FastQCFastQC Report
Sat 31 Dec 2022
SRR5846148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258177
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC11050.4280009450880597No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT9630.37299991866045384No Hit
TAGATAGGGATAGCCAGTTTTCTTCCCTCCTTGCTATTAAAGCCAGAGCGGT5290.20489819000143314No Hit
AATGCAGGGGATTCTGAGCATTTGAAAAAGTGAGATAAAAGGAACATGAGGT5090.19715156656092528No Hit
AAGCGAGGGCGGGGTTTTTTGGTGGGGTGTAGGTATGGGGGGATAACATCTG5070.19637690421687448No Hit
TCTGTGGGGGAAAAAAACCCAAAACACTGGCATCTGAGTAGGAATATGAAAA4950.19172893015256973No Hit
TCAATCGGGCTTGAACTGGTAACTGATTAGGTTGAACTTATGGAAGATTTGT4720.18282031319598568No Hit
TACTCGGGGGTGATATCAATGGCGCAGGCTTCACTTCTGCTGTGGCGGTGAC4560.1766230144435794No Hit
TAACCGGGAACTACTTAAGTGACTGACTATGATGCTGTGCAAGCAGTTATAT4500.17429902741142703No Hit
ATTAAGGGGACAAACAAACAAACACCAACAAAAAAGCCCCCGCGAAAGGACC4480.17352436506737626No Hit
TAAAATGGGATGATGCCTGCAGTATAAATCAGATTTTACAGAGAGCTTGATC4440.1719750403792747No Hit
AGCTATGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA4430.17158770920724928No Hit
CAATTAGGGATTACAGGCATGGTGTGAAACTTGATGTTTGACCTGTGATCAA4170.16151709873458905No Hit
GCTTAAGGGAAAGAAGGATAAGAAAACTCTAACATTTATGTTGTTAAATGGT4160.16112976756256367No Hit
GTGCGTGGGAATTCACTGTTGTGAGAATATTCCTCGTCACAGCAAAAACACT4150.16074243639053826No Hit
ATCGACGGGACTGAAAATATTTATGCTTATATTGGCTTTATGTAATCTGTAC4120.1595804428744621No Hit
AGCACTGGGATGTGGTATTTTTAAAGACTGATCTTAGACCAAGTCAAATTCC4100.1588057805304113No Hit
CAATGCGGGACTTGCAGTAATTTTCAAAGTCCTTAAACTTGAAGCCATGTTT4090.15841844935838592No Hit
AGTGGTGGGAGCACTCTCAGGATATTCATTTTGAGCTATTAGATAAAATCAA4080.1580311181863605No Hit
TATCGTAGGGAATGAATATAGACACAAAAATCCTCAAAAAAAATGCCAGCAA4080.1580311181863605No Hit
AATACAGGGATATGATACCTATTCACAGCTCGAAAACATACTTGAGTTATGA4070.15764378701433512No Hit
GCGTTTGGGAGATGGGCCATCTGAATGGACGTTGAAACCACACAGAATGTTG4040.15648179349825894No Hit
AAAAATGGGACTGGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAACTC4010.15531979998218276No Hit
AGCGGAGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA4000.15493246881015738No Hit
CTAGCCGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT4000.15493246881015738No Hit
CGCGAAGGGAAGACTGTTTATGCTTAAACACTCAGCTTCACATACCACTTCC3980.15415780646610658No Hit
TCATGAGGGCACAGGATTTGCTGTCAATAAGGTTTCTCTGGAAATACTAGAA3980.15415780646610658No Hit
AAGCTTGGGGTAATTATGAGGGAAGTTAAATATCTTTCTCTTGAATTGTCAG3970.1537704752940812No Hit
TGATTCCGGGGTGATTAGGATGACTGTTACAGGCTTAGCTTTGTGTGAAAAC3960.1533831441220558No Hit
TAATTAGGGGGAGTGATGCTGACGTTACTATTTCCTATCTTATCCCAGGTAG3950.1529958129500304No Hit
GCTAATGGAAATCAGATTGGAAAACCCATTCTGACTCCACATACTGCTACAA3950.1529958129500304No Hit
GCCAAGGGTCTGTGCATTTTGAAAGGAGATTTCTAAAAATAATTAAGGTGCC3920.15183381943395421No Hit
CTACACGGGAAGGAATATAAATGATATTCTTGTTCAGGTGCTCAGAGCAATA3880.15028449474585265No Hit
TATCTTGGGATTGTGTGCATGTGTATGTATGTGTGTGCATGCGTATGCACAC3880.15028449474585265No Hit
ATACATGGGACAAGGAATTGAGAAGAGAGGATACTTAGTTGCTCCATGTTAA3830.14834783888572567No Hit
AGCTATGGGATGTTTCCTCCTGGATTGCTCTCTGGAAAGGTTTAGTTGTTTA3820.14796050771370028No Hit
TCAACCGGGATACCTCTATTTTGCATCTAGTGATTTCTCATATTATCTTGTA3790.1467985141976241No Hit
AATAACGGGATCTTAGGCTCTTTCAAAACGTCCTGTAGTTGCCTGATGTAGA3790.1467985141976241No Hit
TACTACGGGCATGATTCATCGATCCGAGATGCCCACAGGTGCTATCCTTAGT3740.14486185833749715No Hit
AAAGGTGGGCCTGTGGCGTCTCAAAAAAAAGTTTCATCGTCCCGTCATTGTT3730.14447452716547174No Hit
CAGTATGGGAGAGAAGACGGTGCTGTTTAAAAAAAATACTCCATTACAAATA3720.14408719599344635No Hit
CATCTTGGGAGCACATCATAAGGACTAAGTTATCAGGAGCTATTTTAACTAT3700.14331253364939556No Hit
ATATTAGGGGGAAATGATTTGTATTTATACTCAACAATGTACATGTACCAAA3680.1425378713053448No Hit
TTTAGGGGGAACTTGTGTGTATTTCTTTATAATTCTATCTTAAACAACATAT3670.14215054013331938No Hit
CAGCCGGGGCGCCACTGCACTCCAGCCTGGGCCACAAGAGCAAAACTCCGTC3670.14215054013331938No Hit
GCAAATGGGGACTGAAGAGGGAAAAGGAGTGAGAAGACAAAGCCGTCAAAGC3660.141763208961294No Hit
TTAATCGGGATAATTTTCTAGACATGCATCTGTTGGTTGTAGGATGGCCCCA3630.1406012154452178No Hit
GAATGTGGGCTACACAATGGATTGAAGGGAGGGCAAGAAAAGTTAGTAGGAG3600.13943922192914163No Hit
CTAAAGGGGGGTCTCGACACCTGGACTAGCCGGGTTGTATTTGGAAACGCGG3570.13827722841306544No Hit
CTAGCCGGGAATTTATTCTAAACTTTCCAGCTGTGAAACTGATGCTTTTAAA3560.13788989724104006No Hit
GCAGCGGGGAGACCTACAGTTACTGGCTACTTGCATTTGTCAGTTTAGAGAA3540.13711523489698926No Hit
GTTGCCGGGATTCCTGTAAGTTTCTTAACTGCATTTTGATGAATTCACATTA3520.1363405725529385No Hit
AATAGGGGGATGTATCTAGTGGTTACTAGAACTCAGGATCACACAATATAGT3480.13479124786483693No Hit
ATATAGGGGAGAGCTAAAGTCATAGTGACATTAATGATTAACATGTTCCTTT3470.13440391669281151No Hit
TACGCTGGGACTCATGTTCTGTGTTCTCTAACATCAGTCTACATGTGGCGTT3460.13401658552078613No Hit
TACTAGGGGCATGTGGCCCTGACCCCAGCCATCCCAGCACTACTGCCCACTA3450.13362925434876075No Hit
CAGAATGGGTAACCGGCCCTTTCCCAATTCGCCCTAAGACTTTGGGGGTGGC3420.13246726083268456No Hit
CGCGCCGGGATTGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACA3410.13207992966065915No Hit
CGTATAGGGGATCTAGTGGGGGGTGCAAATCAATTGATGTGATACATCATAT3410.13207992966065915No Hit
CATAGCAGGTGCCCAACAAGCATGAGTACCTTTCTCACCTCAGAATGGAGCA3400.13169259848863377No Hit
TCTAAGGGGCGACGCTCTTCCGATCTTTTCCGGGGGGCTTTCCTCAACATTT3400.13169259848863377No Hit
GTAGCAGGGACAAAAACTGTCCATTCCCACCCCTTTGCTTTGCCATTTGCAA3360.1301432738005322No Hit
ACATCAGGGAGTTCTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGT3350.1297559426285068No Hit
TCATAAGGGACCTGTCTGGTATTGACCTCATGTTTTAAGCATTAGAAAATGA3340.1293686114564814No Hit
ACAAATGGGAATGGAGTTCTTGTGTTAACAAATTACGTGCTATATGATTTTT3310.12820661794040522No Hit
TAAAAGGGGGGCCCTGCTCTGCGGCCTCCTTTGTCCCATAACCTGAAGTTAA3300.12781928676837984No Hit
TCTCTTGGGATGTGCTAACAGTCATTTTGCTGACTGTTCTGGAGACTCCCAT3300.12781928676837984No Hit
CAATACAAGAATATTGACAACAAAAGGACAAAGTAGTTATTGCACAGGACGG3280.12704462442432904No Hit
CAATGTGGGATTGTAAATTAACAAAATGTCAAAATTTAAAAACATTCAGTTC3270.12665729325230365No Hit
CGCACTGGGATTCTCCGAGGCCCTGATGTAGAAACTTCCCCATTGGGTGCAC3270.12665729325230365No Hit
TTTCATGGGAAAGTTGAAGTTCAGGTCTTGCCATTGCCATTACAGACAAATT3270.12665729325230365No Hit
AGACGCGGGACTCATTGCCTCGGTTGGTGTCTAGCACCTAGTAGGTTCTCAA3250.12588263090825286No Hit
GTTGCCGGAAGTCAGCTGTTAATCTTACAAAACATAGGTGCTCAAAAAATAA3230.1251079685642021No Hit
TAGCTAGGGAATCTCAGTGCTTTGGGAGGCCAAGGCAGGAGGGTCACTTGAA3200.1239459750481259No Hit
ACTATAGGGATCACACATCACATGTGCCTGCTATTCATCTCTCAAGCTTTGC3180.12317131270407511No Hit
ACTATCGGGATAGCATGTTCTCCATTTGCAGTCTACAAAGGGGAATTTTATT3180.12317131270407511No Hit
GCGCGAGGGCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACC3170.12278398153204972No Hit
GTTGATGGGAACTCCATTTCAAAAGATTATTTTGATACCCTTAATTGTAATC3160.12239665036002433No Hit
AGCACTGGGAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCAT3140.12162198801597354No Hit
TTACTAGGGATCTGGAATATTTTGTGGTCATATTTGAGCAATATATATTGGG3130.12123465684394814No Hit
CGACAGGGACACTGGGGCTAGGGAAGTAGGTGATGCCAGCCCTCAAGTCTGT3120.12084732567192276No Hit
CTGACAGGGGGTGGCCGAAGTGGGACGCGCCGAGCCGGAGGCTGCAGGATGA3120.12084732567192276No Hit
TGGGCGGGACTCGAGGAGAACGAGCTCACCAGCCTGGCAGAGGACTTGCTGG3120.12084732567192276No Hit
GTCTTCGGGAGATTTGGAAGCTACCTGTACATCTGCAATATTGTGTTTTTAA3120.12084732567192276No Hit
TTCTTTGGGCATAAAATCACTTCCAAATCCTGTTGCTTATGTTGGGTTAAAT3120.12084732567192276No Hit
ATACACCGGGGGAGTGCTTATGAATGACTAAATAAATGGGTGTGTTCTTTCC3080.11929800098382118No Hit
GCATGAGGGATATGTTTTTTAAATTCAAAAAAGAATATGAAATTATACCTTT3060.1185233386397704No Hit
GGAAGTTGGGGGTATCCATGTGATTCAGAGGCTGAGACCTGTGCATGCTCAT3060.1185233386397704No Hit
AATAATGGGAATTTACCAGAATTCATTTTAATTTTTACTTGTAATATCAGCA3050.118136007467745No Hit
CTGCTAGGGGCTCAAACCAATGGAACAATTTGCTGAGGCCTGGAAGCAACCC3040.11774867629571961No Hit
CAGGCAAGGAAACAAAGCGAGGTTAAGGTTAGACTCTTGGGAATCAGCTAGT3030.11736134512369421No Hit
TCTTCCGGGAAGTGGGATTTACCTCAGGAGTGCGAAGTTTTTTCAACATATG3010.11658668277964343No Hit
AACACCGGGAATCTTGGAGTTCTTGTTTGGCTCTACTCTTAGCCAACCCCAC3010.11658668277964343No Hit
TAGCAAGGGACCCCTGAATTGGCAGGAATTGTCAAAGGAAATAGAGATGCAA3010.11658668277964343No Hit
ATAAAAGGGGAAAGTCATAGCAATTCTACTATATTTGATGTCTTCAACTTGA3000.11619935160761803No Hit
TATGCCGGGAATAATACAGAGCCAGGCAGACTTTGGTTTGAGTCCAGGTGTT2990.11581202043559263No Hit
GCAAATGGGCAACCCAAATGCTCAACAATGATAGACTGGATAAAGAAAATGT2980.11542468926356725No Hit
ATAGTTGGGACCAGCAAGTGATTCTTAAAGGAGACTTTAATTACTTGGAAGA2980.11542468926356725No Hit
GCAAAAGGGCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTC2970.11503735809154185No Hit
GCGCTAGGGCATTTGACAAAATCCAGCATCCTTTTGTGATTAAAACCCTCAG2960.11465002691951647No Hit
TCTTATGGGCAAAGGATTGCAGGACAACAACTACAGCGTTGTGTACTGTGGG2960.11465002691951647No Hit
CTGCTTGGGAAGGTGAGGAGAGAAGACAAAAAATCACTGAAATACAGCTAAA2950.11426269574749107No Hit
AATTGTGGGAGCTGTTGCTGTTATTATCATCATTATTATTATTTCCACCACC2930.11348803340344028No Hit
TCTATAGGGAAATGACATTATCAAAATCCAGGCTTTTATTCGGGCAAACAAA2920.11310070223141488No Hit
CAATCAGGGCGTTTAAAATTTTGCTTTATATGCCCTCTATCACTTAGCATAA2920.11310070223141488No Hit
GAGTCAGGGATTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCA2900.1123260398873641No Hit
GTGATGGGTATACCAGTCCCAAGAATGGTGATATTAAGAATCCCACTTACAA2890.1119387087153387No Hit
TTAAGGGGGTGCTTATTGGATTCCAGTACAAACGCCACCAAAGCCAGCCCAC2890.1119387087153387No Hit
AATAGCGGGGTAAGCATCTGGGTTCAAGTCCAGGTGTTGTCACTCACCAACT2880.11155137754331332No Hit
AAGCATGGGATTATTTCCAGATGAATTTATCTTCTGAATAAAGGAATTTATT2880.11155137754331332No Hit
TCAGCCGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT2870.11116404637128792No Hit
TCTTGGAGGGGATGTGAAAATTTTAGGCAACCTTCTAAATCTTTTTCACCAT2860.11077671519926252No Hit
ATGTGGGGCAGTAATGCAAACTTGCTTTAAAGGAACCAAAGGCATTGCCAAG2860.11077671519926252No Hit
TCAGCAGGGGTATTTAGTATGCTCCTCAACTAATGATGGAATTGCATCCAGA2860.11077671519926252No Hit
TAGAGAGGGTTCCTGATGTCGGTGCAGGAGCTGAAGGTGAGTGGAGTGCTGC2840.11000205285521174No Hit
GCTAACGGGATATCTCTTCCTTGACTGATAAAGTAATACTAGCTATTTGATA2840.11000205285521174No Hit
TTCAAAGGGATACGGGCAAGTTTATAAGGTAAGTACATTGAGTCTGAGAGTG2840.11000205285521174No Hit
ATATACGGGCAGATATGGGGCAACTAATAAGGAATCTTCTGCAGTAATCCAG2820.10922739051116095No Hit
TTGGTTGGGATGGGAATTATATGTAATTCTCAACCGCAAGGATTTTTTTTTT2800.10845272816711017No Hit
CATTACGGGCAGTTTACTCAAAAACATAATAAATTAATTGAGGACTTAATGT2800.10845272816711017No Hit
GGGTTTGGGTTCAGCAAAAAATACCAGAGATTTTCATATGGATTGTGCTGAA2790.10806539699508477No Hit
TAACACGGGGTTAAGGGAGGTAGACGTTTAACAATTACTAACCACTTTGGTT2790.10806539699508477No Hit
GCGGTTGGGTACATGGCATGGTTCTCTCCTGGAAACATCCTAAAGATGGTAA2790.10806539699508477No Hit
TCTACAGGGAGTGATATCACATTATAGTTGTTTCAGATGTCTCAAAATATTA2780.10767806582305937No Hit
TTTTTGGGGCAGTGACTATGGCTTCAAAATTAATTTATTAATCTTTTCCTTT2780.10767806582305937No Hit
CATCATGGGATCTCCCTGTGTCTTAATATGGTCTTCCCTCTCTGCATGTCTG2770.10729073465103399No Hit
CTTGCAGGGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAAT2770.10729073465103399No Hit
AAAGCAGGGGGCTGTGCATTCTATTTTCTACATAGCACTGAATCTGAGTAAC2760.10690340347900859No Hit
CATGCCGGGGTATAAAATACTTTTAAAAGGGTGAATTTAGCATTTAACAGTT2750.10651607230698319No Hit
GCTCACGGGAATTTTGAAGGTCTTCACAGTGAGATTTTTATAGAAACCCAAT2750.10651607230698319No Hit
TAGCCACGGGAGTGGTGAAGAATACAATGACTACAAATAGAGTTTGGCACCA2740.10612874113495781No Hit
TATGGCGGGACACGGGACAGTTGCTGGCATGTCTTCATTGGCTATATAAAAT2730.10574140996293241No Hit
TGCCAAGGGGAAGCCAGTTCCTAAATGTTCTAGAGCAGCAGTCAGCAAACTC2730.10574140996293241No Hit
AGTTACGGGATCCAGGTAAAACACTGGCCCAAGATTTGGTAAAGAGATTTCA2700.10457941644685623No Hit
ATATGGGTTATTTTTTCACTTCAAAAATGATTCTAAAGTACATTAGAGAGAA2690.10419208527483084No Hit
GAATATGGGAGGCTGAGCCTTCTCTGGGTTCCCACTTCCCATGCTGATTCCC2680.10380475410280544No Hit
CGTAGCGGGCTTGTTACTGTATGTGCCCCTAATATTAGGATTTTCTTCACAG2680.10380475410280544No Hit
ATAAGTGGGGGTGGGGCCAGGGTGAGGCAAGTGTGCCACTTGCCTTATGCAC2680.10380475410280544No Hit
ACTTTTGGGGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAAC2680.10380475410280544No Hit
TTGTCTGGGTACCTCAATAAAGGTAAATACAGGGGCCGTTATGAAAGCAAGT2670.10341742293078005No Hit
TGCGCAGGGTCTACATGGGGGCCTAGTGCTCATCTTGAATTACAAGTTTATT2670.10341742293078005No Hit
CTATTGACGGGATTTTAAACAATGGTTTGAATAGTTATGGGATGAAGTAGAC2630.10186809824267848No Hit
ACAACAGGGCGCGAAGGGCAGTGGTAACCATAACAAGTACCAACAATGAACT2620.10148076707065308No Hit
AATGCAGGGGTTTCTCCTGGTAGGCTAAAGCCCTGGCCGCTTGTCTGACTCA2620.10148076707065308No Hit
TTATATGGGATGTAAAATGATTTATACAATATGCACAGTTTCATGCCTTTCA2620.10148076707065308No Hit
TTCTGAGGACTAATCTGCTTCCTATTACTGTAAATTAGTTTGCGTTTCCTAG2590.1003187735545769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)