FastQCFastQC Report
Sat 31 Dec 2022
SRR5846108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43162
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGC5691.318289235901951No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCTCGTATGCCGTCT5101.1815949214586905No Hit
TATATGGGGATCCTGGCTACAAGGTGAAGCAGCGCCTTGGCGGGGGCCGTGG3390.7854130948519531No Hit
GCAGTTGGGGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACG3070.7112738056623882No Hit
ACTATAGGGAGGAGGAAGCACTTGGTATCTGGTATGTGATCGCTGTAGAGAA2900.6718873082804319No Hit
CTCGCATCGGGATATTTAAGTACAGTTTTCATGAGGAAGTTTTAAAAGACCA2690.6232333997497799No Hit
TATAATGGGCAAGCAAAAACCAGGCTCCATCTACTCTTTGAAGCTTCTGCCC2660.6162828413882582No Hit
TCCTATGGGGTGGCAGTGAAGATGGATCCCTCCATGTCTTCGTGCTTGCTGT2650.6139659886010843No Hit
TATCTAGGGAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCCTTATC2430.5629952272832585No Hit
GGTACTGGGGTTTTATGAAACCATCATAACTTGACCCAAACCCCAAGAAAGA2420.5606783744960845No Hit
GGTCTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2410.5583615217089106No Hit
CAAAAGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGG2300.5328761410499977No Hit
TTCACTGGGTCCCCAAGATTGAGGCTATTGAGACATGGCACTATCAACAGGT2280.5282424354756499No Hit
GCTCCTGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA2250.5212918771141282No Hit
GCTTGCGGGAGGTGTTTGGTTATTGCTTTCAGAGTCAGACATTGTTTAGGTT2120.49117279088086746No Hit
GAAACCGGGACTGTGTGCCAAGCGCTTTTACGTGGCTTCTCCCTCAGCCAGC2050.47495482137065015No Hit
TCCTGTAGGGGGGTTAGATCGTCAGGTGAGGGAGGAAGGGATAGCCAGCGCG2010.4656874102219545No Hit
AATGTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2000.46337055743478056No Hit
GAACCTGGGTGACAGAATAAGACCCTGTCTCAATAAATAAATAAAATTTCTA1880.4355683239886938No Hit
GCTAGCGGGCGAAATCTACAATGTAATATGAGTGCATTGTATGGGTTTGAAA1840.42630091283999816No Hit
TGCATGGGGACTCGAGAATGAAGAGAATGTTTTCTATAGGGAACTGCTAGAG1830.42398406005282424No Hit
TCACTGGGTGAGGCTGATGCTACGCTCAGGCACACAGATTTTGGCACCAGCT1830.42398406005282424No Hit
AAGCTTGGGAAGGCTAGGATTGAACTCCATATGCAGAGGGTTTCTATTCACA1810.41935035447847646No Hit
AAACAAGGGAAGTTCAAGGATCCCATATAGTATAAGAATATAATTTCACTTG1730.40081553218108523No Hit
TACTCCGGGATCCCTAGAACGTTGAGGGCACGAGTCGGGTCCTGAGACCAGG1720.39849867939391137No Hit
GCTAGCAGGCGACCAGGTGAACGGAGGCAAGGTGGTGAGGAAGCACTCAGGG1650.382280709883694No Hit
TGGCATGGGGTGTTACTGGAAAAATTAAGTTGTTGAAAGTAAGTTGTTTTCC1620.37533015152217225No Hit
ATGGCAGGGAGGTAAAGTCTACTTTTATTTTGTCAAGCTTAATAATTTGTGT1580.36606274037347664No Hit
CGTAATGGGAGACTTAAAGATTTGAGAAATTATTTGTGTCATTTACTAGACG1540.3567953292247811No Hit
AAACAAGGGGTCTTAAATTACTTTTGAATTTCTAATCTGTGAAGGCAGTAAA1540.3567953292247811No Hit
TAACGAGGGAGCCTCTTTGATGGATTTGCAGATGGGTTAGGAGTGGCTGAAG1520.35216162365043324No Hit
GTATGAGGGATGGTGGGGCAATGGTTATTTGGTTATTTTACTCAATTGGTTA1510.3498447708632594No Hit
TTGACCGGGATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC1370.3174088318428247No Hit
GCTTATGGGTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAACAA1350.3127751262684769No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGT1340.310458273481303No Hit
CCTTCTGGGTATAACAAGTGTTGGAAGTTTGAGCATTGCAATTTCAACGACG1310.30350771511978125No Hit
GATTATGGGATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC1300.3011908623326074No Hit
GTTCTAGGGTCTGCCTTCTGAGTTGGCTCATCCTGGGCAGTCACAGACTGTC1290.29887400954543347No Hit
GTAACTACCCAGCCAGCAAGTATATAGCACAGAACACTGTGTTACTTTACAA1290.29887400954543347No Hit
TCCTAGGGGAGTGACATGCAGTTATATATTTTGTTTTTAAAAGGGGGGGAGC1260.2919234511839118No Hit
GCATGCGGGGCTCGGACCTAGGTCGCGGCGACATGGCCAAACGTACCAAGAA1250.28960659839673786No Hit
AAGCGCGGCAGCTATCTCAGATTTGAGCTATGTGAACTTTTAGTCAAGATGA1170.2710717760993467No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTA1140.26412121773782493No Hit
TGATACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1120.2594875121634771No Hit
TAAGAAGGGGTGTTACTGGAAAAATTAAGTTGTTGAAAGTAAGTTGTTTTCC1120.2594875121634771No Hit
CTTTAGTAACACGGACAAGGCTCCTCGCCAAGGAACTCGTGGCAGAAGAGGG1030.23863583707891203No Hit
ACATCGTGGGTAACACCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCC1000.23168527871739028No Hit
ATATCTGGGTGCAGAAGAGTTTGTGGGTCTAGTGTTCAGTGAAAATGAAAAC990.2293684259302164No Hit
GACATAGGGCTTCATCCTTATTACCATGTCTGAGAACCCCTATTAACTGGTA980.22705157314304247No Hit
GTGCGAGGGGCTACCCGTCCAGCCTGCCCAGACCCTCTCAGAGGAACGGGGT970.22473472035586858No Hit
CTAAGAGGGAACACATTTTGTATAATTGTATCGTATAGCTGTATTGAATCAT940.2177841619943469No Hit
TCATTGGGTGTTGTGAAAAAGGAAGAGAATCATTAAAGAAAAAGAAAAAAGA890.20619989805847738No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCCTGTCTCTTATAC870.20156619248412957No Hit
GTTGGCGGGACTGAATGAATTTTGAAGCTTAATGTTTTTAAAGGCATAGTTT850.19693248690978174No Hit
GCTGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA830.19229878133543396No Hit
TCTACAGGGATTTAAAGCTATTTTATTTTTCTACGCCATCTGGCTAGTAGAT810.18766507576108613No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTAGCGGTATCCTGTCTCT800.18534822297391224No Hit
ACGAAGGGTAGAAGAACAGACCTAACTAGCGAATGTATGAATGAAAATGCAT680.15754598952782542No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC650.1505954311663037No Hit
CGTTGGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTA590.13669431444326027No Hit
TAGCTGGGAGAAAAGAAGAGGGAACATTTATTTAAAGGATGGAAAGACTGGG590.13669431444326027No Hit
AGCCACGGTGCTATTCTGAGAGCCATTTTAAACTGCACTGCTCCAACCACCC580.13437746165608636No Hit
GCCACTGGGGTCTATTGGTTATTGATCTTGCCATCTTTATTTAAAACCATGT540.12511005050739077No Hit
CAAATGGGTTACCTCCAAATATGAAAATCTATAACAACCTATGGTTGAAGGA510.11815949214586906No Hit
TTCGGCGGGCTTTACTCTTATGGTATTTAGTCAAAATACTGTTTTCCAAAGT510.11815949214586906No Hit
TAACAGGGGGACGCGAAAATGACATGTCTCCAAGCAACAATGTGGTTCCTAT450.10425837542282564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)