FastQCFastQC Report
Sat 31 Dec 2022
SRR5846065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230364
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC13670.5934086923303988No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT10000.4340956052160928No Hit
ATAGCGGGGAAAATTATCCAGGTTATATAATTATTAGATGTTGGACTCAATC3110.13500373322220485No Hit
CAAAGTGGAGATAAAATTGACTTTAAACCAACAACAGTAAAAAGGGGCAAAG3010.13066277717004393No Hit
AGCTGCGGGATTGTGTTTTGGAATGGAGTTGGAGTATTGAGTACCAGGCAGT2910.126321821117883No Hit
GCAAAAGGGAGAGACCTTGTGTGGCTGTCAAGCCCGAAATATTTACTATCTG2590.11243076175096803No Hit
GTAGCCGGGGCAGTAGGATACTCTCTCTCATTGCTGGGCAGCGGCAGGGAGC2570.11156257054053585No Hit
GCGATAGGGATGCCATACATGCTTATTACATATTTAATAGACTTTAGCTCAC2550.11069437933010366No Hit
GTGTCTGGGGCTTTCTCCCCGCCGCATTCCCGGTGTCGACTTACTAGCTGCA2490.1080898056988071No Hit
GAACATGGGGGTTAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGATAC2450.10635342327794273No Hit
TTTGATGGGCATTGTAGACTGATTTTTAAGGATATTTATCACAAGAATAGTT2380.10331475404143009No Hit
CGTTCAGGGAAGGCTTCCCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGC2340.10157837162056572No Hit
AGGCTTGGGGAAAAAAATATTACCACCTCTCTTTGTAATTTACTTTACTTGT2310.10027608480491744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)