FastQCFastQC Report
Sat 31 Dec 2022
SRR5846061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81857
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC7750.9467730310175062No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT7720.9431081031555029No Hit
ACACATGGGCTTGCATTATTTGTGTGCTTTATGCTTTTTAGAAAATTTTTCC4220.5155331859217904No Hit
GGGCACGGGATTTCTGACCCATTTATTGGTATACTGTCACTTCCCTTTTTTT2500.3054106551669375No Hit
TCTCGGGGGCCGGCTGCAGGGATGGCGGGGAGGGGTAAGCTCATCGCAGTGA1650.2015710324101787No Hit
GCGTCAGGGGTGTCTCCCTAGCAAGATGGACACTGTGCAGGAAGGAAATGGG1440.17591653737615598No Hit
AGTCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1390.16980832427281725No Hit
TCCTAGGGGGAAATAATTCCAGCAAGGATCAGCAGGTGAAAGGTTTTTGGGG1360.166143396410814No Hit
TTGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1330.16247846854881073No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCTGTC1330.16247846854881073No Hit
CTCTTAGGGATAGAAACAAAGCAATTCCAGTAAGATATATAAAATATGTGCA1300.15881354068680748No Hit
TCTTCGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1270.15514861282480424No Hit
TTAGTTGGGAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA1250.15270532758346875No Hit
GACATGGGGATCCTGAGTCCTCAACAATTAAATAACTTGGACAAATTCACTG1250.15270532758346875No Hit
TTACTTGGGAAGAATCAGATTGGAGATAAAGAAAAGCTGGGAGGTAAACTTT1240.15148368496280099No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACT1230.15026204234213325No Hit
TTGCTGAGGGGTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTCAG1220.1490403997214655No Hit
GGAACTGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1220.1490403997214655No Hit
TACGCCGGGATTTTAAGTGCATTGAGGATAGATGCAGGCGGGTGAGCTGCCC1190.14537547185946223No Hit
TACGTGGGGGAAGAAGCACCAGGCCATGGAGGCCATGCTGCTGTACCTGGCC1170.14293218661812673No Hit
AGCTCCGGGAGATAAATGAGCAATTTCACAGATTCTTGCGGGAAAACTCAAA1170.14293218661812673No Hit
TAAAAAGGGTATACACTCCCACCAACAATGTATTAGCATTCTCTTATAGACT1160.14171054399745897No Hit
GCCATAGGGACTAATAAGGAGGTTCCAAGACTTCCCTTGGTTTAATGAGAAC1160.14171054399745897No Hit
TCAGCTGGGATTAGGCAAGAAACTGATTCACTGCAAAACTGGAAACCAAGGA1150.14048890137679124No Hit
TATTAAGGGTTCCTGGACCTGGTGTCCTGGTTGGGTCCAAGGTGATTTTACA1150.14048890137679124No Hit
TAGCTTGGGAGGCAGCACGTGGGCTCCAGCGCCGAGTTTATGGAGGCTTGGC1140.13926725875612347No Hit
GTCGAAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAAC1110.13560233089412024No Hit
AAGACGGGGCAGCCTCATGAGTAGCTGGGATTGCAGGCACGTGCCACCGCGC1110.13560233089412024No Hit
TTAAGGGGGACTTACCATGTGACTTTTTATTACCAAATGCATCAGTAGTGGA1110.13560233089412024No Hit
GAAGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1100.13438068827345248No Hit
AGAAATGGGGATCTCAAAAAGGGCCACGGGAAGCCCTGTGCTTGCCTGGGTC1100.13438068827345248No Hit
TGCGGTGGGAGAAATATGCAGGTCACTTGGATATTAGATAATGAGGGAAAAT1100.13438068827345248No Hit
CTACTGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1100.13438068827345248No Hit
TTACCTGGGACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAAT1080.13193740303211698No Hit
AGTAATGGGATACATTTCAAAGAGACTGAAAAATAATTTACAAGTTTTCTAA1050.12827247517011373No Hit
TGCCGAGGGACCAGCAATCTGTAACATCCCTAGAAAATTGTCTAGAACACAG1040.127050832549446No Hit
GTATTTGGGTGACTCCTTCAAGTGTTCAGTAGTACAATAGGTAATCAATAAT1040.127050832549446No Hit
TAGTTTGGGTCGTAGCTGGCATTTCAGGGTTTGTGTTTCGTCATGAGATCAA1040.127050832549446No Hit
AAGACCGGGCATTGGGAACACTGGAGAGTGGAATTCAGTGTAATTCAAGGCT1040.127050832549446No Hit
TGTAAGGGTGAGGGGTGACTACAGAATTTTTAAAAAGGAGAATGACATGACC1030.12582918992877823No Hit
GACAAAGGGATTACTATAGTAGCATATATCCAAAGATGAAATAAAATATTAC1030.12582918992877823No Hit
ATCTTAGGGGATCTTGCGAGGGGATGGCCATTTTCATGATATTGATTCTTCC1030.12582918992877823No Hit
TAAATAGGGAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGA1020.12460754730811048No Hit
GTCGACGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1020.12460754730811048No Hit
AATGGTGGGGAAAAAAATAGCTGGGCATGGTGGTACACGTCTGTAGTCCCAG1020.12460754730811048No Hit
TCCATAGGGCTTGAAATCTGTGGGAGGAAGGATCCTTTTTCACTCATTCAAC1010.12338590468744275No Hit
CCAACCGGCCGATCTGTGGATGGGAGATGGTCCAATGATGTCTTTCTTCTTC1010.12338590468744275No Hit
TTTGTCGGGCATGGTTGACAATTCTTTTCTTTCAGCAGCTGAAAAATTTGGG1000.12216426206677498No Hit
TACGCGGGGACTGGTGTTTATTACAGAGTTTGACATACATGGGTTCATTTGC1000.12216426206677498No Hit
ATGGCCGGGGATGTGGGCGGCCTTGGATGAGCAGATGCTGCAGGAGGGCATC1000.12216426206677498No Hit
TTCGCGGGGAATGGTAAATGGATCAATTCAACAAGAAGAGATAACTATCCTA990.12094261944610725No Hit
AATCAAGGGAAGATCTTTATAAGGAGAACTACAAAACATTGATGAAAGAAAT990.12094261944610725No Hit
GTTAAGGGGTGCCTTTAGTCCTCCAGTGTACCCTGCTGGAATTCTGCTTGTG990.12094261944610725No Hit
ATCTCAGGGATAGGATGGGGATGCAACTTTCAAGAGTTGCCTTGATTGGTAC980.11972097682543949No Hit
CATGTTGGGCTTACAGCCTAGTAGCAGTCATTAATTCAGCACAAGACGCTCC980.11972097682543949No Hit
GGGCTGGGGAGTTAAAGCCATCTACCGAGAAGTGTTAGTTCAGTTGGAAGTG970.11849933420477173No Hit
TCGCGCGGGGGGGAGCAGGTGGGCCCAGCCTGAGCGGGGCCTCTGCACAGCC970.11849933420477173No Hit
GAACTAGGGGGAAAAGATGGAAATGTAATGTTTAACTCAAATGAAAGGAAAT960.11727769158410399No Hit
GTGGCCGGGCAAAGGAACTTTCTATTGATCTTGAATGCAACACTGTTTCTCA960.11727769158410399No Hit
CTCAGTGGGAGTAATGATAGTTGTACAACAATGTGAATGTACTTAATGCCAC960.11727769158410399No Hit
TCTTATGGGGGACTTGGTTTCAGAAGATAGAAAGAACTTTCAAAACTCAATA960.11727769158410399No Hit
CTAGGCGGGATAAATCAAGCAGTAGTAAGATAGTGAGCATTCATACAAAAAT960.11727769158410399No Hit
AACGCTGGGATGCACCCAGAGTCCACTCTGTTCTTAGCACTTTGTACTGAAA960.11727769158410399No Hit
TGGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG960.11727769158410399No Hit
AGCATCGGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGA950.11605604896343623No Hit
GTGGTTGGGAGTGGTAGACTGGATAAAGAAAATGTAGTACATATACACCATG950.11605604896343623No Hit
GGCTTTGGGCACTCTATTTTATTTTTGTTGCCTGATGATATAATATAAAAAG940.1148344063427685No Hit
GAGGGTCTGTCTTCTCTAACACCTTGTGACCTTCTCTATATCATGCTTTAAA940.1148344063427685No Hit
ATCATAGGGATTCTGCATCAGTTTGCAGTTCACTGCAAATCTTTTACATTAA940.1148344063427685No Hit
ATGTCTGGAAAATACCATTTCTGCCTTTGAGTATTTCAGACCATTTTCTGTG930.11361276372210073No Hit
TATCAGGGGATTAGAGGATGGTACAGGTATTATGTAAGAATATATCAACAGT920.112391121101433No Hit
TGGTTCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA920.112391121101433No Hit
ATTATTGGGTGCTGCGCGTGCTCTCGCGCTTCGCGCCGCCCAACATGCAGGC920.112391121101433No Hit
ATGCTCGGGCGTGCACAGCGGGGAGAGACCCTTCCAGTGCCCAGAGTGCAAC920.112391121101433No Hit
TTGGTTGGGTAAGGAGAATTTGTTGCACTTACTTTCCTTTTAGTGTAGATCT910.11116947848076524No Hit
AGCTAAGGGTGAAGACCTTGGTATTAAATGCATGAGAAACCTTTTGCCATCT910.11116947848076524No Hit
CCGCTAGGGGATGCAGCATTTTGCTGCTACCTTACAAGCTTCTCTTCTGTCA900.1099478358600975No Hit
GATACAGGGGAGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG900.1099478358600975No Hit
GTTTATGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG900.1099478358600975No Hit
GTCTTTGGGTCGGGCCTGCCTCCTGCAAAAGAGGGGAAAGAGAAGAAGGAGA900.1099478358600975No Hit
GCATCAGGGATCTGTCCTTTGTTTTTGTGATTTACTAACAATCATCACGAAC890.10872619323942974No Hit
TATGCGGGAGCACCTATTAGGTTTCATTTTGCTGTTTTCAGGAATGTAAGAA880.107504550618762No Hit
TTATCGGGGAAGGTGACTGGGTGAGAGGAGGCTGGCCTCTTCGGACTGTGTT880.107504550618762No Hit
TCCGACAGGGTCTGTAGGCGGGAAGGAAAAATAGAGTATTATGGTTCAATGG880.107504550618762No Hit
CAATAAGGGATTAATGCAAAAGAAGGCAGAAAACAGGAAAAAGGAAACAAAG880.107504550618762No Hit
GCACCGGGGAGCCCTGCTTTAATGGACTTTTTCAGAAGTGTTTTTGCTTGTG880.107504550618762No Hit
GATAGCGGGAGGTGCTTCCTCTATATCAAGTGAGTTTACTGATTTTAAAATC880.107504550618762No Hit
CGTGACGGGCGTTCCCGAATGTGCTTTCCTCCCTCTCCCCTGCCCACCTCAA880.107504550618762No Hit
CAAATAGGGAAAAATGTGGAAGCAACTTTGTATCTCAGGTCTTAATGAACAC870.10628290799809424No Hit
TGGGACGGGCACCCAAAGATGTGAAGTTTTGAGCAATTTTTAAATCCCACTG870.10628290799809424No Hit
TAGCATGGGATGTGCATAAGTATTTAGCCAATACACATTTTGCTGACTTCTT870.10628290799809424No Hit
TACAAGCGGGGGCTAGTGGAAATCCTGAATTGCTTCCTTTCTGTGATCACTG870.10628290799809424No Hit
AAGACATGGGCCTGAGCTGCAAGGTTCTGCCTCACCTGTCTCTCCACTCTGA870.10628290799809424No Hit
AGCAAAGGGAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCA860.10506126537742648No Hit
GGTCCTGGGGACTTGCTATCAACAGTGTAACCCTACCTAACTTCCCTAATCC860.10506126537742648No Hit
AATGGTGGGAAATACATTGGACATTGGTTTCCAAATCTCCCTTTCTTCTTCA860.10506126537742648No Hit
AAAAGTGGGCAGAACTGAAAAGCTATGCATAGGACAAGGAAGATACATAGAA860.10506126537742648No Hit
GCTTCTGGGGAGGAATAATCTTTATTTAGGGTGAAAAGAAGAAGAGGATTCA860.10506126537742648No Hit
GATTCCGGGTAATGTCACTTCCAAGAAGAAAAACTGTTCTGTTGTAAAAAAT850.10383962275675875No Hit
TTCCATGGGATTTGTATGATATTTTAAAATCTCCTAAATGCTGGGCAATGGC850.10383962275675875No Hit
TGCATGGGGAATGTGGCAGAGGTGGTGGGAAGAAGAAAGTTGAGCTTTTTCC850.10383962275675875No Hit
TTCGTAGGGATCAAGGAATAGTTCAGAAAACGACTTCAGACCACAGAGACAG850.10383962275675875No Hit
CCAGTGGGGTACAGAGAGATCAGCTAAAAGATTTTTTACCGTTTATGGTTAA850.10383962275675875No Hit
AAAGTAGGGATTATTGATGACTAGACACCTGCTAGGATGTAAAGGCATTTAA840.10261798013609098No Hit
ACTGCTGTGCAACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCAC840.10261798013609098No Hit
TATTGCGGGCAGTGAAAATCTTTCTATGTTATTGCACTTTCCCCTAAGCCTT840.10261798013609098No Hit
TTAGCTGGGATGATGATCGCAGACAGGAAAGGAGGAAATTATGATAAAAAGT840.10261798013609098No Hit
CGTCAAGGGATCTGTAATACATTCTAAAAGGATAATTGTGTCTGGGGCTCAA840.10261798013609098No Hit
TGTAGTGGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAA840.10261798013609098No Hit
TTTCAAGGGATTAGTACTGTTACTCCTCATTGCACAGGTGATAATTACCAAT830.10139633751542325No Hit
CTCACAGGGCCTCGTTAGAAAGGTGGAAAGAGGAGAACGTAGCAATCTGGCT830.10139633751542325No Hit
TATTTGGGGTGCGAGGGGGAATCAGATTGGAGATAAAGAAAAGCTGGGAGGT830.10139633751542325No Hit
TCCATGGGGAACGTCGCCGATACTAATAAGTTGCAGTGTCTAGTTTCTCAAA830.10139633751542325No Hit
TAAAACGGGTTGCCCAGGCTGGAGTTCAGTGGCGTGGTCTCGGCTCGCTGCA830.10139633751542325No Hit
GTGCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC830.10139633751542325No Hit
GGTGATGGGAGAGAACTAGAAGCAGAGGGCAAGAAGCTCACGATGGACATGA820.10017469489475549No Hit
GTGGGGGGTGTTCTCTGAGGTCATAATTTCCTCGGAGACCTGTTTAACAAGC820.10017469489475549No Hit
GCCATGGGGATGTGCAAATGAATGCCACTTACCTCCATTCCACGGTATATAA820.10017469489475549No Hit
GGACTTGGGTCGGTGACTGGTTATCACACCATCGCTGGCCCCTTTGGGCCCT820.10017469489475549No Hit
GCTTAAGGGAAACAAAATGAAACTAGTTTGAAGTTGCAAAGTGTCTCCCCTC820.10017469489475549No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)