FastQCFastQC Report
Sat 31 Dec 2022
SRR5846156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264792
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC11740.44336686908970063No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT10290.38860690655306807No Hit
GCTAAGGGGATCCGGGAAGAGAGAGGAGAAGGGAGGGGCTTGGGAATGGCAA3840.14501948699356476No Hit
TTTTGGGGGGTGAAAAGAAAACCGTGCCATCTCATAGGATGTTTTACACATT3630.13708873379860417No Hit
TGCAACGGGTATTGTTAGAGATTTCTTCTTTGTTTCTTCAAGGTAAATTAAA3290.12424846672104899No Hit
CTTAGAGGGATTATGATCTTGCTGCAGCCACAGTGCAGCTCCACATTAACTC3260.12311550197891175No Hit
GAGACAGGGAATACAATAAGGAAGTATATTGATTAAGATTTAGTTAGCTTTT3240.12236019215082025No Hit
AGTGGAGGGGGAAGAGTCTGAAACTTAAAATAGCAAAAATGGATAGTAGAAA3220.12160488232272877No Hit
AAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA3210.12122722740868304No Hit
CGTTGGGGGCTGTCTTGTAAACAAATGTTCGGTCCACTTTAATACCTTCTCT3200.1208495724946373No Hit
ACGACCGGGGATCTAAGACTGGGAAATACATATATACATAAATATATTTACA3190.12047191758059156No Hit
TATGGTGGGCTGAGTTCACAGCTTCACCACTTGTCCATTGCGTTATTTTCTT3070.11594005861204267No Hit
GAGCACGGGACTGGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATAC3030.11442943895585969No Hit
GTCGATGGGCATGTCTCATCCTGCCTTCTTTCTTCCTGCTGTTTGCATTTTA3030.11442943895585969No Hit
GCCACAGGGATGGCTTTTGATAAAAAGTAAAAAACAAAACAAAACAAAAAAA3020.11405178404181396No Hit
CTAATGCGGGGTTAAATGAATGTTTGTGAAGTGCTTTGAGATCTTAGGATGA2990.11291881929967673No Hit
CTCGCTGGGATACTATTTGAACATTCTGATACATATGGGAAATAAAACATCT2960.1117858545575395No Hit
TAATTGGGGCAGGCCATCCTCTTTTATTCATCTCCTAAAAGTTTTGACTAGC2930.11065288981540228No Hit
ATGTTTGGGAAGTCCGATATCACAACTTAATTGTAAAAGGAAGAAATCTGAA2930.11065288981540228No Hit
TGATATGGGAGAAAAGGACATCAAAGAGTCAATCTTGCTAACATTGAGATGA2860.10800930541708209No Hit
TAATAGGGGATTGTGCCTCAGATTTACCAATAATGCTTTTATTTCTTATTCT2860.10800930541708209No Hit
TTGCCTGGGGAGAATCCTTCTCTCTCTTTGCAGTGATACCTGGAATTATAGT2850.10763165050303633No Hit
TTCAACGGGAGCCTTTCTGACCCAAACTTTCAAAAGCAAATAAACAGCATAT2830.10687634067494486No Hit
ATGGTGGGGACATTACAAGAGTATAAAAATGGACATTAAATCATGGCCTTGC2830.10687634067494486No Hit
CATGTTGGGTGCTCCACACGTGTTTAACCTTCTTTGTCTAAATCAGATCTAG2810.10612103084685338No Hit
TGACTGGGGCAACCAGAAAATAATTAACAAATGACAGGCGTAAATCTTCATC2800.10574337593280764No Hit
TACTTGGGGTGGTTTCATAACCTTTCCTACAATTACTTCTCCTTTTATCCCA2790.1053657210187619No Hit
ACCTTTGGGTGTTGTTCAATAGATAGCACAGTAGGATGACTACAGTTAACAA2750.10385510136257893No Hit
CTCACTGGGTCACATATATCTCAGTTGAATGTCACAAGAATCCCTGAAGTGA2730.10309979153448744No Hit
AGTTGGCGGAATTAGCTAAGGTAGATTATAATACATGCAGATCAGAGAGACT2660.10045620713616725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)