FastQCFastQC Report
Sat 31 Dec 2022
SRR5846147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257314
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC11220.4360431224107511No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT10150.39445968738583986No Hit
TCTGTGGGGGAAAAAAACCCAAAACACTGGCATCTGAGTAGGAATATGAAAA4930.19159470530169365No Hit
AATGCAGGGGATTCTGAGCATTTGAAAAAGTGAGATAAAAGGAACATGAGGT4850.18848566343067225No Hit
TCAATCGGGCTTGAACTGGTAACTGATTAGGTTGAACTTATGGAAGATTTGT4520.175660865712709No Hit
AAGCGAGGGCGGGGTTTTTTGGTGGGGTGTAGGTATGGGGGGATAACATCTG4490.17449497501107594No Hit
TAGATAGGGATAGCCAGTTTTCTTCCCTCCTTGCTATTAAAGCCAGAGCGGT4470.1737177145433206No Hit
CTAGCCGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT4380.17022004243842154No Hit
TCAACCGGGATACCTCTATTTTGCATCTAGTGATTTCTCATATTATCTTGTA4300.16711100056740014No Hit
CGCGAAGGGAAGACTGTTTATGCTTAAACACTCAGCTTCACATACCACTTCC4210.16361332846250107No Hit
CAATTAGGGATTACAGGCATGGTGTGAAACTTGATGTTTGACCTGTGATCAA4170.16205880752699037No Hit
TATCGTAGGGAATGAATATAGACACAAAAATCCTCAAAAAAAATGCCAGCAA4160.1616701772931127No Hit
AATACAGGGATATGATACCTATTCACAGCTCGAAAACATACTTGAGTTATGA4140.16089291682535733No Hit
GCTTAAGGGAAAGAAGGATAAGAAAACTCTAACATTTATGTTGTTAAATGGT4100.15933839588984666No Hit
TGATTCCGGGGTGATTAGGATGACTGTTACAGGCTTAGCTTTGTGTGAAAAC4070.1581725051882136No Hit
TAAAATGGGATGATGCCTGCAGTATAAATCAGATTTTACAGAGAGCTTGATC4070.1581725051882136No Hit
TAACCGGGAACTACTTAAGTGACTGACTATGATGCTGTGCAAGCAGTTATAT4060.15778387495433593No Hit
ATTAAGGGGACAAACAAACAAACACCAACAAAAAAGCCCCCGCGAAAGGACC4040.1570066144865806No Hit
GCTAATGGAAATCAGATTGGAAAACCCATTCTGACTCCACATACTGCTACAA4030.15661798425270293No Hit
AGCGGAGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA4030.15661798425270293No Hit
AGCACTGGGATGTGGTATTTTTAAAGACTGATCTTAGACCAAGTCAAATTCC4030.15661798425270293No Hit
CAGCCGGGGCGCCACTGCACTCCAGCCTGGGCCACAAGAGCAAAACTCCGTC4010.15584072378494757No Hit
AAAAATGGGACTGGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAACTC4000.1554520935510699No Hit
ATCGACGGGACTGAAAATATTTATGCTTATATTGGCTTTATGTAATCTGTAC3960.1538975726155592No Hit
GTTGCCGGGATTCCTGTAAGTTTCTTAACTGCATTTTGATGAATTCACATTA3930.15273168191392616No Hit
AAAGGTGGGCCTGTGGCGTCTCAAAAAAAAGTTTCATCGTCCCGTCATTGTT3920.1523430516800485No Hit
TCTAAGGGGCGACGCTCTTCCGATCTTTTCCGGGGGGCTTTCCTCAACATTT3810.14806811910739406No Hit
AATAACGGGATCTTAGGCTCTTTCAAAACGTCCTGTAGTTGCCTGATGTAGA3810.14806811910739406No Hit
AGCTATGGGATGTTTCCTCCTGGATTGCTCTCTGGAAAGGTTTAGTTGTTTA3800.1476794888735164No Hit
CAGTATGGGAGAGAAGACGGTGCTGTTTAAAAAAAATACTCCATTACAAATA3800.1476794888735164No Hit
TACTCGGGGGTGATATCAATGGCGCAGGCTTCACTTCTGCTGTGGCGGTGAC3780.14690222840576106No Hit
GCGTTTGGGAGATGGGCCATCTGAATGGACGTTGAAACCACACAGAATGTTG3740.14534770747025036No Hit
GTGCGTGGGAATTCACTGTTGTGAGAATATTCCTCGTCACAGCAAAAACACT3710.14418181676861735No Hit
CAATGCGGGACTTGCAGTAATTTTCAAAGTCCTTAAACTTGAAGCCATGTTT3700.14379318653473966No Hit
CTACACGGGAAGGAATATAAATGATATTCTTGTTCAGGTGCTCAGAGCAATA3690.143404556300862No Hit
CATCTTGGGAGCACATCATAAGGACTAAGTTATCAGGAGCTATTTTAACTAT3670.14262729583310663No Hit
AGCTATGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA3580.13912962372820756No Hit
CGCGCCGGGATTGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACA3550.13796373302657453No Hit
AGTGGTGGGAGCACTCTCAGGATATTCATTTTGAGCTATTAGATAAAATCAA3520.1367978423249415No Hit
CAGAATGGGTAACCGGCCCTTTCCCAATTCGCCCTAAGACTTTGGGGGTGGC3520.1367978423249415No Hit
GCGCTAGGGCATTTGACAAAATCCAGCATCCTTTTGTGATTAAAACCCTCAG3520.1367978423249415No Hit
CTAGCCGGGAATTTATTCTAAACTTTCCAGCTGTGAAACTGATGCTTTTAAA3510.13640921209106385No Hit
TTAATCGGGATAATTTTCTAGACATGCATCTGTTGGTTGTAGGATGGCCCCA3480.13524332138943082No Hit
ATACATGGGACAAGGAATTGAGAAGAGAGGATACTTAGTTGCTCCATGTTAA3470.13485469115555312No Hit
TCTCTTGGGATGTGCTAACAGTCATTTTGCTGACTGTTCTGGAGACTCCCAT3470.13485469115555312No Hit
CAATGTGGGATTGTAAATTAACAAAATGTCAAAATTTAAAAACATTCAGTTC3460.13446606092167546No Hit
ACTATAGGGATCACACATCACATGTGCCTGCTATTCATCTCTCAAGCTTTGC3460.13446606092167546No Hit
AATAATGGGAATTTACCAGAATTCATTTTAATTTTTACTTGTAATATCAGCA3450.1340774306877978No Hit
CTAAAGGGGGGTCTCGACACCTGGACTAGCCGGGTTGTATTTGGAAACGCGG3450.1340774306877978No Hit
TACTAGGGGCATGTGGCCCTGACCCCAGCCATCCCAGCACTACTGCCCACTA3450.1340774306877978No Hit
GCAAATGGGGACTGAAGAGGGAAAAGGAGTGAGAAGACAAAGCCGTCAAAGC3440.13368880045392012No Hit
GTTGCCGGAAGTCAGCTGTTAATCTTACAAAACATAGGTGCTCAAAAAATAA3440.13368880045392012No Hit
CTGCTTGGGAAGGTGAGGAGAGAAGACAAAAAATCACTGAAATACAGCTAAA3430.13330017022004245No Hit
GTAGCAGGGACAAAAACTGTCCATTCCCACCCCTTTGCTTTGCCATTTGCAA3430.13330017022004245No Hit
CAGGCAAGGAAACAAAGCGAGGTTAAGGTTAGACTCTTGGGAATCAGCTAGT3410.1325229097522871No Hit
CGTATAGGGGATCTAGTGGGGGGTGCAAATCAATTGATGTGATACATCATAT3410.1325229097522871No Hit
ATATTAGGGGGAAATGATTTGTATTTATACTCAACAATGTACATGTACCAAA3400.13213427951840942No Hit
GTTGATGGGAACTCCATTTCAAAAGATTATTTTGATACCCTTAATTGTAATC3400.13213427951840942No Hit
TCAGCAGGGGTATTTAGTATGCTCCTCAACTAATGATGGAATTGCATCCAGA3390.13174564928453172No Hit
GCCAAGGGTCTGTGCATTTTGAAAGGAGATTTCTAAAAATAATTAAGGTGCC3390.13174564928453172No Hit
CAATACAAGAATATTGACAACAAAAGGACAAAGTAGTTATTGCACAGGACGG3370.1309683888167764No Hit
CATTACGGGCAGTTTACTCAAAAACATAATAAATTAATTGAGGACTTAATGT3370.1309683888167764No Hit
GAATGTGGGCTACACAATGGATTGAAGGGAGGGCAAGAAAAGTTAGTAGGAG3360.13057975858289872No Hit
GCAGCGGGGAGACCTACAGTTACTGGCTACTTGCATTTGTCAGTTTAGAGAA3360.13057975858289872No Hit
ACTATCGGGATAGCATGTTCTCCATTTGCAGTCTACAAAGGGGAATTTTATT3350.13019112834902105No Hit
TAATTAGGGGGAGTGATGCTGACGTTACTATTTCCTATCTTATCCCAGGTAG3340.12980249811514336No Hit
GTCTTCGGGAGATTTGGAAGCTACCTGTACATCTGCAATATTGTGTTTTTAA3330.1294138678812657No Hit
TACTACGGGCATGATTCATCGATCCGAGATGCCCACAGGTGCTATCCTTAGT3330.1294138678812657No Hit
AATTGTGGGAGCTGTTGCTGTTATTATCATCATTATTATTATTTCCACCACC3290.127859346945755No Hit
AAGCTTGGGGTAATTATGAGGGAAGTTAAATATCTTTCTCTTGAATTGTCAG3280.12747071671187732No Hit
ACAAATGGGAATGGAGTTCTTGTGTTAACAAATTACGTGCTATATGATTTTT3260.12669345624412195No Hit
TATCTTGGGATTGTGTGCATGTGTATGTATGTGTGTGCATGCGTATGCACAC3240.12591619577636662No Hit
GCAAATGGGCAACCCAAATGCTCAACAATGATAGACTGGATAAAGAAAATGT3230.12552756554248895No Hit
TCATGAGGGCACAGGATTTGCTGTCAATAAGGTTTCTCTGGAAATACTAGAA3210.12475030507473359No Hit
TTTCATGGGAAAGTTGAAGTTCAGGTCTTGCCATTGCCATTACAGACAAATT3210.12475030507473359No Hit
CTGCTAGGGGCTCAAACCAATGGAACAATTTGCTGAGGCCTGGAAGCAACCC3190.12397304460697825No Hit
ATACACCGGGGGAGTGCTTATGAATGACTAAATAAATGGGTGTGTTCTTTCC3180.12358441437310057No Hit
TAGCTAGGGAATCTCAGTGCTTTGGGAGGCCAAGGCAGGAGGGTCACTTGAA3170.1231957841392229No Hit
AATAGGGGGATGTATCTAGTGGTTACTAGAACTCAGGATCACACAATATAGT3150.12241852367146755No Hit
TTGGTTGGGATGGGAATTATATGTAATTCTCAACCGCAAGGATTTTTTTTTT3140.12202989343758988No Hit
TTAAGGGGGTGCTTATTGGATTCCAGTACAAACGCCACCAAAGCCAGCCCAC3140.12202989343758988No Hit
TGGGCGGGACTCGAGGAGAACGAGCTCACCAGCCTGGCAGAGGACTTGCTGG3120.12125263296983452No Hit
AGCACTGGGAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCAT3110.12086400273595684No Hit
TTACTAGGGATCTGGAATATTTTGTGGTCATATTTGAGCAATATATATTGGG3110.12086400273595684No Hit
TAGCAAGGGACCCCTGAATTGGCAGGAATTGTCAAAGGAAATAGAGATGCAA3110.12086400273595684No Hit
TTTAGGGGGAACTTGTGTGTATTTCTTTATAATTCTATCTTAAACAACATAT3100.12047537250207917No Hit
GCTAACGGGATATCTCTTCCTTGACTGATAAAGTAATACTAGCTATTTGATA3100.12047537250207917No Hit
CGACAGGGACACTGGGGCTAGGGAAGTAGGTGATGCCAGCCCTCAAGTCTGT3090.1200867422682015No Hit
TTCTTTGGGCATAAAATCACTTCCAAATCCTGTTGCTTATGTTGGGTTAAAT3090.1200867422682015No Hit
AGACGCGGGACTCATTGCCTCGGTTGGTGTCTAGCACCTAGTAGGTTCTCAA3060.11892085156656848No Hit
TAAAAGGGGGGCCCTGCTCTGCGGCCTCCTTTGTCCCATAACCTGAAGTTAA3050.1185322213326908No Hit
AACACCGGGAATCTTGGAGTTCTTGTTTGGCTCTACTCTTAGCCAACCCCAC3030.11775496086493543No Hit
CGCACTGGGATTCTCCGAGGCCCTGATGTAGAAACTTCCCCATTGGGTGCAC3030.11775496086493543No Hit
CATAGCAGGTGCCCAACAAGCATGAGTACCTTTCTCACCTCAGAATGGAGCA3020.11736633063105778No Hit
GCGCGAGGGCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACC3010.11697770039718011No Hit
CTGACAGGGGGTGGCCGAAGTGGGACGCGCCGAGCCGGAGGCTGCAGGATGA3000.11658907016330242No Hit
TCATAAGGGACCTGTCTGGTATTGACCTCATGTTTTAAGCATTAGAAAATGA3000.11658907016330242No Hit
AATAGCGGGGTAAGCATCTGGGTTCAAGTCCAGGTGTTGTCACTCACCAACT2990.11620043992942475No Hit
CGAACTGGGACCTCTTTAAAGAACTTATCTCTAAATACAGCCACATTCTGTG2990.11620043992942475No Hit
TCTATAGGGAAATGACATTATCAAAATCCAGGCTTTTATTCGGGCAAACAAA2980.11581180969554707No Hit
GCGGTTGGGTACATGGCATGGTTCTCTCCTGGAAACATCCTAAAGATGGTAA2980.11581180969554707No Hit
TCATTGGGGGAGTACTCTCTTAAATGCTTTCATAAGCAGGACAGATTGTAAA2970.1154231794616694No Hit
ATATAGGGGAGAGCTAAAGTCATAGTGACATTAATGATTAACATGTTCCTTT2950.11464591899391405No Hit
TAGCCACGGGAGTGGTGAAGAATACAATGACTACAAATAGAGTTTGGCACCA2950.11464591899391405No Hit
TCTTGGAGGGGATGTGAAAATTTTAGGCAACCTTCTAAATCTTTTTCACCAT2930.11386865852615871No Hit
TACGCTGGGACTCATGTTCTGTGTTCTCTAACATCAGTCTACATGTGGCGTT2930.11386865852615871No Hit
CAATCAGGGCGTTTAAAATTTTGCTTTATATGCCCTCTATCACTTAGCATAA2920.11348002829228102No Hit
GCATGAGGGATATGTTTTTTAAATTCAAAAAAGAATATGAAATTATACCTTT2910.11309139805840336No Hit
TAGAGAGGGTTCCTGATGTCGGTGCAGGAGCTGAAGGTGAGTGGAGTGCTGC2910.11309139805840336No Hit
GCTCACGGGAATTTTGAAGGTCTTCACAGTGAGATTTTTATAGAAACCCAAT2900.11270276782452567No Hit
ATAGTTGGGACCAGCAAGTGATTCTTAAAGGAGACTTTAATTACTTGGAAGA2890.112314137590648No Hit
AAGCATGGGATTATTTCCAGATGAATTTATCTTCTGAATAAAGGAATTTATT2890.112314137590648No Hit
AAAGCAGGGGGCTGTGCATTCTATTTTCTACATAGCACTGAATCTGAGTAAC2880.11192550735677033No Hit
ATGTGGGGCAGTAATGCAAACTTGCTTTAAAGGAACCAAAGGCATTGCCAAG2860.11114824688901498No Hit
TGGGTAGGGTGCCTAGAACATTGTAGAATCAGTAGAGACTAAATTGGCATGG2850.1107596166551373No Hit
GAATATGGGAGGCTGAGCCTTCTCTGGGTTCCCACTTCCCATGCTGATTCCC2830.10998235618738196No Hit
TATGGCGGGACACGGGACAGTTGCTGGCATGTCTTCATTGGCTATATAAAAT2800.10881646548574894No Hit
TAACACGGGGTTAAGGGAGGTAGACGTTTAACAATTACTAACCACTTTGGTT2800.10881646548574894No Hit
TGCGCAGGGTCTACATGGGGGCCTAGTGCTCATCTTGAATTACAAGTTTATT2790.10842783525187125No Hit
ATATACGGGCAGATATGGGGCAACTAATAAGGAATCTTCTGCAGTAATCCAG2770.1076505747841159No Hit
GTAGCGGGATGTGGCCTTGAGGATAACCTGCACTGTAATAGCCTAAACACAA2760.10726194455023823No Hit
CTTGCAGGGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAAT2760.10726194455023823No Hit
GCAAAAGGGCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTC2760.10726194455023823No Hit
CTAGCGGGACTTGATGGTACAGTAAATTTACTGGCATTTTTCTCCTTAAAAA2760.10726194455023823No Hit
AGTTACGGGATCCAGGTAAAACACTGGCCCAAGATTTGGTAAAGAGATTTCA2750.10687331431636056No Hit
AATGCAGGGGTTTCTCCTGGTAGGCTAAAGCCCTGGCCGCTTGTCTGACTCA2740.10648468408248288No Hit
TCTTCCGGGAAGTGGGATTTACCTCAGGAGTGCGAAGTTTTTTCAACATATG2720.10570742361472751No Hit
TCAACTGGGGTTCCAGCGTGATTGTAAGTTTTCCAAGGCCTCCCCAGTCATA2700.10493016314697219No Hit
GAGCCGGGGCCAGCCTAATCAAATGTCATAATTGTTGTACCTATTGAAAGTT2680.10415290267921683No Hit
AACAATGGGATACCTAGAAGTGGAATTGTTGTTATAGAGTGTGTGCTTTTTC2680.10415290267921683No Hit
ATGCCGGGACTCCAGTTTCATGTCAACTACTGGGACAAATATTAACCACCCT2680.10415290267921683No Hit
GCGCATGGGGCCTAAACCCTGACCTGGAGGGACAGCCATTGAGGATGAGAGG2670.10376427244533917No Hit
CATCATGGGATCTCCCTGTGTCTTAATATGGTCTTCCCTCTCTGCATGTCTG2660.10337564221146148No Hit
TCTCATGGGTCATTTCAAAATGTTTAGTCTTTAAAGCGGTTACCTAAGAATA2660.10337564221146148No Hit
AAAGCCGGGTCTGTAAAACAAATGTAACTATATTTATTCCTTAGTACTATTT2660.10337564221146148No Hit
GGGTTTGGGTTCAGCAAAAAATACCAGAGATTTTCATATGGATTGTGCTGAA2650.10298701197758381No Hit
ACATCAGGGAGTTCTGTTACTTAGGCTGTTAGATCCAAGTTGATTTTTGTGT2650.10298701197758381No Hit
GGAAGTTGGGGGTATCCATGTGATTCAGAGGCTGAGACCTGTGCATGCTCAT2650.10298701197758381No Hit
TTCAGCGGGATATATGTGAAGATAAAACTTTTGCTTACTCCTTAGCTAATGA2620.10182112127595079No Hit
TGACCCGGGATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGGTCTATT2620.10182112127595079No Hit
GTGATGGGTATACCAGTCCCAAGAATGGTGATATTAAGAATCCCACTTACAA2620.10182112127595079No Hit
GACGCAGGGATGTGACAGTGACTTTGTGCTGAAATTTCAGCTATTCCAGATA2610.10143249104207311No Hit
CGTAGCGGGCTTGTTACTGTATGTGCCCCTAATATTAGGATTTTCTTCACAG2600.10104386080819544No Hit
TCTACAGGGAGTGATATCACATTATAGTTGTTTCAGATGTCTCAAAATATTA2600.10104386080819544No Hit
ATAAAAGGGGAAAGTCATAGCAATTCTACTATATTTGATGTCTTCAACTTGA2600.10104386080819544No Hit
TGTCGCGGGGGAAGTTTATTTGACTAGGTACCTTCTTTATAGAATTCATGAG2600.10104386080819544No Hit
TGCCAAGGGGAAGCCAGTTCCTAAATGTTCTAGAGCAGCAGTCAGCAAACTC2600.10104386080819544No Hit
AAGCAGGGAAGGAATCAAAATGGGTTAGTTATCTTTGTTTGTTTGAGGCAGA2590.10065523057431774No Hit
GAGTCAGGGATTTGGTGAAGATGCACTTGCAAATGTCAGCATTGAGAAGCCA2580.10026660034044008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)