FastQCFastQC Report
Sat 31 Dec 2022
SRR5846169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98129
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC5340.5441816384555025No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT5300.5401053715007795Illumina PCR Primer Index 8 (95% over 21bp)
GGCTGAGCCGCTTGCCTGACTTTTGATCCGACCAGTAATCACTTTGCCCGTG1530.1559172110181496No Hit
CTCAGTGGGTATGCACCAATAACATAATCTGGCTACAAGATAACCGTTATTC1530.1559172110181496No Hit
TAGTACGGAGACAAATTAAATTTGGCAGAGTTTAATTGAGCAAAGGACAATT1520.15489814427946885No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.14776467710870383No Hit
TCACTTGGGAGGCAAAGCAGTCTGAGAAAGAAATAGGAAATGCAGAAATAGG1420.1447074768926617No Hit
GTAGGTGGGTAACTTTGTATACTTCCTGGTTTTTGAAACGTGTGACTGTGTT1410.14368841015398098No Hit
CAATAAGGGCTAGTTCAAAAGGTAAGCTGCTTTCGTCAAAAGTTTTGAAGTA1400.14266934341530027No Hit
TATCTTGGGGTTTTGTTGTTATTAGTAATTTCTAACTGCCCTAAATTTGTCT1400.14266934341530027No Hit
TTGAACGGGCCGTGTCAACGAGGCTGCTTTACAACAGCTTTATTTATTTTTA1400.14266934341530027No Hit
TTGCACGGGATTGTCTTTCTGGTGTCCTGGGCTGTTTTGAAAAAGTTTCCAT1390.14165027667661956No Hit
GCCACGGGATATTTACTCTACTGGAAATTAAAAGTGAGAAATTTATAACATG1380.14063120993793884No Hit
TTACGAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1380.14063120993793884No Hit
AACGCTGGGATGAGTTGTCTGTCGACCCCTGCTGGGAGGTGTCTCCCATCAG1320.13451680950585454No Hit
AAAACCGGGGATCAAAACGATGCACTGCAAGGTTAACATCCAATTTTTAATA1310.13349774276717383No Hit
CGCGTGGGGCCTGGGGGCTGTGCCCCCATCTCCTCCCCACCCCAGGTCTAGT1310.13349774276717383No Hit
CTATAGGGGGGCAGTGTTAAGTGGGAAATTCATAGCACCAGATACCCATATC1300.13247867602849311No Hit
GTCAGAGGGACCTGAAATTGTGTAGATGGGGTATGGTGGTCTCCATCCACCA1300.13247867602849311No Hit
AGCAATGGGGACGCTCTTCCGATCTTCTTGAGGGAAGACACAGACTGGCAAA1270.12942147581245095No Hit
TATAAAAGGGATGGAATTTCATGTGCCATAAATAAAATTGTCTTGATTTGTC1260.12840240907377024No Hit
ATTTCAGGGGGAAGTGGGAGGCCTGTGTTCTTCACTGCACCAGTCTGGAGTG1260.12840240907377024No Hit
TAGCATGGGCAACATACCAGAATCTCTGGGACACATTCAAAGCAGTGTGTAG1260.12840240907377024No Hit
TGCCAAGGGGTACGCTTGCCTACCGGGAAGAGTGAAAAGATGTTAAAGAAAA1250.12738334233508952No Hit
CACTGGGGGGTGCGAAGCCTGCCGCTGCTGCAGCGAGTCTGGCGCAGAGTGG1240.1263642755964088No Hit
ACCATAGGGCATCTTTGTTAGAAATAATATGCAAATTTTTCCAAGAGGTTAT1240.1263642755964088No Hit
CTAGAAGGGGGTCGGGGGCATGCTGCTCCAACAGTTTCCCTTGTTAGCAGTC1230.1253452088577281No Hit
GTTAAAGGGCTGTGCAAACATATCATCTCTTCAAATGCTGCACACTTTGCTT1220.12432614211904738No Hit
CATACAGGGAAAGTGGGGAAAGGATACCCTTTTTAACAATAGCACTGGGATA1220.12432614211904738No Hit
GCCACCGGACAGATTTTCGTGATATATTTATACACGCATGGGTTTGTAGATC1210.12330707538036666No Hit
GTAAGGGAAAGTGCCTGTGTTCATTGTGGACAAAGTTATTATTTTGCAACAT1200.12228800864168596No Hit
TACCATGGGTGATGTACGTAGATTATATTACAGTGAATTATTTTGCGTTTTT1200.12228800864168596No Hit
CATACGGGAGGATAGAGACTCATTCTCATCTATTTTGGTTAAAAGAAAATAA1200.12228800864168596No Hit
AACTGCGGGGCATCCTGAGACCTTGTGTCCCTGAGTCTCCTTTCTGAGTGCA1200.12228800864168596No Hit
AGTAGTGGGACCAATAGGAATGGATCGATAGTTGAATAATGATTTTTTATAC1190.12126894190300523No Hit
CGTAGTGGGCTCAGGAGTTTCTGAGAACGACTCTCGCCCAGCTTCTCCAAGT1190.12126894190300523No Hit
GTACAGGGGTACTTGCCCTTGGCAGGTGTGGGATCCAGGCCAGTTGCATGAG1190.12126894190300523No Hit
TATTCTGGGACCCTGGCAGAAGGGAGAGAAATGTGTTTTGGGGTGGGTAACT1170.11923080842564379No Hit
TTGCGCGGGAACAACAGGGCCAGATCCTAAAGCTCTTTCAGGGAGCTCTTCT1160.11821174168696308No Hit
GTTAAAGGGGTGGGGATCCAGGATTTGTAATTTCTTGATCTAAACTTTATGC1160.11821174168696308No Hit
CAAGCTGGGACTGCTTGATAATCACTCTCAGGTGTAAAGCTCCAAGTGTAAA1150.11719267494828237No Hit
ATACAAGGGGGGAGGCATGGGGGGTGATTAAATTATCATTTCCAAGGTGCAA1140.11617360820960164No Hit
TGCAATGGGGGTATTGGAGGGTGAGTAAAACCTTGAATGTGAAAACTTAAGC1130.11515454147092094No Hit
AAGGCTGGGGTGCATGAAAAGTGACAAGCATGGGTCCCCATATTTCTACTTA1130.11515454147092094No Hit
TTGCTCGGGCATTGTGTCACTGAAATGTTTAGCTTACTTTAATCTTGATATC1130.11515454147092094No Hit
AAACTAGGGAAGAACAATTTTTAAAAAAATTGTTGTGGAATGATAGTTTCTA1130.11515454147092094No Hit
ATTTCCGGGTGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAATTTTACTTG1130.11515454147092094No Hit
CTACATGGGCAGTTTAAACAAAAACAAAAACCAGACAACAACCTTGCAAGGG1120.11413547473224021No Hit
CGTCTAGGGGGCTGTGGGTTTATCATAAACAGCTCTTATTATTTTGAGATAC1120.11413547473224021No Hit
TAAACTGGGTTACAGTGCACTGTTCATTTAGAAGACAATTCAAGCATCACCT1120.11413547473224021No Hit
TAAGCCGGGGTTATTGAGAAGCTGTCTTATTTCAAAATTGGTTAGTCAGTTG1120.11413547473224021No Hit
TTAAAAGGGAGCAGATATTTATAGAACATTCTATCCAAAAGCTACAGAATTT1110.11311640799355951No Hit
TACAGATAGGAAACTATGCCCTGATTTCTGAGCCTTGACATCATCACATTCT1110.11311640799355951No Hit
GACACAGGGATGGGTGAAGAACGAACTATCAGAATGAGTCTGTGAAGGGTAA1110.11311640799355951No Hit
TTCATAGGGGATGACGCGAAAGTAACCGAGACTATCAGGATCCGGAGACGGA1110.11311640799355951No Hit
GGTTGGGGCAATATTGAATAAGACCTTTAGATCATTTGTGTGTGTGTGTGTG1100.11209734125487879No Hit
AGATGGGTATTTCCCTAAGATCTACTAGTGATAATTCTACCTTAACTGTAAG1100.11209734125487879No Hit
CGCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.11209734125487879No Hit
TTTAGAGGGGCGATCTGTACTCGGGGAGAGCAGGACTAAAGACATACTTAAA1100.11209734125487879No Hit
CTCAATGGGATCATAATCTCTTTGAAGAAATTAAAACTTATTTTTGTCAGTT1090.11107827451619806No Hit
GCCAAGGGGCCTACTGGGAAGTGAGGAGCCCCTCTGCCCGGCCACCACCCCG1090.11107827451619806No Hit
TTTCAAGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTG1090.11107827451619806No Hit
TCTTCAGGGGCCAAAAGAAACTTTCTAAACAATGTATAGGTACACAGCAAAA1090.11107827451619806No Hit
TGCCCAGGGAGCCATATCCCAGCCCCGTCTGCAGGGAGCCGGAGGCTGCTGC1080.11005920777751735No Hit
ATTACAGGGGAGCGCGGCGGAGCAAGCGCAGAAGGCTGGGAGGGCTGCGCGG1080.11005920777751735No Hit
GTTAGAGGGAACCTTTTGTAAGTTGAAAATATCTTAAGTCAAAAATGCATTT1080.11005920777751735No Hit
TGCTATGGGGTGCCTCTTCGGGTTGTGTAGAAGTAGGAAATGTGTCACCAAC1070.10904014103883662No Hit
CTTCATGGGCTGGTTCTTACAGGAACCACCTACTCTTTCAGTTTTCTGGCCC1070.10904014103883662No Hit
TCCAATGGGATGTTATATCAAGGGAGGGGGCATAAAAGAAATACAAAAATTG1070.10904014103883662No Hit
GAGTATGGGCATTCAGCACATTTTTATTGTTTTTGCAAGATGATGTTTCACT1070.10904014103883662No Hit
CCATGCTGGGGTATGTTTTCTTTAAACCTGTTTTAAGAAAGCAGCTACCTGT1060.10802107430015592No Hit
AACTCTGGGCTCAGCAGCTGATTCCAGGAGAGTAATTAGGAAAAGAGCCAAT1060.10802107430015592No Hit
TCAGCAGGGAACACTGCAACTTTATCATAGCAGTTTTCATCCCAGAATTTTT1060.10802107430015592No Hit
GTAACTGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT1060.10802107430015592No Hit
CTTACCGGGGGGCGAGCCGTTCTTCACTACCAGCCTGCTGCCGTGGCACAAC1060.10802107430015592No Hit
ATCTTGGGGCTATGACAGGTTTCTTAGAAATCTGTGTTTCTACTATGAAACA1050.1070020075614752No Hit
ACTCATGGGTAGACCCTCACACAATAATAATGGGAGACTTTAACACCCACTG1050.1070020075614752No Hit
GCCTTGGCCTCCCGAAGTGCTAGGATTACAGGCTGAGCCACCACGCCCGGCC1050.1070020075614752No Hit
ATAACAGGGCTTATAAACATTCTTTAACTTTTTTGTTTGTTTGTTCTCTTTT1050.1070020075614752No Hit
GGCTACGGATGTGAAGTCGTATAATAACACTTGAAGGTAGACTGATTAAAGA1050.1070020075614752No Hit
ACAAGAGGGATGGATTAATTGCCTCAAATTTGTATAAGTGATTGGCTAGTGA1040.1059829408227945No Hit
AACGACGGGCCTAGACCTGCTGAAATAGACTTTATTTTGGCTAGATGTTCAG1040.1059829408227945No Hit
TTATGTGGGGAAACCAACAACAAACATTTTTAAAATTAAAATGGATAATTTG1040.1059829408227945No Hit
CAATCTGGGCTCCCACCTCAGCCTCCAGAGTAGCTGAAACCACAGGTGCGCA1040.1059829408227945No Hit
TTACCTGGGATATTTATGGTAAATCTATTTTCAATTTGTGCACAGGCATTTT1040.1059829408227945No Hit
TGGCTTGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA1030.10496387408411377No Hit
GATCCTGGGGGCTCAAGCCTGACGCCTCTGAAAAGGCAGCAGCAGTCGCCTT1030.10496387408411377No Hit
TACAATGGGACTTGTGTTTCTATACACTAATAGCAATAAACCAACCAAAAAG1030.10496387408411377No Hit
AATTCAGGGATCTAAGCTTGTGATCATACTAAAACATTTTCACCTCTTAAAA1030.10496387408411377No Hit
TGATATGGGTAAAAGCTTACGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAA1030.10496387408411377No Hit
TAGCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1030.10496387408411377No Hit
TAAGCAGGGAGAAGCATCAAGCCCAGGGGTAAAGGCTGAAGCTTTCACAGTG1020.10394480734543306No Hit
TGATCAGGGAGTTTGGGCATTAAACAAGTAGTTTCTTAAATGCTGAATTTCA1020.10394480734543306No Hit
TACAGGGGGCACCCGTGTGACTTTCGTAACGGGGAGAGAGAGAAAAGATCTC1020.10394480734543306No Hit
TATAATGGGGAAACTACTGCTGTTAGAGCACTTCTTATTCATTGTCTTTTAG1020.10394480734543306No Hit
GTTCTGGGGATTCGTGTGTGTCTGCTTTTAAACTTGGCTGGGCTATCAGACC1020.10394480734543306No Hit
GTATAGGGATATAAAGAATTTAGTCCTAAATTGATTTTAAGAAATTGCGTAT1010.10292574060675233No Hit
CCACCAGGATGGTACATTTATTAAAATGAATTGACCTGCATTGACACATCTT1010.10292574060675233No Hit
AACAGTGGGAAGAGATTGACTCGGCATTGCCTTCATTCCTTAAAATTAAAAA1010.10292574060675233No Hit
AAGCAGGGATGCATGGGTTGGCCTAACCCACAAACCATTTCATACTGAACAT1010.10292574060675233No Hit
AGCTGAGGCTCCAAGAAGTCACACAACTAAAATGTTAGAATTTAACTAAAAT1010.10292574060675233No Hit
TTTGAAGGGTTGATACTATTTTCTAGATCCTGTTGGCATGCTTTACGGTTTT1010.10292574060675233No Hit
CATTCCGGGATGTCCATGTCAAAATTCACTTTAGTCAGAACCAGAGTATTGA1010.10292574060675233No Hit
AATGCAGGGAACAGCCGCCCGAAGGAAGCACCATGATTTCGGCCGCGCAGTT1000.1019066738680716No Hit
CACTGTGGGCCACCTATTTAAAAATGAACTCCATGTCCGTCGTTTCCCACCA1000.1019066738680716No Hit
GCGATAGGGGTAAAAGGGTACTTCAAAATCCATGCCATCTTTTTTTAGTGGG1000.1019066738680716No Hit
ACTAACGGGACCTTAAAGGAACTCAGCTCATTTGAATGTATTGAGTTTTGGA1000.1019066738680716No Hit
TAAGTCGGGCGATCTTGTGCCGGGTCCGACAGCAGCTCCCTGGGCAGTGGCC1000.1019066738680716No Hit
TCACAAGGGGTTTCCAAGTTGGGAATTACCTCCTACTTCTTGAAAGTTACCC1000.1019066738680716No Hit
TCCTAGGGGTGAGCACACATGTAACTTTCACACTCCTTGCAATTTCTAGGAG990.1008876071293909No Hit
GGTATAGTGGGCAAGTGAAATTGCTATAATTTTAGTTCTTTGGTTTGAAAAC990.1008876071293909No Hit
TAAACTGGGAAATCAAGGTGAATGAGGTAGCATCTCTCTGTAAAGCAGAGAG990.1008876071293909No Hit
TGATCTGGGCTGGATATCCATATGCAGAAGAATGAAACTAGACCCCCATCTT990.1008876071293909No Hit
TGTCTAGGGGGCTCTAAGATGCGGAAGAGTCACTGCATCTTCTTGAATGTTA990.1008876071293909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)