FastQCFastQC Report
Sat 31 Dec 2022
SRR5846064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231866
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC13030.5619625128306867No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT9580.4131696755884865No Hit
CAAAGTGGAGATAAAATTGACTTTAAACCAACAACAGTAAAAAGGGGCAAAG3120.13456047889729414No Hit
ATAGCGGGGAAAATTATCCAGGTTATATAATTATTAGATGTTGGACTCAATC2790.12032812055238801No Hit
AGCTGCGGGATTGTGTTTTGGAATGGAGTTGGAGTATTGAGTACCAGGCAGT2530.10911474731094685No Hit
GTATGCGGGCCAAAGAAAATAATCAGCACTCCGTATGCTACCTCAGATCCTG2510.10825218013852829No Hit
TAAAAAGGGTCCTGCCATGGAAAAAAATGTAAGTTTCCATAAGTTCTTTAGA2510.10825218013852829No Hit
GCGATAGGGATGCCATACATGCTTATTACATATTTAATAGACTTTAGCTCAC2500.10782089655231901No Hit
AGCTGAGGGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCAC2470.10652704579369118No Hit
GAACATGGGGGTTAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGATAC2460.10609576220748192No Hit
CGTTCAGGGAAGGCTTCCCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGC2460.10609576220748192No Hit
AGGCTTGGGGAAAAAAATATTACCACCTCTCTTTGTAATTTACTTTACTTGT2450.10566447862127262No Hit
TATACCGGGGGCGGCGGCCCGGGCCGCGCGCCACCATGCTGGGCCTGGACGC2440.10523319503506336No Hit
GTGTCTGGGGCTTTCTCCCCGCCGCATTCCCGGTGTCGACTTACTAGCTGCA2410.10393934427643553No Hit
GTTTTAGGGTATATTATGAGTAACTGAAGAAACAAAACTAAATCAAAAAAAT2400.10350806069022625No Hit
GTAGCCGGGGCAGTAGGATACTCTCTCTCATTGCTGGGCAGCGGCAGGGAGC2400.10350806069022625No Hit
TGAGCCGGGCAGAAGAAGACAGGAAAATGTGGGAAGTTTGGAACCTCCTAGA2370.10221420993159842No Hit
GCAAAAGGGAGAGACCTTGTGTGGCTGTCAAGCCCGAAATATTTACTATCTG2340.1009203591729706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)