FastQCFastQC Report
Sat 31 Dec 2022
SRR5846168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96894
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGCCGTCT5110.5273804363531281Illumina PCR Primer Index 8 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCTCGTATGC4720.48713026606394616No Hit
CAATAAGGGCTAGTTCAAAAGGTAAGCTGCTTTCGTCAAAAGTTTTGAAGTA1510.15584040291452517No Hit
ACCATAGGGCATCTTTGTTAGAAATAATATGCAAATTTTTCCAAGAGGTTAT1380.1424236794847978No Hit
CTCAGTGGGTATGCACCAATAACATAATCTGGCTACAAGATAACCGTTATTC1370.14139162383635726No Hit
GGCTGAGCCGCTTGCCTGACTTTTGATCCGACCAGTAATCACTTTGCCCGTG1360.1403595681879167No Hit
AAGCAGGGATGCATGGGTTGGCCTAACCCACAAACCATTTCATACTGAACAT1320.13623134559415442No Hit
TCACTTGGGAGGCAAAGCAGTCTGAGAAAGAAATAGGAAATGCAGAAATAGG1310.13519928994571387No Hit
TTGAACGGGCCGTGTCAACGAGGCTGCTTTACAACAGCTTTATTTATTTTTA1300.1341672342972733No Hit
TAGTACGGAGACAAATTAAATTTGGCAGAGTTTAATTGAGCAAAGGACAATT1280.13210312300039218No Hit
GGTTGGGGCAATATTGAATAAGACCTTTAGATCATTTGTGTGTGTGTGTGTG1270.13107106735195162No Hit
TATCTTGGGGTTTTGTTGTTATTAGTAATTTCTAACTGCCCTAAATTTGTCT1260.13003901170351106No Hit
TTACGAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1240.12797490040662993No Hit
CAAGCTGGGACTGCTTGATAATCACTCTCAGGTGTAAAGCTCCAAGTGTAAA1240.12797490040662993No Hit
TTCATAGGGGATGACGCGAAAGTAACCGAGACTATCAGGATCCGGAGACGGA1240.12797490040662993No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1230.12694284475818937No Hit
TAGCATGGGCAACATACCAGAATCTCTGGGACACATTCAAAGCAGTGTGTAG1210.12487873346130823No Hit
TGGCTTGGGATATGGAATAAAATAAGTATTGAAGAATTTTTCTTTGTAACAA1200.12384667781286766No Hit
TTGCACGGGATTGTCTTTCTGGTGTCCTGGGCTGTTTTGAAAAAGTTTCCAT1200.12384667781286766No Hit
TGCAATGGGGGTATTGGAGGGTGAGTAAAACCTTGAATGTGAAAACTTAAGC1180.12178256651598654No Hit
GTAAGGGAAAGTGCCTGTGTTCATTGTGGACAAAGTTATTATTTTGCAACAT1160.11971845521910542No Hit
AACGCTGGGATGAGTTGTCTGTCGACCCCTGCTGGGAGGTGTCTCCCATCAG1160.11971845521910542No Hit
TCCTAGGGGTGAGCACACATGTAACTTTCACACTCCTTGCAATTTCTAGGAG1160.11971845521910542No Hit
CACTGGGGGGTGCGAAGCCTGCCGCTGCTGCAGCGAGTCTGGCGCAGAGTGG1160.11971845521910542No Hit
TAAACTGGGAAATCAAGGTGAATGAGGTAGCATCTCTCTGTAAAGCAGAGAG1160.11971845521910542No Hit
ACACCTGAGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGAG1160.11971845521910542No Hit
GTTAGAGGGAACCTTTTGTAAGTTGAAAATATCTTAAGTCAAAAATGCATTT1150.11868639957066485No Hit
ACAAGAGGGATGGATTAATTGCCTCAAATTTGTATAAGTGATTGGCTAGTGA1140.11765434392222429No Hit
ATAAATGGGATATGGAAACTGGTTAGGTACAATACTTACAGTGCCTGTATTG1140.11765434392222429No Hit
AAGGCTGGGGTGCATGAAAAGTGACAAGCATGGGTCCCCATATTTCTACTTA1140.11765434392222429No Hit
AAAACCGGGGATCAAAACGATGCACTGCAAGGTTAACATCCAATTTTTAATA1140.11765434392222429No Hit
TGCATGGGGATAAAGGGAAAGAAAAAAGAGTTTTCTTACACAATTTTAACAT1140.11765434392222429No Hit
GTTCTGGGGATTCGTGTGTGTCTGCTTTTAAACTTGGCTGGGCTATCAGACC1140.11765434392222429No Hit
CTCAATGGGATCATAATCTCTTTGAAGAAATTAAAACTTATTTTTGTCAGTT1130.11662228827378372No Hit
TCAATGGGGAGGAATGAGATAAGAAAGGATATGATGGCAGGAAAAGAAATGC1130.11662228827378372No Hit
GTTAAAGGGCTGTGCAAACATATCATCTCTTCAAATGCTGCACACTTTGCTT1130.11662228827378372No Hit
AATTCAGGGATCTAAGCTTGTGATCATACTAAAACATTTTCACCTCTTAAAA1130.11662228827378372No Hit
TTACCTGGGATATTTATGGTAAATCTATTTTCAATTTGTGCACAGGCATTTT1130.11662228827378372No Hit
TTGCTCGGGCATTGTGTCACTGAAATGTTTAGCTTACTTTAATCTTGATATC1130.11662228827378372No Hit
AGTAGTGGGACCAATAGGAATGGATCGATAGTTGAATAATGATTTTTTATAC1120.11559023262534315No Hit
TGCCCAGGGAGCCATATCCCAGCCCCGTCTGCAGGGAGCCGGAGGCTGCTGC1120.11559023262534315No Hit
TGCTATGGGGTGCCTCTTCGGGTTGTGTAGAAGTAGGAAATGTGTCACCAAC1120.11559023262534315No Hit
AACAGTGGGAAGAGATTGACTCGGCATTGCCTTCATTCCTTAAAATTAAAAA1120.11559023262534315No Hit
AGCAATGGGGACGCTCTTCCGATCTTCTTGAGGGAAGACACAGACTGGCAAA1120.11559023262534315No Hit
TATAAAAGGGATGGAATTTCATGTGCCATAAATAAAATTGTCTTGATTTGTC1120.11559023262534315No Hit
TCTTCAGGGGCCAAAAGAAACTTTCTAAACAATGTATAGGTACACAGCAAAA1120.11559023262534315No Hit
AAAAGTGGGGAACAATAGGAGTTCCTGTGCTTGCTTAGTCATGAAAGCCAAT1120.11559023262534315No Hit
TTGCGCGGGAACAACAGGGCCAGATCCTAAAGCTCTTTCAGGGAGCTCTTCT1100.11352612132846203No Hit
GTAGGTGGGTAACTTTGTATACTTCCTGGTTTTTGAAACGTGTGACTGTGTT1100.11352612132846203No Hit
TGATATGGGTAAAAGCTTACGGAGTAAGTGGAAAAGAAAGATGCGTGCTGAA1100.11352612132846203No Hit
GCAATAGGGCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGAGCCC1100.11352612132846203No Hit
CTGAATCGGGTTGGGTAAAAGCAATAAGTCCTACACAGAATCACCAAAACCA1100.11352612132846203No Hit
TAGCTTCGGGGTGTTTTAAAATTACACTTTTTCTTAACCTGTCTAGCTGTCG1090.11249406568002147No Hit
GTCATAGGGCAAGAAGAGAAATCATGCTGTGAAGGAAATTCAGAAAATAGGA1080.1114620100315809No Hit
GCCACCGGACAGATTTTCGTGATATATTTATACACGCATGGGTTTGTAGATC1080.1114620100315809No Hit
CTCGCAGGGCACATAACCACAGATATACTCAACTGTTTCAGAATTTACTAAA1080.1114620100315809No Hit
TAGTTTGGGAGAAAATGGAATTGGAGGAACTAGTAGAATTCAGGGTAATATC1080.1114620100315809No Hit
CTTCATGGGCTGGTTCTTACAGGAACCACCTACTCTTTCAGTTTTCTGGCCC1080.1114620100315809No Hit
GTCTGCGGGATTTTGTGATCATTACAAATTCTTACACTGAATATTTTTATTT1080.1114620100315809No Hit
TACAGGGGGCACCCGTGTGACTTTCGTAACGGGGAGAGAGAGAAAAGATCTC1080.1114620100315809No Hit
TCTACTGGGGAAGTGGTGCTGTCATATTTGGTTTCTGATACTTAGGGTCTGG1070.11042995438314034No Hit
GAGTATGGGCATTCAGCACATTTTTATTGTTTTTGCAAGATGATGTTTCACT1070.11042995438314034No Hit
GCGAACGGGACTTCTTTTCTGATTTGGATGCATTGTATTTCTTTATCTTGCC1070.11042995438314034No Hit
GTCAGGGGGAACATTTATTTATTGTGTATAATAGAAGGTGTTAGATCCCATG1070.11042995438314034No Hit
GTACAGGGGTACTTGCCCTTGGCAGGTGTGGGATCCAGGCCAGTTGCATGAG1070.11042995438314034No Hit
GATCCTGGGGGCTCAAGCCTGACGCCTCTGAAAAGGCAGCAGCAGTCGCCTT1060.10939789873469978No Hit
AGATGGGTATTTCCCTAAGATCTACTAGTGATAATTCTACCTTAACTGTAAG1060.10939789873469978No Hit
CATTCCGGGATGTCCATGTCAAAATTCACTTTAGTCAGAACCAGAGTATTGA1060.10939789873469978No Hit
CTGCAAGGGGTGTGTTAAGGAGGAATTACAACGTACTTTGATTTGAATACTA1050.10836584308625921No Hit
TGAAAAGGGCGATCTTTTGCCGGGTGTAATTAGTATGAGAAATTATTTCATG1050.10836584308625921No Hit
TGCATTGGGGAAGTGGACCATGACCGGCAGGTGGTGCATGTGGAGACACTGG1050.10836584308625921No Hit
TTAAAAGGGAGCAGATATTTATAGAACATTCTATCCAAAAGCTACAGAATTT1050.10836584308625921No Hit
TAAACTGGGTTACAGTGCACTGTTCATTTAGAAGACAATTCAAGCATCACCT1050.10836584308625921No Hit
ACGTGCGGGACTGGTAATTTTTGTTTCTCAAATTTTAAGGAAATAGATAAAT1050.10836584308625921No Hit
ATACAAGGGGGGAGGCATGGGGGGTGATTAAATTATCATTTCCAAGGTGCAA1050.10836584308625921No Hit
GTAGTTGGGGTTTCTCTTAGGCAGCATATTGTTAGTTATTTTTTTAAAAAAT1040.10733378743781866No Hit
TTATGTGGGGAAACCAACAACAAACATTTTTAAAATTAAAATGGATAATTTG1040.10733378743781866No Hit
CAATCTGGGCTCCCACCTCAGCCTCCAGAGTAGCTGAAACCACAGGTGCGCA1040.10733378743781866No Hit
AACTGCGGGGCATCCTGAGACCTTGTGTCCCTGAGTCTCCTTTCTGAGTGCA1040.10733378743781866No Hit
AGTAAAGGGCAGCATCACTAAACCGATAATGCATGAAGCAACGATAAAATTA1040.10733378743781866No Hit
CTCATAGGGGTTGAAGACTTTGTTCTACTTAGAATTTCATCAAATTTTTGTC1030.10630173178937809No Hit
TTGCACGGGGGGGCTAAATTTACAGATTAACAGCATCTCAAAGCAGAACAAT1030.10630173178937809No Hit
ATTTCAGGGGGAAGTGGGAGGCCTGTGTTCTTCACTGCACCAGTCTGGAGTG1030.10630173178937809No Hit
ATTTCCGGGTGGCCTTAAACCTGTTTCAGCTTTAACAGTAGAATTTTACTTG1030.10630173178937809No Hit
TATGTGGGATTGACAGTTTGAGGCCATTACAATGTCAGCATAGATGCCAAAA1030.10630173178937809No Hit
CATGCCGGGTAGAAAACAAAAGCATCATGCATAACAACCTGCATTTTATTAG1020.1052696761409375No Hit
TACCATGGGTGATGTACGTAGATTATATTACAGTGAATTATTTTGCGTTTTT1020.1052696761409375No Hit
CTCAGCGGGACCTTCCTCTGTGTAAGTCTCCTCACCTTGAGGGGTCTTTAGT1020.1052696761409375No Hit
CGTAGTGGGCTCAGGAGTTTCTGAGAACGACTCTCGCCCAGCTTCTCCAAGT1010.10423762049249696No Hit
ATAACAGGGCTTATAAACATTCTTTAACTTTTTTGTTTGTTTGTTCTCTTTT1010.10423762049249696No Hit
ACTACTGGGTACCTATTCAGAAGAAAGTAAGTCATTATATGAAAAAGACACT1010.10423762049249696No Hit
ATTGCCGGGGAAAATTGCCACTGTTCCCTAAGAAAATGTTATTGATGGTAGG1010.10423762049249696No Hit
CTACATGGGCAGTTTAAACAAAAACAAAAACCAGACAACAACCTTGCAAGGG1000.10320556484405638No Hit
CGCTTGGGGGGCGCAGGCGCGGAATCTCCGACTTGCCATGGTCGTCTTTGTC1000.10320556484405638No Hit
CGTGGGGTCTAATACATTTTTATAAAGATATACCAGTGTTGTATATTTTTTT1000.10320556484405638No Hit
CATACGGGAGGATAGAGACTCATTCTCATCTATTTTGGTTAAAAGAAAATAA1000.10320556484405638No Hit
GTCAGAGGGACCTGAAATTGTGTAGATGGGGTATGGTGGTCTCCATCCACCA1000.10320556484405638No Hit
TGAACAGGGATCTTGTATTTCTTCTCTCTAGTATCTGAGTTCAGAAAACTTA1000.10320556484405638No Hit
CGACTGGGACCTAGATTACATTGTGTTGTTAATTCTCTGAGACTTCTAGGGA1000.10320556484405638No Hit
GCCAAGGGGCCTACTGGGAAGTGAGGAGCCCCTCTGCCCGGCCACCACCCCG990.10217350919561584No Hit
TACAATGGGACTTGTGTTTCTATACACTAATAGCAATAAACCAACCAAAAAG990.10217350919561584No Hit
TTTAGCGGGATATTTACTCAAAACATATGCCAAATTTAGTGTTGAAAGGCTC990.10217350919561584No Hit
CTTTCATGGGGGAGTGAGACTCTGTCTCAGAAAAAAAGAGGCAGCTGGGCAG990.10217350919561584No Hit
GCTAGCGGGAATGGAACAACAACGTTCACAATTGTCCAAAAAAGAAAAAAAG990.10217350919561584No Hit
AGTTGCGGGATACCCAAAGGAATATAAATCATTCTTTTATAAAGACACATGC990.10217350919561584No Hit
TGATCTGGGCTGGATATCCATATGCAGAAGAATGAAACTAGACCCCCATCTT990.10217350919561584No Hit
TGCCAAGGGGTACGCTTGCCTACCGGGAAGAGTGAAAAGATGTTAAAGAAAA990.10217350919561584No Hit
TCACAAGGGGTTTCCAAGTTGGGAATTACCTCCTACTTCTTGAAAGTTACCC990.10217350919561584No Hit
GCGATAGGGGTAAAAGGGTACTTCAAAATCCATGCCATCTTTTTTTAGTGGG980.10114145354717527No Hit
GCTACAGGGGGGCATGATCTTCAGCATAGGAAATGTCTTAGAATTTTATTAT970.10010939789873469No Hit
TGGCGTGGGGAATGTCTTTATCAGAAAAAGAGACATTTGCATACCAGATATA970.10010939789873469No Hit
AGCTGAGGCTCCAAGAAGTCACACAACTAAAATGTTAGAATTTAACTAAAAT970.10010939789873469No Hit
TGGCTTGGGGTTTAGGCCAGTTGAGTTTTGCAAAATGAGTGGTAAGGGAAAA970.10010939789873469No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCGTTGAATCCTGTCTCTTATAC970.10010939789873469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)