FastQCFastQC Report
Sat 31 Dec 2022
SRR5846117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218575
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC8850.40489534484730644No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT8720.39894772961226127No Hit
CTCTATGGGACTTCCTTAGGAGTCATTCATTTGTATATTTGACACTTTTGCT3760.17202333295207595No Hit
GCAACAGGGCTGGTTCTTAAAGCCAATCAATAAACAGATTTTGAAAAAGCTT3660.1674482443097335No Hit
TGTACAGGGTGTGGGGAATGTGGGAAATCCTTTAGCCATAGCTCCAACCTTA3600.16470319112432802No Hit
TAACTAGGGCTGCTGTTTCTTTTATTTTCTATTGCATTGATTTTTATTCTAA3490.15967059361775135No Hit
GCAACGGGGCGATCTAATTTGGGGGGGATTCACACAATACTTGACAATCACA3480.1592130847535171No Hit
AAACACGGGAGAAACAGAAAAAAGCAATCCTAAAATTCATATGGAAACACAA3420.15646803156811165No Hit
CGAAACGGGACAATGGAATATTATTCAGCTTTAAAAAAGAAGGAAATCTTGT3260.14914788974036372No Hit
ATGTGCGGGACTTCAAATGCCAGCTGAAATCCAGACGGTGATTGGCCTCAAA3240.14823287201189522No Hit
GAAAACGGGCGTGGGCGGACGACTGGAAGGCCATTCTGGACAGCAAGCGGCC3220.14731785428342675No Hit
TATGTGGGGATGGCTGCGTTGGATTGACAGAGGCACCAAAAGGCAAATGGTA3180.14548781882648976No Hit
TATAGTGGGAACCTAACAATGCATCTTAAAGAATTAGAAAAGCAAGAGCAAA3110.14228525677685006No Hit
TTGTTTGGGAGATCTGGGTGAGGTTATGTAGCTGTTTTTGGTACCATTTTAA3110.14228525677685006No Hit
TATACAGGGCTGGTTAAGAGATGTGCCTTTAACTTTGTGAACAAATTTCCAA3090.14137023904838156No Hit
ATTAGCGGGAAATATAAAAAGTAACATTTTATTGAAATGTATTTCTTAATTA3050.1395402035914446No Hit
GGCTTCGGGATGAGTTATCTGTAAAATGGTTTGGTTGAAATGTTAAGAAAAC3000.13725265927027336No Hit
TGCGTTGGGAAACAAAATTTTTAAGAGATTGAGGTATAATTGTCAAACAGCA2970.13588013267757063No Hit
TAGACTGGGTCTTGATGTGAATTCCACTGTTACCTGACCATGTATATTCAGA2970.13588013267757063No Hit
TACGATGGGGTCAGGTACTGCATTTACATGTATTAACTAAATCATCAAAAAC2950.13496511494910216No Hit
TTTTCTGGGAGAGTTCCTAGGTGAAGAGGCAGACTGCCATCTTTGCTGTTAG2930.13405009722063366No Hit
TCTCAAGGGCTGCTTTCTAGATGTGTGATATTATTCAAATTAGTTGGCACCC2920.1335925883563994No Hit
ACTTGCGGGGCGTGAGAGGAAAAAATGTGAAATGTCTCAAATCAAATGCTTC2920.1335925883563994No Hit
AAAAATGGGAAATGAGAAAATTATCTTGCTATCTGCAAAGCATTCTACTAAA2920.1335925883563994No Hit
AACAATGGGTCGAGATCACAAGTTGCATGTTGACTTTAGGAACGTGAAGCTC2890.13222006176369666No Hit
TGAGTCGGGAGGACCTCCTGGAACTGACTTCTTCTGTCAGGAGGACTGGTTT2880.13176255289946243No Hit
CCCTTGAGAATTACTTTATCCTTTGCATTTGCATATAGTTTGAATTCATAAT2880.13176255289946243No Hit
GGTATGGGGCGACCTAAAGCAGGGACGGAGGTCTTGTGTATCTGTGGCAACA2870.1313050440352282No Hit
GCAACTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2870.1313050440352282No Hit
GCAAACGGCTTCCGATCTGAAAGTGGGCAGTCCGTGGAGAGAACAAAACCCA2830.12947500857829122No Hit
CTTGTTGGGACTGGCTGTCAACACCATGCTGAGTTCCACGAAAGCAGCGAGG2810.1285599908498227No Hit
TTATCTGGGCTGGAATAGGAGCTTTAACTGGAAATTGCTGACTAATTTTATG2800.12810248198558846No Hit
AGCGTAGGGCTTCTACAATATCCCTATTGAGACATCATTGAGTTTCTGTTTT2790.12764497312135423No Hit
TACGCAGGGAGAGTTGTGCCTGGTTTCATAGTCCAAGGCGGAGATCCTACTG2770.12672995539288573No Hit
CTTTTGGGGCTTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGG2770.12672995539288573No Hit
ACCTCAGGGAACTGGTTTTTATTACATTAAGTTATACCTGTATATGTATGCA2760.1262724465286515No Hit
TATCACGGGACATGAAACACTAATTTTATAGTTGTAGAGTTTCATTGAAATA2730.12489991993594876No Hit
ACAAGTGGGAGACTGATGCTCTTCCCTGATGGAGACCCTGAGATCTTCCCCA2730.12489991993594876No Hit
TACAATGGGGAACTGCTTTCGTCCATGTTTCTCACATTGCTGTATTTTAGAA2720.12444241107171451No Hit
TACTCCGGGGGTTCATTTCTGACATGACTGTCACCTAATATGAGGTTGATTC2710.12398490220748026No Hit
CTTTAGGGTAATGCTTAAGCTAATAATGTAGGTTGGGTTTTCTGTTACTTTC2690.12306988447901178No Hit
ATCGATGGGATCTTGACAGAGCCTGCCCCAGCGCTGACGTGTCAGGAAAACA2690.12306988447901178No Hit
TTTTTAGGGACATCCTCCCCACCTCATAACGCAGCCACTGAGGAAGAGTGGT2690.12306988447901178No Hit
TTGCGCGGGGGTATAATAAATGACCAGATGAATGAATGAGTGAAGCAAGAGA2690.12306988447901178No Hit
CATATAGGGTGTATCATGAAATGATTTGAAATCATTGTAAAGCAGCGAAGTC2670.1221548667505433No Hit
TTTCTGGGTGACGTGTGGGTCTTTTGTTAAAGAGAGCAGTGTTAGAGTCAAT2660.12169735788630903No Hit
TGCCATGGGAAATACAGAAATATAAGGAAGAAAGAAAAAGATAAAAAGAAAG2660.12169735788630903No Hit
CGCATGGGGGCATGGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.12123984902207481No Hit
CCATAAGGATGTTTCATATATGCCTGTTAAGTCCGTTTGGTCTAAAGTGTAA2650.12123984902207481No Hit
TTACGTGGGATGCCACATCTCCATGAAAATGCATACGTTAATGAACTAATAA2640.12078234015784055No Hit
CTAACTGGGGGCTCATGCCTATAGTCATAGCACTTTGGGAGGCTGAGGTGGG2640.12078234015784055No Hit
TACTTTGGGGATTACAATAAATAGCCTTAAAGAGACTGACTCTAAGCTTCAT2630.12032483129360633No Hit
TAACATGGGACTGCACTTACGCTATCCAGTTGCAGGTAATTTTTTGTCTGCT2630.12032483129360633No Hit
TGAGGGGATGTGCTGGGTGCCATAAGATGCATTCCAGCCCTGCAAGAACTCA2620.11986732242937206No Hit
TGAGGTGGGCTCTGGACTGGAGAATAATGAAGAAGTTCACTGACTGTGCACT2620.11986732242937206No Hit
CAGCATGGGATCTCTATAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCA2620.11986732242937206No Hit
GTCTAGGGGAGCTCACTGCAGCTTCAACTTCCTGGTCTCAAGTGTTCCTCCC2610.11940981356513782No Hit
TAACACAGGGAGATAAAGATCATTATTCTCAGGTTATAGCTGGGAAAACTGG2600.11895230470090358No Hit
AAAATAGGGAAAGAGATGGGAGGGAAGGCCTGGCCCTTCTGGGGAATAGGAA2600.11895230470090358No Hit
ATGCCTGGGATTTTATGCATTGTCACAAAATTCCATAATGTAAGAAATTTAC2590.11849479583666933No Hit
TCATACGGGAACCATTTAAGGCTGAGGCATTTTGGTTTTCTGCTGGCTTTTA2590.11849479583666933No Hit
TTCTTGCGGGATTTGTTATTTGGTACAATCTCAGTGGAACCAATTGTGCCCT2580.1180372869724351No Hit
GCCAGGGCAGGGCACCAAAGACTTTGAATGAGAGGACAGGTGTCATGGGACG2550.11666476037973236No Hit
TGCAATGGGTTTGTGTTGCTATGTACTACAGAAACAAATGAGCTGGATGAAT2550.11666476037973236No Hit
AAGCAGGGGAACATAGTCTTAAAATTACAGTTTGCTTGAATGTCTCAGAATA2540.1162072515154981No Hit
TGGAATGGGGTTTTCACGAGAGTTGTAATGAATTTGTAAATCACACTGAAGA2530.11574974265126388No Hit
ACCAGCGGGCAGAGGCTGGGCCTGAACAGGGAGGTGGTCGCTCAGGCCTGGT2530.11574974265126388No Hit
AGTGCCGGGATACCAAGTCCTTCGTGAAGCACTCCATGGAGCATGTGTCAAT2520.11529223378702962No Hit
GTGGCCGGAGAGGTATGGATTAACTTAGGTTTTCGGACGTTAAGTTTTTCTG2510.1148347249227954No Hit
ACTACCGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTGG2500.11437721605856113No Hit
GTTAATGGGGGCCGGGCTTGTCTCGAAATCCTGACCTCCTGAAGGTCAGGAC2500.11437721605856113No Hit
TGGAGTGGGAATTTACATTTTTGATAAACAATGTCAGAAATTACTTTTATCT2500.11437721605856113No Hit
ACGTTTGGGGGAACAGCAGAGCCTGCTCCTTGCGTACCACAGGGATTAATCC2490.11391970719432688No Hit
GACACTGGGATTCCCCCATTGTCCCCTCCTTTTCCATACAGTGTATCTGATG2480.11346219833009265No Hit
CTGTACGGGAGCTGGTGCAGTGGTTCTGGCACACGCTGGAAGAGTTCTCCAA2480.11346219833009265No Hit
CGCCCAGGGGTCTATTTGATCAGTTTCTGTAACCTGAAATATTGGGGAATAG2480.11346219833009265No Hit
ATGGCCGGGAAACTGACTTGATCGTGGTGGATAAGCTTTTTGATGTGCTGCT2470.1130046894658584No Hit
TTGCTGGGGATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTC2470.1130046894658584No Hit
GAATAGGGGAAGTAATATCTAACTCAAAATCCAAGGTAGAACATAGATAAAA2460.11254718060162416No Hit
CAATATGGGATTTTAGATGCCATTTAGAACTTTTGTGATTCATTGGACGAGG2460.11254718060162416No Hit
GTGCATGGGTAAGTATTTAAATGTATTAAATTTCATTAAGATTAAACATGTA2460.11254718060162416No Hit
GATTCTGGGTAACAGGTTCCAATGCATTCACACAATGGAATACTACTCAGCA2450.11208967173738991No Hit
AAAATGGGATGTCCACTTATCAACAAGAAATTGTAAGACATACAAAGAAACT2450.11208967173738991No Hit
AATGCAGGGTCCCTAGCCTCATAGAGCATATACTCCAGAGGGGGAAGACAAA2440.11163216287315567No Hit
ATAGTGGGGACCAAACGCTACTGGTTCTTTTACATATGCACTACCTAATATA2440.11163216287315567No Hit
TTGCCTGGGGAATGGCCATAATCAAAAAATCAAAAAATAATAGATGTTGGCA2430.11117465400892143No Hit
CTCTTAGGGGACGCTCTTCCGATCTCAGCAGGGGATGTGGACATTGCCATCC2430.11117465400892143No Hit
CGCTATGGGACTGTTTTCCAGAGTGGCTGCATTATATTGCATTCCCACCAAC2430.11117465400892143No Hit
TTATGGGGGCTCTTCCGATCTTCTGCAGGGGACACAGGCAAGGAATCACAAC2430.11117465400892143No Hit
GTCGATGGGATAGAAAGTATACTCTAAAGGGAATTTGCCGAAGACCTTTTAC2420.11071714514468717No Hit
GTCATTGGGAATGAATATGGAAAAGCTTTCAGTCCCACCTCACGACCCACTG2420.11071714514468717No Hit
CAGCCTGGCCAACAAGGTGAAACCCTGTCTCTACTGATAATACAAAAATTAG2400.10980212741621868No Hit
TATTTGGGGCATCTATAAAGGTTACCCCTTCCTCCGCCAAAAAATGGCTTCT2400.10980212741621868No Hit
ACTGACGGGATAGGATTCAGTATCCAGAGTTTATTTTTAATCTTAATCCTCA2400.10980212741621868No Hit
TAAGTGGGGGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGT2380.1088871096877502No Hit
GTTAAAGGGAGCTATGAATTCTGGGAAGTCAATGTGAAAAACATTGCTGCAT2380.1088871096877502No Hit
ATAAGTAGGGGTAGAGAAGCCATGGAGAACAGACTTGAAAAGTTTAGGAAAT2370.10842960082351595No Hit
TTGCAAGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGTT2350.10751458309504747No Hit
GGGTACGGGATTACTTACTGTTAAAACTGTACCTTTTGCGATTTCACAGTTG2350.10751458309504747No Hit
AGCAATGGGCAAAACCACTTAGAAAACTAGAATAGAGAACCATAACATGTAC2350.10751458309504747No Hit
TCTAACGGGGCTCTAGGATATACATCTTGGAGAAATGCTGGCATATATTCAC2340.10705707423081322No Hit
ATGCACGGGACCTTTTGTTTGCCCGACACAATGTTTTGAAAAACTGAATCAA2340.10705707423081322No Hit
TCTGGGGGGGTCGCAGGGAGCTCCGCAGCGACGTGGACCAAGGTGTATGTGG2340.10705707423081322No Hit
GTGTCAGGGAAGGCTGAAAGCCTCAAGTGAAGGATCTTGCAGCTTGTAAAAG2340.10705707423081322No Hit
TGATGTGGGGGTTATTGAGCACTTAAATGTGGCTAGTTTGATGGAGGAGCTG2330.10659956536657898No Hit
ACATTAGGGATGACGGTTGTAACTACTAAACCAAATTTAGTTTTCACAGCAT2320.10614205650234473No Hit
TCATAGGGGAAGCTGGAATGTCATCGCATTTCCAAGACTAACTCAGTGTCCA2320.10614205650234473No Hit
CAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2310.1056845476381105No Hit
GCGGCCTGGGACCCTGGATGGACTGACTGGTGTTTCCCAGGACCACATGCGG2300.10522703877387625No Hit
AAACACGGGCAGTAGTCATGATTTAAAGTTATGAAACCGCCATTGCAAAATT2290.10476952990964199No Hit
ACATAAGGGAAGTTTGGGTGACTGGTGCAGAGTTACTTCCTAAAAGGCCACT2290.10476952990964199No Hit
CATCGAGGGAACCTGAGTTCAGTCACTTAATACCGAGGTCCTGCGCTCTGCT2270.1038545121811735No Hit
AATTTAGGGCCGATCCGTGAAGGGGGACTCCAATTCCTGCAGTTCTTTTCAC2270.1038545121811735No Hit
AACGTGGGGCTACTGTTACAGTTACACTACTAAATGCTGATAACTATCATCA2270.1038545121811735No Hit
TGTCAGGGGACGGTCTTCATATAATTAATTGAAAACACCCCTGAGCAAAGTG2270.1038545121811735No Hit
GTTGTGGGGGTGCAAGCTGCTTATCTAGAATACTGCAGTCCTGAGGAATTTC2270.1038545121811735No Hit
CTAGCAGGGGAGCTTACCAGTGATCTTCTGATCTTCAAGAAGACTAAGTTTG2270.1038545121811735No Hit
AACGGTGGGGCAGTTGGACACATATATTTTTTCTTGGAAGATGTATTTCCCA2260.10339700331693927No Hit
GCATATGGGTATATTCTAAATTTTCAAACTGTGATGTTTATTTTATACTTGT2260.10339700331693927No Hit
GCTCTGGGGCCAGTTCAAAAATGGAGAATACTTCGCCTAAAATACTGTTAAG2260.10339700331693927No Hit
TCTTGTGGGGAACTGTTCGATAAAACAAGAAAAATAGAAGCACGAGTGTCTG2250.10293949445270502No Hit
GTTCATGGGATTTATTCATATCATGCCTTATGGAAATTTCTTTTTCTGTATT2250.10293949445270502No Hit
ATTGCCGGGAGTGGAGGTCCAGTACTTCCAGCATCTGACTTTTTACAGGCCA2250.10293949445270502No Hit
TACAATGGGGAAGTGAAGTCATTATTGAGTCATGAATGGAGTGTAAGTGTGG2240.10248198558847077No Hit
AGTTAAGGGGATCTGTGACATATGGGTTATATTATTGTGTCTTTGTCAAACA2240.10248198558847077No Hit
AAAGAGGGACAGAAACAATAGCTGATACATTGCAGTAAAGTGTTCATCTTAG2240.10248198558847077No Hit
AGAACTGGGGACAACACTTATTTTCAACACCTGCCATTGCTGTTGCTTTTTC2240.10248198558847077No Hit
AAAAGCGGGGACCTGAAGGCCAAAGGGAAAAGTTGTGGAAGATGAGCTCAGA2240.10248198558847077No Hit
GTGAAGGGGAATCCAAATTCTACAATGGATAAGTATGATTTTAATAAATAGA2240.10248198558847077No Hit
TCTCCGGGGTCTGCAATGGGGATGTCCTGATAAGTCAGGAACTTCACTTCTA2240.10248198558847077No Hit
GAGGCTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC2230.10202447672423653No Hit
TCTCAAGGGAGAAGGAAAATTGGAAAATACACAGATATGTAGAAATTAAATA2230.10202447672423653No Hit
ATTAACGGGATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTT2220.10156696786000229No Hit
TGAGCAGGGACCAAAGCAATTCCCCATGCTAAAACCTTAAATTAACACAGAA2210.10110945899576805No Hit
CAGTGCGGGCTATCCATGGAGGTGTGACTGGCACTAGCCTAGCCCTCCAAAA2210.10110945899576805No Hit
GTTGCAGGGATGAAAGAAAAGGCACTTCTTGGACAAGCACTGAAATATAATG2210.10110945899576805No Hit
GTTAGACCGGGAAGCTCTTTGAGGACAGGATTTGTGTCTGTCTTTGAATCTC2210.10110945899576805No Hit
ACTTTTGGGGTGCAAAGCTTATTTTCTTTTATCTGGGCAAAAATTAAAACAC2200.1006519501315338No Hit
AAGATTGGGGGGCCCAGACTGTTGCCCACTCCATATTCCAAAAGTAGGGGAG2200.1006519501315338No Hit
TCATCCGGGATGGCACAGTTTTTGTCCTGGTCCTCAGTGGAAGCTGGCTTTT2200.1006519501315338No Hit
TTCACTGGGAGGACGAGAGCCCACCTGAATCCTGTGAGCTGGGTAAGTGAGG2200.1006519501315338No Hit
GGCATAGGGCAATTTTAAGTGGTAAGACATTTACTGGGACACAGAGTAAAAT2200.1006519501315338No Hit
TTGCCAGGGTGGTCTGTCCTTTTAACTGCTTCTAAGTGAACGAAGTGAGGAG2190.10019444126729954No Hit
GCACAGGGGCAGTCAGGGACATACTTTGTGCAATATTTCTGTGATTGTGCCT2190.10019444126729954No Hit
GGTTTGGGAGCTTCATTTTCTACTAGCACCTATCATAATGGTCTTAGTCATT2190.10019444126729954No Hit
TCGCTAGGGAAGTGTTTTAACCTTTTATCTTTTCCAGGCTCTTAGTCATCTT2190.10019444126729954No Hit
CACTGGGGGCATATTCTAGGCAAAGGAACTTAAAACTTCTTGGAGGGAAAAT2190.10019444126729954No Hit
GTCTCAGGGTACTGAAGTTCGTGCGCAACGTGCCCTGGGAATTTGGCGACGT2190.10019444126729954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)