FastQCFastQC Report
Sat 31 Dec 2022
SRR5846099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179798
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC10980.6106853246420983No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT9880.5495055562353307No Hit
CGTTAGGGGTCTTCGAGCGGGAGGCATGAAGGAGAGGCTGATGCAGGTAAAG5030.27975839553276455No Hit
ATTAAGGGAGAAATGACAGTGACTTAAACCAGTAGTCTGAGATGGAGAGAAA3400.18910110234819075No Hit
TCCGGTGGGGAATTGTGATAAACCATTATTTTCATCTATTAGTATTTATTCA3040.16907863268779408No Hit
TAGAGATGGGCCTATGGAAACTAATCAGAAAAGCTAGAGCAGTTTTGAGAGG2970.1651853746982725No Hit
GGCACTGGGGAATGACATGTGAGAAAACTCTTGTTAGACATGATGTTTCCTA2720.1512808818785526No Hit
GTTTTCGGGAGAGAATAAGAATTTTCAAACTTAGGGAACATTGCCTTACATA2600.14460672532508706No Hit
GGTTCAGGGTATTTCCATTTTACAGATGAGGAAACTGAGGAATAGAGGGGTT2240.12458425566469038No Hit
GGAGCTGGGAGAGAAACGAGGCATGGGAAGAATGGGGTGGAAGGATGCCAGA2210.12291571652632398No Hit
GTAATTGGGGGAGCTGGTCATCGACAGGTGAGGGCCTTGACCAAGCCTGGTC2160.12013481796238001No Hit
TAAGCACGGGGTCTGTTACATAGTAAGCGCTCAACAAATGTTCTGTGAACTA2130.11846627882401362No Hit
ACCTTGGGGTCATATGCCTGTTGCCAATCACTTTTAGGGGAATGGGATATGC2080.11568538026006964No Hit
ACGACTGGGGGGGCAGGTGGTAGGTGGAGGGCAGCGACGTTGTCCTCAGGTG2030.11290448169612566No Hit
TGGCCAGGGCAGGATGAGGGACGGCTTGAGCAAATTTTACCTTTCACATGGT1990.11067976284497047No Hit
CTGAATGGGCAAGTATTGGCAAGGACACGGATAAATTGGAACCTTTGTGCAT1980.11012358313218167No Hit
GCTTCAGGGACTGATTTTGTATCTTACAATAATTTTCTCATTTGATTTTTGC1970.10956740341939288No Hit
GAACTAGGGAGCAAAAGGTGATGGAGAAGGATTTGAGGTTGCCTTCTATTTA1970.10956740341939288No Hit
TAAACTGGGAAATCACAAGCAGACACCTACATATGATATATGAAGGAAACTT1950.10845504399381528No Hit
ACAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1950.10845504399381528No Hit
TAAGATGGGGGGGAAACTGATTTGGAATCCTAGCTGTACCCCATAGTGGTTG1940.10789886428102648No Hit
ACCGTTGGGTGGCTTTAAAGTGCCTTTTAGTCCAAATGGACCTAACAGACAT1900.10567414542987129No Hit
GAAGCTCGGGGATGTTTGGCTGGGCCATGCAATGGCGCGAATACCCTGATAG1900.10567414542987129No Hit
TCAGCCGGGAGTGCTGAAATAATTGGGAGATCATATGGAACAAGGAGAAATT1890.10511796571708251No Hit
AAACTCGGGACTACTAAGACCACAAATACAACCTGATAAAAAGATTGTCCCA1870.10400560629150492No Hit
GCTACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1850.10289324686592732No Hit
GTAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1830.10178088744034973No Hit
TCAGGCGGGATTTATTAATTCAACAAATTTCTTGAGTATGAATTATATGCCA1820.10122470772756093No Hit
TTTACAGGGGAAGAATGTAGTAAGAAGAGGGATACTAGAGAAACTCAAAAAA1820.10122470772756093No Hit
TTGTGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1820.10122470772756093No Hit
GTTTCCGGGAGTGGGTGGCTGCTGTGGGCAAGGACCGGCCCTTCATGGGGGG1820.10122470772756093No Hit
GCAAAAGGGCTCACACTGGGAAAAAATATGAAATTACATATCTGACAAATGA1820.10122470772756093No Hit
GCAAGCGGGTATTCTAATAAGAAAAGTTAAGGTGTACAACTCTCCTAACTGT1800.10011234830198333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)