TGACTAGGGGGTGATCAGAGAAATGCAAATTAAAACTACAATGAGATACCAT1220.11371262396540154No Hit
CTCACTGGGAAATACTATAATCACAGAGCTTGCAGAAGCTGCCTGGAGGACC1220.11371262396540154No Hit
TGTTATGGGCCTGCCATCTCCCTGGCTTATGAGCAGGCTGAGAGTGACATCA1210.11278055327716055No Hit
TAATCGGGGTACCAAATCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTA1200.11184848258891955No Hit
CAACTAGGGATTGTAAAGGAATTTGAAGATAGAGGATTTGTGCAGGTGGATG1200.11184848258891955No Hit
GGGTCAGGGCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAG1190.11091641190067855No Hit
AATAAAGGGACTCCATGGGGAATAATTCTGATACTCTAATTAAGAGCAATAA1190.11091641190067855No Hit
GTATATGGGAACAAAGGAATTAAAATGGGATACTAAACAAAAATATTTGACA1190.11091641190067855No Hit
GTAGTAGGGCTGTTGCTTTATGTTGTACATGCCCCATAGCCATCCCAACGTC1190.11091641190067855No Hit
TTGTAGGGCACTTGCCAAACTTAACTTATTTAAACTTAATACCAAACCCATA1180.10998434121243755No Hit
CCAATCGGGGATGCCAACACAGCAGCCATTCAAGCAATCCTATACAACCGTA1180.10998434121243755No Hit
GTAAATCGGGAGAGGAACAATTCCTGGACATATAATTGGGTAATGCAAATTT1170.10905227052419655No Hit
AAAGCGGGGATTTCTACCCAGGGACAAATGAGGAATATAACTAGCAGAGATT1170.10905227052419655No Hit
ATTGCTGTGAATTGCACTGTGTGGCACCCCACAGTGGCTGTCTGCCCAGCAT1170.10905227052419655No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA1160.10812019983595555No Hit
TGGCCAAAGAAATCATTCCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTT1160.10812019983595555No Hit
GAGTAAGGGTGGCAAGGGACAATTATAGATTTCAGTATATTTTCTCAGAAAA1160.10812019983595555No Hit
GTAAATGGGAAATTCTCAAAGCTACTTCTCCTAAAATGCTACATTGAAAAAG1150.10718812914771456No Hit
AGGTTTGGGTACATACATAAATAGAAAACCTGGCAACTTACTAATGTAACTT1150.10718812914771456No Hit
GCAGGGCACCCGGGCAGGCCTCAGAACGGTGAGGGGCCAGGGCAAAGGGTGT1150.10718812914771456No Hit
GTTATTGGGACAGATTTGGACATGTATTGCTTTCATTATCATTCAGTATTTT1150.10718812914771456No Hit
TTATCAGGGGAAAAAAAAGGAAAGAAAAAATCTGTAGGTCGTCCACCTGGCC1150.10718812914771456No Hit
TCTCTCTGGGAGGCAGACTTTGTCTTCTCTTTAGTATACATTTCTGGAAACC1140.10625605845947356No Hit
GCAGTGGGTGACTGAAACTTAAACCCAAAGGCAAGCCCAGATTGTCTGGCCG1140.10625605845947356No Hit
TTGCGGGGCTGAGCTTGATTCTAAAGGGGACAGATCTTGAGCAAGGCAAGAA1140.10625605845947356No Hit
GCTATTGGGGGGTGATTACACTGTCAAAACTTCTAATTCTCAAATATAATCT1140.10625605845947356No Hit
GAAGTCGGGTAATCACTTGTGGAGCGCCAGCTCTGCCCAAGACGCCACCTGG1140.10625605845947356No Hit
AAAGCAGGGAAGAGCAAAACCAAAACTAGGAGCAGAAGTAGAGGTCGAGCTA1140.10625605845947356No Hit
ACACATGGGGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAG1140.10625605845947356No Hit
AATGAGGGGGGCGGAGGCCGAGCACTCGGCGCTGAACCCGCTGCGCGCGGTG1130.10532398777123256No Hit
TAGCCGGGATTGGCATTCCTTGACTAAAAAGGACCACCTGATGCTCAGTCCC1130.10532398777123256No Hit
TGCTGGGGGCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCA1130.10532398777123256No Hit
CGCCTGGGATTATGGAGAATTGGGGCACCCACAGGAATGAAGAGAGAAAGCA1130.10532398777123256No Hit
CTAGTTGGGCGATCTAACGGTGGCTGTGAAAGGAAAACAAAATATTTGTCCA1130.10532398777123256No Hit
TCATTAGGGGCTGAAAAGAACACGAGACAGACAACAGCCTCTCCCATGTTAC1130.10532398777123256No Hit
ACACTCGGGACTTAACCAATATTATTGATTACCAGACTTTTATGAAAGCCAA1120.10439191708299157No Hit
CTAGTCGGGATCTTCTGCCACTGTAATAAAATAGATGCAATTGAGGTTCATA1120.10439191708299157No Hit
TTGGGGACTGAGGCAACTGGGATTGGAAACCTAGTTTTTTTCCTCCAGGAAA1120.10439191708299157No Hit
TAAAAAGGGGCAAGAATGGCTTTGATTGGACTTGGTGTGAGTCATCCTGTTC1120.10439191708299157No Hit
GTCTAAGGGAACAGCCTTTTTGGTAGTCACATAGCAGAATGATCAGAGTTAC1120.10439191708299157No Hit
TCAGTGGGGCGATCTTGCGGTGGGATAAAGAAGATAGAAAAAAAAGACTTAG1110.10345984639475057No Hit
TTTTAAGGGCCAGTGATACTTTGCAGAATTGCACATTAGAACTAGCTACACT1110.10345984639475057No Hit
GGCATTGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGT1110.10345984639475057No Hit
CTTACCGGGGGCTGTCTTCGAATAGAGTTCTGTTGGGACTGGGACATTTCTA1110.10345984639475057No Hit
TTTAGTGGGGGGGAGCGTGGGGTATCTCGAGGTGCCGGGTTGCAGGCGCTCA1110.10345984639475057No Hit
GTTGCAGGGTCTTAAAAAAAGGCCTCCGATGAAGCAGAGGAAAGTGGATCAC1110.10345984639475057No Hit
AATACCGGGATAGTGAGCTTTGATTGCACGGGTACACTCTAGCCTGGGTGAC1110.10345984639475057No Hit
TGTCTAGGGATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGAT1110.10345984639475057No Hit
CTACTTGGGAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGA1100.10252777570650957No Hit
AGACTTGGGTAAATACCGGATCAGAAAAGAATCATCAACGGATGCTAAATTT1100.10252777570650957No Hit
CGCGTAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1100.10252777570650957No Hit
ATTTGCGGGGTGGCTGTGGAATGATAAGGTCCTAGAGGGGCCCTGGCATTTC1100.10252777570650957No Hit
AACTTGGGGTACTCTCTATTTACTGACTACCAGAGATATACAAAATACTGTG1100.10252777570650957No Hit
GGCTTACAGATAAAAATGAAAGCATCAAGTGCCCAGTGAGTGAGACCCAGGT1090.10159570501826858No Hit
GCCAATGGGAAAGTGCTGGGACTATAGGAATGAGCCACCGCACCCAGCCTGG1090.10159570501826858No Hit
TGAAACGGACAAATATACAGCACAAAATTGTATTAATAAATTTTAAAAGAGA1090.10159570501826858No Hit
CAAGATGGGAGTGAAAACGGAACGTAAGTCATTTAGTCTGAGTTGGGAAATA1090.10159570501826858No Hit
ATAGCAGGGGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGC1090.10159570501826858No Hit
GAAGCAGGGGGATATTCAAAAGTTCTGTGGTGTTATGTCCAGTGTAGCTTTT1090.10159570501826858No Hit
CGAAGCGGGAGTCCAGCTGACGACTTGGGACAGACCTACAACCCGTGGCGGA1080.1006636343300276No Hit
GAATCCGGGGCTTGAAAGTATAAACCATCACTTAATTCTTATTTTAACTCTC1080.1006636343300276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)