FastQCFastQC Report
Sat 31 Dec 2022
SRR5846170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97466
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT5540.5684033406521248Illumina PCR Primer Index 8 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC4910.5037654156321179No Hit
TTACGAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1580.16210781195493812No Hit
CAAGCTGGGACTGCTTGATAATCACTCTCAGGTGTAAAGCTCCAAGTGTAAA1520.15595181909588984No Hit
GATCCTGGGGGCTCAAGCCTGACGCCTCTGAAAAGGCAGCAGCAGTCGCCTT1500.1538998214762071No Hit
ACAAGAGGGATGGATTAATTGCCTCAAATTTGTATAAGTGATTGGCTAGTGA1430.14671782980731743No Hit
TCACTTGGGAGGCAAAGCAGTCTGAGAAAGAAATAGGAAATGCAGAAATAGG1390.1426138345679519No Hit
TTGCACGGGATTGTCTTTCTGGTGTCCTGGGCTGTTTTGAAAAAGTTTCCAT1370.14056183694826913No Hit
GGCTGAGCCGCTTGCCTGACTTTTGATCCGACCAGTAATCACTTTGCCCGTG1360.13953583813842776No Hit
TAGTACGGAGACAAATTAAATTTGGCAGAGTTTAATTGAGCAAAGGACAATT1360.13953583813842776No Hit
ACCATAGGGCATCTTTGTTAGAAATAATATGCAAATTTTTCCAAGAGGTTAT1320.13543184289906224No Hit
AAAACCGGGGATCAAAACGATGCACTGCAAGGTTAACATCCAATTTTTAATA1320.13543184289906224No Hit
TGCAATGGGGGTATTGGAGGGTGAGTAAAACCTTGAATGTGAAAACTTAAGC1310.13440584408922085No Hit
CATACGGGAGGATAGAGACTCATTCTCATCTATTTTGGTTAAAAGAAAATAA1280.1313278476596967No Hit
TTCATAGGGGATGACGCGAAAGTAACCGAGACTATCAGGATCCGGAGACGGA1280.1313278476596967No Hit
CGCGTGGGGCCTGGGGGCTGTGCCCCCATCTCCTCCCCACCCCAGGTCTAGT1280.1313278476596967No Hit
CAATAAGGGCTAGTTCAAAAGGTAAGCTGCTTTCGTCAAAAGTTTTGAAGTA1260.12927585004001396No Hit
GTTAAAGGGCTGTGCAAACATATCATCTCTTCAAATGCTGCACACTTTGCTT1250.12824985123017257No Hit
AGTAGTGGGACCAATAGGAATGGATCGATAGTTGAATAATGATTTTTTATAC1230.1261978536104898No Hit
CACTGGGGGGTGCGAAGCCTGCCGCTGCTGCAGCGAGTCTGGCGCAGAGTGG1220.12517185480064844No Hit
TATCTTGGGGTTTTGTTGTTATTAGTAATTTCTAACTGCCCTAAATTTGTCT1210.12414585599080705No Hit
TACAGGGGGCACCCGTGTGACTTTCGTAACGGGGAGAGAGAGAAAAGATCTC1210.12414585599080705No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1190.1220938583711243No Hit
TGATCAGGGAGTTTGGGCATTAAACAAGTAGTTTCTTAAATGCTGAATTTCA1180.1210678595612829No Hit
GCCACCGGACAGATTTTCGTGATATATTTATACACGCATGGGTTTGTAGATC1170.12004186075144152No Hit
ATAAGAGGGAGGAGGAGAGAAAAGACCTTTCCAGAATCTTTAACATAAGATT1170.12004186075144152No Hit
TGGCTTGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA1160.11901586194160016No Hit
CCACCAGGATGGTACATTTATTAAAATGAATTGACCTGCATTGACACATCTT1150.11798986313175876No Hit
TAAACTGGGTTACAGTGCACTGTTCATTTAGAAGACAATTCAAGCATCACCT1150.11798986313175876No Hit
GCGGCTGGGAAGAGGCCATTGGAAAAAGGCCATTTACAAGTTAATGTTACTT1150.11798986313175876No Hit
TAAACTGGGAAATCAAGGTGAATGAGGTAGCATCTCTCTGTAAAGCAGAGAG1150.11798986313175876No Hit
TAGCATGGGCAACATACCAGAATCTCTGGGACACATTCAAAGCAGTGTGTAG1150.11798986313175876No Hit
GTTAGAGGGAACCTTTTGTAAGTTGAAAATATCTTAAGTCAAAAATGCATTT1150.11798986313175876No Hit
TAAGCCGGGGTTATTGAGAAGCTGTCTTATTTCAAAATTGGTTAGTCAGTTG1140.11696386432191738No Hit
TGCCCAGGGAGCCATATCCCAGCCCCGTCTGCAGGGAGCCGGAGGCTGCTGC1130.11593786551207601No Hit
ACAGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1130.11593786551207601No Hit
TGCATGGGGATAAAGGGAAAGAAAAAAGAGTTTTCTTACACAATTTTAACAT1130.11593786551207601No Hit
TTGCGCGGGAACAACAGGGCCAGATCCTAAAGCTCTTTCAGGGAGCTCTTCT1110.11388586789239324No Hit
AGATGGGTATTTCCCTAAGATCTACTAGTGATAATTCTACCTTAACTGTAAG1110.11388586789239324No Hit
GGGCATGGGCAGTGTGTGGTTTATAGACTCATGAGGGATCCCAATACCAGTT1110.11388586789239324No Hit
ACACCTGAGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGAG1110.11388586789239324No Hit
CTACATGGGCAGTTTAAACAAAAACAAAAACCAGACAACAACCTTGCAAGGG1100.11285986908255187No Hit
CTAGAAGGGGGTCGGGGGCATGCTGCTCCAACAGTTTCCCTTGTTAGCAGTC1100.11285986908255187No Hit
TGATCTGGGCTGGATATCCATATGCAGAAGAATGAAACTAGACCCCCATCTT1100.11285986908255187No Hit
GCCTTGGCCTCCCGAAGTGCTAGGATTACAGGCTGAGCCACCACGCCCGGCC1100.11285986908255187No Hit
TTGAACGGGCCGTGTCAACGAGGCTGCTTTACAACAGCTTTATTTATTTTTA1100.11285986908255187No Hit
TACCATGGGTGATGTACGTAGATTATATTACAGTGAATTATTTTGCGTTTTT1090.11183387027271048No Hit
TTTAGAGGGGCGATCTGTACTCGGGGAGAGCAGGACTAAAGACATACTTAAA1090.11183387027271048No Hit
TCACAAGGGGTTTCCAAGTTGGGAATTACCTCCTACTTCTTGAAAGTTACCC1090.11183387027271048No Hit
GTAAGGGAAAGTGCCTGTGTTCATTGTGGACAAAGTTATTATTTTGCAACAT1080.1108078714628691No Hit
AACGCTGGGATGAGTTGTCTGTCGACCCCTGCTGGGAGGTGTCTCCCATCAG1080.1108078714628691No Hit
CTATGAGGGGCAGACGTCGGGGAGGTGAAACACGTCTCTTCTCTACCAGCTG1080.1108078714628691No Hit
AGCTGAGGCTCCAAGAAGTCACACAACTAAAATGTTAGAATTTAACTAAAAT1080.1108078714628691No Hit
ATTTCCGGGTGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAATTTTACTTG1080.1108078714628691No Hit
GTCAGAGGGACCTGAAATTGTGTAGATGGGGTATGGTGGTCTCCATCCACCA1080.1108078714628691No Hit
AGATCAGGGGGCCGCGATGGTGTTGTTGGAGAGCGAGCAGTTCCTGACGGAG1080.1108078714628691No Hit
TTAGCCGGGTCAAAAGCACCTGGAACGAGTAATTGAAATGATTCAGAATTGC1080.1108078714628691No Hit
ATCTTGGGGCTATGACAGGTTTCTTAGAAATCTGTGTTTCTACTATGAAACA1070.10978187265302773No Hit
TAGTTTGGGAGAAAATGGAATTGGAGGAACTAGTAGAATTCAGGGTAATATC1070.10978187265302773No Hit
TGCTATGGGGTGCCTCTTCGGGTTGTGTAGAAGTAGGAAATGTGTCACCAAC1070.10978187265302773No Hit
TACAATGGGACTTGTGTTTCTATACACTAATAGCAATAAACCAACCAAAAAG1070.10978187265302773No Hit
ACGACAGGGATTCCTATAGCTGCCTCTGGAAGCATCCAGATGGCATTTTAAA1070.10978187265302773No Hit
TTAAAAGGGAGCAGATATTTATAGAACATTCTATCCAAAAGCTACAGAATTT1070.10978187265302773No Hit
TTTCAAGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1070.10978187265302773No Hit
TGATATGGGTAAAAGCTTACGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAA1070.10978187265302773No Hit
GAGTATGGGCATTCAGCACATTTTTATTGTTTTTGCAAGATGATGTTTCACT1070.10978187265302773No Hit
AAAAGTGGGGAACAATAGGAGTTCCTGTGCTTGCTTAGTCATGAAAGCCAAT1070.10978187265302773No Hit
GTCTGCGGGATTTTGTGATCATTACAAATTCTTACACTGAATATTTTTATTT1060.10875587384318634No Hit
CTTGCCGGGCGATCTAGGCGTGGGTGACGACGAGGAGGAAGACCTGCTGGAT1060.10875587384318634No Hit
CTATAGGGGGGCAGTGTTAAGTGGGAAATTCATAGCACCAGATACCCATATC1060.10875587384318634No Hit
GTTCTGGGGATTCGTGTGTGTCTGCTTTTAAACTTGGCTGGGCTATCAGACC1060.10875587384318634No Hit
AAGGCTGGGATTGCTTATGGCAGTGTCAGCCTAAGAGCTCAAGTTCAAGATC1050.10772987503334497No Hit
AGACTAGGGACGTGAAAATATATGTACTCTTCACTTTTGTTCCAGTACTATA1050.10772987503334497No Hit
AAGCAGGGATGCATGGGTTGGCCTAACCCACAAACCATTTCATACTGAACAT1050.10772987503334497No Hit
TCCAATGGGATGTTATATCAAGGGAGGGGGCATAAAAGAAATACAAAAATTG1050.10772987503334497No Hit
ATTTCAGGGGGAAGTGGGAGGCCTGTGTTCTTCACTGCACCAGTCTGGAGTG1050.10772987503334497No Hit
GCGCACGGGCATGTTTTTAGGTGACTTTTTTCCCCCTCTTAGTACTCTGGAG1050.10772987503334497No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.1067038762235036No Hit
ATTGCCGGGGAAAATTGCCACTGTTCCCTAAGAAAATGTTATTGATGGTAGG1040.1067038762235036No Hit
ATTACAGGGGAGCGCGGCGGAGCAAGCGCAGAAGGCTGGGAGGGCTGCGCGG1040.1067038762235036No Hit
ATCAACGGGAGACTTCTCACTTCTGGTTGGAGGTTTCACATATGGCTCAACT1030.10567787741366219No Hit
AAGTGAGGGAAAAGGATCTTCTGAGAATCACTAGACAAGATTGGAACCATGG1030.10567787741366219No Hit
TTATGTGGGGAAACCAACAACAAACATTTTTAAAATTAAAATGGATAATTTG1030.10567787741366219No Hit
GTAGGTGGGTAACTTTGTATACTTCCTGGTTTTTGAAACGTGTGACTGTGTT1030.10567787741366219No Hit
AGCAATGGGGACGCTCTTCCGATCTTCTTGAGGGAAGACACAGACTGGCAAA1030.10567787741366219No Hit
CTCAGTGGGTATGCACCAATAACATAATCTGGCTACAAGATAACCGTTATTC1030.10567787741366219No Hit
TAGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1030.10567787741366219No Hit
CAAAGCGGGTATGGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGT1030.10567787741366219No Hit
AATGCAGGGAACAGCCGCCCGAAGGAAGCACCATGATTTCGGCCGCGCAGTT1020.10465187860382083No Hit
TGCCAAGGGGTACGCTTGCCTACCGGGAAGAGTGAAAAGATGTTAAAGAAAA1020.10465187860382083No Hit
GTACAGGGGTACTTGCCCTTGGCAGGTGTGGGATCCAGGCCAGTTGCATGAG1020.10465187860382083No Hit
CTCGCAGGGCACATAACCACAGATATACTCAACTGTTTCAGAATTTACTAAA1010.10362587979397943No Hit
AATTAGGGGGCCAGTGAAAACATTAGATTGGGCTGGAACATGGAAAAATTAA1010.10362587979397943No Hit
AACAGTGGGAAGAGATTGACTCGGCATTGCCTTCATTCCTTAAAATTAAAAA1010.10362587979397943No Hit
ATTCACGGGGATTCTTGTAGTGCATGATGACTTTGAAAATGTTTGGCCATTT1010.10362587979397943No Hit
ATAAATGGGATATGGAAACTGGTTAGGTACAATACTTACAGTGCCTGTATTG1000.10259988098413805No Hit
TAGCTTCGGGGTGTTTTAAAATTACACTTTTTCTTAACCTGTCTAGCTGTCG1000.10259988098413805No Hit
TTTATGGGGTCATGGGCCAAATTCAGCTAGGTGCTTTTTTGCGTAGCCCTTG990.10157388217429668No Hit
GCCACGGGATATTTACTCTACTGGAAATTAAAAGTGAGAAATTTATAACATG990.10157388217429668No Hit
TGAAAAGGGCGATCTTTTGCCGGGTGTAATTAGTATGAGAAATTATTTCATG990.10157388217429668No Hit
TACGCTCGGGACTATAACTAAGTCAACTCAAATTTATCATTTCTATAATTAT990.10157388217429668No Hit
GCACATCGGAGGAAAACTCGGTAAAGCAAAATGATGTTGATATGTTGAATGT990.10157388217429668No Hit
TAGCAGGGATGAGACAGAAGGTTAACAAGGATATCCAGGACTTTGGCTCAGC990.10157388217429668No Hit
TTGCTCGGGCATTGTGTCACTGAAATGTTTAGCTTACTTTAATCTTGATATC990.10157388217429668No Hit
AAACTAGGGAAGAACAATTTTTAAAAAAATTGTTGTGGAATGATAGTTTCTA990.10157388217429668No Hit
GGTTGGGGCAATATTGAATAAGACCTTTAGATCATTTGTGTGTGTGTGTGTG980.10054788336445529No Hit
CGCTTGGGGGGCGCAGGCGCGGAATCTCCGACTTGCCATGGTCGTCTTTGTC980.10054788336445529No Hit
GACAAGGGGTGGCCCAGGGAAAATAATTTTGCTGCTACTAATTCTAGGTTGC980.10054788336445529No Hit
TTCGTGGGGGCCCATCTAGAAACTGGTGCTTTAAACTGTTTTTTTGGATGGG980.10054788336445529No Hit
AGTAAAGGGCAGCATCACTAAACCGATAATGCATGAAGCAACGATAAAATTA980.10054788336445529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)