FastQCFastQC Report
Sat 31 Dec 2022
SRR5846109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43698
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC5631.2883884845988374No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT5521.2632157078127144No Hit
TATATGGGGATCCTGGCTACAAGGTGAAGCAGCGCCTTGGCGGGGGCCGTGG3810.8718934505011671No Hit
ACTATAGGGAGGAGGAAGCACTTGGTATCTGGTATGTGATCGCTGTAGAGAA3560.8146825941690696No Hit
GCAGTTGGGGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACG3360.7689139091033915No Hit
CTCGCATCGGGATATTTAAGTACAGTTTTCATGAGGAAGTTTTAAAAGACCA2920.6682228019588997No Hit
GCTCCTGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA2820.6453384594260607No Hit
TTCACTGGGTCCCCAAGATTGAGGCTATTGAGACATGGCACTATCAACAGGT2810.6430500251727768No Hit
TATAATGGGCAAGCAAAAACCAGGCTCCATCTACTCTTTGAAGCTTCTGCCC2730.6247425511465056No Hit
TCCTATGGGGTGGCAGTGAAGATGGATCCCTCCATGTCTTCGTGCTTGCTGT2610.5972813401070988No Hit
TATCTAGGGAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCCTTATC2600.5949929058538148No Hit
AATGTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2320.5309167467618656No Hit
GGTCTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2280.5217630097487299No Hit
GAAACCGGGACTGTGTGCCAAGCGCTTTTACGTGGCTTCTCCCTCAGCCAGC2240.5126092727355943No Hit
GCTTGCGGGAGGTGTTTGGTTATTGCTTTCAGAGTCAGACATTGTTTAGGTT2230.5103208384823104No Hit
TCCTGTAGGGGGGTTAGATCGTCAGGTGAGGGAGGAAGGGATAGCCAGCGCG2210.5057439699757426No Hit
TCACTGGGTGAGGCTGATGCTACGCTCAGGCACACAGATTTTGGCACCAGCT2200.5034555357224587No Hit
GGTACTGGGGTTTTATGAAACCATCATAACTTGACCCAAACCCCAAGAAAGA1910.4370909423772255No Hit
GAACCTGGGTGACAGAATAAGACCCTGTCTCAATAAATAAATAAAATTTCTA1860.425648771110806No Hit
CAAAAGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGG1850.42336033685752206No Hit
AAGCTTGGGAAGGCTAGGATTGAACTCCATATGCAGAGGGTTTCTATTCACA1820.4164950340976704No Hit
AAACAAGGGAAGTTCAAGGATCCCATATAGTATAAGAATATAATTTCACTTG1740.39818756007139916No Hit
GTATGAGGGATGGTGGGGCAATGGTTATTTGGTTATTTTACTCAATTGGTTA1680.38445695455169576No Hit
TAACGAGGGAGCCTCTTTGATGGATTTGCAGATGGGTTAGGAGTGGCTGAAG1640.3753032175385601No Hit
CGTAATGGGAGACTTAAAGATTTGAGAAATTATTTGTGTCATTTACTAGACG1630.3730147832852762No Hit
GCTAGCAGGCGACCAGGTGAACGGAGGCAAGGTGGTGAGGAAGCACTCAGGG1630.3730147832852762No Hit
CCTTCTGGGTATAACAAGTGTTGGAAGTTTGAGCATTGCAATTTCAACGACG1630.3730147832852762No Hit
AAACAAGGGGTCTTAAATTACTTTTGAATTTCTAATCTGTGAAGGCAGTAAA1620.3707263490319923No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGT1520.3478420064991533No Hit
GCTAGCGGGCGAAATCTACAATGTAATATGAGTGCATTGTATGGGTTTGAAA1510.34555357224586936No Hit
TGGCATGGGGTGTTACTGGAAAAATTAAGTTGTTGAAAGTAAGTTGTTTTCC1510.34555357224586936No Hit
ATGGCAGGGAGGTAAAGTCTACTTTTATTTTGTCAAGCTTAATAATTTGTGT1500.34326513799258546No Hit
GTAACTACCCAGCCAGCAAGTATATAGCACAGAACACTGTGTTACTTTACAA1450.33182296672616596No Hit
TACTCCGGGATCCCTAGAACGTTGAGGGCACGAGTCGGGTCCTGAGACCAGG1410.32266922971303036No Hit
GTTCTAGGGTCTGCCTTCTGAGTTGGCTCATCCTGGGCAGTCACAGACTGTC1400.32038079545974646No Hit
TGCATGGGGACTCGAGAATGAAGAGAATGTTTTCTATAGGGAACTGCTAGAG1310.2997848871801913No Hit
GCTTATGGGTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAA1290.2952080186736235No Hit
TTGACCGGGATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC1290.2952080186736235No Hit
TCCTAGGGGAGTGACATGCAGTTATATATTTTGTTTTTAAAAGGGGGGGAGC1260.2883427159137718No Hit
TCATTGGGTGTTGTGAAAAAGGAAGAGAATCATTAAAGAAAAAGAAAAAAGA1220.27918897890063615No Hit
TAAGAAGGGGTGTTACTGGAAAAATTAAGTTGTTGAAAGTAAGTTGTTTTCC1190.27232367614078445No Hit
GATTATGGGATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC1170.26774680763421665No Hit
AAGCGCGGCAGCTATCTCAGATTTGAGCTATGTGAACTTTTAGTCAAGATGA1150.26316993912764886No Hit
TGATACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1100.25172776786122936No Hit
GCATGCGGGGCTCGGACCTAGGTCGCGGCGACATGGCCAAACGTACCAAGAA1070.24486246510137763No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTA1010.2311318595816742No Hit
ACATCGTGGGTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCC930.212824385555403No Hit
GTGCGAGGGGCTACCCGTCCAGCCTGCCCAGACCCTCTCAGAGGAACGGGGT920.21053595130211908No Hit
ATATCTGGGTGCAGAAGAGTTTGTGGGTCTAGTGTTCAGTGAAAATGAAAAC900.20595908279555128No Hit
CTTTAGTAACACGGACAAGGCTCCTCGCCAAGGAACTCGTGGCAGAAGAGGG900.20595908279555128No Hit
GCTGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA900.20595908279555128No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCCTGTCTCT890.2036706485422674No Hit
GTTGGCGGGACTGAATGAATTTTGAAGCTTAATGTTTTTAAAGGCATAGTTT850.19451691152913175No Hit
GACATAGGGCTTCATCCTTATTACCATGTCTGAGAACCCCTATTAACTGGTA830.18994004302256398No Hit
CTAAGAGGGAACACATTTTGTATAATTGTATCGTATAGCTGTATTGAATCAT780.17849787175614446No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCCTGTCTCTTATAC750.17163256899629273No Hit
AGCCACGGTGCTATTCTGAGAGCCATTTTAAACTGCACTGCTCCAACCACCC740.16934413474300883No Hit
TCTACAGGGATTTAAAGCTATTTTATTTTTCTACGCCATCTGGCTAGTAGAT710.16247883198315713No Hit
ACGAAGGGTAGAAGAACAGACCTAACTAGCGAATGTATGAATGAAAATGCAT700.16019039772987323No Hit
GCCACTGGGGTCTATTGGTTATTGATCTTGCCATCTTTATTTAAAACCATGT670.1533250949700215No Hit
CGTTGGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTA660.1510366607167376No Hit
TTCGGCGGGCTTTACTCTTATGGTATTTAGTCAAAATACTGTTTTCCAAAGT630.1441713579568859No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC620.14188292370360198No Hit
TAGCTGGGAGAAAAGAAGAGGGAACATTTATTTAAAGGATGGAAAGACTGGG570.13044075243718248No Hit
TAAACCGGGCACCAGTGAACACAAAAGAAGTGACTGTTTGTAGAATATGGAT460.10526797565105954No Hit
TAACAGGGGGACGCGAAAATGACATGTCTCCAAGCAACAATGTGGTTCCTAT460.10526797565105954No Hit
CAAATGGGTTACCTCCAAATATGAAAATCTATAACAACCTATGGTTGAAGGA440.10069110714449174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)