FastQCFastQC Report
Sat 31 Dec 2022
SRR5846101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179842
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC11300.6283293112843497No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT9900.5504832019216868No Hit
CGTTAGGGGTCTTCGAGCGGGAGGCATGAAGGAGAGGCTGATGCAGGTAAAG4700.26134051000322506No Hit
TAGAGATGGGCCTATGGAAACTAATCAGAAAAGCTAGAGCAGTTTTGAGAGG3540.19683944795987587No Hit
TCCGGTGGGGAATTGTGATAAACCATTATTTTCATCTATTAGTATTTATTCA3210.17849000789581965No Hit
ATTAAGGGAGAAATGACAGTGACTTAAACCAGTAGTCTGAGATGGAGAGAAA3130.1740416587893818No Hit
GGCACTGGGGAATGACATGTGAGAAAACTCTTGTTAGACATGATGTTTCCTA2930.1629207860232871No Hit
GTTTTCGGGAGAGAATAAGAATTTTCAAACTTAGGGAACATTGCCTTACATA2380.13233838591652675No Hit
ACGACTGGGGGGGCAGGTGGTAGGTGGAGGGCAGCGACGTTGTCCTCAGGTG2360.13122629863991728No Hit
GGTTCAGGGTATTTCCATTTTACAGATGAGGAAACTGAGGAATAGAGGGGTT2210.12288564406534624No Hit
CATCATGGGGAGGCTGTACCCACCAGCATGGGGCACCTTTCGCCTGCTGGAA2180.12121751315043204No Hit
GAACTAGGGAGCAAAAGGTGATGGAGAAGGATTTGAGGTTGCCTTCTATTTA2110.1173252076822989No Hit
GCTTCAGGGACTGATTTTGTATCTTACAATAATTTTCTCATTTGATTTTTGC2090.11621312040568944No Hit
TAAACTGGGAAATCACAAGCAGACACCTACATATGATATATGAAGGAAACTT2090.11621312040568944No Hit
TAAGCACGGGGTCTGTTACATAGTAAGCGCTCAACAAATGTTCTGTGAACTA2090.11621312040568944No Hit
AAAGCCGGGACCTTTAAAAGAATCACAAAGATATAAAAATCCTTTACAAATA2070.11510103312907997No Hit
TTCGAAGGGAATAGACAAAGACTTCAAAGTAGCCATTATATATGTTCAAATA2070.11510103312907997No Hit
CATGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2040.11343290221416577No Hit
GAAGCTCGGGGATGTTTGGCTGGGCCATGCAATGGCGCGAATACCCTGATAG2040.11343290221416577No Hit
AAAGCTGGGACTAAGTATTATGTGTGGTTATTATAGATTTTCACAAAGATAT1990.1106526840226421No Hit
GCAAGCGGGTATTCTAATAAGAAAAGTTAAGGTGTACAACTCTCCTAACTGT1970.10954059674603263No Hit
GTAATTGGGGGAGCTGGTCATCGACAGGTGAGGGCCTTGACCAAGCCTGGTC1920.10676037855450896No Hit
GCAAAAGGGCTCACACTGGGAAAAAATATGAAATTACATATCTGACAAATGA1910.10620433491620423No Hit
TTGTGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1900.10564829127789949No Hit
ACCTTGGGGTCATATGCCTGTTGCCAATCACTTTTAGGGGAATGGGATATGC1890.10509224763959475No Hit
AAACTCGGGACTACTAAGACCACAAATACAACCTGATAAAAAGATTGTCCCA1890.10509224763959475No Hit
GCATCCGGGGCCCCAACACAGCCCTACTCGTTACCCAAGGCTTACAAAATCC1860.10342411672468056No Hit
AGTGCAGGGACTCCTCCCATAATGAATATTATATTAAATCCTTTCAGAAATA1850.10286807308637581No Hit
CTGAATGGGCAAGTATTGGCAAGGACACGGATAAATTGGAACCTTTGTGCAT1850.10286807308637581No Hit
ATCCAAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1850.10286807308637581No Hit
ACTGCAGGGACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTT1820.10119994217146161No Hit
GCTACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1820.10119994217146161No Hit
GGAGCTGGGAGAGAAACGAGGCATGGGAAGAATGGGGTGGAAGGATGCCAGA1810.10064389853315689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)