FastQCFastQC Report
Sat 31 Dec 2022
SRR5846118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219096
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT9450.43131777850805125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC8780.4007375762222953No Hit
CTCTATGGGACTTCCTTAGGAGTCATTCATTTGTATATTTGACACTTTTGCT4180.1907839485887465No Hit
TGTACAGGGTGTGGGGAATGTGGGAAATCCTTTAGCCATAGCTCCAACCTTA3650.16659363931792456No Hit
TATACAGGGCTGGTTAAGAGATGTGCCTTTAACTTTGTGAACAAATTTCCAA3650.16659363931792456No Hit
GCAACGGGGCGATCTAATTTGGGGGGGATTCACACAATACTTGACAATCACA3510.16020374630299047No Hit
AAACACGGGAGAAACAGAAAAAAGCAATCCTAAAATTCATATGGAAACACAA3450.1574652207251616No Hit
GCAACAGGGCTGGTTCTTAAAGCCAATCAATAAACAGATTTTGAAAAAGCTT3390.15472669514733267No Hit
TAGACTGGGTCTTGATGTGAATTCCACTGTTACCTGACCATGTATATTCAGA3340.1524445904991419No Hit
TGAGTCGGGAGGACCTCCTGGAACTGACTTCTTCTGTCAGGAGGACTGGTTT3330.15198816956950378No Hit
TGCAATGGGTTTGTGTTGCTATGTACTACAGAAACAAATGAGCTGGATGAAT3280.14970606492131303No Hit
GGCTTCGGGATGAGTTATCTGTAAAATGGTTTGGTTGAAATGTTAAGAAAAC3270.1492496439916749No Hit
TTTTCTGGGAGAGTTCCTAGGTGAAGAGGCAGACTGCCATCTTTGCTGTTAG3260.14879322306203674No Hit
CTTGTTGGGACTGGCTGTCAACACCATGCTGAGTTCCACGAAAGCAGCGAGG3230.14742396027312227No Hit
ACAAGTGGGAGACTGATGCTCTTCCCTGATGGAGACCCTGAGATCTTCCCCA3210.14651111841384598No Hit
GAAAACGGGCGTGGGCGGACGACTGGAAGGCCATTCTGGACAGCAAGCGGCC3190.1455982765545697No Hit
TTTCTGGGTGACGTGTGGGTCTTTTGTTAAAGAGAGCAGTGTTAGAGTCAAT3140.14331617190637894No Hit
TTGTTTGGGAGATCTGGGTGAGGTTATGTAGCTGTTTTTGGTACCATTTTAA3140.14331617190637894No Hit
CGAAACGGGACAATGGAATATTATTCAGCTTTAAAAAAGAAGGAAATCTTGT3120.14240333004710265No Hit
TAACTAGGGCTGCTGTTTCTTTTATTTTCTATTGCATTGATTTTTATTCTAA3110.1419469091174645No Hit
CTTTAGGGTAATGCTTAAGCTAATAATGTAGGTTGGGTTTTCTGTTACTTTC3080.14057764632855002No Hit
CTTTTGGGGCTTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGG3080.14057764632855002No Hit
GCCAGGGCAGGGCACCAAAGACTTTGAATGAGAGGACAGGTGTCATGGGACG3060.13966480446927373No Hit
AACAATGGGTCGAGATCACAAGTTGCATGTTGACTTTAGGAACGTGAAGCTC3050.1392083835396356No Hit
GGTATGGGGCGACCTAAAGCAGGGACGGAGGTCTTGTGTATCTGTGGCAACA3040.13875196260999745No Hit
TGCGTTGGGAAACAAAATTTTTAAGAGATTGAGGTATAATTGTCAAACAGCA2950.1346441742432541No Hit
GCGAATGGGCATGAGCCACCATGCCCAGTTGCATGTTTTAATGGAGAGAGAT2940.13418775331361596No Hit
ATTAGCGGGAAATATAAAAAGTAACATTTTATTGAAATGTATTTCTTAATTA2920.13327491145433965No Hit
GCAACTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2920.13327491145433965No Hit
AGCGTAGGGCTTCTACAATATCCCTATTGAGACATCATTGAGTTTCTGTTTT2920.13327491145433965No Hit
TGGAGTGGGAATTTACATTTTTGATAAACAATGTCAGAAATTACTTTTATCT2900.13236206959506336No Hit
GCAAACGGCTTCCGATCTGAAAGTGGGCAGTCCGTGGAGAGAACAAAACCCA2890.1319056486654252No Hit
TGAGGGGATGTGCTGGGTGCCATAAGATGCATTCCAGCCCTGCAAGAACTCA2890.1319056486654252No Hit
CCCTTGAGAATTACTTTATCCTTTGCATTTGCATATAGTTTGAATTCATAAT2890.1319056486654252No Hit
GTCATTGGGAATGAATATGGAAAAGCTTTCAGTCCCACCTCACGACCCACTG2890.1319056486654252No Hit
GTTCATGGGATTTATTCATATCATGCCTTATGGAAATTTCTTTTTCTGTATT2880.13144922773578707No Hit
TCATAGGGGAAGCTGGAATGTCATCGCATTTCCAAGACTAACTCAGTGTCCA2880.13144922773578707No Hit
TACGATGGGGTCAGGTACTGCATTTACATGTATTAACTAAATCATCAAAAAC2860.13053638587651076No Hit
ACCTATGGGATCAAGGAGCCTGCCCCCTCAGCTCATCTCAACTCCTGTATGC2850.1300799649468726No Hit
TACTTTGGGGATTACAATAAATAGCCTTAAAGAGACTGACTCTAAGCTTCAT2800.12779786029868187No Hit
ATCGATGGGATCTTGACAGAGCCTGCCCCAGCGCTGACGTGTCAGGAAAACA2790.1273414393690437No Hit
AAAAATGGGAAATGAGAAAATTATCTTGCTATCTGCAAAGCATTCTACTAAA2790.1273414393690437No Hit
ATGGCCGGGAAACTGACTTGATCGTGGTGGATAAGCTTTTTGATGTGCTGCT2760.12597217658012924No Hit
ATAACTGGGACCGCTTCCCTTGGCTAGGAAAGGGTATTCCCTGACCCCTTGT2760.12597217658012924No Hit
ATGTGCGGGACTTCAAATGCCAGCTGAAATCCAGACGGTGATTGGCCTCAAA2750.1255157556504911No Hit
ACCTCAGGGAACTGGTTTTTATTACATTAAGTTATACCTGTATATGTATGCA2730.12460291379121481No Hit
TACGCAGGGAGAGTTGTGCCTGGTTTCATAGTCCAAGGCGGAGATCCTACTG2720.12414649286157665No Hit
ACTTGCGGGGCGTGAGAGGAAAAAATGTGAAATGTCTCAAATCAAATGCTTC2720.12414649286157665No Hit
TAACACAGGGAGATAAAGATCATTATTCTCAGGTTATAGCTGGGAAAACTGG2720.12414649286157665No Hit
ACACGTGGGAGAAAGGGAAATAAAAAGTAAGTGAAAGCGAGCGCCACCAGAA2710.12369007193193851No Hit
TTCTTGCGGGATTTGTTATTTGGTACAATCTCAGTGGAACCAATTGTGCCCT2690.12277723007266222No Hit
TTTTTAGGGACATCCTCCCCACCTCATAACGCAGCCACTGAGGAAGAGTGGT2680.12232080914302407No Hit
AATTTAGGGCCGATCCGTGAAGGGGGACTCCAATTCCTGCAGTTCTTTTCAC2640.12049512542447145No Hit
CGCCCAGGGGTCTATTTGATCAGTTTCTGTAACCTGAAATATTGGGGAATAG2640.12049512542447145No Hit
TATCACGGGACATGAAACACTAATTTTATAGTTGTAGAGTTTCATTGAAATA2630.12003870449483331No Hit
ACGTTTGGGGGAACAGCAGAGCCTGCTCCTTGCGTACCACAGGGATTAATCC2630.12003870449483331No Hit
TTGCTGGGGATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTC2630.12003870449483331No Hit
TATGTGGGGATGGCTGCGTTGGATTGACAGAGGCACCAAAAGGCAAATGGTA2620.11958228356519518No Hit
GTTAAAGGGAGCTATGAATTCTGGGAAGTCAATGTGAAAAACATTGCTGCAT2620.11958228356519518No Hit
CTGTACGGGAGCTGGTGCAGTGGTTCTGGCACACGCTGGAAGAGTTCTCCAA2610.11912586263555702No Hit
GGTTTGGGAGCTTCATTTTCTACTAGCACCTATCATAATGGTCTTAGTCATT2610.11912586263555702No Hit
CACAGGGGAAGCTGACAGGGAAGGGGCAGAGGAGGATCCTCTATTGTTTCCT2610.11912586263555702No Hit
GTTAATGGGGGCCGGGCTTGTCTCGAAATCCTGACCTCCTGAAGGTCAGGAC2590.11821302077628072No Hit
AACGGTGGGGCAGTTGGACACATATATTTTTTCTTGGAAGATGTATTTCCCA2580.11775659984664257No Hit
GCTATAGGGAGGGGCTTTGGACAAGTCACATCCCCTCCTTCTGAGTTAATGA2580.11775659984664257No Hit
TTGCAAGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGTT2570.11730017891700441No Hit
ATTAACGGGATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTT2570.11730017891700441No Hit
TATAGTGGGAACCTAACAATGCATCTTAAAGAATTAGAAAAGCAAGAGCAAA2570.11730017891700441No Hit
GAATAGGGGAAGTAATATCTAACTCAAAATCCAAGGTAGAACATAGATAAAA2560.11684375798736628No Hit
ATGCACGGGACCTTTTGTTTGCCCGACACAATGTTTTGAAAAACTGAATCAA2550.11638733705772812No Hit
TCTGGGGGGGTCGCAGGGAGCTCCGCAGCGACGTGGACCAAGGTGTATGTGG2540.11593091612808998No Hit
TGTCAGGGGACGGTCTTCATATAATTAATTGAAAACACCCCTGAGCAAAGTG2540.11593091612808998No Hit
ACTACCGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTGG2530.11547449519845182No Hit
TTATCTGGGCTGGAATAGGAGCTTTAACTGGAAATTGCTGACTAATTTTATG2530.11547449519845182No Hit
ATAGTGGGGACCAAACGCTACTGGTTCTTTTACATATGCACTACCTAATATA2530.11547449519845182No Hit
TACTCCGGGGGTTCATTTCTGACATGACTGTCACCTAATATGAGGTTGATTC2520.11501807426881366No Hit
TCTCAAGGGCTGCTTTCTAGATGTGTGATATTATTCAAATTAGTTGGCACCC2500.11410523240953736No Hit
CAGCATGGGATCTCTATAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCA2500.11410523240953736No Hit
TAACATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATTTTTTGTCTGCT2480.11319239055026108No Hit
GCACAGGGGCAGTCAGGGACATACTTTGTGCAATATTTCTGTGATTGTGCCT2470.11273596962062292No Hit
GGCATAGGGGTTCTAAAAATTTTATCGCTGTTAAGGTATTAATCATTCAGTA2470.11273596962062292No Hit
GTGCATGGGTAAGTATTTAAATGTATTAAATTTCATTAAGATTAAACATGTA2460.11227954869098478No Hit
TCTCAAGGGAGAAGGAAAATTGGAAAATACACAGATATGTAGAAATTAAATA2460.11227954869098478No Hit
AGTGCCGGGATACCAAGTCCTTCGTGAAGCACTCCATGGAGCATGTGTCAAT2460.11227954869098478No Hit
CACTGGGGGCATATTCTAGGCAAAGGAACTTAAAACTTCTTGGAGGGAAAAT2450.11182312776134662No Hit
GTTGTGGGGGTGCAAGCTGCTTATCTAGAATACTGCAGTCCTGAGGAATTTC2450.11182312776134662No Hit
AAACACGGGCAGTAGTCATGATTTAAAGTTATGAAACCGCCATTGCAAAATT2440.11136670683170846No Hit
TGGAATGGGGTTTTCACGAGAGTTGTAATGAATTTGTAAATCACACTGAAGA2440.11136670683170846No Hit
TACAATGGGGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTTTAGAA2440.11136670683170846No Hit
AGAACTGGGGACAACACTTATTTTCAACACCTGCCATTGCTGTTGCTTTTTC2430.11091028590207033No Hit
ACTGACGGGATAGGATTCAGTATCCAGAGTTTATTTTTAATCTTAATCCTCA2430.11091028590207033No Hit
TTACGTGGGATGCCACATCTCCATGAAAATGCATACGTTAATGAACTAATAA2430.11091028590207033No Hit
TAAGTGGGGGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGT2420.11045386497243219No Hit
ATTCGAGGGAGTAGAATTTATGTTTTGGAAATAGATCTCTAATCCAAATAAA2420.11045386497243219No Hit
GATTCTGGGTAACAGGTTCCAATGCATTCACACAATGGAATACTACTCAGCA2400.10954102311315587No Hit
GTCTAGGGGAGCTCACTGCAGCTTCAACTTCCTGGTCTCAAGTGTTCCTCCC2400.10954102311315587No Hit
TACAATGGGGAAGTGAAGTCATTATTGAGTCATGAATGGAGTGTAAGTGTGG2390.10908460218351773No Hit
AGTAGGGGAGTAACTTGAAATAATGAAAAGGACGTTTATACCAAATTAAGGA2390.10908460218351773No Hit
TGCCATGGGAAATACAGAAATATAAGGAAGAAAGAAAAAGATAAAAAGAAAG2390.10908460218351773No Hit
GACAGGGATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTT2390.10908460218351773No Hit
ACTCTGGGAGCTTTGTGTTTTCTCCTTTCTATCTTCTGCCCAAAGTGGGATA2390.10908460218351773No Hit
CAGCCTGGCCAACAAGGTGAAACCCTGTCTCTACTGATAATACAAAAATTAG2380.10862818125387957No Hit
ATGCCTGGGATTTTATGCATTGTCACAAAATTCCATAATGTAAGAAATTTAC2380.10862818125387957No Hit
ATATGTGGGGTGATATTTTTCTGTGTGTTTTATTTTGTTGTTGTTTTAAGAT2380.10862818125387957No Hit
ACCAGCGGGCAGAGGCTGGGCCTGAACAGGGAGGTGGTCGCTCAGGCCTGGT2380.10862818125387957No Hit
AAGCAGGGGAACATAGTCTTAAAATTACAGTTTGCTTGAATGTCTCAGAATA2380.10862818125387957No Hit
GAGGCTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC2370.10817176032424142No Hit
CATATAGGGTGTATCATGAAATGATTTGAAATCATTGTAAAGCAGCGAAGTC2360.10771533939460329No Hit
GTCGATGGGATAGAAAGTATACTCTAAAGGGAATTTGCCGAAGACCTTTTAC2360.10771533939460329No Hit
TGTATAGGTCAGTCGGGGATCCTTTGCTGCTTAGCCAAGTGTAAATGTTTAA2350.10725891846496513No Hit
TTCACTGGGAGGACGAGAGCCCACCTGAATCCTGTGAGCTGGGTAAGTGAGG2350.10725891846496513No Hit
AAAATGGGATGTCCACTTATCAACAAGAAATTGTAAGACATACAAAGAAACT2350.10725891846496513No Hit
GTCACGGGGCGGAGAGAGGAAGGCAAATTCCATCAATGGGTGCTGTTAAGCC2330.10634607660568883No Hit
TCATACGGGAACCATTTAAGGCTGAGGCATTTTGGTTTTCTGCTGGCTTTTA2330.10634607660568883No Hit
TGATGTGGGGGTTATTGAGCACTTAAATGTGGCTAGTTTGATGGAGGAGCTG2320.10588965567605067No Hit
CCTTTGGGGGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATTAAGCAG2320.10588965567605067No Hit
CAGGTATTAACTACTACTTGCTAGTAGTGGATAGGAAGTGGTAGCAGCACTC2320.10588965567605067No Hit
AAAAGTGGGGCCTGTCACCCCAAATGCAGCAATGGCCTGGTGATTCCCACAC2310.10543323474641253No Hit
ATATCAGGGACCTCAAGGAACTAGAGAAACAAGAACAAACCAAACTCAAACC2310.10543323474641253No Hit
TGAGCAGGGACCAAAGCAATTCCCCATGCTAAAACCTTAAATTAACACAGAA2300.10497681381677439No Hit
ATAAGTAGGGGTAGAGAAGCCATGGAGAACAGACTTGAAAAGTTTAGGAAAT2300.10497681381677439No Hit
CGCTATGGGACTGTTTTCCAGAGTGGCTGCATTATATTGCATTCCCACCAAC2290.10452039288713624No Hit
AAAGAGGGACAGAAACAATAGCTGATACATTGCAGTAAAGTGTTCATCTTAG2280.10406397195749809No Hit
GTAGTTGGGGTGCGGAACCTCCGAGAACGACGACCAGCTGCTGTTTTGTGAT2280.10406397195749809No Hit
CTGGTTGGGGAAGATTTTCTGGGTCTGGAGCTCATTGACAAGCGTAAGGCTA2280.10406397195749809No Hit
CGCATGGGGGCATGGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.10406397195749809No Hit
GCATATGGGTATATTCTAAATTTTCAAACTGTGATGTTTATTTTATACTTGT2280.10406397195749809No Hit
AAAATAGGGAAAGAGATGGGAGGGAAGGCCTGGCCCTTCTGGGGAATAGGAA2280.10406397195749809No Hit
ACATTAGGGATGACGGTTGTAACTACTAAACCAAATTTAGTTTTCACAGCAT2270.10360755102785993No Hit
TGAGGTGGGCTCTGGACTGGAGAATAATGAAGAAGTTCACTGACTGTGCACT2270.10360755102785993No Hit
AAGATTGGGGGGCCCAGACTGTTGCCCACTCCATATTCCAAAAGTAGGGGAG2270.10360755102785993No Hit
TATGGAGGGAAATCTAGAGTCTTGATTAGGTTTAAATTCATTTGTTTTTCTT2270.10360755102785993No Hit
CGCAAAGGGAAGGGAAAGAACCTGAGCTCTTCCTAAGTAGATCCAGGCCTGT2260.10315113009822179No Hit
ATACAAGGGCGATCTGTACAGGGGCTCTTTCCTTGCCTAACGCAGCCATGGC2260.10315113009822179No Hit
AACCAGGGGAGGGAGAGGTTCTGCCACTGAAGGAATGTTTGTGATGGTTGTA2260.10315113009822179No Hit
CCATAAGGATGTTTCATATATGCCTGTTAAGTCCGTTTGGTCTAAAGTGTAA2260.10315113009822179No Hit
CTAACTGGGGGCTCATGCCTATAGTCATAGCACTTTGGGAGGCTGAGGTGGG2260.10315113009822179No Hit
AGCAATGGGCAAAACCACTTAGAAAACTAGAATAGAGAACCATAACATGTAC2250.10269470916858363No Hit
ATTGCCGGGAGTGGAGGTCCAGTACTTCCAGCATCTGACTTTTTACAGGCCA2250.10269470916858363No Hit
CTAGCAGGGGAGCTTACCAGTGATCTTCTGATCTTCAAGAAGACTAAGTTTG2250.10269470916858363No Hit
AGAAGCGGGCTAGAGCAGTGGGAAATGCATGCACCACTCAACATTCTGACAT2240.10223828823894547No Hit
GCCAAGGGAATCCAGACAATGACTGTGCATTTTGAGAGGCTAGTAGGTGAGA2240.10223828823894547No Hit
GGGTACGGGATTACTTACTGTTAAAACTGTACCTTTTGCGATTTCACAGTTG2240.10223828823894547No Hit
AGTTAAGGGGATCTGTGACATATGGGTTATATTATTGTGTCTTTGTCAAACA2230.10178186730930734No Hit
GTGCTTGGGAGACAGAGGTCTATATAGGTCCATCATGGGAATCAGAGGTTGA2230.10178186730930734No Hit
CAATATGGGATTTTAGATGCCATTTAGAACTTTTGTGATTCATTGGACGAGG2220.1013254463796692No Hit
GCACTGTGGGAGAGAAAGTTTGCCATGATCATGCTTTCCTCATTACACATCA2220.1013254463796692No Hit
GTGGCCGGAGAGGTATGGATTAACTTAGGTTTTCGGACGTTAAGTTTTTCTG2210.10086902545003104No Hit
ATATTAGGGACAAAGCCACATTTCTATTTGTGGACGTCACATAAGGCTTAAC2210.10086902545003104No Hit
GTTCTGGGGATGTGAAGGCTCTTGATGTTTGGCTTATTGCTTGCCTTCTCTT2210.10086902545003104No Hit
TTCCAAGGGGAATCCAAACCTCATGTATCAATGGGGAATTTTCAAATATAAA2210.10086902545003104No Hit
AGTGGGGAAGGGAGGTTGAACCTGGGTCTCTGTCTCATGTGCCATAATAGTA2210.10086902545003104No Hit
TTGCGCGGGGGTATAATAAATGACCAGATGAATGAATGAGTGAAGCAAGAGA2210.10086902545003104No Hit
CTCAGAAGGGAAAAGGACAAATTTTTATTCAACCCAATTAAATTCAACAAGT2200.10041260452039288No Hit
GAACTGGGTCATGGTGGAAGGTGAAGGGGGAGCAGGCAGATCACATGGCGAG2200.10041260452039288No Hit
AACGTGGGGCTACTGTTACAGTTACACTACTAAATGCTGATAACTATCATCA2200.10041260452039288No Hit
GTGTCAGGGAAGGCTGAAAGCCTCAAGTGAAGGATCTTGCAGCTTGTAAAAG2200.10041260452039288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)