FastQCFastQC Report
Sat 31 Dec 2022
SRR5846113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101318
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC8070.7965021022917941No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT7590.7491265125644012No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG4720.46585996565269744No Hit
GTCACAGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT3810.37604374346118163No Hit
GCTTTTGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT3650.3602518802187173No Hit
GTGGAGGGGCAGTCCTGCTCGGAAGCAGATGATGTGAAATGTTCCTTTCCAG3570.35235594859748515No Hit
TTAATAGGGCGGCAGGGCATGACCTGGGGAGGGCAGGTGGAAGCCGATGGCT3210.3168242563019404No Hit
CGGACTGGGAGCAATAGGATTTGCTGGTGAATTGATGTAGGTGTGACAGAAT3140.3099153161333623No Hit
CATTGGGGTGACACCGTGGCTTTAAGGCATGGCCCATGATGTGTCTCCGAGT2980.294123452890898No Hit
AGATGTGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA2950.2911624785329359No Hit
CGTGGGGATACTACTGTTCATAAGAAGAAATTACTTTCTAAACAGTAACTGA2950.2911624785329359No Hit
GCCTGCGGGCTCTGACTGGGATGGATCAACTCAGTTCTGTTATACATATTTA2950.2911624785329359No Hit
CCTACTGGGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2870.2832665469117038No Hit
AACAAAGGGAGGGAGCTTCTGGCCCAAGGCCGTCTGAGGGATCTGCTCCTTA2850.2812925640063957No Hit
CACAGGGGAGAACTCAAGATTGAAAGGTCCACTCAAAACCACACAGCTACTT2840.28030557255374167No Hit
TCTAATGGGAGAGCAGTGCTGGAATAGCATCTCCTTTCACTTTCCCAAAACT2830.27931858110108765No Hit
TTTTGGGGGAAGTGTTCAAAACTCTGCAGCATTAAACAACGTGTATGCAAAT2830.27931858110108765No Hit
TATGGGGGGTGGCTGCTCATTGGCAATGCTATCAACTATAGATTGCTTGCAA2670.26352671785862336No Hit
ACCAAAGGGAACATAGAGGGCATGGCAACTCTCTTTGACAGTGGTACCCCAT2670.26352671785862336No Hit
CGCACTGGGAACATTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACT2650.2615527349533153No Hit
TGCAACAGGGCAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGACCTT2650.2615527349533153No Hit
CATATAGGGATGTAGATGTGTGATGGACCTAGACATTTTCATGTTTCAACAT2640.26056574350066125No Hit
TGGTATGGGGTTGGAAACCTAAACTTTAACAAATCTGCTCCTGAATTAAAAA2560.2526698118794291No Hit
CACACGGGGAAGGAAGAAAACCAAATGGATATTGTCTTGGACTTCTCCCTAG2490.24576087171085098No Hit
TACCCAGGGGTTCTAGAAAGTGTTAGGATGTGTAGAAACTAGAAATCTTGCG2480.24477388025819696No Hit
GCTTTAGGGAAGTCATGAGTCTAATTCCCCTGGGACATAGCTAGCCTTCACA2470.24378688880554292No Hit
CTTACTGGGGACTTCCAACCAAAGCCTTTGAGTATGCACTAAATAGGTGAGA2420.23885193154227283No Hit
TCTCCAGGGATGAATGGCTCCACGAGGGTTCATCTGTCTCTTACTTTTAACC2360.23292998282634872No Hit
TACACCGGGGAGATTGAGTATCCTTTATCCAAAATGCTTAGGACCAGAAGTG2320.22898201701573262No Hit
GTGCGAGGGCAAGGATTTTCGTATAATATTGTTTCATTTAATTCTCATCACG2300.22700803411042464No Hit
TGTTACGGGGGGTTCACTCATTCTCAGATTAAATTGCCACTTAAAGAAATAA2290.22602104265777057No Hit
ACTAATAGGACTGTCAGGTGCTTCTGTTGCCATTTCTGGATGCTCGTTCTTT2280.22503405120511658No Hit
TCAGAGGGATTAAAACTATTTACTGTTAAAAAATCTGTGACTTCATGAAGTT2280.22503405120511658No Hit
TAGATCGGGAACATCCATGTGACTAGTGTATATGGTATTTGCTCTCCATTAG2230.22009909394184649No Hit
AATGCTGGGATCACTGAATCCTCACTCTCATTTTCTGAAGTAGTGTACCAGT2230.22009909394184649No Hit
TTCCGCGGGGCATGGTCACAGTAGAGATCCCCGCAACTCGCTTGTCCTTGGG2190.21615112813123039No Hit
AATAATGGGCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTACGA2140.2112161708679603No Hit
GTATCCGGGTTTGCCTCAGACAGGTATCTCTTCGTTATCAGAAGAGTTGCTT2130.21022917941530628No Hit
TGAAGGGGCGCTCTTCCGATCTACTCATGGGTATTCATGTAAGTCATATTTT2090.20628121360469018No Hit
CTCACATGGGAGACCGAGAAGTCTCGCGATAGCCAGCCGCGGCTGCCCTTGC2090.20628121360469018No Hit
TTTTTCGGGATGGATTATTAAAGTTGTCTACTCAAGTATAAAATATATGCAA2080.2052942221520362No Hit
ATAACTGGGCGATCTAGGTTAGGGATTCACAAATTTTCTCATGCTCGTTTCT2060.20332023924672812No Hit
GCCCAGGGGGATCTCTTAGCACATTTCTTAGGATTAAGGGTCCAAAAATGCT2060.20332023924672812No Hit
TCGTCAGGGAATGGTAGGTATTACTTACCAAATGACACAATACCTTTTTGAA2030.2003592648887661No Hit
TCAAAGGGGAAGCCACTGCTTCCTGGATGACAGCCAGACCCTGTCTCAAGAA2020.19937227343611202No Hit
GAGACGGGGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGC2000.197398290530804No Hit
ACACATGGGGTTGAAAAGACTGAAAGTTTAAAACATGAAAAAAATCAATATT1970.19443731617284193No Hit
ATGTTAGGGATGTATTAACAGAAAGATCCCAGAATTGTTGAGGGGGGATCAA1930.19048935036222586No Hit
AATCTAGGGTGTCCAGATCTGTTTATTCTTTTACAGTATTAAATGATTTTCA1920.18950235890957184No Hit
AGCTGGGGGATTGCAAAGTTGTTTATCTTCTATTTCTCCCAGGGACTATAAG1910.18851536745691783No Hit
GTGTCTGGGCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG1890.1865413845516098No Hit
ATGCCTGGTAGGGAAAGGATACTATATTTAACAATGCTGCTGGGATAATTGG1840.1816064272883397No Hit
AGCAGTGGGCATAGGCTTTTGAGTGCCTGAATTTCAGTTCATTCTGTAATTT1840.1816064272883397No Hit
ATTCTTGGGATCGCTGGATGCTGGTGGTATGATGGCAAGATGGGAACAGGTA1820.17963244438303164No Hit
CTGTATGGGAGAACTAAAAGCAGAAATAAAAAATTATAGAAAATTAGAAAAT1810.17864545293037762No Hit
GTAAGCGGGAAAGAAAAAAATCTACAGCATTTTTCAGACTCCACCCTAGAAA1800.1776584614777236No Hit
AGTGACAGGGCTAATTAATTTGCTTTATACATTTTCTTTTACTTTCCTTTTT1750.17272350421445348No Hit
TTGTACGGGGCATTATATGTGTGTTGGTTGTGGATTTTGTTTCTAAAATTGG1750.17272350421445348No Hit
CGACTGGGAGAGGCGGGCAGATCACCTGAGGTTAGGAGTTTGAGACCGGTCT1740.17173651276179946No Hit
TTTATTGGGAAGGAGGCAGACTGATGGCGATTCCCTCTCACCCGGGACTCTC1640.16186659823525928No Hit
TGCGAGGGGATAGAAGGAACATGTGTCAGCATAATAAAAGCCATATACTACA1610.15890562387729723No Hit
CGATTAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC1610.15890562387729723No Hit
TCGTCAGGGAAAGCAAACTGGGGAGGTGGGCATTAGTATCTCTGTTTTGCCA1600.1579186324246432No Hit
ATCTGTGGGATTCTAAAAGATGGCAAAACATAGGAACAAAATACAGATCAGT1600.1579186324246432No Hit
TCTAGAGGGAGTTACAGCAGGTGATTCAGCTACTTGAGGTCGGTAACAGACC1580.15594464951933515No Hit
AAACTTGGGCTCATTCAGGTCTTAGATTGTAGTTAGCATTCCTATCTGTATG1560.1539706666140271No Hit
GAAAAGGGGACAGTGACGAAAGTGTCTACGGTCTGTGAAGCTGCTGCCCCTT1550.1529836751613731No Hit
TCTATGGGGATACACTCTTCTCACTTGACATTTTAGCTATATAATATGTACT1550.1529836751613731No Hit
TTCAGCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC1550.1529836751613731No Hit
GTCAAAGGGACACGGTACGTTTATGTTCAGTTAGATGACCAAAGCTTCTTAT1520.15002270080341104No Hit
TCTACTGGGATACAAAAATTAATGCAAGGTGGATTAAAGATTTAATTGTAAG1470.14508774354014095No Hit
AAACGCGGGATTCCTCCATTGAGAGAGAGGCTCTATGGCCGATTAACCCAGA1450.14311376063483291No Hit
TATGTAGGGTTATGTATTTGTAGTTTTTACTCTCCTTATATTTTTCCTATCG1440.14212676918217887No Hit
ACGTTTGGGTATGTGTAACTTCCCCCCAACCCCAAGCTGTTTCGGAAGATAT1440.14212676918217887No Hit
CTCTGGGGGACTCTTGATCCTGCCCTTGCTGCACTTGGGCTTCCTGGAGCAA1430.14113977772952485No Hit
GCCCGAGGGAGTTGGGAACCTCATTAGAAATGACCTCAGCTGCCCCATATCT1380.13620482046625476No Hit
GCAGCAGGGCTTCGCTGACGCCATAAAACTCTTCACCAAAGACCCCCTAAAA1360.13423083756094673No Hit
TTAACAGGGCATTGTTTCCCTTTAAAACAAAAAACAAATCAGTACCCCTGCC1360.13423083756094673No Hit
AAGCACGGGACTGCTTCCAGGCTCCAAATGCGCCTTTCAATAAAGTAGTTCC1340.1322568546556387No Hit
AATTAGGGCTGTCCTTCCGCTCTACCATCCTAGTGCTTGGCACCCAACCTCC1330.13126986320298467No Hit
TATACAGGGGATGAGGGGCTTCTACATTAGGAAAAGCATCCCAGTGCAGAAC1310.12929588029767664No Hit
AATTCTGGGGGCACAAACCCGCATGTATGACCATCGGCCTTCAAAAACCCTG1290.12732189739236857No Hit
TTTTGGGGAGGAATTCGAAGACCTTGGCTAGGTGGTGAGCTTGGAAGAGCTG1290.12732189739236857No Hit
AGTTGGGCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACT1280.12633490593971455No Hit
CATGTGGGAAGTCAGTGATGGAACCTGCTTTATGACCAAGATTCATCCTCAA1250.12337393158175251No Hit
TGAACTGGGGAAAATCATCATGCAACACATGAAAAAAACATTAATCAGAAGT1240.12238694012909848No Hit
TAAGGTGGGAGTCCAGTTTAGTACTTTTTCTTTTCGTGTATTCAAGGTTAAA1230.12139994867644446No Hit
TGATTGGGGATTATTCAGAGTCTTCAGCAAGGACATTGTATTTTACTTGTGT1230.12139994867644446No Hit
AAGTGTGGGATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1230.12139994867644446No Hit
GTTCAAGGGCTTTCAGGTGAACCCAATTCAGTGTCTTTAGACCACATTTTCA1230.12139994867644446No Hit
GACGTAGGGCGTATGTTTTGGAGTAGTGGAATTGCCAGCCAGGCCCTGTGGC1220.12041295722379043No Hit
GTTTTTGGGACCTGAAACCGGAAACTCCTGAGAATAAGGTTGATTCTACACA1180.11646499141317436No Hit
GTGAACAGGGCTAAGTAAACGATTAAATGGTAGTGGGGAGCCTTCCAGGAAG1180.11646499141317436No Hit
TGCATAGGGGATATAGTATCTTTATATAATGAGTGAATAAAATCAGAGTAAC1180.11646499141317436No Hit
TCATATGGGTGACTACCCAAACTGTATTTTACCAATAAATATGCATATGATC1170.11547799996052034No Hit
GAACAGGGGTGGCTTAAGGCCATTCAGAAGCCAAGAAGTAGGAGTGGTGCAT1170.11547799996052034No Hit
TAGCGGGGGAACTAAGTGCACCTGTGATCTTGGCCAAAAAACCATTGCAACT1160.11449100850786631No Hit
TATAGAGGGCTCTCTTATTTACACACTACCCATGTACACACCCTCTTCAGCC1160.11449100850786631No Hit
GTGTTAGGGAGATTTGAGACAACTAGCCTGTTATTCAGCCCAATATCCCCCC1150.11350401705521232No Hit
ATACATGGGAGAAGCTCTCCGGGCCCTCAGGGGCTTAGATCCCACTTGAGTC1140.11251702560255829No Hit
CTGTATGGGAGTACAGTATATTGTTATGTACATATCATTGTTAATACAGTCC1140.11251702560255829No Hit
CGAGTAGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAG1140.11251702560255829No Hit
CGGATGGGGAAGGCTATTGAATGACCAGGTGGGGTAGCAGAGAGGAACACTG1110.10955605124459622No Hit
ACTGTAGGGGAATGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGA1110.10955605124459622No Hit
AAACTAGGGATGAGCAGTTCTCTAAAAACATAAAGCTTGGAATACACGAAGA1110.10955605124459622No Hit
AATGCGGGGACACCATTGTTCCTTTCACACTTTCCTTTGTTGCATGCAGTTG1100.10856905979194219No Hit
CTGCGCGGGAGACTGTCTAAGGTATTTTTTTCCCCAAACACTTGAATCTTGG1090.10758206833928818No Hit
TCAAAAGGGACATGTTTGTGAATATTTTATAATGTGGAATGATCATTGAAAT1080.10659507688663417No Hit
CTGTCCGGGCTTCCGATCTATGTAAGGGATATCAATCAATCAATCAATCAAT1070.10560808543398015No Hit
AATGCGGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAA1060.10462109398132612No Hit
ACCACCGGGAAATTGGCAAGATGTATTTTTACTTTCCAAGATGCCGTGTGTG1060.10462109398132612No Hit
TGCCTTGGGTGCTAGAGCTATTTTCCTGAAATACATTTGCAAAATAAGGCTG1050.1036341025286721No Hit
GCGCTTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1050.1036341025286721No Hit
TGCACCAGGGGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGA1050.1036341025286721No Hit
CTGTTAGGGAGAAACATAGATGTTTCAATCCAGAAAAGCCTTTCTATTTTTT1050.1036341025286721No Hit
CTGGCAGGGAATGTACCAGACCCTGGGGGGGGTCACTGGGCCCTAGATTTTT1050.1036341025286721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)