FastQCFastQC Report
Sat 31 Dec 2022
SRR5846157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265096
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC12300.4639828590397441No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT10250.38665238253312006No Hit
GCTAAGGGGATCCGGGAAGAGAGAGGAGAAGGGAGGGGCTTGGGAATGGCAA4110.1550381748498657No Hit
TGCAACGGGTATTGTTAGAGATTTCTTCTTTGTTTCTTCAAGGTAAATTAAA3640.13730874852883485No Hit
TTTTGGGGGGTGAAAAGAAAACCGTGCCATCTCATAGGATGTTTTACACATT3460.1305187554697166No Hit
CGTTGGGGGCTGTCTTGTAAACAAATGTTCGGTCCACTTTAATACCTTCTCT3430.1293870899598636No Hit
CTAATGCGGGGTTAAATGAATGTTTGTGAAGTGCTTTGAGATCTTAGGATGA3330.1256148715936868No Hit
GCCACAGGGATGGCTTTTGATAAAAAGTAAAAAACAAAACAAAACAAAAAAA3220.12146543139089234No Hit
TATGGTGGGCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTT3140.11844765669795095No Hit
CTTAGAGGGATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACTC3080.11618432567824487No Hit
GAAGCTGGGATCTGGGTAAGGTTCTCAAATTTTTCACCTCTGTGTATGTCCA3040.11467543833177415No Hit
CTCGCTGGGATACTATTTGAACATTCTGATACATATGGGAAATAAAACATCT3020.11392099465853879No Hit
TTGCCTGGGGAGAATCCTTCTCTCTCTTTGCAGTGATACCTGGAATTATAGT3000.11316655098530344No Hit
GAGACAGGGAATACAATAAGGAAGTATATTGATTAAGATTTAGTTAGCTTTT3000.11316655098530344No Hit
TGACTGGGGCAACCAGAAAATAATTAACAAATGACAGGCGTAAATCTTCATC3000.11316655098530344No Hit
TTAGCGGGATCTTCCAAAGTTTTCCTACTGCCATTACGAAAGGATTGAAAAA2990.11278932914868577No Hit
CTGGCAAGGGGACTGTGGCAGCCATGCTACTCAAATGGAAGGCCTGCTTTCT2990.11278932914868577No Hit
GAGCACGGGACTGGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATAC2980.11241210731206808No Hit
ACCTTTGGGTGTTGTTCAATAGATAGCACAGTAGGATGACTACAGTTAACAA2960.11165766363883273No Hit
CATGTTGGGTGCTCCACACGTGTTTAACCTTCTTTGTCTAAATCAGATCTAG2880.10863988894589129No Hit
AGTGGAGGGGGAAGAGTCTGAAACTTAAAATAGCAAAAATGGATAGTAGAAA2870.10826266710927362No Hit
GCGATGGGAATACTTCTCTGTAGTGTCTTTACAACTGTCTGTGTATTTGTAA2860.10788544527265594No Hit
CGTAATGGGATCTCTTGATTGAATGCCCATAGGTAATTGGAAATTCTTTATT2860.10788544527265594No Hit
TAATAGGGGATTGTGCCTCAGATTTACCAATAATGCTTTTATTTCTTATTCT2840.1071310015994206No Hit
GCTAATGGGTCCTAAATATTTCAAACACGTCAGTTTCTACTTTTCCAGACAA2780.10486767057971452No Hit
GTCTAAGGGGTAAGGATGATTTTTTTAAAACTTTTTACATATTAGTGGTTAT2780.10486767057971452No Hit
ATGAGGGATGTTGATGTGGAAGAAATGTGTTTAGGGCCAAGGCTTTGGGCTC2760.10411322690647917No Hit
GTCTGGGACTGAAGGTCCGGAAAGGCGACTTCCGGGGGCTTTGGCACCTGGC2730.10298156139662612No Hit
AAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA2730.10298156139662612No Hit
TACTTGGGGTGGTTTCATAACCTTTCCTACAATTACTTCTCCTTTTATCCCA2730.10298156139662612No Hit
TAAACAGGGAACATGGCTTCTCCTTTGGCATGTTTAATTGTGATATTTGACA2690.10147267405015542No Hit
TAGCTTGGGGCAAGAAAATTATTTTGAAAATGAATTACTGGCCTGTGCCAAG2680.10109545221353773No Hit
GTCGATGGGCATGTCTCATCCTGCCTTCTTTCTTCCTGCTGTTTGCATTTTA2660.10034100854030238No Hit
GTTTCTGGGGCTTTTCTGATTGGAGGAAAAAAGAGTTTCTAAAGTAGAACTT2660.10034100854030238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)