FastQCFastQC Report
Sat 31 Dec 2022
SRR5846075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71067
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC9271.3044028874161002No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT8951.2593749560274108No Hit
TGGCATGGGTACAGTCGGGCTCACTTAAACCTATGGAATCAGAAACTCTGGG2840.39962289107461973No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACT1600.2251396569434477No Hit
AAGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.18855446269013748No Hit
TTCTGTGGGCCTGGGCTTATGGCCGGTCACAGCCTCAGTGTGACTCCACAGT1330.18714733983424092No Hit
GGCTTTGGGGACGGTTAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGA1330.18714733983424092No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCTGTC1260.1772974798429651No Hit
GCTTGAGGGTTACTAAGCATGTGCCGTCGTAGGTATTAGTGCTAGTCTCAAA1260.1772974798429651No Hit
GCTAAAGGGGCCCGAGCCAGTGTGCTGAGCTCTCCGCGTCGCCTCTGTCGCC1250.17589035698706854No Hit
TCATTTGGGTCACTGAGAATATATTATTGGTATGAGACATTATTAAAATATG1250.17589035698706854No Hit
TCCATCAGGGGTACAATAGGTAATCAATAATTGGCCTCTTGGGACTGACTGT1210.17026186556348236No Hit
CTGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.17026186556348236No Hit
ATAGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.1688547427075858No Hit
ATACTAGGGTTACTGTTATCATGCAGTTCTGTCTCATAAAGAAATGGTAGGA1180.1660404969957927No Hit
GTCAATGGGACATAGTGAGTAGGGAAAATTGAGATAATGAAGAATAATGACT1180.1660404969957927No Hit
AATAATGGGATTAGCGCTCATCTTCATCATGGGCAACAGCGCCAAGGAAGCT1160.16322625128399962No Hit
AAATATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1150.16181912842810306No Hit
GACACAGGGGAGGGCGGGCGCGGGGTCTTGGCGAACGGTCTTCGGAAGCGGC1150.16181912842810306No Hit
AGTCGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.1604120055722065No Hit
CCAAGCGGGATTGTGGCGGGCGTGTTCATTTTCAGTGCTGTGCAGATGACGC1130.15900488271630997No Hit
ATTCAGGGGGAAGAAGGAAACAGTAGAGAGTAAGCAACTGAATGCAATGGGA1120.1575977598604134No Hit
ATCCAAGGGATACCGGGCCCTACAGGATGCTGAGTGGCATCCCTGGCCTTCA1120.1575977598604134No Hit
CATATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1090.1533763912927238No Hit
GATTTGGGGGGCCCACCAGGTGCCAGTGGTGAACAGTGTCTGATCATGTGAA1090.1533763912927238No Hit
TACAGGGGGAGTCCAACATGGCGGCGCCCAGCGGAGGGTGGAACGGCGTCGG1080.1519692684368272No Hit
GGTTTTGGGAACCTTGTCAGATGTTAACTTCTAAGTTCGGTTTGGGATTTTT1080.1519692684368272No Hit
CGAGTTGGGACAAAAACACTGGCACTTGAATGTTGAATGTCACCGTATGCGT1060.14915502272503411No Hit
ACGACAGGACCCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGAC1060.14915502272503411No Hit
CAACTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.14774789986913756No Hit
ATCGCCGGGATCGCAGCCTGCGAGGAGATGTTACGGCAGCTGGGCCGCCGGC1040.14634077701324102No Hit
GTAGACGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT1040.14634077701324102No Hit
CCTGAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.14634077701324102No Hit
GGTGTTGGGTGGAATAAAATAGAAATTACAGTTTGATAGGAATCCTAGAATC1020.14352653130144793No Hit
ACAGAAGGGTGCCAGGTGACATGCTAGGAGCTTCATATATCCCATTTCATTC1000.14071228558965482No Hit
ACGTATGGGATAGATTCCATTAGGAAAAGCAGAATGTTTGTGATCCTGTTCA1000.14071228558965482No Hit
CCATGTGGGATGGGAGTGGACATCCGCCATAACAAGGACCGAAAGGTTCGGC990.1393051627337583No Hit
AAGTATGGGGGTCTGTTCCATGTGACTTTACTCCATAAAGTGATTATAGGAC990.1393051627337583No Hit
ACAAACGGGAGGTAATAGAAAATATACACAAGTCAGAATGTGAAATTAAATA980.13789803987786176No Hit
TTTTGGGGGCTGGCCAGATGAGGGTGCCTCCCTCTACCACGTATATGAGGTG960.13508379416606864No Hit
AAGTCTGGGGACGCTCTTCCGATCTGCCTAAGGGCAGATGACTACGTAGGAA950.1336766713101721No Hit
CTTGGTGGGGTGCACTTCTATGACATGGAAATTATTGAAGAAGAAGCTTTCT940.13226954845427552No Hit
CGTGTTGGGCTTCTATATATTTCCAGCTTAAATTCCTCTTCCGAGCTCCAAA940.13226954845427552No Hit
TTCTTTGGGTCTGTCAGCTACCACCCTGTGCTTTAAAAATGCACACACTCAA930.130862425598379No Hit
TCATTTGGGCTTTCAAAACTGTGCTGAATTCAATGAGCCTGATTCAGAAGTA930.130862425598379No Hit
TGCGCGGGTGGCACGGGACCAAAAGGGAAGGTAAAAGAAAGCAAAGAAGACA930.130862425598379No Hit
ATCTTCGGGGTGCTTACATCACGAGAAAAAGCAGCCAAGGATTATAACCAGA930.130862425598379No Hit
GCTTAAGGGTTATCGAGCATCTAGCATGTTCCAGACACAATCTGAACACTGA920.12945530274248246No Hit
CCGACTGGGCCGAAGATCATGCAGAGAAGGCATCTGTGGCTCTTGTGCAATG920.12945530274248246No Hit
ACTTTAGGGGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCAC920.12945530274248246No Hit
TGCAAAGGGAAGGCTTGGAGACCTTCCAGTATCTCATGCCTTATTCTAGATC920.12945530274248246No Hit
ATGTACGGGACCTGCAAATGGCGCTTCCAGTACTGTGTTCAGTGATCCACAT920.12945530274248246No Hit
AACGCTGGGATGAGCTATGTTGCATAAGTTGATGGACTTCATTAATCAAATC920.12945530274248246No Hit
CATACTGGGAACCACAGGTCTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTT910.1280481798865859No Hit
GCTAATGGGGTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGTCGAG910.1280481798865859No Hit
TAAGGTAGGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC910.1280481798865859No Hit
CTCGCAGGGATCCTGCGGTGGCTGGCGTGCTGGGCATGGGGCCTGCTCACAG900.12664105703068934No Hit
TAGTGAGGGCAGACAATGTACCCAAGTGTGATGAGATTCTGATGGAAGAGAT900.12664105703068934No Hit
AGGCCAGGCTGGTCTCAGACTCCTGATCTCGGGTGATCTGCCCACCTCGGCC900.12664105703068934No Hit
ATCGCCAGGGTGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC900.12664105703068934No Hit
ATGTTGGTTTACCTGTAGTGTTTTATAAAGTGTGGTCAGAGGCCCCTATAGA880.12382681131889627No Hit
GTGGTTGGGCGATCTGTTCCTGGGCCACCTCAATGATATTTCTCTGAGAACA880.12382681131889627No Hit
GAACTAGGGAGGTTTGTAGACTGGAAGAACTAGGCTATACCATAGAGCCTAG880.12382681131889627No Hit
TATTAGGGGCTCTCCAAACATGAAAGTTAGCACATCTATTAAAAAGCAAATC880.12382681131889627No Hit
ACAGCTGGGCAAAAATGTATTGAAGCAACAAATATTTCCTGAAGATCCACCC880.12382681131889627No Hit
CATAATGGGAGCTAGATTCAAATGGGCTGATAACCAAATTTTAACACATCAG870.12241968846299969No Hit
CAAAATGGGATCTTTCTCTACTGTAAAGTGATTTTGTTTGCAGGTAAGAAAA860.12101256560710315No Hit
GATGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.12101256560710315No Hit
GTGCTATGGAGAGACTTTTATCTCTAAAATGAATTCCCTATACACATATGGA850.11960544275120662No Hit
TTAGTAGGGATAGCGATCTCCCAGAAACTTGGACTGGAATGCTCCTACACTG850.11960544275120662No Hit
CCAGATGGGGAAGGTGTGGGGTCTTTCCCAACCTGAGGATTTCTGAAAGGTT840.11819831989531007No Hit
ACGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.11819831989531007No Hit
TACTAGGGGGGGGGGCAAAATAGACATACAATAGGAAAAATAATAATAAAGA840.11819831989531007No Hit
TCATCTGGGGGAGGAAGCACTAAAGATGGAAAGGAAAAACCAGTACCAGCCA840.11819831989531007No Hit
GCGCACGGGAAGTGTTTGATTTATTGAAGGTGTATGCATATCCCAAGGCATG840.11819831989531007No Hit
TCGGACGGGCGATCTCTCATCGGGGGGCGCGTCGAGGGGAGAGGCAGCAGCC840.11819831989531007No Hit
TTCTCCGGGAGCCACAGGCGGACCTGCAGGCTCTGCTGCAGAGCGGTGCCCA840.11819831989531007No Hit
TAATGGGAAACATTTTTAAAATTAAAATGGATAATTTGTAAATAGTTTTTAG830.1167911970394135No Hit
GTCTGCGGGGTTGCATGTATTGTGATTTTGTTTGATCATTTGTGTGCATTTC820.11538407418351697No Hit
CTAATCTCGGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCTCTTTCCTG810.11397695132762042No Hit
TGAATGGGGGGCACCTGAGGTCCATAAGCGTGAAAAGAGGCAGGAAGAGAAG810.11397695132762042No Hit
CCAAAGGGGATCTTCCTCAATCGCTTTTTGGACCTGACCGATGCAATCGAGG800.11256982847172385No Hit
TGAGAACGGGGATTACAGCTACCTTCTAGCTTTAGTATGGTAGTGCTTCATT800.11256982847172385No Hit
GCGACAGGGAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCCA800.11256982847172385No Hit
CGGTGGGAAGGGTGTTGGTAAATGGGTCCTCTCATAGTCTCTTTGTGAGAGT800.11256982847172385No Hit
GAAAAAGGGGGGAAACTTGGATGCCCAGCCATTTTGGTGACCTGAGAGTCTA790.11116270561582732No Hit
ATGTAAGGGGAGGCCGCCTGTGGCCACCATGTCGGCCCTCTTTCCACCACCC790.11116270561582732No Hit
TATCATGGGATAGTCCAATGTTTCATCATCAGGAACTTTCGGTTTTAAAAAA790.11116270561582732No Hit
TGACCATGGGACCCATTACTGGATATATAACCCAAAGGATTATGAATCATTC780.10975558275993078No Hit
CCACCCCAGACCTCCTGAATCAGACACTCTGGGTGTGGGGCCCAGTGTTCTG770.10834845990403423No Hit
TGCAATGGGTATCAGAGGTGCTTCAAGGAAAACTAGGAATCTATGATGCTGA770.10834845990403423No Hit
AACCGGGGATGTTATTTATTCAGATCCTTTTTTTCCAGAAAGAAAAATTGTC770.10834845990403423No Hit
CACAATGGGACTGGTACCCTCAAACACAGGACTGAAGCTTAATTGGGAATTT760.10694133704813767No Hit
GTTATAGGGGAGAGCAAAATAACACAACATTGTTGTACACGGTTGAACACTT760.10694133704813767No Hit
CTATTTGGGGGAGAATACTTCGCCTAAAATACTGTTAAGTGGGTTAATTGAT760.10694133704813767No Hit
ACATGGGGGAGTCGATTTATATACTGACTTTTATTAACTATGACAGATGTAT760.10694133704813767No Hit
ACGAGAGGGGGGGGCCGGCAGGTGCTCCGCAGCCGTCTGTGCCACCCAGAGC760.10694133704813767No Hit
ATCGTAGGGATCACCATAGAGGATGTTTCAGATGTGGCAATTCCTTTAAATC750.10553421419224113No Hit
TGTTATGGGGTGGAAAAATGTTCTGTTTGTAGAACTCATAGTATTCACTTAT750.10553421419224113No Hit
GACTATGGGAATTGGCTGCAATTATTTAATGTTTAATTGGGTTGATCAAATG750.10553421419224113No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTA750.10553421419224113No Hit
TTCGTGGGGGTGTAACCTGATGTCTCAACCCCCTGTGAGAAGAGTCCATTAT750.10553421419224113No Hit
CTGTCTCGGGAGACTTTTTCTTGACCTTCGAATGAGCAGAATAAAGAGCATA750.10553421419224113No Hit
CTAGTTGGGGGGGCGGGCTTCATAGAGACTTGGGAATGAGCTGAAGGTGAAA750.10553421419224113No Hit
ACGCATGGGCCATATTCTATTACTCATTATTTTTTTTGTTTTGCACCCCAAA750.10553421419224113No Hit
AACTAGGGGCTTTCAGAAATTGTTATTTATGAAGACTCCCATTATCGTTTTT750.10553421419224113No Hit
AGACAGGGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATACCA750.10553421419224113No Hit
CGAACCGGGCAGGTGGTGAGGTATAAGTTACTTGCTCTTAGCATTTGGTCTG740.10412709133634458No Hit
TGAGTTGGGGGGAAATGCAAATCAAAATTACTATGCGATATCACCTCACACT740.10412709133634458No Hit
CAAGGGGATATGATGTACAGTAAGGTAATAAGAGTAATAAAAACAAGTTGCT740.10412709133634458No Hit
TCATACTGGGGAATCGCTAGCATAACTGATCACCTCATTGCCATGCTTGCTG740.10412709133634458No Hit
GCCAGTGGGATCACTCTAGAAGAATCTGTACATCATCAGGACTGCATTGCTC740.10412709133634458No Hit
TGAGGCGGGAAGAAATAAAAGACATACAAATTGTAAAGAAAGAGGTCAGACT740.10412709133634458No Hit
ATATAAGGGGGTTACTACATCTTCCGTGGGGCGAATTCCCAGAAAAACACTT730.10271996848044802No Hit
ATGCTAGGGCACATACATTCATATATCTCAAGAAATGAAAGCAATGAAAGAG730.10271996848044802No Hit
AAACAAGGGCCTATAGTATATTTTGAGTTAAGTTTTATGGTGTGAAGTAAGG730.10271996848044802No Hit
TCAGAATGGGAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCACAG730.10271996848044802No Hit
TAGCTGGGGGATTCCATTTATAAGTAATATCTAGAGTACGCAAATTCATAGA730.10271996848044802No Hit
TCGATAGGGACAGACGGTTTCACCATGTTGGTCAGGCTGGTCTTGAACTCCT730.10271996848044802No Hit
TCAAGAGGGCTGACCAAGCTGTAGGCCTCCACTGGCCCCATCACTGCCAACT730.10271996848044802No Hit
CTCCTTGGGGAGAATTCAATTTTAGATGGTCTCCAGAGCAGAATTTGATGTA730.10271996848044802No Hit
CTACTTCGGGGAATTGTCAAAAACCAGAATTAGGTCAAGTTCATAGTTTCTG720.10131284562455148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)