FastQCFastQC Report
Sat 31 Dec 2022
SRR5846119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220775
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT10160.46019703317857547No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC8890.40267240403125354No Hit
CTCTATGGGACTTCCTTAGGAGTCATTCATTTGTATATTTGACACTTTTGCT4330.1961272789038614No Hit
GCAACGGGGCGATCTAATTTGGGGGGGATTCACACAATACTTGACAATCACA3530.15989129203940664No Hit
CGAAACGGGACAATGGAATATTATTCAGCTTTAAAAAAGAAGGAAATCTTGT3530.15989129203940664No Hit
GAAAACGGGCGTGGGCGGACGACTGGAAGGCCATTCTGGACAGCAAGCGGCC3480.15762654286037822No Hit
TGTACAGGGTGTGGGGAATGTGGGAAATCCTTTAGCCATAGCTCCAACCTTA3400.15400294417393273No Hit
ATTAGCGGGAAATATAAAAAGTAACATTTTATTGAAATGTATTTCTTAATTA3350.15173819499490432No Hit
TAACTAGGGCTGCTGTTTCTTTTATTTTCTATTGCATTGATTTTTATTCTAA3250.14720869663684746No Hit
GCAAACGGCTTCCGATCTGAAAGTGGGCAGTCCGTGGAGAGAACAAAACCCA3240.14675574680104178No Hit
GCAACAGGGCTGGTTCTTAAAGCCAATCAATAAACAGATTTTGAAAAAGCTT3220.14584984712943042No Hit
AAACACGGGAGAAACAGAAAAAAGCAATCCTAAAATTCATATGGAAACACAA3210.14539689729362473No Hit
TTTCTGGGTGACGTGTGGGTCTTTTGTTAAAGAGAGCAGTGTTAGAGTCAAT3200.14494394745781905No Hit
TGAGTCGGGAGGACCTCCTGGAACTGACTTCTTCTGTCAGGAGGACTGGTTT3180.14403804778620768No Hit
TAGACTGGGTCTTGATGTGAATTCCACTGTTACCTGACCATGTATATTCAGA3150.1426791982787906No Hit
AACAATGGGTCGAGATCACAAGTTGCATGTTGACTTTAGGAACGTGAAGCTC3130.14177329860717924No Hit
GGTATGGGGCGACCTAAAGCAGGGACGGAGGTCTTGTGTATCTGTGGCAACA3110.14086739893556788No Hit
ATGTGCGGGACTTCAAATGCCAGCTGAAATCCAGACGGTGATTGGCCTCAAA3110.14086739893556788No Hit
ACCTCAGGGAACTGGTTTTTATTACATTAAGTTATACCTGTATATGTATGCA3060.13860264975653946No Hit
TTTTCTGGGAGAGTTCCTAGGTGAAGAGGCAGACTGCCATCTTTGCTGTTAG3050.13814969992073378No Hit
TAAGTGGGGGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGT3030.13724380024912242No Hit
CCCTTGAGAATTACTTTATCCTTTGCATTTGCATATAGTTTGAATTCATAAT3000.13588495074170537No Hit
ACAAGTGGGAGACTGATGCTCTTCCCTGATGGAGACCCTGAGATCTTCCCCA2990.13543200090589966No Hit
CTTGTTGGGACTGGCTGTCAACACCATGCTGAGTTCCACGAAAGCAGCGAGG2980.134979051070094No Hit
TATACAGGGCTGGTTAAGAGATGTGCCTTTAACTTTGTGAACAAATTTCCAA2980.134979051070094No Hit
CATATAGGGTGTATCATGAAATGATTTGAAATCATTGTAAAGCAGCGAAGTC2960.1340731513984826No Hit
GCAACTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2960.1340731513984826No Hit
TGGAGTGGGAATTTACATTTTTGATAAACAATGTCAGAAATTACTTTTATCT2950.13362020156267693No Hit
GGCTTCGGGATGAGTTATCTGTAAAATGGTTTGGTTGAAATGTTAAGAAAAC2940.13316725172687124No Hit
TTGTTTGGGAGATCTGGGTGAGGTTATGTAGCTGTTTTTGGTACCATTTTAA2940.13316725172687124No Hit
CTTTAGGGTAATGCTTAAGCTAATAATGTAGGTTGGGTTTTCTGTTACTTTC2920.13226135205525988No Hit
TACGATGGGGTCAGGTACTGCATTTACATGTATTAACTAAATCATCAAAAAC2910.1318084022194542No Hit
GTTAAAGGGAGCTATGAATTCTGGGAAGTCAATGTGAAAAACATTGCTGCAT2910.1318084022194542No Hit
TCTCAAGGGCTGCTTTCTAGATGTGTGATATTATTCAAATTAGTTGGCACCC2900.1313554523836485No Hit
TACTTTGGGGATTACAATAAATAGCCTTAAAGAGACTGACTCTAAGCTTCAT2900.1313554523836485No Hit
TATCACGGGACATGAAACACTAATTTTATAGTTGTAGAGTTTCATTGAAATA2880.13044955271203715No Hit
ACCTATGGGATCAAGGAGCCTGCCCCCTCAGCTCATCTCAACTCCTGTATGC2830.1281848035330087No Hit
TAACATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATTTTTTGTCTGCT2830.1281848035330087No Hit
TATGTGGGGATGGCTGCGTTGGATTGACAGAGGCACCAAAAGGCAAATGGTA2820.12773185369720302No Hit
GTCATTGGGAATGAATATGGAAAAGCTTTCAGTCCCACCTCACGACCCACTG2820.12773185369720302No Hit
TGCAATGGGTTTGTGTTGCTATGTACTACAGAAACAAATGAGCTGGATGAAT2780.1259200543539803No Hit
TGTATAGGTCAGTCGGGGATCCTTTGCTGCTTAGCCAAGTGTAAATGTTTAA2770.1254671045181746No Hit
TGCCATGGGAAATACAGAAATATAAGGAAGAAAGAAAAAGATAAAAAGAAAG2760.12501415468236893No Hit
ACTTGCGGGGCGTGAGAGGAAAAAATGTGAAATGTCTCAAATCAAATGCTTC2730.12365530517495188No Hit
ATCGATGGGATCTTGACAGAGCCTGCCCCAGCGCTGACGTGTCAGGAAAACA2720.12320235533914618No Hit
AGCGTAGGGCTTCTACAATATCCCTATTGAGACATCATTGAGTTTCTGTTTT2710.12274940550334051No Hit
CTTTTGGGGCTTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGG2700.12229645566753482No Hit
CTGTACGGGAGCTGGTGCAGTGGTTCTGGCACACGCTGGAAGAGTTCTCCAA2690.12184350583172915No Hit
ATTCGAGGGAGTAGAATTTATGTTTTGGAAATAGATCTCTAATCCAAATAAA2690.12184350583172915No Hit
ATAACTGGGACCGCTTCCCTTGGCTAGGAAAGGGTATTCCCTGACCCCTTGT2650.12003170648850639No Hit
TCATAGGGGAAGCTGGAATGTCATCGCATTTCCAAGACTAACTCAGTGTCCA2650.12003170648850639No Hit
TATAGTGGGAACCTAACAATGCATCTTAAAGAATTAGAAAAGCAAGAGCAAA2650.12003170648850639No Hit
AAAATAGGGAAAGAGATGGGAGGGAAGGCCTGGCCCTTCTGGGGAATAGGAA2620.11867285698108936No Hit
AAGATTGGGGGGCCCAGACTGTTGCCCACTCCATATTCCAAAAGTAGGGGAG2610.11821990714528366No Hit
TTGCTGGGGATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTC2610.11821990714528366No Hit
GATTCTGGGTAACAGGTTCCAATGCATTCACACAATGGAATACTACTCAGCA2600.11776695730947799No Hit
TACAATGGGGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTTTAGAA2590.1173140074736723No Hit
TTACGTGGGATGCCACATCTCCATGAAAATGCATACGTTAATGAACTAATAA2590.1173140074736723No Hit
AAAAATGGGAAATGAGAAAATTATCTTGCTATCTGCAAAGCATTCTACTAAA2580.1168610576378666No Hit
ACTCTGGGAGCTTTGTGTTTTCTCCTTTCTATCTTCTGCCCAAAGTGGGATA2570.11640810780206093No Hit
TTTTTAGGGACATCCTCCCCACCTCATAACGCAGCCACTGAGGAAGAGTGGT2520.1141433586230325No Hit
ATGGCCGGGAAACTGACTTGATCGTGGTGGATAAGCTTTTTGATGTGCTGCT2520.1141433586230325No Hit
GTCACGGGGCGGAGAGAGGAAGGCAAATTCCATCAATGGGTGCTGTTAAGCC2510.1136904087872268No Hit
GCCAGGGCAGGGCACCAAAGACTTTGAATGAGAGGACAGGTGTCATGGGACG2500.11323745895142114No Hit
TAACACAGGGAGATAAAGATCATTATTCTCAGGTTATAGCTGGGAAAACTGG2490.11278450911561544No Hit
GAGGCTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC2480.11233155927980977No Hit
TACCATGGGACTAGAGACATTAGGAAACTAACTGTACAAAAGAACCAAGTTT2480.11233155927980977No Hit
TACGCAGGGAGAGTTGTGCCTGGTTTCATAGTCCAAGGCGGAGATCCTACTG2480.11233155927980977No Hit
TATCAAGGGATGCAGGGCTGTGGACGGGGGAAGGGTTGTGGCGGGTGTTCTG2480.11233155927980977No Hit
TCTGGGGGGGTCGCAGGGAGCTCCGCAGCGACGTGGACCAAGGTGTATGTGG2480.11233155927980977No Hit
TCATACGGGAACCATTTAAGGCTGAGGCATTTTGGTTTTCTGCTGGCTTTTA2480.11233155927980977No Hit
CAGCATGGGATCTCTATAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCA2480.11233155927980977No Hit
CTGGTTGGGGAAGATTTTCTGGGTCTGGAGCTCATTGACAAGCGTAAGGCTA2470.11187860944400407No Hit
CGCCCAGGGGTCTATTTGATCAGTTTCTGTAACCTGAAATATTGGGGAATAG2470.11187860944400407No Hit
ACTGACGGGATAGGATTCAGTATCCAGAGTTTATTTTTAATCTTAATCCTCA2460.1114256596081984No Hit
TGAGGTGGGCTCTGGACTGGAGAATAATGAAGAAGTTCACTGACTGTGCACT2460.1114256596081984No Hit
GTTAATGGGGGCCGGGCTTGTCTCGAAATCCTGACCTCCTGAAGGTCAGGAC2460.1114256596081984No Hit
CAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2460.1114256596081984No Hit
ATTGCCGGGAGTGGAGGTCCAGTACTTCCAGCATCTGACTTTTTACAGGCCA2460.1114256596081984No Hit
TTGCAAGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGTT2450.11097270977239271No Hit
GCATATGGGTATATTCTAAATTTTCAAACTGTGATGTTTATTTTATACTTGT2450.11097270977239271No Hit
AGAACTGGGGACAACACTTATTTTCAACACCTGCCATTGCTGTTGCTTTTTC2440.11051975993658701No Hit
TGGAATGGGGTTTTCACGAGAGTTGTAATGAATTTGTAAATCACACTGAAGA2430.11006681010078134No Hit
GTTGTGGGGGTGCAAGCTGCTTATCTAGAATACTGCAGTCCTGAGGAATTTC2430.11006681010078134No Hit
GAATAGGGGAAGTAATATCTAACTCAAAATCCAAGGTAGAACATAGATAAAA2420.10961386026497565No Hit
TGAGCAGGGACCAAAGCAATTCCCCATGCTAAAACCTTAAATTAACACAGAA2420.10961386026497565No Hit
GCTATAGGGAGGGGCTTTGGACAAGTCACATCCCCTCCTTCTGAGTTAATGA2420.10961386026497565No Hit
TCTCAAGGGAGAAGGAAAATTGGAAAATACACAGATATGTAGAAATTAAATA2420.10961386026497565No Hit
ATAAGTAGGGGTAGAGAAGCCATGGAGAACAGACTTGAAAAGTTTAGGAAAT2420.10961386026497565No Hit
TGCGTTGGGAAACAAAATTTTTAAGAGATTGAGGTATAATTGTCAAACAGCA2410.10916091042916998No Hit
TTATGGGGGCTCTTCCGATCTTCTGCAGGGGACACAGGCAAGGAATCACAAC2410.10916091042916998No Hit
TTATCTGGGCTGGAATAGGAGCTTTAACTGGAAATTGCTGACTAATTTTATG2400.10870796059336428No Hit
ACGTTTGGGGGAACAGCAGAGCCTGCTCCTTGCGTACCACAGGGATTAATCC2400.10870796059336428No Hit
CCATAAGGATGTTTCATATATGCCTGTTAAGTCCGTTTGGTCTAAAGTGTAA2390.10825501075755861No Hit
GTTCATGGGATTTATTCATATCATGCCTTATGGAAATTTCTTTTTCTGTATT2380.10780206092175292No Hit
CAGCCTGGCCAACAAGGTGAAACCCTGTCTCTACTGATAATACAAAAATTAG2370.10734911108594725No Hit
CAGTGCGGGCTATCCATGGAGGTGTGACTGGCACTAGCCTAGCCCTCCAAAA2370.10734911108594725No Hit
TTCACTGGGAGGACGAGAGCCCACCTGAATCCTGTGAGCTGGGTAAGTGAGG2370.10734911108594725No Hit
GGTATAGGGAGATTTAATTGCCCAGTGAATGGCACTTACGTTTTCATTTTTC2360.10689616125014155No Hit
AGCAATGGGCAAAACCACTTAGAAAACTAGAATAGAGAACCATAACATGTAC2360.10689616125014155No Hit
GGTTTGGGAGCTTCATTTTCTACTAGCACCTATCATAATGGTCTTAGTCATT2360.10689616125014155No Hit
GTTGCAGGGATGAAAGAAAAGGCACTTCTTGGACAAGCACTGAAATATAATG2360.10689616125014155No Hit
GTGAAGGGGAATCCAAATTCTACAATGGATAAGTATGATTTTAATAAATAGA2360.10689616125014155No Hit
CGCATGGGGGCATGGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10689616125014155No Hit
ACTACCGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTGG2350.10644321141433585No Hit
CGCTATGGGACTGTTTTCCAGAGTGGCTGCATTATATTGCATTCCCACCAAC2350.10644321141433585No Hit
CTAACTGGGGGCTCATGCCTATAGTCATAGCACTTTGGGAGGCTGAGGTGGG2350.10644321141433585No Hit
GCGAATGGGCATGAGCCACCATGCCCAGTTGCATGTTTTAATGGAGAGAGAT2350.10644321141433585No Hit
GTGCATGGGTAAGTATTTAAATGTATTAAATTTCATTAAGATTAAACATGTA2340.10599026157853018No Hit
GCACAGGGGCAGTCAGGGACATACTTTGTGCAATATTTCTGTGATTGTGCCT2340.10599026157853018No Hit
CTCAGAAGGGAAAAGGACAAATTTTTATTCAACCCAATTAAATTCAACAAGT2330.10553731174272449No Hit
TGAGGGGATGTGCTGGGTGCCATAAGATGCATTCCAGCCCTGCAAGAACTCA2330.10553731174272449No Hit
TGATGTGGGGGTTATTGAGCACTTAAATGTGGCTAGTTTGATGGAGGAGCTG2330.10553731174272449No Hit
CCTTTGGGGGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATTAAGCAG2330.10553731174272449No Hit
CAGGTATTAACTACTACTTGCTAGTAGTGGATAGGAAGTGGTAGCAGCACTC2320.10508436190691882No Hit
TTCTTGCGGGATTTGTTATTTGGTACAATCTCAGTGGAACCAATTGTGCCCT2310.10463141207111312No Hit
TTGCCTGGGGAATGGCCATAATCAAAAAATCAAAAAATAATAGATGTTGGCA2310.10463141207111312No Hit
AGAAGCGGGCTAGAGCAGTGGGAAATGCATGCACCACTCAACATTCTGACAT2310.10463141207111312No Hit
ATTAACGGGATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTT2310.10463141207111312No Hit
TCTTGTGGGGAACTGTTCGATAAAACAAGAAAAATAGAAGCACGAGTGTCTG2310.10463141207111312No Hit
ACCAGCGGGCAGAGGCTGGGCCTGAACAGGGAGGTGGTCGCTCAGGCCTGGT2310.10463141207111312No Hit
AAAGAGGGACAGAAACAATAGCTGATACATTGCAGTAAAGTGTTCATCTTAG2300.10417846223530745No Hit
AATTTAGGGCCGATCCGTGAAGGGGGACTCCAATTCCTGCAGTTCTTTTCAC2300.10417846223530745No Hit
TACTCCGGGGGTTCATTTCTGACATGACTGTCACCTAATATGAGGTTGATTC2300.10417846223530745No Hit
ATGCACGGGACCTTTTGTTTGCCCGACACAATGTTTTGAAAAACTGAATCAA2300.10417846223530745No Hit
CAATATGGGATTTTAGATGCCATTTAGAACTTTTGTGATTCATTGGACGAGG2290.10372551239950176No Hit
GTCGATGGGATAGAAAGTATACTCTAAAGGGAATTTGCCGAAGACCTTTTAC2280.10327256256369606No Hit
ATGCCTGGGATTTTATGCATTGTCACAAAATTCCATAATGTAAGAAATTTAC2280.10327256256369606No Hit
AAGCAGGGGAACATAGTCTTAAAATTACAGTTTGCTTGAATGTCTCAGAATA2280.10327256256369606No Hit
ATCACGCAGGGACAAATGAAGAAACTAAACCTCAGGGATGTTGGTAACACAG2270.10281961272789039No Hit
CGCAAAGGGAAGGGAAAGAACCTGAGCTCTTCCTAAGTAGATCCAGGCCTGT2250.10191371305627903No Hit
CGGCCGGGGTGTTCAGAGCTTGTCTCCACCTCACGGCTCTGTGCTCTTCTGT2250.10191371305627903No Hit
CTCTTAGGGGACGCTCTTCCGATCTCAGCAGGGGATGTGGACATTGCCATCC2250.10191371305627903No Hit
GTGGCCGGAGAGGTATGGATTAACTTAGGTTTTCGGACGTTAAGTTTTTCTG2240.10146076322047333No Hit
TTGCCAGGGTGGTCTGTCCTTTTAACTGCTTCTAAGTGAACGAAGTGAGGAG2220.10055486354886196No Hit
TTGCGCGGGGGTATAATAAATGACCAGATGAATGAATGAGTGAAGCAAGAGA2220.10055486354886196No Hit
TATGGAGGGAAATCTAGAGTCTTGATTAGGTTTAAATTCATTTGTTTTTCTT2220.10055486354886196No Hit
AACGGTGGGGCAGTTGGACACATATATTTTTTCTTGGAAGATGTATTTCCCA2210.1001019137130563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)