FastQCFastQC Report
Sat 31 Dec 2022
SRR5846089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53750
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT3810.7088372093023255No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC3550.6604651162790698No Hit
CAACATGGGATACCTGACTGTTTCTCACAAAATCTCAACGATGTAAGCAAAG1420.2641860465116279No Hit
GAAAAAGGGATGGAGGATAACATAAACCGATACATGTCAAACTGTTCAGAGA1080.20093023255813952No Hit
TTGGCCAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.19906976744186047No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCTGTC970.18046511627906975No Hit
AATGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.17116279069767443No Hit
GGCGACGGGATCGATATGTCTTCCTAAAACCATGCATGTGGCCAGGCATGGT910.16930232558139535No Hit
TGCGCAGGGTTCTCAGGGATGAAATTCTTTTTCAAAGTTTTGAAGTTCCTTG910.16930232558139535No Hit
TACCATGGGATCTAAGGTGATGGGTTGATTTGTGCAGCAAACCACCATGGCA900.16744186046511628No Hit
TAACGAGGGTATGAAAGAAGCTTTCAGTCCTTCGCTCCCCAAGGCTGAGGGA890.1655813953488372No Hit
TCATCAGGGCAGCAATAAATATAGCCAGCATTCAAAAAACAAATTATTCCTA870.16186046511627905No Hit
GATGTTGGGGGGGATCCAAACAAAACCCGTCTATTGAGATTGGTCAATGCAT870.16186046511627905No Hit
GCGCACGGGATAATCACTTGTTCCTTAAGTAGGGACCCGTATGAATGGCTCC860.16No Hit
CTTTATGGGATGGAGGTGAGGTATTAAATGGTCTTCTGCTTAAATGGAAAAA850.15813953488372093No Hit
TAAGCTGGGAGAAGCTTTCATTCCACAAACTATAGAACAGATGAGCAAGAGC840.15627906976744185No Hit
GTACTTGGGATCTCACTTTGCTTGTATGTATTTTTTCTTCTCTAAAATTTTT840.15627906976744185No Hit
ACAACAGGGCTTGTCGAGGGATTAACAAAAATTCCCAAGGCAAAATTAATTT840.15627906976744185No Hit
AAAGCAGGGAGTGCACAGAGAGCTGTCGTATCAGCCTTCAGAAGAGCATTTA820.15255813953488373No Hit
TCGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.15069767441860465No Hit
CAGCTAGGGTATTCTGAAAAGACTGTGGAGGAAGAAAACCCTTTACCCTGTT810.15069767441860465No Hit
AGATCGGGGCATCAGGCAGTACTGGTAGAGTGGTAATATAAGAGAGATTTAG810.15069767441860465No Hit
GCTCCGGGGCGTGATTGAATTACTAGGTAGTAAAATGTGCACTGGACTGAAA780.14511627906976743No Hit
CAAGTAGGGGATGGTCCCACTGACTTCCCAGCTAATTTGCTGCCTTTGATAG770.14325581395348835No Hit
AGTCAAGGGATTTTATTACATGAGGTGGTGGCATTGTATATTGACAAAATAT750.13953488372093023No Hit
TTACCTGGGATCTGTCTGTTTATGTAAGGGAATGTTTGATCATTGTTTGTTT750.13953488372093023No Hit
GTAGATGGGGGACTCCCATATTCACTGCAACATTACTCACAATAGCCAAGAT750.13953488372093023No Hit
TATAGTGGGAGGTTGCAAGGATAATAATTTTAGTAATTTATTTCCTACCGCC740.13767441860465116No Hit
TTTGTTGGGGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTAT740.13767441860465116No Hit
CATACCGGGATGGATTTTGCTTCTTTCTAAAGTTTAGCTTACAAATGGCTAT740.13767441860465116No Hit
TGCAAAGGGATTGTAATAAAGCTTTTACTACTATGAAGGAAGACCATGAATC730.13581395348837208No Hit
TGACCAGGGCAGCAGATCCCACCCCCATGGAGCCCAGCAAGCTAAGATCCCC730.13581395348837208No Hit
CAGTATGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT730.13581395348837208No Hit
GAAACTGGGCGATCTAGAATAGGGATAAGTGAAAATGGTGATCACCTAAGCA730.13581395348837208No Hit
TACATGGGGATGAAAGACTGCAGTGTTCCATTTACAGGCATTGGTTGGTGGT690.12837209302325583No Hit
ACCTGGGAACTTAAAGGGAAACTTTCACAATGTCCGGAGCCCTTGATGTCCT690.12837209302325583No Hit
TTAGCAGGGAGCAGGAGTCCAGAGGGAAGTACAGTTAATTTTCATTAATACC680.12651162790697673No Hit
CTAACCGGGTGGGGGCTGGGATTTCAACATATGAATTTTGTGGGACACAATT680.12651162790697673No Hit
GTCACAGGGAAAAGAGAGGTACTAACAACCTATGAAAAGACATGGAAGAAAC670.12465116279069767No Hit
AACATCGGGTTGTCTGATTTTGTCCGTTTCTTAAAATTATGATTAGTGAGTG670.12465116279069767No Hit
ATGTAAGGGAAGAGGATGCTGGGGAATTGTGCTGCTTGTCCTGTTGGCTGGT670.12465116279069767No Hit
GCTTTGGGAATGTTCCGATATTTCTTCATCTTATGTTTTATGTTACAAAACA670.12465116279069767No Hit
TAGTAGGGGATATAGGCATCTTCCATTGACATTAATAAAACTTAGAAACAGT660.12279069767441861No Hit
GACTATGGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.12279069767441861No Hit
ATAAAAGGGTGCACCACCACATCCAAAAATAATTTTTATTGCTAAAGTTATG660.12279069767441861No Hit
TACATTGGGGTGGGACAGGCTCTTCTTTCCCAAAATTAGGGAGCTCTTGACA660.12279069767441861No Hit
GCTGCAGGGGAATGCTAAAACACAGCTGGAATCTAAGAAGGGAAGAGGGCTT660.12279069767441861No Hit
CCACAAGCGGGTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG660.12279069767441861No Hit
ATACTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA650.12093023255813953No Hit
TTAACCGGGTAGATGTGATTAATTATCCAGTTCCCTACACTGGGAAAGAAAT650.12093023255813953No Hit
AACTATGGGATATATGATTAAGTGTCTTAGAAATCTTTCTTTATATTAACTA650.12093023255813953No Hit
GACCTGGGGAAGCAGCGCCTGGCAAAGTGGCGGCGCTCAGAAGGCCTGCCCT650.12093023255813953No Hit
TTTCATGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA650.12093023255813953No Hit
TACACTGGGGCTTTGTGACAATTTACCTTTTAAGTTACCAATGACACTTTTG640.11906976744186046No Hit
TAAACTGGGCCCCTGGGATTTCAACAGCCATCTAACTATTCATCCAATTATT640.11906976744186046No Hit
CTGTCTGGGAGAGTGCCCACTGAGTCAGGCTATGGAGGAAACCCTGGGGATA640.11906976744186046No Hit
TTAATAGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11906976744186046No Hit
GAATGAGGGGTGTAAAACAGGTGAGTGGAGAAGACGTCCTGGTTTCTCCAAA640.11906976744186046No Hit
ATCAAGGGGTACAGCCTGCCATCTCTTCTTATAAATAATGTTATATTGAAAC630.11720930232558138No Hit
ACAACAGGGATGTATTATGAAGGTACTGGTTTCATCCTACTTCTTAACCATG630.11720930232558138No Hit
AACGACGGGGATCTAATCGGGGGATGTGCAAGCACTGTTCTAAGCCCTAGGC630.11720930232558138No Hit
GCTCAAGGGAGATTTTGTGAATATGAACATGCTGTTATAGGATCTACAGATG620.11534883720930234No Hit
CAGCACGGGGTTTTACGAAAAGTTGTCAATGTTATTTAAAAAAAGAAAAATC620.11534883720930234No Hit
TTATCAGGGGTATTTTATGAGGATTTTATAAATGTAAATTAAGAAGACAATA620.11534883720930234No Hit
CTTGTTGGGATGTAGTAGATGCTTAAAGTGGAAAATAGTGTTATGATGACTT620.11534883720930234No Hit
CTTCTAGGGGATTTATAGACACCACATCTGGGGGGCTTTCAGGTTACAGCCT620.11534883720930234No Hit
TAGCTAGGGACTGTAGGTCATGTCCTGAGGGAAGAGGGCCCCAGCCTTTCTA620.11534883720930234No Hit
CGCAAGGGGCCGATCTTTGGCCTGGGTGCAGGACCTGCACATGTTACAGGTT620.11534883720930234No Hit
GCTACTGGGGCAGAGACTAAGCAGGCTATTCTCATCTCTTTGAGTTTCCTGA620.11534883720930234No Hit
CATTTAGGGAAGCATGTTTAATTGAAGAAAGTATTAATATCTCTTTAGATAA610.11348837209302326No Hit
CAAATTGGGACCCTGATAGAACTTTCTGGCAAAGGTGTATACATTTGTCACA610.11348837209302326No Hit
TAGTTGGGGAATAGACATGATGCTTTATAATGAAGAAGTTTGTAGCAGAAAC610.11348837209302326No Hit
TATGCAGGGAGTGTTGGGTGCTATGAGTAGGTATGGATCTCTGTTGATTGAC610.11348837209302326No Hit
GCGCTGGGGCTACTTTGCAGCTCTTTGCTTTTGTTCTTTGCCACTTTCTTCT600.11162790697674418No Hit
GTCCAAGGGAATTATATTCTTACTGTGGTTCACAAGAAGGTATGTAAGAGGT600.11162790697674418No Hit
TCTCATGGGACCCTTTACATTTTTGTATCATAATAGATTTTAAAAATCTAAT600.11162790697674418No Hit
ATATGCGGGATTTCTCCTCATTTCTTCCTTATGTTTAAACACGCCCCCATCT600.11162790697674418No Hit
TTGAGTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA590.10976744186046512No Hit
TTGTCGGAGGGAGGAGTTCGAAACCAGCTTTGCCAACATGGTGAAACCCCAT590.10976744186046512No Hit
ATGCATGGGGTAAGACCGAGGACCAGCGCAACGAGGAGAAGGCGCAGCGTGA590.10976744186046512No Hit
GTTGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC590.10976744186046512No Hit
TAATGTGGGAACCAGTTACAGACTTTGTTTCTTCTCCAGTCCTGCTGCTTCA590.10976744186046512No Hit
GTAGAGGGGAAAGTACCTAATGCTGCACTTGGATGTTTCAACTAAGGAAATG590.10976744186046512No Hit
TCGCATCGGGATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTA590.10976744186046512No Hit
GCTTAAGGGTAGCTGGGCATGGTGGCGCACTCCTGTGATCTCAGCTACTCGG590.10976744186046512No Hit
GACTAAGGGCCCTCCTCTACCTCAAATTCTCATGGAGCCCCGTAGCCCCAGA590.10976744186046512No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACT590.10976744186046512No Hit
AAAGTTGGGAATAAGAAACAGTTTAACAGAAAAATGAAATTTAAGCACAAAA590.10976744186046512No Hit
GGTGTTGGGAATCTGGGCTTTGTAGCATATGCCTGTGGTCCCAGCTACTGAG580.10790697674418605No Hit
CAAGAAGGGACAGAATGGGAGAAAATATTTCCAAGCCATGTATCTGATAAAT580.10790697674418605No Hit
AAAAGGTGGGCTGTGTAAACTCCAAAGCTAAAACTGCTTCTGTCGAAATGGT580.10790697674418605No Hit
AAAGCAGGGATTAGATCTTCATGCCTTTGATAGTCTCTCTGGAATAAGTTGT580.10790697674418605No Hit
AAACTAGGGTTAATACTAAAAGCTCTGCATAAGCAGTGGTTCAGAACCTGTC580.10790697674418605No Hit
TATACAGGCTCCCTTAGCTGGCTCTAGGTTTTATTGTTTTTGTGTCTTTCCT580.10790697674418605No Hit
GCACTCGGGTCCTTTCAAAAACAAAAGTTCACATGATTGCTTTAACAATATA580.10790697674418605No Hit
TTGGTTGGGCTAAAGGGGAATCATGATTATAAGTCCTTCAGCCCTTGGACTC570.10604651162790697No Hit
CTGAGCGGGCGATCTGTGTCGGGAACTCTAGCTTTGACAAAGATGATAACCA570.10604651162790697No Hit
ACTATGGGGCATGCCCAGATGGAGCTAAGCAAAATAAGTAGATTTAACTGTG570.10604651162790697No Hit
TGAACTAGGGTTCACTAAATTCCTAAGAAAAATTTAGCACAGCCGATGCTGT570.10604651162790697No Hit
TGTACCGGGCTCCAAGATACTCTTCTGAGTTGTAGAATTTTAACATTAATAA570.10604651162790697No Hit
GCACCCTTAAAAGTGATTTTGTTGCCGCTGCATAGATTCTGTGTAACTTTTT570.10604651162790697No Hit
GCAAACGGGATAATACATTCACGACAAAGTGTGTGTCACAACAGTGGGAAAA560.1041860465116279No Hit
GCGCTTGGGAGGAGATCTGCACATCCCACACCGGTGCAACAGTTTGCCAGCT560.1041860465116279No Hit
TTATTAGGGAAGTGGAGCTGGGGGAGCTGCTTCTGTCACTGAATTATCTCCC560.1041860465116279No Hit
GCTTGAGGGCATATAAAGCTGAATACAAATTGGTTTGGGGGGAGATCCTTTC560.1041860465116279No Hit
CAGTAAGGGCTCCCAGGCACTTGGATCCCCATTAGAGAGTTAGGATATAATC560.1041860465116279No Hit
AATCTGGGGAGCTTACGAGGAAATATTTCAGTAAAAGCAGTTAAAAAAGAAG560.1041860465116279No Hit
ACACATGGGACCACACCTGGCCTCCAAGTGTTCCCTGTTACCATCTTTATGT560.1041860465116279No Hit
CAGCTTGGGGAGCACTACTCACAGATCCACACCTGACCCTGTTGGGTCGAGT550.10232558139534884No Hit
TTCATAGGGCTTCACTGTAAACTATTAAATTCACCTTGATTATTTTATTAAT550.10232558139534884No Hit
ATTTCTGGGTATGAGCTTCTTTCCATTTTCCTCTTCTGTTCTTCTAATTTAA550.10232558139534884No Hit
ATAACAGGGACTTTTTTATACAAACTATTTTCTTGAAAACTTAGTAGTTTTG550.10232558139534884No Hit
AACGGTGGGCAAGCACTCTGAAGAAAAAGAAATTCCAGAACTTAAATTCCCA550.10232558139534884No Hit
TGGTTTGGGAGATGTTAAGAGATTGAGGCCCTGGGCTGAGGGTATATAATGT550.10232558139534884No Hit
ATGCTAGCGGGATTTTAAAAAACGTATTTAACAGAAGAGAGCAAATAAAGAT550.10232558139534884No Hit
ATTACAGGGAAGCAATGCTGCCCGTGACACAAACAGACAGATTACTATTTCT550.10232558139534884No Hit
CATCATGGAATGAAAATTACAGATAAACAAATTAAAAAATCTTTCCAGTTAC550.10232558139534884No Hit
AGCTTAGGGTAGTACTAAATCATAAAGAATAAAATGCTTTTTTTTTTTTTTT550.10232558139534884No Hit
GTATGAGGGTTACATAAATTAAAAAACCATTGAGCTCTTTCATAAAAGTTTC550.10232558139534884No Hit
TCTCTAGGGATGTATGTCTGGTTTTCATGTGGATAACATTTGGCAATCAAAT550.10232558139534884No Hit
GTCAAAGGGAACAGCAGAGGAGGAAATTTCTGTAATGGGCCATAGCAGGAAC540.10046511627906976No Hit
CAAAGCGGGACCTGGCCGTGGGGAACTACCGGCTCAAGGAATACGAGAAGGC540.10046511627906976No Hit
AAAGTTGGGAGCCTTGACCTGTACTTTACTAAATGTACAAAAATCAATTCAC540.10046511627906976No Hit
ATCGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC540.10046511627906976No Hit
CGCTATGGGTCAACAGAATAACCATGTGCATGGACAGCCATATACAGGCCCA540.10046511627906976No Hit
TAGCGAGGGCCACCCTCTGAGACTCCATTGGTGTCACACAAGGTGTCTTCTT540.10046511627906976No Hit
AGAACAGGGTGGAGAGTACCTTGTTATATATACAGATACTTTCATGGTCATT540.10046511627906976No Hit
CAACCTGGGCACTCAAGGTTAATGATTTTTGTTTTTTATTTATGTTTGTAGA540.10046511627906976No Hit
GCGCGGGGACATTCTACTATTGAATTATGTACAACATTTTCATTTTTTATGC540.10046511627906976No Hit
AGTAGTGGGCGAGTAGCTGGGATTGCAGGCGTGCACCATCACACCTGGCTAA540.10046511627906976No Hit
CTGCTAGGGGAGTTCACTTTGATGTTAAATCTATTGCTACTTCAGTTGTAAA540.10046511627906976No Hit
GTTTTTGGGAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGC540.10046511627906976No Hit
TGACTAGGGTAAAGAGACCCAGGCTCAGTGGCTTCTGTGCCTTCCCAGAGGT540.10046511627906976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)