FastQCFastQC Report
Sat 31 Dec 2022
SRR5846152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93353
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC4330.4638308356453461No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT3470.3717073902284876No Hit
CACTGAGCAGGAAGAACCGGACCCGGCGAACCTGGAAGTGGACCATGATTTC2210.2367358306642529No Hit
CTTTTAGGGGATAATTTCTTAAATAGTAAATTGAAATAAATGATATTTTCCT1790.191745310809508No Hit
TATAATGGGGAAACAGAAGCACAAGGAATGTCATGTCAGAAACCATCCACCT1670.17889087656529518No Hit
GCCAGCGGGCGCATGGCTGCATCCTGCATCCCGAGCTCACCACCGACTCCAT1650.17674847085792636No Hit
CCAGAAGGGAGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAG1600.17139245658950436No Hit
TCTCCTGGGAAGGACAGGTGACCAGACAGCATTAGACAGGCTGTGACCTGAA1570.16817884802845115No Hit
AATAGCGGGGTATACTAGTGCTTGACCCTAGTGAATGGATAGTCTCTTTATA1570.16817884802845115No Hit
TAGAATGGGAGGGATTTGATAAGTGTTCATTACAGTTTAAAAAATGAGAGTT1570.16817884802845115No Hit
AAAACAGGGATGGGGTGGGGGCCGTGGGGGCCAGGGACACTCTCCCAGACAG1560.16710764517476676No Hit
TAATAGGGGCCTCTGTGATTTCTTCTAGAGCTTAAAAAAACCTCATAACATT1540.16496523946739794No Hit
GCACCTGGGAAAGTTTCAGTATGAAGTCTCAGTAGCCCGTGTGTGCAAAGTA1540.16496523946739794No Hit
AGTTGGGGGATACAGCGCTGGAGCCCCATTGCCTCCACCCTCCCTGAGCTGG1520.16282283376002912No Hit
CATGCTGGGATAGATGACAGCTCCATTCATGTTACTGACCCTGAAGACCTTC1500.16068042805266033No Hit
GCCATAGGGATGATAGGATGGATTCTTTCTGTATTCTGAGAGTGTACAGTAC1480.15853802234529152No Hit
ATTGTTGGGACCCATGGCAGACATTGCTAATCAATGGCAGCTCTATTTCACT1470.15746681949160712No Hit
TTACGGGGGGATCCTTTATCACGATGTTCAATTGCCTTTGAAAGAGAGTAGT1470.15746681949160712No Hit
GCCTTAGGGATCTGTGCCATTACAGAAATGATTCTTTTTATCACATCCAGAT1440.1542532109305539No Hit
CAGATAGGGCGAAAGGGACGGGAGGAAGCATCCGAAACCTAGGATTCGTCCT1420.15211080522318512No Hit
TTAGTGGGGATCCTTCGCTTGCATAATACCTTTCCTTTCTTTGGTGCCTTTT1410.1510396023695007No Hit
GATTCAGGGGAATGGGGAAAGCGGCTGAATGATGTTAACATCCCGGTAAAGA1400.1499683995158163No Hit
GCTCCTGAGATCATCTATTTAATATTCCAGGAGTTAAGAAGGAAAACAAACA1400.1499683995158163No Hit
CAACTAGGGAGAGTGACCCTCCACGAAAAGGAGAATCTTATGAATGCAGAGA1400.1499683995158163No Hit
GTATGGGGGATATGCAGGTCCTTGCATCCTACATTTCTTTAGGAAGTTACCC1390.14889719666213191No Hit
TAAGTCGGGGAAACTCTTTCCAGCTGAGATACACAAGAGACATGATTAGGTT1390.14889719666213191No Hit
TTGTAAGGGATTATGGGCAATATGTAAATAAATAAATGTAATATTTCATCCT1380.14782599380844752No Hit
TTCGTCGGGTGGGAAGCAGCAGGACAGGGATTTCTTTTTAGAGGTGCAAACT1380.14782599380844752No Hit
GTTTCAGGGCTCAATCAGAAAGATTAATGACTGTTATCAAGAACATGAACAT1370.1467547909547631No Hit
TCTGTTGGGGGTTTGGAATGATGCCACTTGATTGTGCAAATGATTTCTTATG1370.1467547909547631No Hit
CTCATGGGGGTGACCGACTTATTCTTAGATCATTGGTTTTCCAAAGGCTTTG1360.1456835881010787No Hit
ACTCATGGGAGCTCATTGAAAGATTTCTAGCATAATGAAAGATTTAACATAA1360.1456835881010787No Hit
ATCCACGGGATGCAGATTATAGGTAAAGACAGGAAGAATGATGCTTCCCCTG1360.1456835881010787No Hit
GCTGGCGGGATAGAAATGCTGACACATTCAAAGTCCTCACTTGCTTAATGGA1360.1456835881010787No Hit
AAATATGGGCTGTTTTCATGGTATTTATTTGTGATGACTATAGTCTATAGCA1360.1456835881010787No Hit
TTTCCGGGGATTTTTCCTTTTGATGATAGCAAGTGAAAAGAGATTGCTCCAA1350.14461238524739428No Hit
ACATACCGGGGTGGAAGAACTTGGAGAACCAAGACATAAATGCAGACAGGAG1350.14461238524739428No Hit
TCGATACGGGGATATTGTAAATTTCTCATTATTTATGTTATTACTTTTCTGA1350.14461238524739428No Hit
TCATAAGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT1330.1424699795400255No Hit
CTCGAAGGGATCTGGGCTATTTCTAGTTCTATTCTGTTCACTATTTCATTTA1310.14032757383265668No Hit
CAATAAGGGATTGAAAGCTTTGGAGAATGACCAAAAAGTAAGGAAAGAAGGT1300.1392563709789723No Hit
GATACCGGGGGAATTGATGATGGAAAAAATACTCCTTTTTTGGTGTTAACTT1280.1371139652716035No Hit
GTACGAGGGATACATGGCTCTGAAATATCTGACACAATGTAAACATTGCAGG1280.1371139652716035No Hit
GATAACGGGAAGGTTACAAGGCTATGATAAATGGTAGAATAGATATTCAAAT1280.1371139652716035No Hit
TCTTGCGGGAGACCATTTGTTTTCATGTGGATAATTTTAGTGCATTGCTCAC1270.13604276241791907No Hit
CTAGTTGGGACTGTGGAACAGTAATTTGTGCCCAATTTAGTGGAAGATAATG1260.13497155956423468No Hit
GAGTATGGGATTCTATGCCTGTTCTACTATTGTATTTTGGAAGCACATAACT1240.13282915385686586No Hit
ATTAGCGGGAAATAGGACCTGCTGCTTCACTAGTTGAGCAATAAGTCTGTAG1240.13282915385686586No Hit
TATTCGGGGCTTTCTTTCCGCGCCGATAGCGCTCACGCAAGCATGGTTAACG1240.13282915385686586No Hit
TGCTTTGGGGAACCATTTATCATCAAGAAAAGTGTAAGAAATTATTTTTGAG1240.13282915385686586No Hit
AATATGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1230.13175795100318147No Hit
GCCTGCGGGCGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCTTA1220.13068674814949707No Hit
GGTTCCGGGATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAG1220.13068674814949707No Hit
ATATACGGGGTGGTGGCTGATTGCCGTGTAACTATGTACATGATTGTTGGGA1220.13068674814949707No Hit
TATCAGGGGGAATGACGTGGCCATTGGAGGGCTGCAGGTCGTCCTCTCTGTA1220.13068674814949707No Hit
CGGAGGGGTCTAGAAAGAAACAAAATGTGACTTGGAGATATTAAACACACAC1210.12961554529581268No Hit
GATCAAGGGGAAATAAAAATGCAGCACTCCATTTTTTAAAAAATACTGTGTT1210.12961554529581268No Hit
CACTCAGGGTCCTGCCACTACAGATCTTTTGAAGATTGCTGGAATACTGGTG1200.12854434244212826No Hit
TGAAATGGGGTCACTTCCTTGCTGAAAACCCTTCTAAGCAGTTTTGCTTACA1200.12854434244212826No Hit
TCTAGTGGGAACCACCACCACCGTTACTACGATCAATGTTTGCGCATGTTCG1200.12854434244212826No Hit
AGCTAGGGGCAAACATCTGAAACTAAGACCTTAAGACACTATATGATATTAG1190.12747313958844386No Hit
TACTATGGGCGATCTAGGCCGGGGATTGCCTGTTTTATCCTTTGGCTTCTAT1180.12640193673475944No Hit
GGTCTAGGGATTTTGATTTTCTAGTTTTATATTTTGGACCCAATCTAGTTTT1180.12640193673475944No Hit
TACCAGGGATAAGAAGAAATTACTTTCTAAACAGTAACTGAAAATACTTAGA1180.12640193673475944No Hit
AATGTGGGGGTCTTTTTCAATGAAGCTTTGCAGATCACCATGTGGTTGAAGG1170.12533073388107507No Hit
TACACAGGGCACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA1170.12533073388107507No Hit
ATTCACGGGGTACAAGTAAATGCTTATGCTGAATCATAAATAAAATACAACA1170.12533073388107507No Hit
TGGTACGGGCACCCTCACAGGTTCTGGCCTGCCATTGACGATGGGCTGCGGC1170.12533073388107507No Hit
CAGCCAGGGGATCCTGAACTGGGTTAGGTGCCGCTGTTGCTGCTCGTGTTGA1160.12425953102739067No Hit
CAGCGAGGGAGTCCACCCCTTGGAGCTAGACATCCTGTACTTAGTCACGGGG1160.12425953102739067No Hit
GCATATGGGCTGCCTCGGCCACCCAGAGTGCTGGGATTATAGGCATGATCAC1160.12425953102739067No Hit
TTAAGGGGCACCTGTTCGAGACATAATATCCCTATGTTTGTGAGAACCTGTG1160.12425953102739067No Hit
GCTCGTGGGATTATCCATCCATTTTAGTTTTCCTCGATGATGCCCATTTTCT1150.12318832817370626No Hit
GCGTTGGATGGATGGGAATCAGTTCTTCAAAGGAGCATCATATAATTAAATG1150.12318832817370626No Hit
CTGCATGGGAGTACACCCATAGTTTCTCAACAGGTGTAGCAGATTTGTTTCT1150.12318832817370626No Hit
GACAAGGGGTCTCTTTGCGGGACTATATCTGTGTCGAATAAATGTCCTTAAT1150.12318832817370626No Hit
AAAGACGGGGATCTAAGGCATGGGATGGCACATGCAGCGCAAGTAGGTCTAC1150.12318832817370626No Hit
TAAATAGGGGAGAAAGATGAAGGGTAGGAATATGCAATTAGGAATTACACAC1140.12211712532002185No Hit
GTCTTCCAAGCTTCTCTTTCAATACATCCTGCTGCCTTTTAAAGATTTCCAA1140.12211712532002185No Hit
GGTCGGGAGCCCACGTGTCCAATTTGAATATATTTAAATGAAGAAGAAGTAT1140.12211712532002185No Hit
TCGCCTGGGATCTTAGCTCAGCAGTTCTTAGTGTGATCCGAGGCCCCTGAGA1130.12104592246633746No Hit
TAATCAGGGGAATAAAAAGGTGAGCTTTAGAAGAGGATTAAGCATTTTTTAA1120.11997471961265305No Hit
CGTACAGGGGGAATAACAAAGCCTGGTTCACAGCACATCTGTTTACAGCATG1120.11997471961265305No Hit
ACAGCTGGGAGAGTTATATTTTAGTGTACTATGTAAGTGACAGAGAATTAGC1110.11890351675896864No Hit
GCTAAAGGGACTATTCACACATGTAGGCAATTCTGGACTCACTTCCAGGATA1110.11890351675896864No Hit
TACTTCGGGATACATTCTGTATCTAGAAGTCTAAAATTTCTGCCTTTCTCCT1110.11890351675896864No Hit
CCCACAGTAAGACGTTGAAAAAAAGTCTAATAGCTCATTGTTGAATAAGAGA1110.11890351675896864No Hit
CTGAGGTGGGAGAATAGCTTGGGCCTGGGAGGCAGAGGTTGTGGTGAGCCAA1110.11890351675896864No Hit
CGTGATGGGGTTTTTATAGTTGTTGCATTGATGTTACTCATTTAGGAAAGGT1100.11783231390528423No Hit
GTCAAGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCA1100.11783231390528423No Hit
TTAGCTGGGTCTGCGAATGGGAGCTTATAAAAGAACTGCGGCTGGAACTCAT1100.11783231390528423No Hit
TACGTAGGGATTACTCTTTATTTCAAATGAAGTAGCATAAAAAATTTGAGTG1090.11676111105159985No Hit
TTCTAGGGGGATTTGAATGGGAAGAGGGAGGATGTTCCAGAGGTGTCTGGGG1090.11676111105159985No Hit
TAAGTCGGGATTGGTAAAGGTTTTCAAGGTGTCATGAAAAGATGGGGATTTA1090.11676111105159985No Hit
TTTACTGGGAGCGGGAGCTTGATATCCCTTGTCTGTAACATAGACCCCGGGT1090.11676111105159985No Hit
GCATCGAGGGAGAGGCGTGATCTGGCCTTCGACTCGCTATGTCCACTAACAA1090.11676111105159985No Hit
TTACAGCGGGATATCCAGGGACCTCACACTGACTTTCCTAAAGGAGAGAAGC1090.11676111105159985No Hit
ATGCCAGGGGTTTGGTTTCTATCCAGAAAAAATACAGGAACAGAGGAAACAA1090.11676111105159985No Hit
GAACCTGGGGAATAAACTTATTACAAGTAAAATGATTTAATTTCAAACGAAA1090.11676111105159985No Hit
ATCAGTGGGTGGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTG1080.11568990819791544No Hit
TAGTCGGGGTAAAGAAGAGAGTTTAACAACACTGAGAAATATTCCAACAGAA1080.11568990819791544No Hit
TCGAATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1080.11568990819791544No Hit
TCGTCAGGGACCCGGGAGGCGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAG1080.11568990819791544No Hit
TGCTGAGGGAATCCTGTCATTTGTGGCAATGGATGGAACTGGCAGACATTAT1070.11461870534423103No Hit
GTCATGGGGAAGAGCTCCTGAAGGAAGCACTAAGCAAGAGCCAACATTTTCA1070.11461870534423103No Hit
AAGTAGGGGATGTCTTTATTTGATCATCTATTGTACACCAGATACCTGGCTA1070.11461870534423103No Hit
ATATTAGGGAACCAGCCTAACTTTAATACACATGTATAAAGATGTTCACAGA1070.11461870534423103No Hit
CTGCGTGGGCGATCTCAAATCGGGGCTTTTGCGGGTGGCGGCGAACGCGGAG1070.11461870534423103No Hit
CTGGTTATGGGATGGTAGGGAAGCACCACCTTCATACAGATATCAAAAGCAA1070.11461870534423103No Hit
GCGCTGAGGGGGTTTTTGGAAATGTGTGTTTTCAGTATTTCTGAGATTACCA1060.11354750249054663No Hit
TAAGGGAGCATGGCTTATTGTGGGCATTTCTGCAGTGAATACATTTTCCAAA1060.11354750249054663No Hit
GACAAAGGGTAAACAATTACATTCTTCCTTGTGCCTTAGGAAATCTGTACTA1060.11354750249054663No Hit
TCTTGAGGGAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCT1060.11354750249054663No Hit
TCAAGGGGACAACACCTGGCATCTAGTCAAAGATTACCACGCTTAAAAATAA1050.11247629963686222No Hit
GCTCTTGGGCTTGGGCTTCAAGGCAGTGAAGTTACAACACCAGATTCAGACG1050.11247629963686222No Hit
TAAACTGGGGGTATTGATGACTTTTTAAAACCTGTGGGATAAACTTGCACTG1050.11247629963686222No Hit
AAGGTGGGATGATACACCGAGAGAAAAATGCAAAATATATTTGGTTCTCATT1040.11140509678317784No Hit
TTGCTGAGGGAACAAAGTAGAAGTTAATTATTCAACTTTTACCGAATGTTTA1040.11140509678317784No Hit
TCAGCAGGGCCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC1040.11140509678317784No Hit
CTCGAACCGGGGTGTTTCAGCGCTTGTGCTGATACAAAGTAAGTTATTACTT1040.11140509678317784No Hit
CATATAGGGATGATTTGTTTTAGTAAATTGTTCTTTCTAGATTTAGAAATGT1030.11033389392949343No Hit
GCATAGGGGGGGAAAGTGTGGTATATATACACAATGGAGTATTGTTTAGTCC1030.11033389392949343No Hit
CATAGCGGGGCACATTTGACTCCTGAGATGAATTTTTTGTGGTCATAATCAA1030.11033389392949343No Hit
CACACAGGGTAAGTGAAAGTTTTCAGGAAAATTACTATCCATTTGCTTATGT1030.11033389392949343No Hit
TCTAAAGGGAAGTCAAACATATTTAGTTGAGTGAATACATTCAGGTATTCAG1030.11033389392949343No Hit
GCACATGGAGAGGCCAAGCCAACAACAGCAGCAGGGTCTGTGCTGGTTCCTG1020.10926269107580902No Hit
TTGCAGGGGAAATGAAGGTTGGATGGATGGATACTGACAATGGTGGCAGGCA1010.10819148822212461No Hit
AGCTATGGGGGCCCCTTTGAGATGCCAGGTTTCTTTTTTATCTAGAAATGGC1010.10819148822212461No Hit
TACTTAGGGAACCATTATGAACAATTTGGGGGAAATATGAAATGGAATAAAA1010.10819148822212461No Hit
AGGCTTGGGATTGAAGTGGAGATTCCATAATTATGTCAGTGTTTAAAGGTTT1010.10819148822212461No Hit
GCTACAGGGAGTTGAGAAAAGGACTTTCTGATTATAGATATTAAGACCCTAT1010.10819148822212461No Hit
AGTTTAGGGGACAATGAGCAGCTGCCATCTTGGGGGATTTCATTCTGTGGTT1000.10712028536844023No Hit
TATAAAGGGCGATCTTGAAGCGGGCATTAAAGACGATCTAGAAGATATAAAG1000.10712028536844023No Hit
CCCTGCGCCATCAATGAGAAGATCTGCTCTTAGTAGAGCCCTGCTTTCCCGC990.10604908251475582No Hit
GCCAATGGGTTAGGCGCTACCTGTACCTCCTTTGTGCCTGCCTCTAGTAGAG990.10604908251475582No Hit
AAGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.10604908251475582No Hit
ACACATGGGGAATACTTGGGAATTTATAGAACTGTTTTTATTAGCCTTTTAA990.10604908251475582No Hit
TTCGTAGGGGTCTCTAAAGTTGCTACAAGGGAATGAGTAATTGAAAGGTGAG990.10604908251475582No Hit
CCAATCGGGGAAGATTACAGTCCTGATGACAAACCCTTGGTCACACTGATTA990.10604908251475582No Hit
TAGTAAGGGGAATTTCGAAAGAAATAATTGGCAAGATAATGAGAAAAGAAAA990.10604908251475582No Hit
TTGTCGGGGGTTATTTCCTGCCTTTCCGAGTCCTGCAGTGGGCTGCCCTGTA990.10604908251475582No Hit
CACTTGGGGGATCTCTTAACTCCACATGCCTGGGAGACCTAGAGATCCTAGC980.10497787966107142No Hit
TACATATGGGACTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACCC980.10497787966107142No Hit
TCAAAACGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG980.10497787966107142No Hit
ATATATGGGGATATCCTAAGATAGTGTGTGACCTGAACCTAATCACAAGGAA970.10390667680738701No Hit
GTTAGCGGATCCTTAACATGGGAGACTTTTTCTTTGGATACCTTCAATATTT970.10390667680738701No Hit
GAGTTTGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG970.10390667680738701No Hit
TTGGCTGGGATTTATCTAACTTCTGTGTCAAAATTAGCGACCTGCTATGGCA960.10283547395370263No Hit
CGTAAAGGGAATATAAACCAGATTCAAATAAATATTGTGATAAAATATACAG960.10283547395370263No Hit
AACGCGGGGCTGTTCCTTGCAATATAGTACTTCGTGTTTGTAATCAATGTGT960.10283547395370263No Hit
AGCGACGGGGTGGGGGAGTTACCAACACTTTTGTAAAAGGCCAGATAGTAAA960.10283547395370263No Hit
TCACATGGGCCTGAGCAAGTGGGACCTTGGTATACACATCACCTGTTCTTTC960.10283547395370263No Hit
GTGGGACTGTATAACCAGCCATCAGATACCAAGGTGTACCATGAGAACATCA960.10283547395370263No Hit
TGGGCTGGGGAGTCCCTCACAGCTATCTTTGTCTCGCAGGCCTTCCTGGGCT950.10176427110001822No Hit
AACAGCGGAGACAGGAGGGTGAGACATCCTCCATGATTTTTTCCATCCCCTA950.10176427110001822No Hit
TCAGCAGGGAGGCCTCAGCCCTGACAGCATCCCACCATTTCCAGGATGTTCC950.10176427110001822No Hit
ATCGCTGGGACTACAGTGATATAAAAACCAGCAGTTTTTTACACTAAATTTT950.10176427110001822No Hit
GCCTGTGGGGTAAGAGATTTCTTTATTTTCTTGGAAATTCTCTGGAACAGTA950.10176427110001822No Hit
TACTTGGGTATTGAAGACCTTCAAGTAGAAGCGGATTTTCCTGTCTATTTTG950.10176427110001822No Hit
ATTAAAGGGAGTACAAGTTGACATGGAAAGAGAACAATGTTCTCACTGACAT950.10176427110001822No Hit
TTTCAGGGTAGACGAGATTTGTTCAACACTGCAATTGTTTGTTAGATGTAAA940.10069306824633381No Hit
GTAGAATCTACCTTAGGTAGATGATCCCTAGACATACGTTGGTTTTGAGGGC940.10069306824633381No Hit
GCATAAGGGAAAGAACTCACACCGGAGAAGAGCCTTATGAATGTAAGCAATG940.10069306824633381No Hit
TGTCTGGGCAGAAGCTGTTATAGAGCATGAAACATAATAAGCATTCAATAAG940.10069306824633381No Hit
GTACAGGGGAAGTACGATAAGAAGAAAGTTCAGCTCCTTAACTAATTAAAAT940.10069306824633381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)