FastQCFastQC Report
Sat 31 Dec 2022
SRR5846158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266045
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC11330.4258678043188182No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT10240.38489729181153565No Hit
GCTAAGGGGATCCGGGAAGAGAGAGGAGAAGGGAGGGGCTTGGGAATGGCAA4220.15861978236764457No Hit
GAGCACGGGACTGGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATAC3540.13306019658328477No Hit
TTTTGGGGGGTGAAAAGAAAACCGTGCCATCTCATAGGATGTTTTACACATT3460.13005318649100717No Hit
CGTTGGGGGCTGTCTTGTAAACAAATGTTCGGTCCACTTTAATACCTTCTCT3370.12667030013719485No Hit
GCCACAGGGATGGCTTTTGATAAAAAGTAAAAAACAAAACAAAACAAAAAAA3150.11840102238343138No Hit
CTAATGCGGGGTTAAATGAATGTTTGTGAAGTGCTTTGAGATCTTAGGATGA3090.11614576481422316No Hit
CTTAGAGGGATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACTC3010.11313875472194553No Hit
ACCTTTGGGTGTTGTTCAATAGATAGCACAGTAGGATGACTACAGTTAACAA3000.11276287846041083No Hit
AAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA3000.11276287846041083No Hit
TAATTGGGGCAGGCCATCCTCTTTTATTCATCTCCTAAAAGTTTTGACTAGC2990.11238700219887612No Hit
TAAACAGGGAACATGGCTTCTCCTTTGGCATGTTTAATTGTGATATTTGACA2950.11088349715273732No Hit
CTCGCTGGGATACTATTTGAACATTCTGATACATATGGGAAATAAAACATCT2940.11050762089120261No Hit
GCGATGGGAATACTTCTCTGTAGTGTCTTTACAACTGTCTGTGTATTTGTAA2940.11050762089120261No Hit
CTGGCAAGGGGACTGTGGCAGCCATGCTACTCAAATGGAAGGCCTGCTTTCT2940.11050762089120261No Hit
TTGCCTGGGGAGAATCCTTCTCTCTCTTTGCAGTGATACCTGGAATTATAGT2890.1086282395835291No Hit
ATTTAAGGGGCACTATTTTATGTTCTTACCAGCAACCTGTAAGAGTTTCCAC2880.10825236332199441No Hit
GAGACAGGGAATACAATAAGGAAGTATATTGATTAAGATTTAGTTAGCTTTT2860.107500610798925No Hit
TGCACAGGGAGAATTAGCATGGATATACCGGGACCTCATGCAGCTTGGCAGA2830.10637298201432088No Hit
GCTAATGGGTCCTAAATATTTCAAACACGTCAGTTTCTACTTTTCCAGACAA2820.10599710575278619No Hit
ATGGTGGGGACATTACAAGAGTATAAAAATGGACATTAAATCATGGCCTTGC2820.10599710575278619No Hit
GTTTCTGGGGCTTTTCTGATTGGAGGAAAAAAGAGTTTCTAAAGTAGAACTT2820.10599710575278619No Hit
ATGAGGGATGTTGATGTGGAAGAAATGTGTTTAGGGCCAAGGCTTTGGGCTC2800.10524535322971677No Hit
TATGGTGGGCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTT2790.10486947696818207No Hit
ATTTACGGGCTTTGCATTTGTGTGCTCTTCTATTGGTAAAATGCAGAGTGGC2780.10449360070664737No Hit
AGTGGAGGGGGAAGAGTCTGAAACTTAAAATAGCAAAAATGGATAGTAGAAA2780.10449360070664737No Hit
GTCTAAGGGGTAAGGATGATTTTTTTAAAACTTTTTACATATTAGTGGTTAT2770.10411772444511268No Hit
AAATAAGGGAATTGAAAGCAGGATTTCAAAGAGATATAGTATTTGCACACCC2750.10336597192204328No Hit
TAATAGGGGATTGTGCCTCAGATTTACCAATAATGCTTTTATTTCTTATTCT2750.10336597192204328No Hit
TTGCGCGGGATTTCAGGCAAGAAGAAAGCTTGCAAAGTCATCATGATAGCAT2740.10299009566050855No Hit
GCGGCCGGGAATGGCCAAAATGCAAGTAGAAGTGGAGAAACAGAACAGGAAA2720.10223834313743915No Hit
TGCAACGGGTATTGTTAGAGATTTCTTCTTTGTTTCTTCAAGGTAAATTAAA2700.10148659061436974No Hit
AATGTAGGGCAGTCATCTCTGGCCTCCAAATTAAAATATGCTAACCTCCTCT2690.10111071435283504No Hit
GAACATGGGGATGATTTTATATCGTAAACTTTTGAAGCATATTTTTAAAAAT2690.10111071435283504No Hit
TGACTGGGGCAACCAGAAAATAATTAACAAATGACAGGCGTAAATCTTCATC2690.10111071435283504No Hit
AAACTTGGGATACAAATTGATTGGCTTATCACAAAAATCATTTCATCAGTAA2680.10073483809130035No Hit
CTTTGAGGGATCAATATTAAGTATCATCCATTTCTATGTTCTCTAGCAAAAG2680.10073483809130035No Hit
CGTAATGGGATCTCTTGATTGAATGCCCATAGGTAATTGGAAATTCTTTATT2680.10073483809130035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)