FastQCFastQC Report
Sat 31 Dec 2022
SRR5846207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106137
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC2990.28171137303673555No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT2760.2600412674185251Illumina PCR Primer Index 8 (95% over 21bp)
GAGTAAGGGATGTAGAAAATTCCAAAGAATCAACAGAAAAACTCCTGGAACT1450.13661588324523966No Hit
ATGGCGATCTTTCTGCGGGTTTTGGAACCTGGTATGGTATTTCAGACGTCTT1400.13190499071954173No Hit
GCAGCGGGCCCCTAAGGAATAAGAATTATACATAAAGACGATTCTCAGCATC1400.13190499071954173No Hit
GAATCTGGGATAAAGACACCACTCAAAAGGAAACTTGAATAATTTATAATTT1390.13096281221440215No Hit
TATAAAGGGGGCCACAAAACACCAAAGAATTGTAGGCAGTGGCCCCTATTGA1380.13002063370926256No Hit
GAAATAGGGAATCACCACAGGACTATTCCTAGCCATGCACTACTCACCAGAC1350.1271940981938438No Hit
GGGCATGGGAAACCATACCCTGAAGTCCAGTCATTGACAAATCTTCCCGCTG1340.12625191968870422No Hit
CAAACTGGGGAACAGGCCTCTCTCTGCTGGCCGGCTTCGGAGGCTTCTTGGA1340.12625191968870422No Hit
TTAGTAGGGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACA1330.12530974118356464No Hit
GCGTAGGGAGCTGCGGGATGAAGAAGAAAAAATAGAAGAAAGAGAGAATGGG1320.12436756267842504No Hit
CACAGATGGTTTGGCCCTGCAGTGACCAGAGCAGCCCAAGCCGCCACCATGG1310.12342538417328547No Hit
TGGCTTGGGAATGCACAGTGAGGAAGACAGGTCAACTCAGTTGTGAACTACC1310.12342538417328547No Hit
GAGTAAGGGTGGCAAGGGACAATTATAGATTTCAGTATATTTTCTCAGAAAA1280.12059884865786671No Hit
GTAAGCGGGCTTATTCACTCTTTATTTGCTGCTGTTTAAGTTGCCAACCTCC1270.11965667015272714No Hit
TCTCCTGGGGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACT1240.11683013463730839No Hit
TCAAACGGGAGCGCTGGGGCCGTGGAGCCTTTGGTCACCTGCGCCTCTTCCA1230.11588795613216879No Hit
AATAAAGGGACTCCATGGGGAATAATTCTGATACTCTAATTAAGAGCAATAA1230.11588795613216879No Hit
GACTTGGGGAGATTCTGGAGGCGAAGAACGCAAAGCTGAGAACATGGACGTT1230.11588795613216879No Hit
GTCCAGGGGTCGAGCATTAAAGGGCAGTATTTAAAGTCAGTTGGCAAGCAGT1220.11494577762702922No Hit
CAATGCGGGAGGAGTTAGGACTTCAACATAAAAATTTTGTGGGGGACACAGT1220.11494577762702922No Hit
GTTATTGGGACAGATTTGGACATGTATTGCTTTCATTATCATTCAGTATTTT1220.11494577762702922No Hit
TGTCTAGGGATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGAT1220.11494577762702922No Hit
GAACAAGGGATGCCAGTTTCTAATGTTGGATTTGATGTAGACAGTTTGGAAT1210.11400359912188965No Hit
GGCACGGGTGAGTAAGAAGAGGGAAGTTACTGTCTATAAAGCTTTTTAAAAT1210.11400359912188965No Hit
AGTTATGGGCACATAATGAGCCGTTACAACTCCAGCACCAAAGCTAACGCCC1210.11400359912188965No Hit
TAATCGGGGTACCAAATCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTA1210.11400359912188965No Hit
GTTGCAGGGTCTTAAAAAAAGGCCTCCGATGAAGCAGAGGAAAGTGGATCAC1210.11400359912188965No Hit
GGCTGCGGGCGGGACGATGGCTTTGCCAGCTTAAAGTAACCGGATGGCGGAC1200.11306142061675005No Hit
GAATCCGGGGCTTGAAAGTATAAACCATCACTTAATTCTTATTTTAACTCTC1200.11306142061675005No Hit
TGCTGGGGGCCTGACCTCCCCTGACCCCGAAGATCACTACCTCTGTCATTCA1190.11211924211161046No Hit
CTGTACGGGCCTGGTTATCCAAGGATAGCAGAAATGTGTTTCTGTGGCTGAT1190.11211924211161046No Hit
ACTAGGGGGTGGCAGCCCGGCGCCATCTTCATCGAGCGCCATGGCCGCAGCC1190.11211924211161046No Hit
CAGGCGCGGGTGAGTACAAAAGTAATGAAATGGAGGTACATGCATCAAGCAA1180.1111770636064709No Hit
AAACTTGGGGTTTTAAGGGGACAGGCACATTGCAGTTGTAGGTGTTCATTTT1180.1111770636064709No Hit
TGTACGGGGTAATCACTCATTTTTAATCTACCACAATAAGTATGTGGCAAAC1180.1111770636064709No Hit
CACATCGGGCAGTGGGTCCATGCGCCTGACACTCCTCTCTGCTTGTGGACCT1180.1111770636064709No Hit
TGTAGCGGGTGAGAAAGAAATTTTACATTGCAACCCAGTGTGCGTGTACACA1180.1111770636064709No Hit
AATGATGGGATTGCAACAGTCCTCTGAATGTGACTGAATAAAGTTCATATTG1170.1102348851013313No Hit
ACACTCGGGACTTAACCAATATTATTGATTACCAGACTTTTATGAAAGCCAA1170.1102348851013313No Hit
ATTTGCGGGGTGGCTGTGGAATGATAAGGTCCTAGAGGGGCCCTGGCATTTC1170.1102348851013313No Hit
CACACAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1170.1102348851013313No Hit
ACTTGCGGGATATTTTGGAGGACGGGTGAAGAGGTAATAACGAAAGCAAGCG1170.1102348851013313No Hit
TGAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.10929270659619172No Hit
TCATATGGAGGAGGAGATTGGGGACCTGACGTTCACTGTGGCCCAAAAGATG1160.10929270659619172No Hit
AAGTCGGGGGTGATCCTTTAGAACCAACTCAGACACCAGATCCACTGGAAGA1150.10835052809105213No Hit
TACAAAGGGCATGGACTTTAAAATAATTGTTACTTTACACGTGTTTGAAATC1150.10835052809105213No Hit
AGACTTGGGTAAATACCGGATCAGAAAAGAATCATCAACGGATGCTAAATTT1150.10835052809105213No Hit
CGATGGGGGAACATTATGAAGGTGGTTTTGAAGATAATGCTAGAATTTATGA1140.10740834958591254No Hit
ATTAGTGGGATAGCAAAACATTTTACTTTCATTTTTCAGTATTTGCTGCTTT1140.10740834958591254No Hit
ATGAATGGGATTACCAAACAAGAGTATTTTTATGAAAGAAAAGGACAAACCT1140.10740834958591254No Hit
ACTATAGGGACCATGAGGCAGGCGACCTTGGCTATCAGACTTGAAATATTCA1140.10740834958591254No Hit
CAACTAGGGATTGTAAAGGAATTTGAAGATAGAGGATTTGTGCAGGTGGATG1140.10740834958591254No Hit
TTGTAGGGCACTTGCCAAACTTAACTTATTTAAACTTAATACCAAACCCATA1130.10646617108077297No Hit
TTATCAGGGGAAAAAAAAGGAAAGAAAAAATCTGTAGGTCGTCCACCTGGCC1130.10646617108077297No Hit
ACGCCAGGGGAGGCAACTCCCAGGCCAGTGAGCTTTACTTGCCGTGTAAAAG1120.10552399257563337No Hit
CTCACTGGGAAATACTATAATCACAGAGCTTGCAGAAGCTGCCTGGAGGACC1120.10552399257563337No Hit
TCTCTCTGGGAGGCAGACTTTGTCTTCTCTTTAGTATACATTTCTGGAAACC1110.1045818140704938No Hit
GATGCTGGCGAAGGAGTCTCATGCTTTCTTCACAAGAGAGTTCACAGGATTA1110.1045818140704938No Hit
ATTTGTGGGCTGTTTAGTGTTGTATTTCAATTGTGGAGCCTCATTTTAAGTG1110.1045818140704938No Hit
GCATGTGCTTGAAAATGACATTTTATTTCCCGGATGCTGTACCTCTGCTGCC1110.1045818140704938No Hit
AATGAGGGGGGCGGAGGCCGAGCACTCGGCGCTGAACCCGCTGCGCGCGGTG1110.1045818140704938No Hit
TATGGTTGGGCAGAAATAAGTGAAAACATGTTACTTGACAGTCAAGTGTGGT1110.1045818140704938No Hit
CATCTTGGGAATGCTGTTGTAGTCGTTTCCTCAGCCTCATCTCACTGTGCTG1110.1045818140704938No Hit
ATGTTGGGGATGCCCAGATAGCCAGGGTGCCTAACAAGTAAACAGAAGCTGA1110.1045818140704938No Hit
GGGCCAGGGGAAAGAAGCATGAAGAAAACAGCTGCATTTGAAGAGAAACGGA1100.10363963556535422No Hit
TTGCGGGGCTGAGCTTGATTCTAAAGGGGACAGATCTTGAGCAAGGCAAGAA1100.10363963556535422No Hit
AACCGGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAG1100.10363963556535422No Hit
TCACTGGGGTTTGGGCCTCCCTCTTGGTTGAGCTGAGTGTGCCTGAGTGGGT1100.10363963556535422No Hit
TATAGTGGGTGGAAGGGCAAACAGAAAAACCCAAGACCTACAAAGGCTGACT1100.10363963556535422No Hit
CTAGCCGGGATTTTTGGTCTTTGTTTATTCATTTAGACCCTGCAAGTTGATT1100.10363963556535422No Hit
GTATATGGGAACAAAGGAATTAAAATGGGATACTAAACAAAAATATTTGACA1100.10363963556535422No Hit
TGAATAGGGATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACA1100.10363963556535422No Hit
ATTTAGGGGTAACATGTTTATTGAAAACAAGAAAGCACAGAAAATATCAACT1100.10363963556535422No Hit
AAAGCGGGGATTTCTACCCAGGGACAAATGAGGAATATAACTAGCAGAGATT1100.10363963556535422No Hit
TGTCAATACAAAGATTGCCCTAATTACAGTTTCAGATAAGGACACAGATGTG1090.10269745706021462No Hit
AGGTTTGGGTACATACATAAATAGAAAACCTGGCAACTTACTAATGTAACTT1090.10269745706021462No Hit
TTTGCCGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1090.10269745706021462No Hit
TCGTACGGGGAAACAACAACAAAAAAACAAAAGCAGTAATAACTGCTAAATA1090.10269745706021462No Hit
TAAAAAGGGGCAAGAATGGCTTTGATTGGACTTGGTGTGAGTCATCCTGTTC1090.10269745706021462No Hit
TCAGTGGGGCGATCTTGCGGTGGGATAAAGAAGATAGAAAAAAAAGACTTAG1080.10175527855507505No Hit
GAATAAGGGAGGTCAACATAAGGTCCCAGGGCATAAGAAAGAAGACTGGTTA1080.10175527855507505No Hit
ACATAGGGGATTCTGCTATCTACCAAAGATATGCTGATTTTAACTGATTTAC1080.10175527855507505No Hit
AAGCAAGGGCACTGAAGAGACGTGAAGTGTACATTCAACTGAAGTGTCATTG1080.10175527855507505No Hit
TGAAACGGACAAATATACAGCACAAAATTGTATTAATAAATTTTAAAAGAGA1080.10175527855507505No Hit
GCGTAGGGATAAAGCTTTAGGAAGGCAAATGTGTTTTTTTCCCCTTGGAATA1080.10175527855507505No Hit
AGCTTAGGGGGGGGCGGCGCGGGGCGCGGACACTCCCCGCTGAGAGTCCGCC1080.10175527855507505No Hit
CGCGTAGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT1070.10081310004993546No Hit
TAACATGGGTGTTTGTTTCATCTTTGTCTTCTCTGCTCTGGTGGAGTATGGC1070.10081310004993546No Hit
GTAAATGGGGAACTTACCTGATATTTTTCAAAACACATTTTTAGAATTGTTT1070.10081310004993546No Hit
TTACAGGGGTTAACAGTTCATTAAAACTGACTTTTCTTTGGCATAACAAAGG1070.10081310004993546No Hit
ACATTTGGGCTTATTTCAAATGTATGAAATATCTTCTAAAACCCTATTTCTT1070.10081310004993546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)