FastQCFastQC Report
Sat 31 Dec 2022
SRR5846087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55427
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT4340.7830118895123315No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC3470.6260486766377398No Hit
CAACATGGGATACCTGACTGTTTCTCACAAAATCTCAACGATGTAAGCAAAG1560.28145127825788874No Hit
GAAAAAGGGATGGAGGATAACATAAACCGATACATGTCAAACTGTTCAGAGA1120.2020675843902791No Hit
AATGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.1768091363414942No Hit
CATACCGGGATGGATTTTGCTTCTTTCTAAAGTTTAGCTTACAAATGGCTAT960.17320078662023924No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCTGTC950.17139661175961174No Hit
GATGTTGGGGGGGATCCAAACAAAACCCGTCTATTGAGATTGGTCAATGCAT930.16778826203835676No Hit
TGCGCAGGGTTCTCAGGGATGAAATTCTTTTTCAAAGTTTTGAAGTTCCTTG930.16778826203835676No Hit
ACAACAGGGCTTGTCGAGGGATTAACAAAAATTCCCAAGGCAAAATTAATTT920.16598408717772925No Hit
ATACTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA910.16417991231710177No Hit
AGTCAAGGGATTTTATTACATGAGGTGGTGGCATTGTATATTGACAAAATAT910.16417991231710177No Hit
ATTACAGGGAAGCAATGCTGCCCGTGACACAAACAGACAGATTACTATTTCT910.16417991231710177No Hit
GTACTTGGGATCTCACTTTGCTTGTATGTATTTTTTCTTCTCTAAAATTTTT900.1623757374564743No Hit
TTGGCCAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.1623757374564743No Hit
GCTCCGGGGCGTGATTGAATTACTAGGTAGTAAAATGTGCACTGGACTGAAA890.1605715625958468No Hit
TAAGCTGGGAGAAGCTTTCATTCCACAAACTATAGAACAGATGAGCAAGAGC870.1569632128745918No Hit
TAACGAGGGTATGAAAGAAGCTTTCAGTCCTTCGCTCCCCAAGGCTGAGGGA850.15335486315333682No Hit
CAAGTAGGGGATGGTCCCACTGACTTCCCAGCTAATTTGCTGCCTTTGATAG850.15335486315333682No Hit
TATAGTGGGAGGTTGCAAGGATAATAATTTTAGTAATTTATTTCCTACCGCC840.15155068829270932No Hit
GTTGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.15155068829270932No Hit
TGTACCGGGCTCCAAGATACTCTTCTGAGTTGTAGAATTTTAACATTAATAA840.15155068829270932No Hit
CAGTATGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT810.14613816371082686No Hit
AAACTAGGGTTAATACTAAAAGCTCTGCATAAGCAGTGGTTCAGAACCTGTC800.14433398885019935No Hit
AGAAAGGGAAAGAATTAATGTTAAAATGTTTATTATAGCATTATTTTTATGT800.14433398885019935No Hit
TACATTGGGGTGGGACAGGCTCTTCTTTCCCAAAATTAGGGAGCTCTTGACA790.14252981398957187No Hit
TCATCAGGGCAGCAATAAATATAGCCAGCATTCAAAAAACAAATTATTCCTA780.14072563912894437No Hit
GGTAATGGGTATTTACCCAAGAGCATTGAAAGCAGGGACTTGAATAGATATC780.14072563912894437No Hit
ATCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA770.1389214642683169No Hit
TACCATGGGATCTAAGGTGATGGGTTGATTTGTGCAGCAAACCACCATGGCA760.13711728940768939No Hit
GCTTTGGGAATGTTCCGATATTTCTTCATCTTATGTTTTATGTTACAAAACA760.13711728940768939No Hit
CTGTCTGGGAGAGTGCCCACTGAGTCAGGCTATGGAGGAAACCCTGGGGATA750.1353131145470619No Hit
GAAACTGGGCGATCTAGAATAGGGATAAGTGAAAATGGTGATCACCTAAGCA750.1353131145470619No Hit
AATACCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC740.1335089396864344No Hit
ATGTAAGGGAAGAGGATGCTGGGGAATTGTGCTGCTTGTCCTGTTGGCTGGT740.1335089396864344No Hit
ATCAAGGGGTACAGCCTGCCATCTCTTCTTATAAATAATGTTATATTGAAAC730.13170476482580692No Hit
ACCTGGGAACTTAAAGGGAAACTTTCACAATGTCCGGAGCCCTTGATGTCCT730.13170476482580692No Hit
ATTTCTGGGTATGAGCTTCTTTCCATTTTCCTCTTCTGTTCTTCTAATTTAA720.12990058996517942No Hit
ATAATTGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA710.12809641510455194No Hit
TTTAGGGGGCCAGTGATATTTTAGAGTTCGTATTGCTCCATATTCTCAGAAA700.12629224024392444No Hit
TGCAAAGGGATTGTAATAAAGCTTTTACTACTATGAAGGAAGACCATGAATC700.12629224024392444No Hit
CAAGAAGGGACAGAATGGGAGAAAATATTTCCAAGCCATGTATCTGATAAAT690.12448806538329694No Hit
GGCGACGGGATCGATATGTCTTCCTAAAACCATGCATGTGGCCAGGCATGGT690.12448806538329694No Hit
ATATGCGGGATTTCTCCTCATTTCTTCCTTATGTTTAAACACGCCCCCATCT690.12448806538329694No Hit
GTAGATGGGGGACTCCCATATTCACTGCAACATTACTCACAATAGCCAAGAT690.12448806538329694No Hit
TTAATAGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.12448806538329694No Hit
TTATGAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT690.12448806538329694No Hit
GAAGCAGGGACATGCACCTGCCTTAGAAGGTAGGATTTTCTTCCCAGGGACC680.12268389052266947No Hit
CATTTAGGGAAGCATGTTTAATTGAAGAAAGTATTAATATCTCTTTAGATAA680.12268389052266947No Hit
CAGCTAGGGTATTCTGAAAAGACTGTGGAGGAAGAAAACCCTTTACCCTGTT680.12268389052266947No Hit
GCGCACGGGATAATCACTTGTTCCTTAAGTAGGGACCCGTATGAATGGCTCC680.12268389052266947No Hit
CAGTAAGGGAGAAAATGTCGACATTACTCTGAAGGGACGCACAGTTATCGTG680.12268389052266947No Hit
TACCATGGGATTTATTTCAGATGGCGTTTTAAATAGATACAACCCTTTGGAA680.12268389052266947No Hit
ACAACAGGGATGTATTATGAAGGTACTGGTTTCATCCTACTTCTTAACCATG680.12268389052266947No Hit
TATGCAGGGAGTGTTGGGTGCTATGAGTAGGTATGGATCTCTGTTGATTGAC680.12268389052266947No Hit
CTTGTTGGGATGTAGTAGATGCTTAAAGTGGAAAATAGTGTTATGATGACTT680.12268389052266947No Hit
CATAGAGGGCTCTTTCATATTCATTTTCTTGCAAACTCTAAAAACCTTGACC680.12268389052266947No Hit
GAATGAGGGGTGTAAAACAGGTGAGTGGAGAAGACGTCCTGGTTTCTCCAAA680.12268389052266947No Hit
TTTCATGGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA680.12268389052266947No Hit
GCTACTGGGGCAGAGACTAAGCAGGCTATTCTCATCTCTTTGAGTTTCCTGA680.12268389052266947No Hit
ACCACTGGGGACCTCAAACGGGTGGCGATTTGATGGGTACCCCCAGGCTGGG670.12087971566204198No Hit
TCGTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC670.12087971566204198No Hit
TTAGCAGGGAGCAGGAGTCCAGAGGGAAGTACAGTTAATTTTCATTAATACC670.12087971566204198No Hit
CGCAGAGGGATGGAAAGCAAAGTTTCTCAGAATGGAGACATTCTCATTGAAG670.12087971566204198No Hit
TCTCTAGGGATGTATGTCTGGTTTTCATGTGGATAACATTTGGCAATCAAAT670.12087971566204198No Hit
GCGCTGGGGCTACTTTGCAGCTCTTTGCTTTTGTTCTTTGCCACTTTCTTCT660.11907554080141447No Hit
TGACCAGGGCAGCAGATCCCACCCCCATGGAGCCCAGCAAGCTAAGATCCCC660.11907554080141447No Hit
CAACATGGGGACGTGCCCGGAGCAGCTCCCTTCGCTGTGGAAGCGGCGGTGT660.11907554080141447No Hit
TAAACTGGGCCCCTGGGATTTCAACAGCCATCTAACTATTCATCCAATTATT660.11907554080141447No Hit
CTTTATGGGATGGAGGTGAGGTATTAAATGGTCTTCTGCTTAAATGGAAAAA660.11907554080141447No Hit
CGTTAAGGGAAGGCATGTGCCACCATGCCTGGCTTATTTTTGCGTTTTTGGT650.11727136594078698No Hit
GGGCATGGGAAAACTGAATACTTACAGATACTCGTGATTGTGTGGTTGCCTC650.11727136594078698No Hit
TGTACAGGGGGATGCTATTCTAATAGATATACTGAGAAATTTTTGTATCTTT650.11727136594078698No Hit
ATGCTAGCGGGATTTTAAAAAACGTATTTAACAGAAGAGAGCAAATAAAGAT650.11727136594078698No Hit
CTTCTAGGGGATTTATAGACACCACATCTGGGGGGCTTTCAGGTTACAGCCT650.11727136594078698No Hit
GCACCCGGGCTGAGACAGGGCACTTAATTCCATTTTACTGTACTGAATTAGT640.11546719108015949No Hit
CTGAGCGGGCGATCTGTGTCGGGAACTCTAGCTTTGACAAAGATGATAACCA640.11546719108015949No Hit
TATTAAGGGGGCCTACTCTTCTAAAAATTGTAGCTTATCGATTTTTCTCTGT640.11546719108015949No Hit
GGTGCTGGGAAGATATTTATTTCTGCCTGGATAATTGAGAGTTGACTAACTT640.11546719108015949No Hit
TGTTCAGGGGTCCTTGAACCACAAAGGACAGTTGAGTCCAGAATGATATCAG640.11546719108015949No Hit
AAGTCAGGGATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC630.113663016219532No Hit
ACTATGGGGCATGCCCAGATGGAGCTAAGCAAAATAAGTAGATTTAACTGTG630.113663016219532No Hit
GACTATGGGGCCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.113663016219532No Hit
TTAACCGGGTAGATGTGATTAATTATCCAGTTCCCTACACTGGGAAAGAAAT630.113663016219532No Hit
ACCAACGGGGCATATAACTTGGTGTGCATATGTGTACATATGCGAAGACATA630.113663016219532No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACT630.113663016219532No Hit
GTCACAGGGAAAAGAGAGGTACTAACAACCTATGAAAAGACATGGAAGAAAC620.11185884135890449No Hit
GAGCAGGGGCATCCTATGCTGTGTTCTTTGCAACCTACCTGACCCTGGCTTG620.11185884135890449No Hit
TTGTCGGAGGGAGGAGTTCGAAACCAGCTTTGCCAACATGGTGAAACCCCAT620.11185884135890449No Hit
AGATCGGGGCATCAGGCAGTACTGGTAGAGTGGTAATATAAGAGAGATTTAG620.11185884135890449No Hit
TACATGGGGATGAAAGACTGCAGTGTTCCATTTACAGGCATTGGTTGGTGGT620.11185884135890449No Hit
GTAGAGGGGAAAGTACCTAATGCTGCACTTGGATGTTTCAACTAAGGAAATG620.11185884135890449No Hit
ACCTTGGGGAGAGGGTCACTTGTTTGTATTGCAATAAACACTGGGACCAGTT620.11185884135890449No Hit
TGGTTTGGGAGATGTTAAGAGATTGAGGCCCTGGGCTGAGGGTATATAATGT620.11185884135890449No Hit
TAGTTGGGGAATAGACATGATGCTTTATAATGAAGAAGTTTGTAGCAGAAAC620.11185884135890449No Hit
TCGTTCGGGTGAAACCCAAGAGTTGCTTTGTTCCAGGGCATGTGATTGATAC620.11185884135890449No Hit
CTGCTAGGGGAGTTCACTTTGATGTTAAATCTATTGCTACTTCAGTTGTAAA620.11185884135890449No Hit
TTACCTGGGATCTGTCTGTTTATGTAAGGGAATGTTTGATCATTGTTTGTTT610.11005466649827703No Hit
TTCATAGGGCTTCACTGTAAACTATTAAATTCACCTTGATTATTTTATTAAT610.11005466649827703No Hit
ATAACAGGGACTTTTTTATACAAACTATTTTCTTGAAAACTTAGTAGTTTTG610.11005466649827703No Hit
TAAACGGGGACCTTTTTGACAGATGGGCAAATTGAGGCACAGAACAATTGAG610.11005466649827703No Hit
ATACCTGGGTACGAGGACCAGTGGCTGCAGCTGCTGCGGACGTATGTGGGCC610.11005466649827703No Hit
GACCTGGGGAAGCAGCGCCTGGCAAAGTGGCGGCGCTCAGAAGGCCTGCCCT610.11005466649827703No Hit
GCTTAAGGGTAGCTGGGCATGGTGGCGCACTCCTGTGATCTCAGCTACTCGG610.11005466649827703No Hit
GACTAAGGGCCCTCCTCTACCTCAAATTCTCATGGAGCCCCGTAGCCCCAGA610.11005466649827703No Hit
ACGAGTGGGAGAGAGGAAGAATAAATCCACAGCAAGTTAATTAGAAATACAG600.10825049163764952No Hit
CAGCTTGGGGAGCACTACTCACAGATCCACACCTGACCCTGTTGGGTCGAGT600.10825049163764952No Hit
TTTGTTGGGGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTAT600.10825049163764952No Hit
ATAAAAGGGTGCACCACCACATCCAAAAATAATTTTTATTGCTAAAGTTATG600.10825049163764952No Hit
AACGGTGGGCAAGCACTCTGAAGAAAAAGAAATTCCAGAACTTAAATTCCCA600.10825049163764952No Hit
AAGCCTGGGTTTCCTTTCCCTCTGCCCACCAAGTCCTCATATCTGCAGAGAA600.10825049163764952No Hit
CAGCACGGGGTTTTACGAAAAGTTGTCAATGTTATTTAAAAAAAGAAAAATC600.10825049163764952No Hit
CCACAAGCGGGTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG600.10825049163764952No Hit
AACATCGGGTTGTCTGATTTTGTCCGTTTCTTAAAATTATGATTAGTGAGTG590.10644631677702203No Hit
GTCAAAGGGAACAGCAGAGGAGGAAATTTCTGTAATGGGCCATAGCAGGAAC590.10644631677702203No Hit
GTAAGTGGGATACCCAAAGGACTATAAATCATGCTGCTATAAAGACACATGC590.10644631677702203No Hit
ACGCCTGGGCGATCTAACACAGGGATATAAATTGCTTCTTCATTTTAATTTG590.10644631677702203No Hit
AAAGCAGGGAGTGCACAGAGAGCTGTCGTATCAGCCTTCAGAAGAGCATTTA590.10644631677702203No Hit
TTATCAGGGGTATTTTATGAGGATTTTATAAATGTAAATTAAGAAGACAATA590.10644631677702203No Hit
GTTTTTGGGAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGC590.10644631677702203No Hit
GTCCAAGGGAATTATATTCTTACTGTGGTTCACAAGAAGGTATGTAAGAGGT580.10464214191639454No Hit
CGGCAGGGGCTCGCTGCAACCTCTGCCTCCAGGGTTCAAGTGGTTCTCATGC580.10464214191639454No Hit
GCCACTGGGGAATAGAATGGGAGGGATGTTTAGTATATCCAGTATGATTGGT580.10464214191639454No Hit
GCTCAAGGGAGATTTTGTGAATATGAACATGCTGTTATAGGATCTACAGATG580.10464214191639454No Hit
TAAATAGGGTTTGGGAAGCTGAGAAAGGAGGATCTCTTGAGCCCCGGAGTTT580.10464214191639454No Hit
ATTCTTGGGAAGATACATGAAATAAAAAGCAACAACTAAAGGGAGAAATGCA580.10464214191639454No Hit
AACGACGGGGATCTAATCGGGGGATGTGCAAGCACTGTTCTAAGCCCTAGGC580.10464214191639454No Hit
CAAATTGGGACCCTGATAGAACTTTCTGGCAAAGGTGTATACATTTGTCACA570.10283796705576705No Hit
GCGCTAGGGCGATCTGTACCTGGGTAAGTTGAAAATACCATAAGTGAAAAGT570.10283796705576705No Hit
TTGGTTGGGCTAAAGGGGAATCATGATTATAAGTCCTTCAGCCCTTGGACTC570.10283796705576705No Hit
AGCTGCGGGTATGAAGTAGCTTTTGTGTCATTCATGTCGATGACATGAAAGT570.10283796705576705No Hit
AATTGAGGGTCAGGGGATAACGGGCAAATGGTGAAACCCAGAAAGTTTTGTT570.10283796705576705No Hit
TATACAGGCTCCCTTAGCTGGCTCTAGGTTTTATTGTTTTTGTGTCTTTCCT570.10283796705576705No Hit
GCAACAGGGTTACCTTCCTGTGCAAAATCCTTTCATTGGCTTCTTGCTGGAA570.10283796705576705No Hit
TCTCATGGGACCCTTTACATTTTTGTATCATAATAGATTTTAAAAATCTAAT570.10283796705576705No Hit
GCGCGAGGGCTTTAGCTAGGTAGGAGGATATAGTTATAAAAGAAAAACAGGA570.10283796705576705No Hit
TTGTGCGGGACTTGAGGTTATGGGACTGCAAGATTCCAAATGTGCATTTCAA570.10283796705576705No Hit
CGAGCTGGGTGATTTCAATAACTGTGAAAAATAAAAAAGGTGTTGTATTGCT560.10103379219513955No Hit
TTATGGGGGAAACAGGAAGACTAAAAAAAGGAATATTGTTGAAACCAGCAGA560.10103379219513955No Hit
GCTTGAGGGCATATAAAGCTGAATACAAATTGGTTTGGGGGGAGATCCTTTC560.10103379219513955No Hit
AATCTGGGGAGCTTACGAGGAAATATTTCAGTAAAAGCAGTTAAAAAAGAAG560.10103379219513955No Hit
TAGCGAGGGCCACCCTCTGAGACTCCATTGGTGTCACACAAGGTGTCTTCTT560.10103379219513955No Hit
AAGAAGGGATTCGTCCCCACCCACGTCCTGGTGATTGGTCCATTTTACAGAG560.10103379219513955No Hit
GCGCGGGGACATTCTACTATTGAATTATGTACAACATTTTCATTTTTTATGC560.10103379219513955No Hit
GTTGCTGGGACATAATGCCAAATTATTCTTCATAAGAGCTGTTGCCAAATCA560.10103379219513955No Hit
TTCTCAGGGTACCTTTTCTGTTAACTATCACAAATTCCTAGAGAAACAAGAT560.10103379219513955No Hit
GCTGCAGGGGAATGCTAAAACACAGCTGGAATCTAAGAAGGGAAGAGGGCTT560.10103379219513955No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)