FastQCFastQC Report
Sat 31 Dec 2022
SRR5846111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99973
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT8080.8082182189191082No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC7550.7552039050543646No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG4660.46612585398057477No Hit
GTCACAGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT3780.37810208756364216No Hit
GCTTTTGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT3290.32908885399057747No Hit
CGGACTGGGAGCAATAGGATTTGCTGGTGAATTGATGTAGGTGTGACAGAAT3220.3220869634801396No Hit
GTGGAGGGGCAGTCCTGCTCGGAAGCAGATGATGTGAAATGTTCCTTTCCAG3170.31708561311554123No Hit
CGTGGGGATACTACTGTTCATAAGAAGAAATTACTTTCTAAACAGTAACTGA3140.3140848028967821No Hit
AGATGTGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGA3070.30708291238634433No Hit
CATTGGGGTGACACCGTGGCTTTAAGGCATGGCCCATGATGTGTCTCCGAGT3050.30508237224050494No Hit
TTAATAGGGCGGCAGGGCATGACCTGGGGAGGGCAGGTGGAAGCCGATGGCT3020.30208156202174585No Hit
CCTACTGGGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2840.2840767007091915No Hit
CGCACTGGGAACATTCTACTACTCACTCTCACTGCCCAAGAACTATCAAACT2830.2830764306362718No Hit
AACAAAGGGAGGGAGCTTCTGGCCCAAGGCCGTCTGAGGGATCTGCTCCTTA2820.2820761605633521No Hit
TCTCCAGGGATGAATGGCTCCACGAGGGTTCATCTGTCTCTTACTTTTAACC2810.2810758904904324No Hit
TCTAATGGGAGAGCAGTGCTGGAATAGCATCTCCTTTCACTTTCCCAAAACT2780.2780750802716734No Hit
TATGGGGGGTGGCTGCTCATTGGCAATGCTATCAACTATAGATTGCTTGCAA2770.27707481019875363No Hit
CACAGGGGAGAACTCAAGATTGAAAGGTCCACTCAAAACCACACAGCTACTT2760.276074540125834No Hit
ACCAAAGGGAACATAGAGGGCATGGCAACTCTCTTTGACAGTGGTACCCCAT2750.2750742700529143No Hit
CTTACTGGGGACTTCCAACCAAAGCCTTTGAGTATGCACTAAATAGGTGAGA2690.2690726496153962No Hit
CACACGGGGAAGGAAGAAAACCAAATGGATATTGTCTTGGACTTCTCCCTAG2670.2670721094695568No Hit
GCCTGCGGGCTCTGACTGGGATGGATCAACTCAGTTCTGTTATACATATTTA2670.2670721094695568No Hit
TGCAACAGGGCAAGTTATGCCTTCTGGCTACTACAGAATCTCCGCAGACCTT2610.26107048903203867No Hit
CATATAGGGATGTAGATGTGTGATGGACCTAGACATTTTCATGTTTCAACAT2490.2490672481570024No Hit
TTTTGGGGGAAGTGTTCAAAACTCTGCAGCATTAAACAACGTGTATGCAAAT2480.24806697808408268No Hit
GTATCCGGGTTTGCCTCAGACAGGTATCTCTTCGTTATCAGAAGAGTTGCTT2460.24606643793824334No Hit
TCAGAGGGATTAAAACTATTTACTGTTAAAAAATCTGTGACTTCATGAAGTT2440.24406589779240395No Hit
GCTTTAGGGAAGTCATGAGTCTAATTCCCCTGGGACATAGCTAGCCTTCACA2430.24306562771948428No Hit
TGGTATGGGGTTGGAAACCTAAACTTTAACAAATCTGCTCCTGAATTAAAAA2430.24306562771948428No Hit
ATGTTAGGGATGTATTAACAGAAAGATCCCAGAATTGTTGAGGGGGGATCAA2360.23606373720904644No Hit
TAGATCGGGAACATCCATGTGACTAGTGTATATGGTATTTGCTCTCCATTAG2350.23506346713612677No Hit
TACACCGGGGAGATTGAGTATCCTTTATCCAAAATGCTTAGGACCAGAAGTG2290.22906184669860863No Hit
TTCCGCGGGGCATGGTCACAGTAGAGATCCCCGCAACTCGCTTGTCCTTGGG2270.22706130655276927No Hit
GTGCGAGGGCAAGGATTTTCGTATAATATTGTTTCATTTAATTCTCATCACG2220.22205995618817082No Hit
GTAAGCGGGAAAGAAAAAAATCTACAGCATTTTTCAGACTCCACCCTAGAAA2180.21805887589649206No Hit
TGTTACGGGGGGTTCACTCATTCTCAGATTAAATTGCCACTTAAAGAAATAA2180.21805887589649206No Hit
ACTAATAGGACTGTCAGGTGCTTCTGTTGCCATTTCTGGATGCTCGTTCTTT2170.21705860582357234No Hit
TCGTCAGGGAAAGCAAACTGGGGAGGTGGGCATTAGTATCTCTGTTTTGCCA2140.21405779560481328No Hit
TACCCAGGGGTTCTAGAAAGTGTTAGGATGTGTAGAAACTAGAAATCTTGCG2100.21005671531313455No Hit
CTGTATGGGAGAACTAAAAGCAGAAATAAAAAATTATAGAAAATTAGAAAAT2080.20805617516729516No Hit
AATGCTGGGATCACTGAATCCTCACTCTCATTTTCTGAAGTAGTGTACCAGT2050.2050553649485361No Hit
CGACTGGGAGAGGCGGGCAGATCACCTGAGGTTAGGAGTTTGAGACCGGTCT2040.20405509487561643No Hit
AGCTGGGGGATTGCAAAGTTGTTTATCTTCTATTTCTCCCAGGGACTATAAG2030.2030548248026967No Hit
ACACATGGGGTTGAAAAGACTGAAAGTTTAAAACATGAAAAAAATCAATATT2030.2030548248026967No Hit
TTGTACGGGGCATTATATGTGTGTTGGTTGTGGATTTTGTTTCTAAAATTGG2030.2030548248026967No Hit
GCCCAGGGGGATCTCTTAGCACATTTCTTAGGATTAAGGGTCCAAAAATGCT2010.20105428465685737No Hit
CTCACATGGGAGACCGAGAAGTCTCGCGATAGCCAGCCGCGGCTGCCCTTGC1980.1980534744380983No Hit
CGATTAGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC1970.1970532043651786No Hit
GAGACGGGGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGC1960.19605293429225892No Hit
ATAACTGGGCGATCTAGGTTAGGGATTCACAAATTTTCTCATGCTCGTTTCT1950.19505266421933923No Hit
TTTTTCGGGATGGATTATTAAAGTTGTCTACTCAAGTATAAAATATATGCAA1950.19505266421933923No Hit
TGAAGGGGCGCTCTTCCGATCTACTCATGGGTATTCATGTAAGTCATATTTT1940.19405239414641953No Hit
TCAAAGGGGAAGCCACTGCTTCCTGGATGACAGCCAGACCCTGTCTCAAGAA1930.19305212407349984No Hit
AGCAGTGGGCATAGGCTTTTGAGTGCCTGAATTTCAGTTCATTCTGTAATTT1910.19105158392766047No Hit
GTGTCTGGGCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG1910.19105158392766047No Hit
TTTATTGGGAAGGAGGCAGACTGATGGCGATTCCCTCTCACCCGGGACTCTC1890.18905104378182108No Hit
AGTGACAGGGCTAATTAATTTGCTTTATACATTTTCTTTTACTTTCCTTTTT1890.18905104378182108No Hit
ATTCTTGGGATCGCTGGATGCTGGTGGTATGATGGCAAGATGGGAACAGGTA1810.18104888319846357No Hit
TCTAGAGGGAGTTACAGCAGGTGATTCAGCTACTTGAGGTCGGTAACAGACC1780.1780480729797045No Hit
AAGCACGGGACTGCTTCCAGGCTCCAAATGCGCCTTTCAATAAAGTAGTTCC1760.17604753283386515No Hit
AATCTAGGGTGTCCAGATCTGTTTATTCTTTTACAGTATTAAATGATTTTCA1750.17504726276094545No Hit
TCGTCAGGGAATGGTAGGTATTACTTACCAAATGACACAATACCTTTTTGAA1690.16904564232342734No Hit
ATGCCTGGTAGGGAAAGGATACTATATTTAACAATGCTGCTGGGATAATTGG1620.1620437518129895No Hit
AAACTTGGGCTCATTCAGGTCTTAGATTGTAGTTAGCATTCCTATCTGTATG1620.1620437518129895No Hit
TAAGGTGGGAGTCCAGTTTAGTACTTTTTCTTTTCGTGTATTCAAGGTTAAA1610.1610434817400698No Hit
TCTATGGGGATACACTCTTCTCACTTGACATTTTAGCTATATAATATGTACT1610.1610434817400698No Hit
AATAATGGGCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTACGA1510.15104078101087293No Hit
ATCTGTGGGATTCTAAAAGATGGCAAAACATAGGAACAAAATACAGATCAGT1480.14803997079211387No Hit
GCCCGAGGGAGTTGGGAACCTCATTAGAAATGACCTCAGCTGCCCCATATCT1460.1460394306462745No Hit
CTGTATGGGAGTACAGTATATTGTTATGTACATATCATTGTTAATACAGTCC1460.1460394306462745No Hit
CTCTGGGGGACTCTTGATCCTGCCCTTGCTGCACTTGGGCTTCCTGGAGCAA1440.1440388905004351No Hit
TCAAAAGGGACATGTTTGTGAATATTTTATAATGTGGAATGATCATTGAAAT1430.14303862042751544No Hit
TGCGAGGGGATAGAAGGAACATGTGTCAGCATAATAAAAGCCATATACTACA1410.14103808028167605No Hit
AAACGCGGGATTCCTCCATTGAGAGAGAGGCTCTATGGCCGATTAACCCAGA1410.14103808028167605No Hit
ACGTTTGGGTATGTGTAACTTCCCCCCAACCCCAAGCTGTTTCGGAAGATAT1390.1390375401358367No Hit
TGATTGGGGATTATTCAGAGTCTTCAGCAAGGACATTGTATTTTACTTGTGT1370.1370369999899973No Hit
AATTCTGGGGGCACAAACCCGCATGTATGACCATCGGCCTTCAAAAACCCTG1360.1360367299170776No Hit
GTCAAAGGGACACGGTACGTTTATGTTCAGTTAGATGACCAAAGCTTCTTAT1350.13503645984415794No Hit
AAGTGTGGGATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC1330.13303591969831854No Hit
GAAAAGGGGACAGTGACGAAAGTGTCTACGGTCTGTGAAGCTGCTGCCCCTT1320.13203564962539885No Hit
GTTCAAGGGCTTTCAGGTGAACCCAATTCAGTGTCTTTAGACCACATTTTCA1320.13203564962539885No Hit
TGCATAGGGGATATAGTATCTTTATATAATGAGTGAATAAAATCAGAGTAAC1310.13103537955247915No Hit
AATTAGGGCTGTCCTTCCGCTCTACCATCCTAGTGCTTGGCACCCAACCTCC1300.13003510947955946No Hit
TTAACAGGGCATTGTTTCCCTTTAAAACAAAAAACAAATCAGTACCCCTGCC1300.13003510947955946No Hit
GTGAACAGGGCTAAGTAAACGATTAAATGGTAGTGGGGAGCCTTCCAGGAAG1290.1290348394066398No Hit
TTCAGCGGGGAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGTAGC1280.1280345693337201No Hit
GCAGCAGGGCTTCGCTGACGCCATAAAACTCTTCACCAAAGACCCCCTAAAA1270.1270342992608004No Hit
TATAGAGGGCTCTCTTATTTACACACTACCCATGTACACACCCTCTTCAGCC1270.1270342992608004No Hit
TCTACTGGGATACAAAAATTAATGCAAGGTGGATTAAAGATTTAATTGTAAG1260.12603402918788073No Hit
TACTTAGGGACTGCATCCTGTAGACATGGAGGGTTTCCCCCCTTATTCTGGG1260.12603402918788073No Hit
GCTTTTGGGGACTTCCCAAGAAAGACAGGTACAAACAAGCATAAAATTGTGA1250.12503375911496104No Hit
TTTTGGGGAGGAATTCGAAGACCTTGGCTAGGTGGTGAGCTTGGAAGAGCTG1250.12503375911496104No Hit
AGTTGGGCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACT1250.12503375911496104No Hit
CATGTGGGAAGTCAGTGATGGAACCTGCTTTATGACCAAGATTCATCCTCAA1240.12403348904204134No Hit
GAACAGGGGTGGCTTAAGGCCATTCAGAAGCCAAGAAGTAGGAGTGGTGCAT1240.12403348904204134No Hit
TGAACTGGGGAAAATCATCATGCAACACATGAAAAAAACATTAATCAGAAGT1230.12303321896912167No Hit
TGCACCAGGGGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGA1220.12203294889620198No Hit
AGATTTGGGATCCCCAAACCCTTGTCATTTCCCCCAGTGAGCTCTGATTTCT1210.12103267882328228No Hit
TAGCGGGGGAACTAAGTGCACCTGTGATCTTGGCCAAAAAACCATTGCAACT1180.11803186860452322No Hit
TGCCTTGGGTGCTAGAGCTATTTTCCTGAAATACATTTGCAAAATAAGGCTG1160.11603132845868386No Hit
TATACAGGGGATGAGGGGCTTCTACATTAGGAAAAGCATCCCAGTGCAGAAC1160.11603132845868386No Hit
GTTTTTGGGACCTGAAACCGGAAACTCCTGAGAATAAGGTTGATTCTACACA1150.11503105838576416No Hit
TATTCAGGGAAGACTCTGCTCTTTCAAACTTCAGTTGCATATGTGAACTTAG1150.11503105838576416No Hit
TCATATGGGTGACTACCCAAACTGTATTTTACCAATAAATATGCATATGATC1140.11403078831284447No Hit
ACCACCGGGAAATTGGCAAGATGTATTTTTACTTTCCAAGATGCCGTGTGTG1140.11403078831284447No Hit
GACGTAGGGCGTATGTTTTGGAGTAGTGGAATTGCCAGCCAGGCCCTGTGGC1120.11203024816700509No Hit
TCGTAGGGGATAAGTGGGTTTATTGGTCTTTTCTAATTGTATAATTTAATTT1110.11102997809408541No Hit
GCGCTTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1080.10802916787532633No Hit
ATCAAGGGCACTACTTTAATGTTTGACTTCTTCACTTCAAAAATAGTTGAAA1060.10602862772948697No Hit
CGAGTAGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAG1050.10502835765656728No Hit
CTGGCAGGGAATGTACCAGACCCTGGGGGGGGTCACTGGGCCCTAGATTTTT1050.10502835765656728No Hit
AATGCGGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAA1040.10402808758364758No Hit
AAACTAGGGATGAGCAGTTCTCTAAAAACATAAAGCTTGGAATACACGAAGA1040.10402808758364758No Hit
CTTGGAGGGACTGGAGAATGCCCGAGGCCACCCCAAACTCCATGAGTTCAAC1030.10302781751072788No Hit
TATGTAGGGTTATGTATTTGTAGTTTTTACTCTCCTTATATTTTTCCTATCG1020.10202754743780822No Hit
ATTACAGGGGAAAAACTGGAATTCTAGACCGGGCACGGTGGCTCACGCCGGT1010.10102727736488852No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGT1010.10102727736488852No Hit
ATCTTAGGGCTAACCGAGGGATGTCAAGCACCTCTCATCACAGTCTAGTTCC1000.10002700729196883No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)