FastQCFastQC Report
Sat 31 Dec 2022
SRR5846149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258172
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC11650.45124955456052557No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT9580.37107044915792575No Hit
TAGATAGGGATAGCCAGTTTTCTTCCCTCCTTGCTATTAAAGCCAGAGCGGT5380.208388206312071No Hit
TCTGTGGGGGAAAAAAACCCAAAACACTGGCATCTGAGTAGGAATATGAAAA4540.1758517577429001No Hit
AATGCAGGGGATTCTGAGCATTTGAAAAAGTGAGATAAAAGGAACATGAGGT4530.17546441906945756No Hit
TCAATCGGGCTTGAACTGGTAACTGATTAGGTTGAACTTATGGAAGATTTGT4480.173527725702245No Hit
CAATTAGGGATTACAGGCATGGTGTGAAACTTGATGTTTGACCTGTGATCAA4420.17120369366158994No Hit
ATCGACGGGACTGAAAATATTTATGCTTATATTGGCTTTATGTAATCTGTAC4410.17081635498814743No Hit
AAGCGAGGGCGGGGTTTTTTGGTGGGGTGTAGGTATGGGGGGATAACATCTG4330.16771764560060734No Hit
TGATTCCGGGGTGATTAGGATGACTGTTACAGGCTTAGCTTTGTGTGAAAAC4290.1661682909068373No Hit
CTAGCCGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT4220.16345692019273972No Hit
AGCGGAGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA4210.1630695815192972No Hit
TACTCGGGGGTGATATCAATGGCGCAGGCTTCACTTCTGCTGTGGCGGTGAC4210.1630695815192972No Hit
CTAAAGGGGGGTCTCGACACCTGGACTAGCCGGGTTGTATTTGGAAACGCGG4190.16229490417241219No Hit
TCAACCGGGATACCTCTATTTTGCATCTAGTGATTTCTCATATTATCTTGTA4160.16113288815208465No Hit
ATTAAGGGGACAAACAAACAAACACCAACAAAAAAGCCCCCGCGAAAGGACC4130.15997087213175712No Hit
TAAAATGGGATGATGCCTGCAGTATAAATCAGATTTTACAGAGAGCTTGATC4120.1595835334583146No Hit
CAATGCGGGACTTGCAGTAATTTTCAAAGTCCTTAAACTTGAAGCCATGTTT4100.15880885611142959No Hit
CGCGAAGGGAAGACTGTTTATGCTTAAACACTCAGCTTCACATACCACTTCC4040.15648482407077452No Hit
AATACAGGGATATGATACCTATTCACAGCTCGAAAACATACTTGAGTTATGA4010.155322808050447No Hit
GCTAATGGAAATCAGATTGGAAAACCCATTCTGACTCCACATACTGCTACAA3990.15454813070356196No Hit
TCTCTTGGGATGTGCTAACAGTCATTTTGCTGACTGTTCTGGAGACTCCCAT3990.15454813070356196No Hit
GTGCGTGGGAATTCACTGTTGTGAGAATATTCCTCGTCACAGCAAAAACACT3960.15338611468323443No Hit
AGCTATGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA3950.15299877600979192No Hit
TAACCGGGAACTACTTAAGTGACTGACTATGATGCTGTGCAAGCAGTTATAT3930.1522240986629069No Hit
GCTTAAGGGAAAGAAGGATAAGAAAACTCTAACATTTATGTTGTTAAATGGT3930.1522240986629069No Hit
AGCACTGGGATGTGGTATTTTTAAAGACTGATCTTAGACCAAGTCAAATTCC3910.15144942131602188No Hit
TATCTTGGGATTGTGTGCATGTGTATGTATGTGTGTGCATGCGTATGCACAC3910.15144942131602188No Hit
AATAACGGGATCTTAGGCTCTTTCAAAACGTCCTGTAGTTGCCTGATGTAGA3910.15144942131602188No Hit
TCTAAGGGGCGACGCTCTTCCGATCTTTTCCGGGGGGCTTTCCTCAACATTT3900.15106208264257937No Hit
AAAAATGGGACTGGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAACTC3820.14796337325503928No Hit
GCGTTTGGGAGATGGGCCATCTGAATGGACGTTGAAACCACACAGAATGTTG3800.14718869590815425No Hit
ATATTAGGGGGAAATGATTTGTATTTATACTCAACAATGTACATGTACCAAA3770.14602667988782672No Hit
TCATGAGGGCACAGGATTTGCTGTCAATAAGGTTTCTCTGGAAATACTAGAA3760.1456393412143842No Hit
GCCAAGGGTCTGTGCATTTTGAAAGGAGATTTCTAAAAATAATTAAGGTGCC3760.1456393412143842No Hit
AAGCTTGGGGTAATTATGAGGGAAGTTAAATATCTTTCTCTTGAATTGTCAG3750.1452520025409417No Hit
AGCTATGGGATGTTTCCTCCTGGATTGCTCTCTGGAAAGGTTTAGTTGTTTA3710.14370264784717166No Hit
GCAAATGGGGACTGAAGAGGGAAAAGGAGTGAGAAGACAAAGCCGTCAAAGC3680.14254063182684412No Hit
TTAATCGGGATAATTTTCTAGACATGCATCTGTTGGTTGTAGGATGGCCCCA3670.1421532931534016No Hit
TAATTAGGGGGAGTGATGCTGACGTTACTATTTCCTATCTTATCCCAGGTAG3640.14099127713307408No Hit
GTTGCCGGGATTCCTGTAAGTTTCTTAACTGCATTTTGATGAATTCACATTA3630.14060393845963157No Hit
AGTGGTGGGAGCACTCTCAGGATATTCATTTTGAGCTATTAGATAAAATCAA3620.14021659978618906No Hit
CTACACGGGAAGGAATATAAATGATATTCTTGTTCAGGTGCTCAGAGCAATA3620.14021659978618906No Hit
GTTGATGGGAACTCCATTTCAAAAGATTATTTTGATACCCTTAATTGTAATC3600.13944192243930403No Hit
CAATGTGGGATTGTAAATTAACAAAATGTCAAAATTTAAAAACATTCAGTTC3580.138667245092419No Hit
CTGCTAGGGGCTCAAACCAATGGAACAATTTGCTGAGGCCTGGAAGCAACCC3560.137892567745534No Hit
CAGCCGGGGCGCCACTGCACTCCAGCCTGGGCCACAAGAGCAAAACTCCGTC3550.13750522907209148No Hit
GTCTTCGGGAGATTTGGAAGCTACCTGTACATCTGCAATATTGTGTTTTTAA3520.13634321305176395No Hit
GAATGTGGGCTACACAATGGATTGAAGGGAGGGCAAGAAAAGTTAGTAGGAG3500.13556853570487892No Hit
TACGCTGGGACTCATGTTCTGTGTTCTCTAACATCAGTCTACATGTGGCGTT3490.1351811970314364No Hit
ACTATAGGGATCACACATCACATGTGCCTGCTATTCATCTCTCAAGCTTTGC3480.1347938583579939No Hit
TACTACGGGCATGATTCATCGATCCGAGATGCCCACAGGTGCTATCCTTAGT3470.1344065196845514No Hit
ATACATGGGACAAGGAATTGAGAAGAGAGGATACTTAGTTGCTCCATGTTAA3460.13401918101110888No Hit
CGCGCCGGGATTGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACA3450.13363184233766637No Hit
TATCGTAGGGAATGAATATAGACACAAAAATCCTCAAAAAAAATGCCAGCAA3450.13363184233766637No Hit
GCAGCGGGGAGACCTACAGTTACTGGCTACTTGCATTTGTCAGTTTAGAGAA3430.13285716499078132No Hit
CTAGCCGGGAATTTATTCTAAACTTTCCAGCTGTGAAACTGATGCTTTTAAA3420.13246982631733883No Hit
TTACTAGGGATCTGGAATATTTTGTGGTCATATTTGAGCAATATATATTGGG3420.13246982631733883No Hit
CAGAATGGGTAACCGGCCCTTTCCCAATTCGCCCTAAGACTTTGGGGGTGGC3400.1316951489704538No Hit
AGCACTGGGAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCAT3390.1313078102970113No Hit
AAAGGTGGGCCTGTGGCGTCTCAAAAAAAAGTTTCATCGTCCCGTCATTGTT3390.1313078102970113No Hit
TAGCTAGGGAATCTCAGTGCTTTGGGAGGCCAAGGCAGGAGGGTCACTTGAA3380.13092047162356876No Hit
CATCTTGGGAGCACATCATAAGGACTAAGTTATCAGGAGCTATTTTAACTAT3370.13053313295012628No Hit
CGCACTGGGATTCTCCGAGGCCCTGATGTAGAAACTTCCCCATTGGGTGCAC3360.13014579427668377No Hit
GCGCTAGGGCATTTGACAAAATCCAGCATCCTTTTGTGATTAAAACCCTCAG3360.13014579427668377No Hit
TTTAGGGGGAACTTGTGTGTATTTCTTTATAATTCTATCTTAAACAACATAT3350.12975845560324126No Hit
GCAAAAGGGCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTC3330.1289837782563562No Hit
TCATAAGGGACCTGTCTGGTATTGACCTCATGTTTTAAGCATTAGAAAATGA3330.1289837782563562No Hit
GCAAATGGGCAACCCAAATGCTCAACAATGATAGACTGGATAAAGAAAATGT3320.12859643958291372No Hit
GTAGCAGGGACAAAAACTGTCCATTCCCACCCCTTTGCTTTGCCATTTGCAA3320.12859643958291372No Hit
TAAAAGGGGGGCCCTGCTCTGCGGCCTCCTTTGTCCCATAACCTGAAGTTAA3310.1282091009094712No Hit
CAGTATGGGAGAGAAGACGGTGCTGTTTAAAAAAAATACTCCATTACAAATA3310.1282091009094712No Hit
TGACCCGGGATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGGTCTATT3280.12704708488914365No Hit
GCGCGAGGGCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACC3250.12588506886881615No Hit
CAATACAAGAATATTGACAACAAAAGGACAAAGTAGTTATTGCACAGGACGG3240.12549773019537364No Hit
AGACGCGGGACTCATTGCCTCGGTTGGTGTCTAGCACCTAGTAGGTTCTCAA3240.12549773019537364No Hit
TTCTTTGGGCATAAAATCACTTCCAAATCCTGTTGCTTATGTTGGGTTAAAT3240.12549773019537364No Hit
ACATCAGGGAGTTCTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGT3230.1251103915219311No Hit
CAGGCAAGGAAACAAAGCGAGGTTAAGGTTAGACTCTTGGGAATCAGCTAGT3220.1247230528484886No Hit
TTCAAAGGGATACGGGCAAGTTTATAAGGTAAGTACATTGAGTCTGAGAGTG3200.12394837550160358No Hit
ATATAGGGGAGAGCTAAAGTCATAGTGACATTAATGATTAACATGTTCCTTT3180.12317369815471856No Hit
TAGCAAGGGACCCCTGAATTGGCAGGAATTGTCAAAGGAAATAGAGATGCAA3180.12317369815471856No Hit
TCTATAGGGAAATGACATTATCAAAATCCAGGCTTTTATTCGGGCAAACAAA3160.12239902080783352No Hit
AATAATGGGAATTTACCAGAATTCATTTTAATTTTTACTTGTAATATCAGCA3160.12239902080783352No Hit
AATTGTGGGAGCTGTTGCTGTTATTATCATCATTATTATTATTTCCACCACC3160.12239902080783352No Hit
AATAGGGGGATGTATCTAGTGGTTACTAGAACTCAGGATCACACAATATAGT3150.12201168213439102No Hit
TACTAGGGGCATGTGGCCCTGACCCCAGCCATCCCAGCACTACTGCCCACTA3120.12084966611406349No Hit
CATAGCAGGTGCCCAACAAGCATGAGTACCTTTCTCACCTCAGAATGGAGCA3100.12007498876717847No Hit
GCATGAGGGATATGTTTTTTAAATTCAAAAAAGAATATGAAATTATACCTTT3080.11930031142029345No Hit
GAGTCAGGGATTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCA3070.11891297274685093No Hit
ACAAATGGGAATGGAGTTCTTGTGTTAACAAATTACGTGCTATATGATTTTT3050.11813829539996591No Hit
AATGCAGGGGTTTCTCCTGGTAGGCTAAAGCCCTGGCCGCTTGTCTGACTCA3040.11775095672652341No Hit
AACAATGGGATACCTAGAAGTGGAATTGTTGTTATAGAGTGTGTGCTTTTTC3040.11775095672652341No Hit
GCTAACGGGATATCTCTTCCTTGACTGATAAAGTAATACTAGCTATTTGATA3030.11736361805308089No Hit
ATATACGGGCAGATATGGGGCAACTAATAAGGAATCTTCTGCAGTAATCCAG3020.11697627937963838No Hit
AACACCGGGAATCTTGGAGTTCTTGTTTGGCTCTACTCTTAGCCAACCCCAC3010.11658894070619585No Hit
CGACAGGGACACTGGGGCTAGGGAAGTAGGTGATGCCAGCCCTCAAGTCTGT3000.11620160203275336No Hit
TCAGCAGGGGTATTTAGTATGCTCCTCAACTAATGATGGAATTGCATCCAGA3000.11620160203275336No Hit
GTTGCCGGAAGTCAGCTGTTAATCTTACAAAACATAGGTGCTCAAAAAATAA2990.11581426335931086No Hit
TTTCATGGGAAAGTTGAAGTTCAGGTCTTGCCATTGCCATTACAGACAAATT2980.11542692468586833No Hit
ACTATCGGGATAGCATGTTCTCCATTTGCAGTCTACAAAGGGGAATTTTATT2970.11503958601242582No Hit
GGAAGTTGGGGGTATCCATGTGATTCAGAGGCTGAGACCTGTGCATGCTCAT2970.11503958601242582No Hit
CGTATAGGGGATCTAGTGGGGGGTGCAAATCAATTGATGTGATACATCATAT2960.1146522473389833No Hit
CATCATGGGATCTCCCTGTGTCTTAATATGGTCTTCCCTCTCTGCATGTCTG2900.11232821529832825No Hit
GTGATGGGTATACCAGTCCCAAGAATGGTGATATTAAGAATCCCACTTACAA2900.11232821529832825No Hit
CTGACAGGGGGTGGCCGAAGTGGGACGCGCCGAGCCGGAGGCTGCAGGATGA2880.11155353795144322No Hit
TAACACGGGGTTAAGGGAGGTAGACGTTTAACAATTACTAACCACTTTGGTT2870.11116619927800071No Hit
CTGCTTGGGAAGGTGAGGAGAGAAGACAAAAAATCACTGAAATACAGCTAAA2870.11116619927800071No Hit
TATGCCGGGAATAATACAGAGCCAGGCAGACTTTGGTTTGAGTCCAGGTGTT2860.11077886060455819No Hit
CATTACGGGCAGTTTACTCAAAAACATAATAAATTAATTGAGGACTTAATGT2860.11077886060455819No Hit
CGAACTGGGACCTCTTTAAAGAACTTATCTCTAAATACAGCCACATTCTGTG2830.10961684458423067No Hit
CGTAGCGGGCTTGTTACTGTATGTGCCCCTAATATTAGGATTTTCTTCACAG2820.10922950591078816No Hit
TTAAGGGGGTGCTTATTGGATTCCAGTACAAACGCCACCAAAGCCAGCCCAC2810.10884216723734563No Hit
CTTGCAGGGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAAT2800.10845482856390314No Hit
TGGGCGGGACTCGAGGAGAACGAGCTCACCAGCCTGGCAGAGGACTTGCTGG2800.10845482856390314No Hit
CATGCCGGGGTATAAAATACTTTTAAAAGGGTGAATTTAGCATTTAACAGTT2780.10768015121701811No Hit
CAATCAGGGCGTTTAAAATTTTGCTTTATATGCCCTCTATCACTTAGCATAA2770.1072928125435756No Hit
TTTTTGGGGCAGTGACTATGGCTTCAAAATTAATTTATTAATCTTTTCCTTT2770.1072928125435756No Hit
TCAGCCGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT2770.1072928125435756No Hit
GTGGCAGGGACATATTCTTTTTTTTCAAACAAACCTACTTTTCAAAATTGTT2760.10690547387013308No Hit
ATAAAAGGGGAAAGTCATAGCAATTCTACTATATTTGATGTCTTCAACTTGA2760.10690547387013308No Hit
ATGTGGGGCAGTAATGCAAACTTGCTTTAAAGGAACCAAAGGCATTGCCAAG2740.10613079652324806No Hit
TCTTATGGGCAAAGGATTGCAGGACAACAACTACAGCGTTGTGTACTGTGGG2740.10613079652324806No Hit
AAATCTGGGGCTGCTATATGGTGATATGTTTGTGTTGGCTCTGAAAAGAGAA2740.10613079652324806No Hit
TAGCAAGGGATGACAGTCCATCAGACAGATCTGGCCCACTACCCATTTTTGT2720.10535611917636305No Hit
GTTCTGAGGGGGGTGGTGCCTACTTCCACTGTAAGAAAGAATCTTGGTGGAT2710.10496878050292052No Hit
ATACACCGGGGGAGTGCTTATGAATGACTAAATAAATGGGTGTGTTCTTTCC2690.1041941031560355No Hit
AAAGCAGGGGGCTGTGCATTCTATTTTCTACATAGCACTGAATCTGAGTAAC2680.103806764482593No Hit
TTCTGAGGACTAATCTGCTTCCTATTACTGTAAATTAGTTTGCGTTTCCTAG2670.10341942580915049No Hit
TATGGCGGGACACGGGACAGTTGCTGGCATGTCTTCATTGGCTATATAAAAT2660.10303208713570797No Hit
GCGCATGGGGCCTAAACCCTGACCTGGAGGGACAGCCATTGAGGATGAGAGG2650.10264474846226547No Hit
TCTCATGGGTCATTTCAAAATGTTTAGTCTTTAAAGCGGTTACCTAAGAATA2650.10264474846226547No Hit
GCTTGGGTGGAGACTTCTGTCTCAACAAGAACAACAAAGTCAACTAGCTAAA2650.10264474846226547No Hit
TAGAGAGGGTTCCTGATGTCGGTGCAGGAGCTGAAGGTGAGTGGAGTGCTGC2640.10225740978882294No Hit
TAGCCACGGGAGTGGTGAAGAATACAATGACTACAAATAGAGTTTGGCACCA2630.10187007111538045No Hit
TCTTCCGGGAAGTGGGATTTACCTCAGGAGTGCGAAGTTTTTTCAACATATG2620.10148273244193794No Hit
ACTATCGGGATAATCTCCCCAAATATTTTTCTAAAGATTCAAGTTAAATATA2610.10109539376849541No Hit
AAGCATGGGATTATTTCCAGATGAATTTATCTTCTGAATAAAGGAATTTATT2600.10070805509505291No Hit
GTATGGGGGAGAACCAGTATCCAGACAGAAGAAGAGAAACTGAGGCCAGCAT2600.10070805509505291No Hit
TTGGTTGGGATGGGAATTATATGTAATTCTCAACCGCAAGGATTTTTTTTTT2590.10032071642161039No Hit
ATGCCGGGACTCCAGTTTCATGTCAACTACTGGGACAAATATTAACCACCCT2590.10032071642161039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)